BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC124972.12 - phase: 0 /pseudo
(826 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC8758 similar to GB|AAC77862.2|20197419|AC005623 Argonaute (AGO... 512 e-146
TC14210 similar to PIR|A84668|A84668 Argonaute (AGO1)-like prote... 461 e-130
AV407626 213 1e-55
TC16141 weakly similar to GB|AAC77862.2|20197419|AC005623 Argona... 195 3e-50
AV421492 145 3e-35
TC18984 homologue to UP|AGO1_ARATH (O04379) Argonaute protein, p... 105 4e-23
BP052529 75 6e-14
BP056483 64 1e-10
TC9356 weakly similar to UP|AGOL_ARATH (Q9SJK3) Argonaute-like p... 59 3e-09
TC13170 similar to UP|Q9SDG8 (Q9SDG8) ESTs AU068544(C30430), par... 29 0.41
TC14409 similar to PIR|A84668|A84668 Argonaute (AGO1)-like prote... 32 0.53
TC10380 similar to UP|Q8S8U2 (Q8S8U2) Ycf1 protein, partial (40%) 30 2.0
AV408107 30 2.0
TC10574 similar to GB|AAF87136.1|9369388|AC002423 T23E23.3 {Arab... 30 2.0
TC15946 weakly similar to UP|Q43383 (Q43383) 2A6 protein, partia... 29 2.7
TC14740 similar to UP|Q8W584 (Q8W584) AT4g17230/dl4650c, partial... 29 2.7
TC12132 similar to UP|AAR14275 (AAR14275) Predicted protein, par... 29 3.5
TC7865 homologue to UP|SAHH_ARATH (O23255) Adenosylhomocysteinas... 28 4.5
AV409006 28 5.9
TC17495 membrane transporter [Lotus japonicus] 28 5.9
>TC8758 similar to GB|AAC77862.2|20197419|AC005623 Argonaute (AGO1)-like
protein {Arabidopsis thaliana;} , partial (32%)
Length = 1142
Score = 512 bits (1319), Expect = e-146
Identities = 258/341 (75%), Positives = 289/341 (84%), Gaps = 14/341 (4%)
Frame = +3
Query: 11 PPPPPIVPPNVKPIKIE-----QEHLKKKLPTKAPMARRGLGTKGAKLPLLTNHFEVNVA 65
PPPPPIVP +V+P+K++ E +KKKLPT+ P+AR+GLG+KG KLPLLTNHF+V VA
Sbjct: 120 PPPPPIVPADVEPVKVDLLDLPPEPVKKKLPTRLPIARKGLGSKGTKLPLLTNHFKVTVA 299
Query: 66 NTNRVFFQYSVALFYEDGRPVEGKGAGRKIIDKVQETYDSELNGKDLAYDGE-TLFTIGS 124
N++ FFQYSVAL YEDGRPVEGKG GRK+IDKVQETY SELNGKD AYDGE TLFTIGS
Sbjct: 300 NSDGHFFQYSVALSYEDGRPVEGKGVGRKVIDKVQETYGSELNGKDFAYDGEKTLFTIGS 479
Query: 125 LAQKKLEFIVVVEDVASNRNNANTSPD--------KKRIRKSYRSKTYKVEINFAKEIPL 176
LA+ KLEF VV+EDV SNRNN N SPD KKR+R+ Y SKT+KVEI+FA +IPL
Sbjct: 480 LARNKLEFTVVLEDVISNRNNGNCSPDGASTNDSDKKRMRRPYHSKTFKVEISFAAKIPL 659
Query: 177 QAIANALKGHEAENYQEAIRVLDIILRQHSAKQGCLLVRQNFFHNDPNNLNDVGGGVLSC 236
QAI NAL+G E+ENYQEAIRVLDIILRQH+AKQGCLLVRQ+FFHNDP N DVGGGVL C
Sbjct: 660 QAIVNALRGQESENYQEAIRVLDIILRQHAAKQGCLLVRQSFFHNDPKNYADVGGGVLGC 839
Query: 237 KGLHSSFRTTQSGLSLNIDVSTTMIVRPGPVVDFLIENQNVRDPFSLDWNKAKRTLKNLR 296
+G HSSFRTTQSGLSLNIDVSTTMI++PGPVVDFLI NQNVRDPFSLDW KAKRTLKNLR
Sbjct: 840 RGFHSSFRTTQSGLSLNIDVSTTMIIQPGPVVDFLIANQNVRDPFSLDWAKAKRTLKNLR 1019
Query: 297 ITAKPSNQEYKITGLSELSCKDQLFTMKKRGAVAGEDDTEE 337
I A PSNQEYKITGLSEL CK+Q FTMKK+G GE+D E
Sbjct: 1020IKASPSNQEYKITGLSELPCKEQTFTMKKKGGNNGEEDATE 1142
>TC14210 similar to PIR|A84668|A84668 Argonaute (AGO1)-like protein
[imported] - Arabidopsis thaliana
{Arabidopsis thaliana;} , partial (40%)
Length = 1525
Score = 461 bits (1187), Expect = e-130
Identities = 245/347 (70%), Positives = 275/347 (78%), Gaps = 35/347 (10%)
Frame = +2
Query: 494 GLVRDLIKCGGMKGILVEQP-KDVIEENRQFKGEPPVFRVEKMFADVLK-LSKRPSFLLC 551
GLVRDLIKC +KGI +++P +++ EEN QF+ PP+ RVEKMF + K L PSFLLC
Sbjct: 17 GLVRDLIKCARLKGIPIDEPYEEIFEENGQFRRAPPLVRVEKMFERIQKELPGAPSFLLC 196
Query: 552 LLPERKNSDLYGPWKKKNLAEFGIVTQCIAPTRVNDQYLTNVLLKINAKLGGMNSWLGVE 611
LLPERKNSDLYGPWKKKNLAE+GIVTQCI+PTRVNDQYLTNVL+KINAKLGG+NS LGVE
Sbjct: 197 LLPERKNSDLYGPWKKKNLAEYGIVTQCISPTRVNDQYLTNVLMKINAKLGGLNSVLGVE 376
Query: 612 HSRSIPIVSKVPTLILGMDVSHGSPGQPDIPSIAAVVSSRKWPLISKYRACVRTQGSKVE 671
+ SIPIVSKVPT+ILGMDVSHGSPGQ DIPSIAAVVSSR+WPLISKYRACVRTQ KVE
Sbjct: 377 MNPSIPIVSKVPTIILGMDVSHGSPGQSDIPSIAAVVSSREWPLISKYRACVRTQSPKVE 556
Query: 672 MIDNLFKPVSDKEDEGIIRELLLDFFHSSEERRPENIIIFRDGVSESQFNEVLNVELSQI 731
MIDNLFK VS+KEDEGIIRELL+DF+ SS +R+P+NIIIFRDGVSESQFN+VLN+EL+QI
Sbjct: 557 MIDNLFKQVSEKEDEGIIRELLIDFYSSSGKRKPDNIIIFRDGVSESQFNQVLNIELNQI 736
Query: 732 IEACKFLDENWNPKFMVIVAQKNHHTKFFQPRSPDNVPPAKFAILGIM------IFIC-- 783
IEACKFLDE WNPKF+VIVAQKNHHTKFFQP SPDNVPP I ++C
Sbjct: 737 IEACKFLDETWNPKFLVIVAQKNHHTKFFQPGSPDNVPPGTVIDNKICHPRNNDFYMCAH 916
Query: 784 -------------VLM------------LE*FLSYVYQRSTTAISVV 805
VL+ L LSYVYQRSTTAISVV
Sbjct: 917 AGMIGTSRPTHYHVLLDDIGFSPDELQELVHSLSYVYQRSTTAISVV 1057
>AV407626
Length = 426
Score = 213 bits (541), Expect = 1e-55
Identities = 103/144 (71%), Positives = 124/144 (85%)
Frame = +3
Query: 310 GLSELSCKDQLFTMKKRGAVAGEDDTEEITVYDYFVHRRKIDLQYSAGLPCINVGKPKRP 369
G SEL CK+Q F+++K+G DD+EE+ VYDYFV+ RKI+L+YS LPCINVGKPKRP
Sbjct: 3 GFSELPCKEQTFSLRKKG---DGDDSEEVAVYDYFVNTRKIELRYSGDLPCINVGKPKRP 173
Query: 370 TYIPIELCSLISLQRYTKALSTSQRSSLVEKSRQKPVERMRVLSNALKASNYGSEPMLRN 429
T+ PIELC L+SLQRYTKAL+T QRSSLVEKSRQKP ERM VL++ALK SNYG+EP+L+N
Sbjct: 174 TFFPIELCELVSLQRYTKALTTLQRSSLVEKSRQKPQERMNVLTDALKISNYGAEPLLKN 353
Query: 430 CGISITSEFTQVDGRVLQAPRLKF 453
CGISI++ FTQV+GRVL APRLKF
Sbjct: 354 CGISISNGFTQVEGRVLPAPRLKF 425
>TC16141 weakly similar to GB|AAC77862.2|20197419|AC005623 Argonaute
(AGO1)-like protein {Arabidopsis thaliana;} , partial
(14%)
Length = 598
Score = 195 bits (495), Expect = 3e-50
Identities = 102/173 (58%), Positives = 122/173 (69%), Gaps = 19/173 (10%)
Frame = +3
Query: 2 SSQENKECLPPPPPIVPPNVKPIKIEQ--------EHLKKKLPTKAPMARRGLGTKGAKL 53
+ +E LPPPPP++P +V P+K E+ E +KKK P + P+ARRGLGTKG K+
Sbjct: 78 NGKEESLPLPPPPPVLPSDVVPLKAEEVLPAAPPPEPVKKKTPARLPIARRGLGTKGTKI 257
Query: 54 PLLTNHFEVNVANTNRVFFQYSVALFYEDGRPVEGKGAGRKIIDKVQETYDSELNGKDLA 113
PLLTNHF+V N + FF YSV YEDGRPVEGKG GRKI+DKVQETY S+LNGKD A
Sbjct: 258 PLLTNHFKVTCTNNDGHFFHYSVNFTYEDGRPVEGKGVGRKIMDKVQETYQSDLNGKDFA 437
Query: 114 YDGE-TLFTIGSLAQKKLEFIVVVEDVASNRNNANTSP----------DKKRI 155
YDGE +LFTIGSL + KLE+ VV+EDV SNRNN N SP DKKR+
Sbjct: 438 YDGEKSLFTIGSLPRNKLEYEVVLEDVTSNRNNGNCSPPNGHGEDNETDKKRM 596
>AV421492
Length = 255
Score = 145 bits (365), Expect = 3e-35
Identities = 71/84 (84%), Positives = 79/84 (93%)
Frame = +2
Query: 627 LGMDVSHGSPGQPDIPSIAAVVSSRKWPLISKYRACVRTQGSKVEMIDNLFKPVSDKEDE 686
LGMDVSHGSPGQ DIPSIAAVVSSR+WPLISKYRACVRTQ +KVEMIDNLFK VS+KEDE
Sbjct: 2 LGMDVSHGSPGQTDIPSIAAVVSSREWPLISKYRACVRTQSAKVEMIDNLFKQVSEKEDE 181
Query: 687 GIIRELLLDFFHSSEERRPENIII 710
GI+RELLLDF+ SS +R+P+NIII
Sbjct: 182 GIMRELLLDFYLSSGKRKPDNIII 253
>TC18984 homologue to UP|AGO1_ARATH (O04379) Argonaute protein, partial
(22%)
Length = 698
Score = 105 bits (261), Expect = 4e-23
Identities = 60/143 (41%), Positives = 84/143 (57%), Gaps = 3/143 (2%)
Frame = +1
Query: 549 LLCLLPERKNSDLYGPWKKKNLAEFGIVTQCIAPTRV---NDQYLTNVLLKINAKLGGMN 605
L+ +LP+ N LYG K+ + G+V+QC V + QYL NV LKIN K+GG N
Sbjct: 10 LIVILPDN-NGSLYGDLKRICETDLGLVSQCCLTKHVFKMSKQYLANVALKINVKVGGRN 186
Query: 606 SWLGVEHSRSIPIVSKVPTLILGMDVSHGSPGQPDIPSIAAVVSSRKWPLISKYRACVRT 665
+ L SR IP++S PT+I G DV+H PG+ PSIAAVV+S+ +P I+KY V
Sbjct: 187 TVLVDALSRRIPLISDRPTIIFGADVTHPHPGEDSSPSIAAVVASQDYPEITKYAGLVCA 366
Query: 666 QGSKVEMIDNLFKPVSDKEDEGI 688
Q + E+I +LF ++ I
Sbjct: 367 QAHRQELIQDLFXTMARSSQRNI 435
Score = 70.9 bits (172), Expect = 8e-13
Identities = 36/82 (43%), Positives = 51/82 (61%)
Frame = +2
Query: 679 PVSDKEDEGIIRELLLDFFHSSEERRPENIIIFRDGVSESQFNEVLNVELSQIIEACKFL 738
PV G+I+ELL+ F ++ ++ P+ II +RDGVSE QF +VL EL I +AC L
Sbjct: 419 PVRGTLTGGMIKELLISFRRATGQK-PQRIIFYRDGVSEGQFYQVLLFELDAIRKACASL 595
Query: 739 DENWNPKFMVIVAQKNHHTKFF 760
+ N+ P +V QK HHT+ F
Sbjct: 596 EPNYQPPVTFVVVQKRHHTRLF 661
>BP052529
Length = 394
Score = 74.7 bits (182), Expect = 6e-14
Identities = 40/94 (42%), Positives = 61/94 (64%), Gaps = 4/94 (4%)
Frame = +3
Query: 13 PPPIVP--PNVKPI--KIEQEHLKKKLPTKAPMARRGLGTKGAKLPLLTNHFEVNVANTN 68
PPP+ P P+V P +E + +K + ++R+G+GT G ++P+LTN F+V+V +
Sbjct: 126 PPPLSPSSPDVSPSIETVEADPIKFSI-----ISRQGVGTAGKRIPVLTNLFKVSVNAPD 290
Query: 69 RVFFQYSVALFYEDGRPVEGKGAGRKIIDKVQET 102
+FFQYSV + ED R +E KG GRK+ID V +T
Sbjct: 291 AIFFQYSVTICMEDKRVIESKGIGRKLIDSVYQT 392
>BP056483
Length = 333
Score = 63.5 bits (153), Expect = 1e-10
Identities = 33/70 (47%), Positives = 48/70 (68%), Gaps = 7/70 (10%)
Frame = +1
Query: 6 NKECLPPPP--PIVPPNVKPIKIE-----QEHLKKKLPTKAPMARRGLGTKGAKLPLLTN 58
N++ +PP PIVP +V+P+ ++ E +KKKL A + R+GLG+KG KL LLTN
Sbjct: 124 NEDLMPPXXXXPIVPADVEPVNVDLLXXTPEPVKKKLTNLASIGRKGLGSKGTKLALLTN 303
Query: 59 HFEVNVANTN 68
HF+V VAN++
Sbjct: 304 HFKVTVANSD 333
>TC9356 weakly similar to UP|AGOL_ARATH (Q9SJK3) Argonaute-like protein
At2g27880, partial (10%)
Length = 697
Score = 58.9 bits (141), Expect = 3e-09
Identities = 45/154 (29%), Positives = 74/154 (47%), Gaps = 7/154 (4%)
Frame = +3
Query: 11 PPPPPIVPPNVKPIKIEQEHLKKKLPTKAPMA-----RRGLGTKGAKLPLLTNHFEVNVA 65
PPPP VP + ++EQ+ + + A R G G G K+ + NHF++ VA
Sbjct: 249 PPPPSPVPVDALSSEVEQKLALRPAAPSSTKAIRFPDRPGFGRLGKKIQVRANHFQLQVA 428
Query: 66 NTNRVFFQYSVALFYEDGRPVEGKGAGRKIIDKVQETYDSELNGKDL-AYDG-ETLFTIG 123
R Y VA+ E + K R+++ ++ + Y + G L +DG + LFT G
Sbjct: 429 E--RDLHHYDVAITPE----ITSKKVTREVVSQLIKMYKESVLGNRLPVFDGRKNLFTAG 590
Query: 124 SLAQKKLEFIVVVEDVASNRNNANTSPDKKRIRK 157
L EF+V +ED ++++ S +KR R+
Sbjct: 591 PLPFSSKEFVVKLEDDRPKASSSSGSKKEKRERQ 692
>TC13170 similar to UP|Q9SDG8 (Q9SDG8) ESTs AU068544(C30430), partial (14%)
Length = 588
Score = 29.3 bits (64), Expect(2) = 0.41
Identities = 14/15 (93%), Positives = 14/15 (93%)
Frame = +1
Query: 791 LSYVYQRSTTAISVV 805
LSYVY RSTTAISVV
Sbjct: 160 LSYVYPRSTTAISVV 204
Score = 21.2 bits (43), Expect(2) = 0.41
Identities = 9/15 (60%), Positives = 10/15 (66%)
Frame = +2
Query: 773 FAILGIMIFICVLML 787
+ I M FICVLML
Sbjct: 23 YVIPSTMTFICVLML 67
>TC14409 similar to PIR|A84668|A84668 Argonaute (AGO1)-like protein
[imported] - Arabidopsis thaliana
{Arabidopsis thaliana;} , partial (9%)
Length = 674
Score = 31.6 bits (70), Expect = 0.53
Identities = 15/15 (100%), Positives = 15/15 (100%)
Frame = +2
Query: 791 LSYVYQRSTTAISVV 805
LSYVYQRSTTAISVV
Sbjct: 44 LSYVYQRSTTAISVV 88
>TC10380 similar to UP|Q8S8U2 (Q8S8U2) Ycf1 protein, partial (40%)
Length = 5664
Score = 29.6 bits (65), Expect = 2.0
Identities = 12/42 (28%), Positives = 23/42 (54%)
Frame = +2
Query: 552 LLPERKNSDLYGPWKKKNLAEFGIVTQCIAPTRVNDQYLTNV 593
++ +++ D++ PWKKK + E + T P R +Y + V
Sbjct: 4403 IIEKKRRIDIFKPWKKKKILEVELET----PNRAKKEYTSRV 4516
>AV408107
Length = 415
Score = 29.6 bits (65), Expect = 2.0
Identities = 14/36 (38%), Positives = 18/36 (49%)
Frame = +3
Query: 729 SQIIEACKFLDENWNPKFMVIVAQKNHHTKFFQPRS 764
S I C L +NPK+ V+ + NHH F P S
Sbjct: 6 SSCICNCNNLSGRYNPKYGVVFSSFNHHHSPFSPSS 113
>TC10574 similar to GB|AAF87136.1|9369388|AC002423 T23E23.3 {Arabidopsis
thaliana;} , partial (19%)
Length = 733
Score = 29.6 bits (65), Expect = 2.0
Identities = 17/73 (23%), Positives = 37/73 (50%)
Frame = +3
Query: 490 CDVRGLVRDLIKCGGMKGILVEQPKDVIEENRQFKGEPPVFRVEKMFADVLKLSKRPSFL 549
C + L+ L + ++V++ + ++E Q + V VE++ + + K P FL
Sbjct: 183 CQAKELLDHLENVLANEPVVVKRGQHIVEVKPQGVSKGMV--VEELISTMRSEGKSPDFL 356
Query: 550 LCLLPERKNSDLY 562
LC+ +R + D++
Sbjct: 357 LCIGDDRSDEDMF 395
>TC15946 weakly similar to UP|Q43383 (Q43383) 2A6 protein, partial (56%)
Length = 1252
Score = 29.3 bits (64), Expect = 2.7
Identities = 11/30 (36%), Positives = 20/30 (66%)
Frame = -2
Query: 790 FLSYVYQRSTTAISVVKTYMQLLQSAMHTW 819
F+S VY+ +TT + + ++ +Q+L S H W
Sbjct: 255 FISEVYEGNTTQLGIFRSDVQVLVSMEHAW 166
>TC14740 similar to UP|Q8W584 (Q8W584) AT4g17230/dl4650c, partial (57%)
Length = 1406
Score = 29.3 bits (64), Expect = 2.7
Identities = 14/31 (45%), Positives = 20/31 (64%), Gaps = 6/31 (19%)
Frame = -2
Query: 282 SLDWN------KAKRTLKNLRITAKPSNQEY 306
SL WN K+T +N+++TAKP+ QEY
Sbjct: 1339 SLHWNI*RGKELNKKTKQNVKLTAKPTKQEY 1247
>TC12132 similar to UP|AAR14275 (AAR14275) Predicted protein, partial (14%)
Length = 698
Score = 28.9 bits (63), Expect = 3.5
Identities = 12/33 (36%), Positives = 18/33 (54%)
Frame = +2
Query: 10 LPPPPPIVPPNVKPIKIEQEHLKKKLPTKAPMA 42
+PPPPP P N P + HL+ + +P+A
Sbjct: 158 MPPPPP*QPTNPNPTRNYPIHLQPTTDSDSPLA 256
>TC7865 homologue to UP|SAHH_ARATH (O23255) Adenosylhomocysteinase
(S-adenosyl-L-homocysteine hydrolase) (AdoHcyase) ,
complete
Length = 1852
Score = 28.5 bits (62), Expect = 4.5
Identities = 14/31 (45%), Positives = 20/31 (64%)
Frame = -2
Query: 112 LAYDGETLFTIGSLAQKKLEFIVVVEDVASN 142
L +DG+ L T +L K ++FIV V +VA N
Sbjct: 1185 LRFDGDALHTRVALKTKHVDFIVKVTNVADN 1093
>AV409006
Length = 426
Score = 28.1 bits (61), Expect = 5.9
Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 5/35 (14%)
Frame = +3
Query: 10 LPPPPPIVPP-----NVKPIKIEQEHLKKKLPTKA 39
LPPPPP PP N P+ + ++ K+LP A
Sbjct: 201 LPPPPPPPPPHPDRRNTVPLLLCNQNSTKELPKSA 305
>TC17495 membrane transporter [Lotus japonicus]
Length = 979
Score = 28.1 bits (61), Expect = 5.9
Identities = 14/41 (34%), Positives = 22/41 (53%), Gaps = 2/41 (4%)
Frame = -3
Query: 4 QENKECLPPPPPIVPPNVKPIKIEQEHLKKK--LPTKAPMA 42
++N C+ P P P V+P I ++ L+ K +P AP A
Sbjct: 395 EKNTNCVKPEPKFPPAPVRPEMIPKDLLEMKGMIPNVAPQA 273
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.319 0.137 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,838,106
Number of Sequences: 28460
Number of extensions: 192364
Number of successful extensions: 1606
Number of sequences better than 10.0: 41
Number of HSP's better than 10.0 without gapping: 1446
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1569
length of query: 826
length of database: 4,897,600
effective HSP length: 98
effective length of query: 728
effective length of database: 2,108,520
effective search space: 1535002560
effective search space used: 1535002560
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 59 (27.3 bits)
Medicago: description of AC124972.12