BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC124966.9 - phase: 0 /pseudo
(86 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC18079 weakly similar to UP|UFO5_MANES (Q40287) Flavonol 3-O-gl... 69 1e-13
TC16353 weakly similar to UP|HQGT_ARATH (Q9M156) Probable hydroq... 66 1e-12
TC9485 similar to UP|Q8S999 (Q8S999) Glucosyltransferase-10, par... 61 4e-11
AW428786 57 4e-10
CB829602 57 6e-10
TC9836 weakly similar to UP|Q9LXV0 (Q9LXV0) Glucosyltransferase-... 55 2e-09
TC9079 weakly similar to UP|Q8W4G1 (Q8W4G1) UDP-glucose glucosyl... 53 1e-08
TC15956 weakly similar to UP|LGT_CITUN (Q9MB73) Limonoid UDP-glu... 52 2e-08
TC17647 weakly similar to UP|AAR06918 (AAR06918) UDP-glycosyltra... 47 6e-07
TC17030 similar to UP|Q8S995 (Q8S995) Glucosyltransferase-14, pa... 45 2e-06
AV427981 43 9e-06
TC9717 weakly similar to UP|Q9LNI1 (Q9LNI1) F6F3.22 protein, par... 40 1e-04
TC9718 weakly similar to UP|Q9FI96 (Q9FI96) Glucuronosyl transfe... 40 1e-04
BI421006 34 0.004
AV768184 32 0.020
TC16492 weakly similar to UP|AAR06919 (AAR06919) UDP-glycosyltra... 31 0.034
TC16452 similar to UP|Q8LPJ3 (Q8LPJ3) Alpha-mannosidase, partial... 28 0.38
BP060297 27 0.64
TC17057 similar to UP|VATD_ARATH (Q9XGM1) Vacuolar ATP synthase ... 27 0.84
TC16717 weakly similar to UP|Q41419 (Q41419) Sucrolytic enzyme/f... 25 1.9
>TC18079 weakly similar to UP|UFO5_MANES (Q40287) Flavonol
3-O-glucosyltransferase 5 (UDP-glucose flavonoid
3-O-glucosyltransferase 5) , partial (33%)
Length = 889
Score = 69.3 bits (168), Expect = 1e-13
Identities = 34/65 (52%), Positives = 44/65 (67%), Gaps = 1/65 (1%)
Frame = +3
Query: 23 GPIIDTVTCSDRDANGL-ECLSWLDKQQSCSVLYVSFGSGGTLSHEQIVQLALGLELSNQ 81
GP++ TV R E L WLDKQ + SV+YVSFGSGGT+ Q+ ++ALGLELS
Sbjct: 3 GPLVRTVESKPRHGEDQDEILRWLDKQPAGSVIYVSFGSGGTMPQGQMTEIALGLELSQH 182
Query: 82 KFLWV 86
+F+WV
Sbjct: 183RFVWV 197
>TC16353 weakly similar to UP|HQGT_ARATH (Q9M156) Probable hydroquinone
glucosyltransferase (Arbutin synthase) , partial (52%)
Length = 885
Score = 66.2 bits (160), Expect = 1e-12
Identities = 33/65 (50%), Positives = 40/65 (60%)
Frame = +1
Query: 1 MEMGPIKAPTEEGSGNPSVYPVGPIIDTVTCSDRDANGLECLSWLDKQQSCSVLYVSFGS 60
+E G IK +E G P VYPVGP++ G ECLSWLD+Q SVL+V FGS
Sbjct: 685 LEPGAIKELQKEEPGKPRVYPVGPLVKVDGVQAGSGPGSECLSWLDEQPHGSVLFVCFGS 864
Query: 61 GGTLS 65
GGTL+
Sbjct: 865 GGTLT 879
>TC9485 similar to UP|Q8S999 (Q8S999) Glucosyltransferase-10, partial (75%)
Length = 1195
Score = 60.8 bits (146), Expect = 4e-11
Identities = 36/82 (43%), Positives = 49/82 (58%), Gaps = 12/82 (14%)
Frame = +1
Query: 17 PSVYPVGPI---IDTVTCSDRDANGL---------ECLSWLDKQQSCSVLYVSFGSGGTL 64
P VY +GP+ I VT D++ N L ECL WLD ++ SV+YV+FGS +
Sbjct: 811 PPVYSIGPLHLLIKDVT--DKNLNSLGSNLWKEDSECLKWLDTKEPNSVVYVNFGSITVM 984
Query: 65 SHEQIVQLALGLELSNQKFLWV 86
+ EQ+V+ A GL SN+ FLWV
Sbjct: 985 TSEQMVEFAWGLANSNKTFLWV 1050
>AW428786
Length = 322
Score = 57.4 bits (137), Expect = 4e-10
Identities = 29/67 (43%), Positives = 41/67 (60%), Gaps = 1/67 (1%)
Frame = +2
Query: 21 PVGPIIDTVTCSDRD-ANGLECLSWLDKQQSCSVLYVSFGSGGTLSHEQIVQLALGLELS 79
P+GP+++ + + CL WLD+Q SV+YVSFGS TL Q +LAL L+L
Sbjct: 101 PIGPLMENYSNKTSFWEEDVACLEWLDQQPPQSVIYVSFGSLATLEQSQFNELALALDLL 280
Query: 80 NQKFLWV 86
++ FLWV
Sbjct: 281 DKPFLWV 301
>CB829602
Length = 542
Score = 57.0 bits (136), Expect = 6e-10
Identities = 25/47 (53%), Positives = 35/47 (74%)
Frame = +1
Query: 40 ECLSWLDKQQSCSVLYVSFGSGGTLSHEQIVQLALGLELSNQKFLWV 86
ECL WLD Q+ SVLYV+FGS ++ +Q+V+LA G+ S +KF+WV
Sbjct: 91 ECLKWLDSQEPSSVLYVNFGSVINMTPQQLVELAWGIANSKKKFIWV 231
>TC9836 weakly similar to UP|Q9LXV0 (Q9LXV0) Glucosyltransferase-like
protein, partial (55%)
Length = 1286
Score = 55.1 bits (131), Expect = 2e-09
Identities = 29/75 (38%), Positives = 42/75 (55%), Gaps = 4/75 (5%)
Frame = +1
Query: 16 NPSVYPVGPIIDTVTCSDRDANG----LECLSWLDKQQSCSVLYVSFGSGGTLSHEQIVQ 71
N + +GP++ + R G C WLD + S SVL+VSFGS T+S Q++Q
Sbjct: 292 NRPAWAIGPVLLSTGSGSRGKGGGISPELCRKWLDTKPSNSVLFVSFGSMNTISASQMMQ 471
Query: 72 LALGLELSNQKFLWV 86
LA L+ S + F+WV
Sbjct: 472 LATALDRSGRNFIWV 516
>TC9079 weakly similar to UP|Q8W4G1 (Q8W4G1) UDP-glucose
glucosyltransferase, partial (65%)
Length = 1585
Score = 52.8 bits (125), Expect = 1e-08
Identities = 26/80 (32%), Positives = 44/80 (54%), Gaps = 10/80 (12%)
Frame = +1
Query: 17 PSVYPVGPIIDTVTCSDRDA----------NGLECLSWLDKQQSCSVLYVSFGSGGTLSH 66
PS+YP+GP + + ++ +CL WL+ ++ SV+YV+FGS +S
Sbjct: 742 PSLYPIGPFPSFLNQAPQNQLASLGSNLWKEDTKCLQWLESKEPGSVVYVNFGSITVMSP 921
Query: 67 EQIVQLALGLELSNQKFLWV 86
EQ+++ A GL + FLW+
Sbjct: 922 EQLLEFAWGLANGKKPFLWI 981
>TC15956 weakly similar to UP|LGT_CITUN (Q9MB73) Limonoid
UDP-glucosyltransferase (Limonoid glucosyltransferase)
(Limonoid GTase) (LGTase) , partial (65%)
Length = 1169
Score = 51.6 bits (122), Expect = 2e-08
Identities = 27/73 (36%), Positives = 39/73 (52%), Gaps = 5/73 (6%)
Frame = +2
Query: 19 VYPVGPIIDTVTCSDRDANGL-----ECLSWLDKQQSCSVLYVSFGSGGTLSHEQIVQLA 73
+ PVGP+ + G +C+ WL+ + SV+YVSFGS L EQ+ ++A
Sbjct: 734 IRPVGPLFKSSQAKSAAIRGDFMKSDDCIEWLNSKPQSSVVYVSFGSIVYLPQEQVDEIA 913
Query: 74 LGLELSNQKFLWV 86
GL+ S FLWV
Sbjct: 914 FGLKKSQVSFLWV 952
>TC17647 weakly similar to UP|AAR06918 (AAR06918) UDP-glycosyltransferase
91D1, partial (13%)
Length = 1059
Score = 47.0 bits (110), Expect = 6e-07
Identities = 25/72 (34%), Positives = 40/72 (54%), Gaps = 5/72 (6%)
Frame = -2
Query: 19 VYPVGPIIDTVTCSDRDA-----NGLECLSWLDKQQSCSVLYVSFGSGGTLSHEQIVQLA 73
V PVG + ++ D D + +E +WLD Q+ +V+Y+ FGS LS + + +LA
Sbjct: 218 VVPVGLLPPSMQIRDDDEEEKNPDWVEIKAWLDTQEPSTVVYIGFGSELKLSQQDLTELA 39
Query: 74 LGLELSNQKFLW 85
G++LS F W
Sbjct: 38 HGIKLSGLPFFW 3
>TC17030 similar to UP|Q8S995 (Q8S995) Glucosyltransferase-14, partial (51%)
Length = 1098
Score = 45.4 bits (106), Expect = 2e-06
Identities = 25/67 (37%), Positives = 38/67 (56%), Gaps = 1/67 (1%)
Frame = +1
Query: 16 NPSVYPVGPI-IDTVTCSDRDANGLECLSWLDKQQSCSVLYVSFGSGGTLSHEQIVQLAL 74
N V+ +GP+ + D + C+ WLD Q+S SV+Y GS L+ Q+++L L
Sbjct: 898 NGKVWCIGPVSLSNNNKVSIDEDEHYCMKWLDLQKSKSVIYACLGSMCNLTPLQLIELGL 1077
Query: 75 GLELSNQ 81
GLE SN+
Sbjct: 1078GLEASNR 1098
>AV427981
Length = 312
Score = 43.1 bits (100), Expect = 9e-06
Identities = 32/92 (34%), Positives = 47/92 (50%), Gaps = 8/92 (8%)
Frame = +3
Query: 1 MEMGPIKAPTEEGSGN--PSVYPVGPII-DTVTCS----DRDANGLECLSWLDKQQSCSV 53
+E+ + P +GS P P+ PI D +T S + D L CL WL Q+ SV
Sbjct: 45 LELQSHELPNNKGSQGVCPIALPIAPICRDQITKSLSFWEED---LSCLKWLATQKDNSV 215
Query: 54 LYVSFGSG-GTLSHEQIVQLALGLELSNQKFL 84
+Y+SFGS + ++ LAL LE S + F+
Sbjct: 216 VYISFGSWVSPIGEPKMKSLALALEASGRPFI 311
>TC9717 weakly similar to UP|Q9LNI1 (Q9LNI1) F6F3.22 protein, partial
(18%)
Length = 902
Score = 39.7 bits (91), Expect = 1e-04
Identities = 20/32 (62%), Positives = 24/32 (74%)
Frame = +1
Query: 52 SVLYVSFGSGGTLSHEQIVQLALGLELSNQKF 83
SV+YV+FGS TLS+E +LALGLELS F
Sbjct: 4 SVIYVAFGSEVTLSNEDFTELALGLELSGFPF 99
>TC9718 weakly similar to UP|Q9FI96 (Q9FI96) Glucuronosyl transferase-like
protein, partial (6%)
Length = 485
Score = 39.7 bits (91), Expect = 1e-04
Identities = 22/56 (39%), Positives = 34/56 (60%), Gaps = 2/56 (3%)
Frame = -2
Query: 19 VYPVGPIIDTVTCSDR--DANGLECLSWLDKQQSCSVLYVSFGSGGTLSHEQIVQL 72
V P G + ++ S+ D N ++WLDK + SV+YV+FGS TLS+E+ +L
Sbjct: 169 VIPFGLLPPSLEFSEESTDDNWDTIVNWLDKHEKGSVIYVAFGSEVTLSNEEFTEL 2
>BI421006
Length = 519
Score = 34.3 bits (77), Expect = 0.004
Identities = 19/36 (52%), Positives = 22/36 (60%)
Frame = +2
Query: 51 CSVLYVSFGSGGTLSHEQIVQLALGLELSNQKFLWV 86
CS+ F S T EQI Q+A GLE S QKF+WV
Sbjct: 86 CSICIFWFFS--TFIEEQIEQIANGLEQSKQKFIWV 187
Score = 30.8 bits (68), Expect = 0.045
Identities = 12/18 (66%), Positives = 15/18 (82%)
Frame = +3
Query: 42 LSWLDKQQSCSVLYVSFG 59
+ WLD+Q+ SVLYVSFG
Sbjct: 54 MEWLDRQEQKSVLYVSFG 107
>AV768184
Length = 417
Score = 32.0 bits (71), Expect = 0.020
Identities = 13/24 (54%), Positives = 18/24 (74%)
Frame = +1
Query: 42 LSWLDKQQSCSVLYVSFGSGGTLS 65
+ WLD+Q+ SV+YVSFGS T +
Sbjct: 346 MEWLDRQEPKSVIYVSFGSTTTFT 417
>TC16492 weakly similar to UP|AAR06919 (AAR06919) UDP-glycosyltransferase
88B1, partial (8%)
Length = 750
Score = 31.2 bits (69), Expect = 0.034
Identities = 13/31 (41%), Positives = 19/31 (60%)
Frame = +1
Query: 55 YVSFGSGGTLSHEQIVQLALGLELSNQKFLW 85
++ FGS LS + + +LA G+ELS F W
Sbjct: 1 FIGFGSELKLSQQDLTELAHGIELSGLPFFW 93
>TC16452 similar to UP|Q8LPJ3 (Q8LPJ3) Alpha-mannosidase, partial (9%)
Length = 641
Score = 27.7 bits (60), Expect = 0.38
Identities = 14/45 (31%), Positives = 20/45 (44%)
Frame = -3
Query: 1 MEMGPIKAPTEEGSGNPSVYPVGPIIDTVTCSDRDANGLECLSWL 45
+ MG A + GS P + G +D +TC + LSWL
Sbjct: 408 ISMGASSATSLSGSTGPPLETWGSGVDPLTCQTNRFLSISALSWL 274
>BP060297
Length = 364
Score = 26.9 bits (58), Expect = 0.64
Identities = 11/24 (45%), Positives = 17/24 (70%)
Frame = +2
Query: 42 LSWLDKQQSCSVLYVSFGSGGTLS 65
++WL+ + SV+YV FGS G L+
Sbjct: 293 MAWLNDRPKGSVVYVCFGSYGALN 364
>TC17057 similar to UP|VATD_ARATH (Q9XGM1) Vacuolar ATP synthase subunit D
(V-ATPase D subunit) (Vacuolar proton pump D subunit) ,
partial (59%)
Length = 561
Score = 26.6 bits (57), Expect = 0.84
Identities = 12/40 (30%), Positives = 16/40 (40%)
Frame = -3
Query: 5 PIKAPTEEGSGNPSVYPVGPIIDTVTCSDRDANGLECLSW 44
P+ APT PS+ VG C D + + SW
Sbjct: 163 PLVAPTRRAFTTPSIVTVGTTFRRCVCPDMVSRSVR*CSW 44
>TC16717 weakly similar to UP|Q41419 (Q41419) Sucrolytic enzyme/ferredoxin
homolog protein, partial (34%)
Length = 435
Score = 25.4 bits (54), Expect = 1.9
Identities = 12/32 (37%), Positives = 18/32 (55%)
Frame = -2
Query: 39 LECLSWLDKQQSCSVLYVSFGSGGTLSHEQIV 70
L CL+ + + C V++ SF GG E+IV
Sbjct: 287 LTCLTHRNFRLECDVVFASFKRGGDELRERIV 192
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.315 0.135 0.412
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,766,924
Number of Sequences: 28460
Number of extensions: 24260
Number of successful extensions: 87
Number of sequences better than 10.0: 54
Number of HSP's better than 10.0 without gapping: 86
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 86
length of query: 86
length of database: 4,897,600
effective HSP length: 62
effective length of query: 24
effective length of database: 3,133,080
effective search space: 75193920
effective search space used: 75193920
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 48 (23.1 bits)
Medicago: description of AC124966.9