BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC124967.4 - phase: 0 /pseudo
(335 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC9337 similar to UP|PME_PRUPE (Q43062) Pectinesterase PPE8B pre... 97 5e-21
TC17355 similar to UP|Q9FF77 (Q9FF77) Pectinesterase, partial (34%) 89 1e-19
TC9501 weakly similar to UP|Q9MBB6 (Q9MBB6) Pectin methylesteras... 68 2e-12
TC10949 similar to UP|PME3_PHAVU (Q43111) Pectinesterase 3 precu... 66 9e-12
BP070784 56 1e-08
TC19984 similar to UP|Q9MBB6 (Q9MBB6) Pectin methylesterase , pa... 55 1e-08
TC8690 similar to UP|Q8RVX1 (Q8RVX1) Pectin methylesterase precu... 54 3e-08
AV426815 50 4e-07
AU089161 47 3e-06
BP039243 46 8e-06
CN825195 43 7e-05
BP033848 43 8e-05
TC15529 homologue to UP|Q8LAH3 (Q8LAH3) Pectin methyl-esterase-l... 37 0.005
TC17425 weakly similar to UP|Q9FM79 (Q9FM79) Similarity to pecti... 33 0.052
AU089374 30 0.57
AI967495 30 0.57
TC17426 similar to UP|Q9SX30 (Q9SX30) F24J5.9, partial (29%) 30 0.57
BP055471 28 2.8
BP047783 28 2.8
AU088824 27 3.7
>TC9337 similar to UP|PME_PRUPE (Q43062) Pectinesterase PPE8B precursor
(Pectin methylesterase) (PE) , partial (54%)
Length = 1145
Score = 96.7 bits (239), Expect = 5e-21
Identities = 71/249 (28%), Positives = 124/249 (49%), Gaps = 11/249 (4%)
Frame = +2
Query: 57 RKDGTGDFTTVTDAVKSIPSGNKRRVVVWIGMGEYREKITVDRSKRFVTFYGERNGKDND 116
R DG+G++TTV +AV + P + +R V+++ G Y E + + K + G+ D
Sbjct: 23 RLDGSGNYTTVMEAVLAAPDYSMKRFVIYVKKGVYEEYAEIKKKKWNIMMVGD----GMD 190
Query: 117 MMPIITYDATALRYGTLDSATVAVDADYFVAVNVAFVNSSPMPDENSVGGQALAMRISGD 176
+ I + + T SAT AV F+A + F N++ P+++ QA+A+R D
Sbjct: 191 VTVISGNRSFGDGWTTFKSATFAVSGRGFIARGITFQNTAG-PEKH----QAVALRSDSD 355
Query: 177 KAAFYNCKFIGFQDTLCDDYGKHFFKDCFIQGTYDFIFGNGKSIYLRTTIESVAKGFECH 236
A F+ C G+QD+L + F+K+C I GT DFIFG+ +++ I++ KG +
Sbjct: 356 LAVFFRCSITGYQDSLYPHTMRQFYKECRITGTVDFIFGDATAVFQNCNIQA-RKGLQDQ 532
Query: 237 HST---R*GKNVGRHWIHFCS-LQHN-------WKWSQEHLPWSWLEEEP*GCICLYLYG 285
+T + K+ G+ F S LQ++ + W ++PW +E ++
Sbjct: 533 KNTITAQGRKDPGQSSGFFVSVLQYHCRF*TSTFSWFHPNVPWKTVEGVFKNSFHAIVHE 712
Query: 286 LCRQFQRMV 294
C +F R+V
Sbjct: 713 *CGEFTRVV 739
>TC17355 similar to UP|Q9FF77 (Q9FF77) Pectinesterase, partial (34%)
Length = 848
Score = 88.6 bits (218), Expect(2) = 1e-19
Identities = 54/172 (31%), Positives = 93/172 (53%), Gaps = 4/172 (2%)
Frame = +1
Query: 59 DGTGDFTTVTDAVKSIPSGNKRR---VVVWIGMGEYREKITVDRSKRFVTFYGERNGKDN 115
+GT ++T++ DA+ + P+ K + ++++ G Y E + V + K+ + G+ K
Sbjct: 172 NGTDNYTSIGDAIAAAPNHTKPQDGYFLIYVREGYYEEYVIVPKEKKNILLVGDGINKT- 348
Query: 116 DMMPIITYDATALR-YGTLDSATVAVDADYFVAVNVAFVNSSPMPDENSVGGQALAMRIS 174
IIT + + + + T +S+T AV + F AV+V F N++ QA+A+R +
Sbjct: 349 ----IITGNHSVIDGWTTFNSSTFAVSGERFTAVDVTFRNTA-----GPAKHQAVAVRNN 501
Query: 175 GDKAAFYNCKFIGFQDTLCDDYGKHFFKDCFIQGTYDFIFGNGKSIYLRTTI 226
D + FY C F G+QDTL + F+++C I GT DFIFGN ++ I
Sbjct: 502 ADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAVVFQNCNI 657
Score = 23.9 bits (50), Expect(2) = 1e-19
Identities = 11/25 (44%), Positives = 14/25 (56%)
Frame = +3
Query: 234 ECHHSTR*GKNVGRHWIHFCSLQHN 258
EC HSTR ++ HW +F HN
Sbjct: 687 ECCHSTRQNRSESEHW-NFNPKLHN 758
>TC9501 weakly similar to UP|Q9MBB6 (Q9MBB6) Pectin methylesterase ,
partial (29%)
Length = 934
Score = 68.2 bits (165), Expect = 2e-12
Identities = 35/95 (36%), Positives = 54/95 (56%)
Frame = +2
Query: 132 TLDSATVAVDADYFVAVNVAFVNSSPMPDENSVGGQALAMRISGDKAAFYNCKFIGFQDT 191
T+ +AT A A F+A + F N++ G QA+A+R GD++AF C +G+QDT
Sbjct: 56 TMQTATFANQAPGFIAKAITFENTA-----GPNGHQAVALRNQGDQSAFVGCHILGYQDT 220
Query: 192 LCDDYGKHFFKDCFIQGTYDFIFGNGKSIYLRTTI 226
L + F+++C I GT DFIFG ++ + I
Sbjct: 221 LYVQTNRQFYRNCVISGTIDFIFGTSPTLIQHSII 325
>TC10949 similar to UP|PME3_PHAVU (Q43111) Pectinesterase 3 precursor
(Pectin methylesterase 3) (PE 3) , partial (34%)
Length = 773
Score = 65.9 bits (159), Expect = 9e-12
Identities = 29/55 (52%), Positives = 37/55 (66%)
Frame = +3
Query: 167 QALAMRISGDKAAFYNCKFIGFQDTLCDDYGKHFFKDCFIQGTYDFIFGNGKSIY 221
QA+AMR D++ FY C F GFQDTL + F+++C I GT DFIFGN SI+
Sbjct: 12 QAVAMRSGSDRSVFYRCSFDGFQDTLYAHSNRQFYRECDITGTIDFIFGNAASIF 176
>BP070784
Length = 406
Score = 55.8 bits (133), Expect = 1e-08
Identities = 21/37 (56%), Positives = 30/37 (80%)
Frame = -2
Query: 185 FIGFQDTLCDDYGKHFFKDCFIQGTYDFIFGNGKSIY 221
F+G QDTL D G+H++KDC+I+G+ DFIFGN S++
Sbjct: 405 FLGAQDTLYDQVGRHYYKDCYIEGSVDFIFGNALSLF 295
Score = 38.9 bits (89), Expect = 0.001
Identities = 21/53 (39%), Positives = 32/53 (59%)
Frame = -3
Query: 278 CICLYLYGLCRQFQRMVPPWVQ*AVRLNYKRILSDEEAKHFLSMAYIHGEQWV 330
C+ YG Q+Q P A R+++ R L+DEEAK F+S++YI G +W+
Sbjct: 299 CLRTVFYG---QYQ-CSGPGASFAGRVSWSRELTDEEAKPFISLSYIDGSEWI 153
>TC19984 similar to UP|Q9MBB6 (Q9MBB6) Pectin methylesterase , partial (11%)
Length = 300
Score = 55.5 bits (132), Expect = 1e-08
Identities = 26/60 (43%), Positives = 40/60 (66%)
Frame = +3
Query: 54 VRVRKDGTGDFTTVTDAVKSIPSGNKRRVVVWIGMGEYREKITVDRSKRFVTFYGERNGK 113
V V KDG+GDF T+++A+ IP+ K R VV++ G Y E++TV + + VT YG+ + K
Sbjct: 120 VTVAKDGSGDFKTISEALNKIPATYKGRYVVYVKEGVYDEQVTVTKKMQNVTMYGDGSQK 299
>TC8690 similar to UP|Q8RVX1 (Q8RVX1) Pectin methylesterase precursor
(Pectinesterase) , partial (59%)
Length = 1082
Score = 54.3 bits (129), Expect = 3e-08
Identities = 37/98 (37%), Positives = 51/98 (51%), Gaps = 1/98 (1%)
Frame = +3
Query: 54 VRVRKDGTGDFTTVTDAVKSIPSGNKRRVVVWIGMGEYREKITVDRSKRFVTFYGERNGK 113
V V KDG+G F TV +AV S P K R V+++ G Y+E I + + K V G+ G
Sbjct: 804 VVVAKDGSGRFKTVAEAVASAPDSGKTRYVIYVKKGTYKENIEIGKKKTNVMLTGD--GM 977
Query: 114 DNDMMPIITYDATALRYG-TLDSATVAVDADYFVAVNV 150
D IIT + + T SATVA D F+A ++
Sbjct: 978 D---ATIITGNLNVIDGSTTFKSATVAAVGDGFIAQDI 1082
>AV426815
Length = 391
Score = 50.4 bits (119), Expect = 4e-07
Identities = 38/105 (36%), Positives = 50/105 (47%), Gaps = 1/105 (0%)
Frame = +3
Query: 37 ITLLEEHLTAAETTVRVVRVRKDGTGDFTTVTDAVKSIPSGNKRRVVVWIGMGEYREKIT 96
I+ + L A T V V DG+G+F TV+ AV + P +K R V+ I G YRE +
Sbjct: 75 ISAADRRLLQAGTVKADVVVAADGSGNFKTVSAAVAAAPEKSKTRYVIKIKAGVYRENVE 254
Query: 97 VDRSKRFVTFYGERNGKDNDMMPIITYDATALRYG-TLDSATVAV 140
V + K + F G D IIT + T SATVAV
Sbjct: 255 VPKKKTNIMFLG-----DGRTTTIITGSRNVVDGSTTFHSATVAV 374
>AU089161
Length = 400
Score = 47.4 bits (111), Expect = 3e-06
Identities = 19/40 (47%), Positives = 28/40 (69%)
Frame = +2
Query: 176 DKAAFYNCKFIGFQDTLCDDYGKHFFKDCFIQGTYDFIFG 215
D++AF++C G+QDTL + F+++C I GT DFIFG
Sbjct: 263 DRSAFFDCAISGYQDTLYAHAHRQFYRNCEISGTVDFIFG 382
>BP039243
Length = 462
Score = 46.2 bits (108), Expect = 8e-06
Identities = 24/58 (41%), Positives = 32/58 (54%)
Frame = -2
Query: 56 VRKDGTGDFTTVTDAVKSIPSGNKRRVVVWIGMGEYREKITVDRSKRFVTFYGERNGK 113
V KDGTG+F + +AV + P+ R V+ I G Y E + V R K V F G+ GK
Sbjct: 434 VAKDGTGNFAPIGEAVAAAPNSRATRFVIHIKAGAYFENVEVIRKKTNVMFVGDGIGK 261
>CN825195
Length = 551
Score = 43.1 bits (100), Expect = 7e-05
Identities = 20/61 (32%), Positives = 32/61 (51%), Gaps = 1/61 (1%)
Frame = +2
Query: 54 VRVRKDGTGDFTTVTDAVKSIPSGNKRRVVVWIGMGEYRE-KITVDRSKRFVTFYGERNG 112
V V KDG G T+ +A+K +P RR ++++ G Y E + + R + V G+ G
Sbjct: 278 VVVSKDGNGTAKTIAEAIKKVPEYGSRRFIIYVRAGRYEENNLKIGRKRTNVMIIGDGKG 457
Query: 113 K 113
K
Sbjct: 458 K 460
>BP033848
Length = 525
Score = 42.7 bits (99), Expect = 8e-05
Identities = 19/44 (43%), Positives = 27/44 (61%)
Frame = +2
Query: 56 VRKDGTGDFTTVTDAVKSIPSGNKRRVVVWIGMGEYREKITVDR 99
V KDG+G F T+ A+ S P G K R ++++ G Y E ITV +
Sbjct: 377 VAKDGSGKFKTIASAIASYPKGFKGRYIIYVRAGVYDEYITVPK 508
>TC15529 homologue to UP|Q8LAH3 (Q8LAH3) Pectin methyl-esterase-like
protein, partial (5%)
Length = 548
Score = 37.0 bits (84), Expect = 0.005
Identities = 15/33 (45%), Positives = 22/33 (66%)
Frame = +1
Query: 303 RLNYKRILSDEEAKHFLSMAYIHGEQWVRPPPK 335
R Y + LSD + K F+++A I G +W+ PPPK
Sbjct: 139 RPEYVKQLSDADVKPFITLASIEGSKWLLPPPK 237
>TC17425 weakly similar to UP|Q9FM79 (Q9FM79) Similarity to pectin
methylesterase, partial (33%)
Length = 643
Score = 33.5 bits (75), Expect = 0.052
Identities = 13/29 (44%), Positives = 20/29 (68%)
Frame = +1
Query: 303 RLNYKRILSDEEAKHFLSMAYIHGEQWVR 331
R+ + + LS EEA+ FL YI G++W+R
Sbjct: 331 RVEWSKSLSSEEARPFLGREYISGDEWLR 417
>AU089374
Length = 694
Score = 30.0 bits (66), Expect = 0.57
Identities = 12/33 (36%), Positives = 20/33 (60%)
Frame = +3
Query: 242 GKNVGRHWIHFCSLQHNWKWSQEHLPWSWLEEE 274
GK++GRHW+ + S S+ H+P S+L +
Sbjct: 273 GKSIGRHWMSYLSPLGKPTQSEYHIPRSYLNSK 371
>AI967495
Length = 390
Score = 30.0 bits (66), Expect = 0.57
Identities = 11/24 (45%), Positives = 15/24 (61%)
Frame = +1
Query: 198 KHFFKDCFIQGTYDFIFGNGKSIY 221
+ F+K C I GT D IFGN ++
Sbjct: 28 RQFYKQCQIYGTVDLIFGNAAVVF 99
>TC17426 similar to UP|Q9SX30 (Q9SX30) F24J5.9, partial (29%)
Length = 999
Score = 30.0 bits (66), Expect = 0.57
Identities = 10/27 (37%), Positives = 17/27 (62%)
Frame = -2
Query: 242 GKNVGRHWIHFCSLQHNWKWSQEHLPW 268
G ++G+ WI F S H W +S++ + W
Sbjct: 401 GSSLGKTWIFFLSSSHAWIYSRDPILW 321
>BP055471
Length = 413
Score = 27.7 bits (60), Expect = 2.8
Identities = 11/24 (45%), Positives = 17/24 (70%)
Frame = -1
Query: 39 LLEEHLTAAETTVRVVRVRKDGTG 62
+LE+H T + T+R + V K+GTG
Sbjct: 335 ILEDHRTTGKATIRAMLV*KNGTG 264
>BP047783
Length = 526
Score = 27.7 bits (60), Expect = 2.8
Identities = 12/33 (36%), Positives = 18/33 (54%)
Frame = +1
Query: 180 FYNCKFIGFQDTLCDDYGKHFFKDCFIQGTYDF 212
F+ C+ + F+D Y K F+D FI+ Y F
Sbjct: 406 FHYCRSLRFEDKPDYSYLKRLFRDLFIREGYQF 504
>AU088824
Length = 421
Score = 27.3 bits (59), Expect = 3.7
Identities = 21/88 (23%), Positives = 34/88 (37%), Gaps = 3/88 (3%)
Frame = +1
Query: 132 TLDSATVAVDADYFVAVNVAFVNSSPMPDENSVGGQALAM---RISGDKAAFYNCKFIGF 188
T+ +AT A A F+A + F EN+ G + G ++AF +G
Sbjct: 85 TMQTATFANQAPGFIAKAITF--------ENTAGPTVTKLWH*XTKGXQSAFRWAAHLGL 240
Query: 189 QDTLCDDYGKHFFKDCFIQGTYDFIFGN 216
L F++ CF+ + GN
Sbjct: 241 PKXLLVQTTXQFYRXCFLXRXH*IFLGN 324
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.328 0.141 0.457
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,727,671
Number of Sequences: 28460
Number of extensions: 112215
Number of successful extensions: 751
Number of sequences better than 10.0: 49
Number of HSP's better than 10.0 without gapping: 742
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 747
length of query: 335
length of database: 4,897,600
effective HSP length: 91
effective length of query: 244
effective length of database: 2,307,740
effective search space: 563088560
effective search space used: 563088560
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 55 (25.8 bits)
Medicago: description of AC124967.4