BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC124963.7 + phase: 0
(512 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC11753 weakly similar to AAS55571 (AAS55571) At3g09690, partial... 111 3e-25
TC19315 similar to AAS49080 (AAS49080) At3g44520, partial (94%) 109 9e-25
TC15599 similar to UP|Q84VY8 (Q84VY8) At2g36290, partial (36%) 102 1e-22
BP080689 82 2e-16
TC11352 66 2e-11
TC9176 43 1e-04
TC14516 similar to UP|Q84VY8 (Q84VY8) At2g36290, partial (15%) 42 2e-04
TC14515 similar to UP|Q84VY8 (Q84VY8) At2g36290, partial (19%) 42 2e-04
TC18837 weakly similar to UP|Q39856 (Q39856) Epoxide hydrolase ... 38 0.003
BP067888 37 0.010
AV406816 31 0.55
TC14474 weakly similar to GB|AAA74598.1|598099|CSSPRP protamine ... 28 3.6
TC13840 similar to GB|AAM78055.1|21928053|AY125545 At4g29227 {Ar... 28 3.6
AV778988 27 6.1
TC10423 27 8.0
>TC11753 weakly similar to AAS55571 (AAS55571) At3g09690, partial (9%)
Length = 630
Score = 111 bits (277), Expect = 3e-25
Identities = 49/61 (80%), Positives = 55/61 (89%)
Frame = +3
Query: 445 YIERVLPEAVIHKLPNEGHFSYFFFCDECHKQIFSTLFGTPQGPFERQEETMLEKNTEDA 504
YIERVLPEAVIHKLPNEGHFSYFFFC++CH+QIF+TLFG PQGP ERQEET LE+ E+
Sbjct: 3 YIERVLPEAVIHKLPNEGHFSYFFFCEKCHRQIFTTLFGIPQGPVERQEETALEETKEEV 182
Query: 505 L 505
L
Sbjct: 183 L 185
>TC19315 similar to AAS49080 (AAS49080) At3g44520, partial (94%)
Length = 594
Score = 109 bits (273), Expect = 9e-25
Identities = 51/133 (38%), Positives = 86/133 (64%)
Frame = +1
Query: 151 PPIASRILLPDGRYMAYQDQGVPPGRARFSLVAPHSFLSSRLAGIPGVKASLLEDYGVRL 210
P ++ RI L DGR++AY ++GVP A++ ++ H F SS+ + L+++ G+ L
Sbjct: 187 PVVSPRIRLRDGRHLAYVERGVPKDMAKYKIIIVHGFGSSKEMNFLAPQ-ELIDELGIYL 363
Query: 211 VTYDLPGFGESDPHPSRNFNSSAMDMLHLVDAVNVTDKFWVLCHSSGCIHAWASLKYIPE 270
+ YD G+GESDP+P R+ S A+D+ L D + V +F+V+ S G W+ LKY+P+
Sbjct: 364 LQYDRAGYGESDPNPKRSLKSEALDIQELADQLEVGAEFYVIGVSMGSYATWSCLKYLPD 543
Query: 271 RIAGAAMLAPMVS 283
R+AG A++AP+++
Sbjct: 544 RLAGLALIAPVIN 582
>TC15599 similar to UP|Q84VY8 (Q84VY8) At2g36290, partial (36%)
Length = 698
Score = 102 bits (255), Expect = 1e-22
Identities = 57/136 (41%), Positives = 82/136 (59%), Gaps = 3/136 (2%)
Frame = +2
Query: 151 PPI-ASRILLPDGRYMAYQDQGVPPGRARFSLVAPHSFLSSRLAGIPGVKAS--LLEDYG 207
PP+ A RI L DGR++AY++ GVP A++ ++ H SS A S + ED G
Sbjct: 287 PPVTAPRIKLRDGRHLAYKEHGVPKDTAKYKIIFVHGLGSSIHASPVAETLSPHVAEDLG 466
Query: 208 VRLVTYDLPGFGESDPHPSRNFNSSAMDMLHLVDAVNVTDKFWVLCHSSGCIHAWASLKY 267
+ +V++D PG+GESDP P+R S A D+ L D + + KF++ S G W+ LKY
Sbjct: 467 IYIVSFDRPGYGESDPDPNRTPKSIAFDIEDLADKLELGSKFFLTGLSMGGQIVWSCLKY 646
Query: 268 IPERIAGAAMLAPMVS 283
IP R+AGAA+L P V+
Sbjct: 647 IPHRLAGAALLTPAVN 694
>BP080689
Length = 326
Score = 82.0 bits (201), Expect = 2e-16
Identities = 41/87 (47%), Positives = 54/87 (61%)
Frame = +2
Query: 1 MAEARGTTWWSEELASLMENSLPESSTTTSFEDVRKSSSSEEVEVGESLKEHAVGFLMAW 60
M E +GT WW +ELASL+++S E ESLKE VGF+MAW
Sbjct: 107 MVEVKGTNWWRDELASLVDDSAARYDDGDPPE--------------ESLKEQFVGFVMAW 244
Query: 61 CEILMELGRGCRDILQQNFFNEDSYLV 87
CEIL+EL RGCRDI++QN +E+S++V
Sbjct: 245 CEILLELARGCRDIVRQNLLSEESFVV 325
>TC11352
Length = 738
Score = 65.9 bits (159), Expect = 2e-11
Identities = 53/202 (26%), Positives = 90/202 (44%), Gaps = 3/202 (1%)
Frame = +1
Query: 265 LKYIPERIAGAAMLAPMVSPYESHMTKDEMKRTWEKWLPRRKYMYSLAYRFPKLLSFFYR 324
L YIP R+AG AM+AP+++ + + +K + + L K+ LA PKLL ++
Sbjct: 22 LHYIPNRLAGVAMIAPIINYKWPSLPESLVKDDYRRKLV--KFSLWLAIYTPKLLHWWVS 195
Query: 325 KSFLPEKHERIDKQFSLSLGKKDEILVDEPAFEEYWQRDLEESVRQGNLKPFIEEALLQV 384
+ +LP + I+K + G+ +IL P F + L + V L+ + L+
Sbjct: 196 QKWLP-SNSVIEKNPAFFNGRDIDILKRIPGFPMLTKDKLRDQVVFDTLR---GDWLVAF 363
Query: 385 SRWDFNIEEL---HVHKKCQTGGLLLWLKSMYGQAECELAGYLGRIHIWQGLDDRMVPPS 441
W+F+ +L H K +HIWQG +D++VP
Sbjct: 364 GNWEFDPMKLSSPFPHNK-------------------------SSVHIWQGYEDKVVPSK 468
Query: 442 MTEYIERVLPEAVIHKLPNEGH 463
+ Y+ LP H++P+ GH
Sbjct: 469 IQRYVSEKLPWIQYHEVPDGGH 534
>TC9176
Length = 671
Score = 42.7 bits (99), Expect = 1e-04
Identities = 26/86 (30%), Positives = 46/86 (53%), Gaps = 4/86 (4%)
Frame = +1
Query: 203 LEDYGVRLVTYDLPGFGESD-PHPSRNFNSSAM--DMLHLVDAVNVT-DKFWVLCHSSGC 258
L G R V DL G+G++D P ++ S + D++ L+D + +K +V+ H G
Sbjct: 190 LASLGYRCVAPDLRGYGDTDVPAAQTSYTSLHVVGDLIRLLDVIAPDQEKVFVVGHDWGA 369
Query: 259 IHAWASLKYIPERIAGAAMLAPMVSP 284
+ AW+ +Y PER+ +L+ +P
Sbjct: 370 LTAWSLSQYRPERVKAMVILSVAFTP 447
>TC14516 similar to UP|Q84VY8 (Q84VY8) At2g36290, partial (15%)
Length = 442
Score = 42.4 bits (98), Expect = 2e-04
Identities = 21/59 (35%), Positives = 31/59 (51%)
Frame = +2
Query: 425 GRIHIWQGLDDRMVPPSMTEYIERVLPEAVIHKLPNEGHFSYFFFCDECHKQIFSTLFG 483
G +H+WQG +D +VP ++ +I + LP H+LP GH F D I +L G
Sbjct: 56 GSVHLWQGDEDLLVPVTVQRHIAQNLPWIQYHELPGSGHL--FCLGDGISDTIIKSLLG 226
>TC14515 similar to UP|Q84VY8 (Q84VY8) At2g36290, partial (19%)
Length = 635
Score = 42.0 bits (97), Expect = 2e-04
Identities = 21/59 (35%), Positives = 31/59 (51%)
Frame = +1
Query: 425 GRIHIWQGLDDRMVPPSMTEYIERVLPEAVIHKLPNEGHFSYFFFCDECHKQIFSTLFG 483
G +H+WQG +D +VP ++ +I + LP H+LP GH F D I +L G
Sbjct: 190 GSVHLWQGDEDLLVPVTLQRHIAQNLPWIQYHELPGSGHM--FCLGDGTSDIIIKSLLG 360
>TC18837 weakly similar to UP|Q39856 (Q39856) Epoxide hydrolase , partial
(89%)
Length = 1100
Score = 38.1 bits (87), Expect = 0.003
Identities = 25/86 (29%), Positives = 46/86 (53%), Gaps = 4/86 (4%)
Frame = +1
Query: 203 LEDYGVRLVTYDLPGFGESDPHPSRNFNSSAM----DMLHLVDAVNVTDKFWVLCHSSGC 258
L G R + DL G+G++D P+ + + SA+ D++ L+DA+ V D+ +++ H G
Sbjct: 130 LSALGYRAIAPDLRGYGDTDA-PTDSSSYSALHIVGDLVGLLDALAV-DRVFLVGHDWGA 303
Query: 259 IHAWASLKYIPERIAGAAMLAPMVSP 284
AW + PER+ ++ + P
Sbjct: 304 AMAWYFALFKPERVIALVNMSVVFRP 381
>BP067888
Length = 534
Score = 36.6 bits (83), Expect = 0.010
Identities = 26/99 (26%), Positives = 45/99 (45%), Gaps = 3/99 (3%)
Frame = +3
Query: 203 LEDYGVRLVTYDLPGFGESD-PHPSRNFNSSAM--DMLHLVDAVNVTDKFWVLCHSSGCI 259
L G R V DL G+G++D P + M D++ L+D++ V D+ +++ H G +
Sbjct: 192 LSALGYRAVAPDLRGYGDTDAPASVTRYACFHMVGDVVALIDSLGV-DQVFLVAHDWGAM 368
Query: 260 HAWASLKYIPERIAGAAMLAPMVSPYESHMTKDEMKRTW 298
W + PE+I L+ P + + R W
Sbjct: 369 MGWYLCWFRPEKIKAYVCLSVPFMPRNPKVKPVDAMRAW 485
>AV406816
Length = 417
Score = 30.8 bits (68), Expect = 0.55
Identities = 22/75 (29%), Positives = 38/75 (50%), Gaps = 3/75 (4%)
Frame = +1
Query: 203 LEDYGVRLVTYDLPGFGESDPHP---SRNFNSSAMDMLHLVDAVNVTDKFWVLCHSSGCI 259
L + G R + D G+G SDP P + F+ D+L ++DA+ ++ K +++ G
Sbjct: 172 LANAGFRAIAPDFRGYGLSDPPPEPENATFSVLLSDLLAILDALALS-KVFLVGKDFGSR 348
Query: 260 HAWASLKYIPERIAG 274
A+ PER+ G
Sbjct: 349 PAYLFSILHPERVLG 393
>TC14474 weakly similar to GB|AAA74598.1|598099|CSSPRP protamine P1
{Caenolestes fuliginosus;} , partial (49%)
Length = 1266
Score = 28.1 bits (61), Expect = 3.6
Identities = 17/44 (38%), Positives = 29/44 (65%), Gaps = 1/44 (2%)
Frame = -2
Query: 2 AEARGTTWWSEELASLMENSLPESSTTTSFEDVR-KSSSSEEVE 44
AEA G++ S++ + + +S +SST+ S E++ KSSS E+E
Sbjct: 437 AEAEGSSSNSDDESFPVSSSSSKSSTSPSSEEISLKSSSKREIE 306
>TC13840 similar to GB|AAM78055.1|21928053|AY125545 At4g29227 {Arabidopsis
thaliana;}, partial (12%)
Length = 528
Score = 28.1 bits (61), Expect = 3.6
Identities = 17/44 (38%), Positives = 21/44 (47%)
Frame = +3
Query: 395 HVHKKCQTGGLLLWLKSMYGQAECELAGYLGRIHIWQGLDDRMV 438
H K C+ LL W S E EL ++ RIH W L DR +
Sbjct: 45 HQRKCCRL--LLKWQLSSVELWELELVFHIVRIHTWHPLYDREI 170
>AV778988
Length = 531
Score = 27.3 bits (59), Expect = 6.1
Identities = 12/34 (35%), Positives = 17/34 (49%)
Frame = +3
Query: 392 EELHVHKKCQTGGLLLWLKSMYGQAECELAGYLG 425
EELH HK+C GG + G+ + + Y G
Sbjct: 129 EELHSHKECNPGGAFAHSLKV*GKCKRRVIAYSG 230
>TC10423
Length = 587
Score = 26.9 bits (58), Expect = 8.0
Identities = 12/34 (35%), Positives = 17/34 (49%)
Frame = -1
Query: 262 WASLKYIPERIAGAAMLAPMVSPYESHMTKDEMK 295
W S YI + +G + P V+ YE+ DE K
Sbjct: 395 WESYVYINKCFSGTGIYTPTVTSYETISVVDEFK 294
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.320 0.136 0.418
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,007,472
Number of Sequences: 28460
Number of extensions: 153253
Number of successful extensions: 789
Number of sequences better than 10.0: 30
Number of HSP's better than 10.0 without gapping: 784
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 786
length of query: 512
length of database: 4,897,600
effective HSP length: 94
effective length of query: 418
effective length of database: 2,222,360
effective search space: 928946480
effective search space used: 928946480
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 57 (26.6 bits)
Medicago: description of AC124963.7