BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC124966.6 + phase: 0
(176 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC8661 weakly similar to UP|O81348 (O81348) Symbiotic ammonium t... 45 9e-06
BP034811 39 5e-04
TC18919 similar to GB|AAN73298.1|25141207|BT002301 At4g16430/dl4... 37 0.001
TC9411 similar to UP|Q41102 (Q41102) Phaseolin G-box binding pro... 36 0.004
TC8348 similar to UP|Q94AC2 (Q94AC2) AT3g54020/F5K20_320, partia... 35 0.007
TC14843 similar to UP|Q9ZV88 (Q9ZV88) F9K20.26 protein (At1g7870... 32 0.080
CB828026 29 0.40
TC17329 weakly similar to UP|Q8H594 (Q8H594) OJ1634_B10.23 prote... 28 0.89
TC17162 similar to UP|Q8H5F1 (Q8H5F1) OJ1165_F02.16 protein, par... 27 1.5
TC10018 weakly similar to UP|HQGT_RAUSE (Q9AR73) Hydroquinone gl... 27 2.0
BP043127 27 2.0
AW163881 27 2.0
TC7815 similar to UP|Q9AVB5 (Q9AVB5) Plasma membrane intrinsic p... 26 3.4
TC12621 26 3.4
TC16664 similar to UP|BAD09830 (BAD09830) DNA-binding protein-li... 26 3.4
AV772495 25 5.8
TC7816 homologue to UP|Q9ATM4 (Q9ATM4) Plasma membrane integral ... 25 5.8
TC8779 similar to UP|Q8L934 (Q8L934) Nucleoid DNA-binding-like p... 25 5.8
TC15357 similar to UP|Q9ZV06 (Q9ZV06) At2g39020 protein, partial... 25 7.5
TC16879 similar to UP|Q9LRH6 (Q9LRH6) ZIM, partial (17%) 25 7.5
>TC8661 weakly similar to UP|O81348 (O81348) Symbiotic ammonium
transporter, partial (34%)
Length = 1007
Score = 44.7 bits (104), Expect = 9e-06
Identities = 20/31 (64%), Positives = 27/31 (86%)
Frame = +1
Query: 133 QDHIMAERKRRQVLTERFIALSATIPGLKKV 163
QDHI+AER+RR+ L++ IAL+A IPGLKK+
Sbjct: 25 QDHIIAERRRREKLSQSLIALAALIPGLKKM 117
>BP034811
Length = 578
Score = 38.9 bits (89), Expect = 5e-04
Identities = 39/135 (28%), Positives = 58/135 (42%), Gaps = 10/135 (7%)
Frame = +3
Query: 23 QPSFSFESGNSQSSTIQYMNNIVDNVCVTNTVH---ENIAQEYTWATNSQQLPPSQYILS 79
Q SF+ +S +S S+T + ++N+ +N E T YILS
Sbjct: 180 QRSFTIDSNHSHSTTTSTFKTEHCSSTMSNSSSGEDDNTTSERPSKTLKMSNASPSYILS 359
Query: 80 FENSTMQPSPNSDIATCSSIMVQETTTLNNNVSSELPKIIKKRTKN-------LRSSCEM 132
F N N D +T T +N++ + L KK TK RS +
Sbjct: 360 FSNENPAHILNFD-STLKPKSKALTVNVNHHGNRSLESQ-KKETKRKVQEGEKARSPHQA 533
Query: 133 QDHIMAERKRRQVLT 147
QDHI+AERKRR+ ++
Sbjct: 534 QDHIIAERKRREKIS 578
>TC18919 similar to GB|AAN73298.1|25141207|BT002301 At4g16430/dl4240w
{Arabidopsis thaliana;}, partial (29%)
Length = 604
Score = 37.4 bits (85), Expect = 0.001
Identities = 33/134 (24%), Positives = 61/134 (44%), Gaps = 3/134 (2%)
Frame = +3
Query: 33 SQSSTIQYMNNIVDNVCVTNTVHENIAQEYTWATNSQQ---LPPSQYILSFENSTMQPSP 89
SQS TI + + D+ T+ +E A A + + + + + + M P
Sbjct: 105 SQSITISFSPKVEDDSGFTSDSYEVQALGVNHAYGNSSNGCIGDGEAKMFPQLNQMIPGN 284
Query: 90 NSDIATCSSIMVQETTTLNNNVSSELPKIIKKRTKNLRSSCEMQDHIMAERKRRQVLTER 149
+ A SS+ + + ++ P+ ++ N R E +H+ AER+RR+ L +R
Sbjct: 285 FNPQARVSSLDLGNEDASSPHLDDRKPRKRGRKPANGRE--EPLNHVEAERQRREKLNQR 458
Query: 150 FIALSATIPGLKKV 163
F AL A +P + K+
Sbjct: 459 FYALRAVVPNISKM 500
>TC9411 similar to UP|Q41102 (Q41102) Phaseolin G-box binding protein PG2
(Fragment), partial (30%)
Length = 705
Score = 35.8 bits (81), Expect = 0.004
Identities = 17/44 (38%), Positives = 26/44 (58%)
Frame = +2
Query: 120 KKRTKNLRSSCEMQDHIMAERKRRQVLTERFIALSATIPGLKKV 163
K+ K E +H+ AER+RR+ L +RF AL A +P + K+
Sbjct: 146 KRGRKPANGREEPLNHVEAERQRREKLNQRFYALRAVVPNVSKM 277
>TC8348 similar to UP|Q94AC2 (Q94AC2) AT3g54020/F5K20_320, partial (37%)
Length = 776
Score = 35.0 bits (79), Expect = 0.007
Identities = 21/84 (25%), Positives = 38/84 (45%), Gaps = 2/84 (2%)
Frame = -2
Query: 19 IVQHQPSFSFESGNSQSSTIQY--MNNIVDNVCVTNTVHENIAQEYTWATNSQQLPPSQY 76
++Q +P+ F S +Q N D +C +I + ++W + LP S +
Sbjct: 631 LIQIRPTKYFHLTKSSD*RLQLPKFNIAADPICAAEA---HIPRHHSWQSQHGHLPESYH 461
Query: 77 ILSFENSTMQPSPNSDIATCSSIM 100
+ +F S +Q NS TC S++
Sbjct: 460 LFAFSVSNLQGQWNSHSLTCGSLL 389
>TC14843 similar to UP|Q9ZV88 (Q9ZV88) F9K20.26 protein
(At1g78700/F9K20_26), partial (23%)
Length = 598
Score = 31.6 bits (70), Expect = 0.080
Identities = 12/47 (25%), Positives = 23/47 (48%)
Frame = -2
Query: 50 VTNTVHENIAQEYTWATNSQQLPPSQYILSFENSTMQPSPNSDIATC 96
+ T Q +TW+ NSQ+ P+ I + S++ P + ++ C
Sbjct: 306 IEGTTDYQFMQHFTWSLNSQE*APNHLIRNLHGSSLLPKASPNLLCC 166
>CB828026
Length = 542
Score = 29.3 bits (64), Expect = 0.40
Identities = 13/30 (43%), Positives = 19/30 (63%)
Frame = +2
Query: 135 HIMAERKRRQVLTERFIALSATIPGLKKVS 164
H +AER RR+ ++ER L +PG KV+
Sbjct: 149 HSLAERVRREKISERMKFLQDLVPGCSKVT 238
>TC17329 weakly similar to UP|Q8H594 (Q8H594) OJ1634_B10.23 protein, partial
(24%)
Length = 486
Score = 28.1 bits (61), Expect = 0.89
Identities = 27/121 (22%), Positives = 52/121 (42%), Gaps = 8/121 (6%)
Frame = -1
Query: 43 NIVDNVCVTNTVHENIAQEYTWATNSQQLPPSQYI----LSFENSTMQPSPNSDIATCSS 98
NI+ ++ + H N + T N QQ PS ++ SF +T + + + S+
Sbjct: 483 NIIQHLSIPFPNHHNTQSKKTEKKNHQQH*PSHHVEKGLSSFRGAT------TLVYSISN 322
Query: 99 IMVQETTTLNNNVSSEL----PKIIKKRTKNLRSSCEMQDHIMAERKRRQVLTERFIALS 154
++ E N SS++ P I K + L S+ + + +R ++ IA++
Sbjct: 321 VLSVEIPLARRNCSSDIRTNSPSIKKSKGSGL*SASLFRRRALTMEERPSLVRSAGIAMA 142
Query: 155 A 155
A
Sbjct: 141 A 139
>TC17162 similar to UP|Q8H5F1 (Q8H5F1) OJ1165_F02.16 protein, partial (40%)
Length = 784
Score = 27.3 bits (59), Expect = 1.5
Identities = 25/107 (23%), Positives = 48/107 (44%), Gaps = 5/107 (4%)
Frame = +3
Query: 64 WATNSQQLPPSQYILSFENSTMQ-PSPNSDIATCSSIMVQET----TTLNNNVSSELPKI 118
W +N+ Q Y L N+ + P P + I + ++ T T + + + K+
Sbjct: 201 WRSNADQ---QIYSLRLANALRRIPRPAAQIRDTADRVLAATAKGRTRWSRAILTRRKKL 371
Query: 119 IKKRTKNLRSSCEMQDHIMAERKRRQVLTERFIALSATIPGLKKVSF 165
K+ K R++ ++ + RKR + ++ L +PG +KVSF
Sbjct: 372 RIKKVK--RAASGLRRAAIGRRKRLPAVQKKARVLGRLVPGCRKVSF 506
>TC10018 weakly similar to UP|HQGT_RAUSE (Q9AR73) Hydroquinone
glucosyltransferase (Arbutin synthase) , partial (24%)
Length = 586
Score = 26.9 bits (58), Expect = 2.0
Identities = 15/42 (35%), Positives = 21/42 (49%)
Frame = -1
Query: 2 GYDKSHFYLDEDKFQREIVQHQPSFSFESGNSQSSTIQYMNN 43
GY K+HF+L+ + HQP SGN SS + +N
Sbjct: 313 GYLKTHFHLE--------I*HQPPSYSSSGNEASSLLHLPSN 212
>BP043127
Length = 466
Score = 26.9 bits (58), Expect = 2.0
Identities = 11/30 (36%), Positives = 17/30 (56%)
Frame = -2
Query: 72 PPSQYILSFENSTMQPSPNSDIATCSSIMV 101
PPS +++ + SPN+D+ CS I V
Sbjct: 345 PPSLHVM*YLGMEFGESPNADVLACSLITV 256
>AW163881
Length = 236
Score = 26.9 bits (58), Expect = 2.0
Identities = 11/36 (30%), Positives = 19/36 (52%)
Frame = -1
Query: 65 ATNSQQLPPSQYILSFENSTMQPSPNSDIATCSSIM 100
+ N Q+ PSQ + + PN+D + CS+I+
Sbjct: 152 SANISQVQPSQRFQAIQRQFQHLQPNTDYSPCSAII 45
>TC7815 similar to UP|Q9AVB5 (Q9AVB5) Plasma membrane intrinsic protein
2-1, complete
Length = 1450
Score = 26.2 bits (56), Expect = 3.4
Identities = 24/97 (24%), Positives = 41/97 (41%), Gaps = 2/97 (2%)
Frame = -2
Query: 24 PSFSFESGNSQSSTIQYMNNIVDNVCVTNTVHENIAQEYTWATNSQQLPP--SQYILSFE 81
P F F +S S T ++N+ N + HE+ W+ N P S I E
Sbjct: 786 PDFVFVVDDSSSKTPCWVNSSTSNRDCSQVNHEHSKSNRKWSQNRNMGIPSVSLRISGRE 607
Query: 82 NSTMQPSPNSDIATCSSIMVQETTTLNNNVSSELPKI 118
NS + ++++T S + T L+ S+ +P +
Sbjct: 606 NSVDKNEGANNLSTQSGAVA--VTRLHKIGSATVPVV 502
>TC12621
Length = 897
Score = 26.2 bits (56), Expect = 3.4
Identities = 18/64 (28%), Positives = 30/64 (46%)
Frame = +1
Query: 54 VHENIAQEYTWATNSQQLPPSQYILSFENSTMQPSPNSDIATCSSIMVQETTTLNNNVSS 113
V+ N+ Q W + Q P+ Y +S + PN++ T SS + LNN+V
Sbjct: 205 VNPNVEQNDEWPSIEHQ--PAVY----GSSVVDTHPNTEYYTNSSNFGEANRQLNNHVDE 366
Query: 114 ELPK 117
+P+
Sbjct: 367 FVPE 378
>TC16664 similar to UP|BAD09830 (BAD09830) DNA-binding protein-like, partial
(22%)
Length = 1067
Score = 26.2 bits (56), Expect = 3.4
Identities = 15/51 (29%), Positives = 26/51 (50%), Gaps = 1/51 (1%)
Frame = +1
Query: 114 ELPKIIKKRTKNLRSSCE-MQDHIMAERKRRQVLTERFIALSATIPGLKKV 163
E P ++++ + S E +++H AER+RR + L IPG K+
Sbjct: 226 EAPMQLERKGVSPERSIEALRNHSEAERRRRARINAHLDTLRTVIPGANKM 378
>AV772495
Length = 572
Score = 25.4 bits (54), Expect = 5.8
Identities = 11/29 (37%), Positives = 16/29 (54%)
Frame = -3
Query: 135 HIMAERKRRQVLTERFIALSATIPGLKKV 163
H +AER RR+ + R L +PG K+
Sbjct: 423 HSLAERARREKINARMKLLQELVPGCYKL 337
>TC7816 homologue to UP|Q9ATM4 (Q9ATM4) Plasma membrane integral protein
ZmPIP2-7, complete
Length = 1523
Score = 25.4 bits (54), Expect = 5.8
Identities = 21/76 (27%), Positives = 31/76 (40%), Gaps = 2/76 (2%)
Frame = -3
Query: 24 PSFSFESGNSQSSTIQYMNNIVDNVCVTNTVHENIAQEYTWATNSQQLPPS--QYILSFE 81
P+F NS S T ++N N + HE+ + W PS I S E
Sbjct: 912 PNFVLVEDNSWSKTPCWVNASASNRDCSQVNHEHSKSNWKWCQYRNMGIPSVPLRISSRE 733
Query: 82 NSTMQPSPNSDIATCS 97
NS + + D++T S
Sbjct: 732 NSVDKNKGSYDLSTQS 685
>TC8779 similar to UP|Q8L934 (Q8L934) Nucleoid DNA-binding-like protein,
partial (27%)
Length = 737
Score = 25.4 bits (54), Expect = 5.8
Identities = 22/66 (33%), Positives = 31/66 (46%), Gaps = 1/66 (1%)
Frame = +3
Query: 63 TWATNSQQLPPSQYILSFENSTMQPSPNSDI-ATCSSIMVQETTTLNNNVSSELPKIIKK 121
T T S +PP ++ S N T+ P+ N I +T SI NNV+S L I
Sbjct: 120 TCYTGSIVVPPITFMFSGMNVTL-PTDNIVIHSTAGSITCLAMAVTPNNVNSVLNVIANM 296
Query: 122 RTKNLR 127
+ +N R
Sbjct: 297 QQQNHR 314
>TC15357 similar to UP|Q9ZV06 (Q9ZV06) At2g39020 protein, partial (69%)
Length = 1578
Score = 25.0 bits (53), Expect = 7.5
Identities = 19/76 (25%), Positives = 28/76 (36%)
Frame = +1
Query: 72 PPSQYILSFENSTMQPSPNSDIATCSSIMVQETTTLNNNVSSELPKIIKKRTKNLRSSCE 131
PP + + ST PS +S S TTTL+ T R C
Sbjct: 565 PPRSSLRTTNPSTPSPSSSSKSPRTPSPTPTSTTTLS--------------TSP*RKPCT 702
Query: 132 MQDHIMAERKRRQVLT 147
H M +R++R ++
Sbjct: 703 WSSHSMTQRRKRSEIS 750
>TC16879 similar to UP|Q9LRH6 (Q9LRH6) ZIM, partial (17%)
Length = 622
Score = 25.0 bits (53), Expect = 7.5
Identities = 11/23 (47%), Positives = 12/23 (51%)
Frame = -2
Query: 67 NSQQLPPSQYILSFENSTMQPSP 89
NS PP Y LS E S+ P P
Sbjct: 360 NSWPTPPEPYALSVETSSTPPPP 292
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.314 0.127 0.354
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,794,904
Number of Sequences: 28460
Number of extensions: 34059
Number of successful extensions: 229
Number of sequences better than 10.0: 44
Number of HSP's better than 10.0 without gapping: 229
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 229
length of query: 176
length of database: 4,897,600
effective HSP length: 84
effective length of query: 92
effective length of database: 2,506,960
effective search space: 230640320
effective search space used: 230640320
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 52 (24.6 bits)
Medicago: description of AC124966.6