BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC124963.24 - phase: 0
(556 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC12569 UP|Q40221 (Q40221) Protein containing C-terminal RING-fi... 861 0.0
TC15641 125 2e-29
TC8964 weakly similar to UP|Q94F54 (Q94F54) AT3g15070/K15M2_22, ... 117 5e-27
TC15152 similar to UP|Q8RZY3 (Q8RZY3) RING zinc finger protein-l... 110 5e-25
TC8730 similar to PIR|H96764|H96764 protein RING zinc finger pro... 107 7e-24
AV779072 105 1e-23
TC12534 similar to UP|Q9MAJ1 (Q9MAJ1) F27F5.26, partial (13%) 88 3e-18
TC17068 similar to UP|Q8L775 (Q8L775) RING finger-like protein, ... 77 1e-14
TC16177 similar to GB|AAO42794.1|28416527|BT004548 At5g42940/MBD... 59 3e-09
TC15079 similar to UP|Q84KA9 (Q84KA9) RING/C3HC4/PHD zinc finger... 58 4e-09
BP067396 58 5e-09
BP042964 57 8e-09
TC9362 similar to PIR|F86340|F86340 protein F2D10.34 [imported] ... 56 1e-08
TC19649 weakly similar to UP|RNF6_HUMAN (Q9Y252) RING finger pro... 55 2e-08
BP045442 55 2e-08
BP055954 55 2e-08
AU252073 55 3e-08
BP073862 55 4e-08
BP037094 53 1e-07
TC13875 52 2e-07
>TC12569 UP|Q40221 (Q40221) Protein containing C-terminal RING-finger,
complete
Length = 2092
Score = 861 bits (2225), Expect = 0.0
Identities = 422/557 (75%), Positives = 473/557 (84%), Gaps = 1/557 (0%)
Frame = +2
Query: 1 MGHRHLFNASPMFEGEADPNWNNMLTDQHHVNHGGTSSSENGSFIYPVENMSIDNIYFPS 60
MGHRHL+N +P+FEGE D NWN+M TDQH+VN G TS++ENGS+ YPVEN+SID+ FPS
Sbjct: 299 MGHRHLYNTTPLFEGEPDQNWNHMHTDQHYVNLGRTSTAENGSYFYPVENVSIDSTSFPS 478
Query: 61 HWNANTRSNGYASSGPNIEVPPPHQLDTSGTSTNDHFMHSSSAGPFFAVSENFVHQPSSS 120
HWN TRSNGYASS NIEVPP HQ DTSG S NDHF+HSSSAG FFAVS N V QP ++
Sbjct: 479 HWNPATRSNGYASSSLNIEVPP-HQSDTSGAS-NDHFIHSSSAGAFFAVSNNCVPQPPAA 652
Query: 121 NYDRQAFHVDSGFIDLTMGSGRAHHKRKSPGIPSVYERGSSSGYFNAGTSSDLPIPPESW 180
NYDR F VD GFIDLTMGSGR HKRKSPGIPSVYERG SS YFNAG+S+D+PI ES
Sbjct: 653 NYDRPPFQVDGGFIDLTMGSGRGPHKRKSPGIPSVYERGGSSRYFNAGSSTDVPISSESR 832
Query: 181 PEKPNMDPQYMPWDHAAMAPTFRGAGLSMKGESSVRNVRSRSSLDLESNLCRTHLSSSHS 240
PEKPN+D QYMPWDH M T RGAG S+KGESS+RNVRSRS+LDLESNL RTHL S+HS
Sbjct: 833 PEKPNIDSQYMPWDHVPMTSTVRGAGHSIKGESSLRNVRSRSALDLESNLSRTHLPSNHS 1012
Query: 241 HNSYPTVPPVSHSSLADLS-QVSTSLTRDWSQMNVTPANGRVLLPDASTFGLETSHFPVG 299
HNSY T+PPV HSS+ DLS Q+STSLT DWSQMN++PANGRVLLPDA +GLE+SHF G
Sbjct: 1013HNSYSTIPPVDHSSMMDLSGQISTSLTGDWSQMNISPANGRVLLPDAGAYGLESSHFLAG 1192
Query: 300 NAAAAAAASNATVDVGSFHHDFGTSRNPTTAQSFQNLTQTARGTRSNYSQRSTPAYRASS 359
+ A ASNA+VDVGSFHH+FGTSRNPT QSF NLTQTARG RSNYSQRS P +RASS
Sbjct: 1193---SGATASNASVDVGSFHHEFGTSRNPTGPQSFHNLTQTARGIRSNYSQRSAPTFRASS 1363
Query: 360 SLRLGQATPSDNGLPMVAEGYPSRHPRPLNTVGWRNSDRNGRSRISSERYRSLADQAALH 419
S LG TP D+GLPMVAE Y SRH RPL+++GWRNSDRNGR+RISSERYRSLA++A LH
Sbjct: 1364STHLGHVTPLDDGLPMVAESYSSRHQRPLSSIGWRNSDRNGRARISSERYRSLANEAGLH 1543
Query: 420 ARLSSSEVPGFMIVERASLYGSRNVLDQHREMRMDIDNMSYEELLALGERIGQVNTGLSE 479
R+S FMIV+RAS+YGSRN+LDQHR+MRMDIDNMSYEELLALGERIG VNTGLSE
Sbjct: 1544GRISPEV---FMIVDRASMYGSRNMLDQHRDMRMDIDNMSYEELLALGERIGHVNTGLSE 1714
Query: 480 DVLSKCVTETIYCSSDQCQDEGSCVICLEEYKNMDDVGTLKTCGHDYHVSCIKKWLSMKK 539
D S+C+TETIYCSS+Q QDEGSCVICLEEYKNMDDVGTLKTCGHDYHV+CIKKWLSMKK
Sbjct: 1715DSFSQCMTETIYCSSEQGQDEGSCVICLEEYKNMDDVGTLKTCGHDYHVNCIKKWLSMKK 1894
Query: 540 LCPICKSSALPEDKKDK 556
LCPICK+S +PEDK +K
Sbjct: 1895LCPICKASVMPEDKMNK 1945
>TC15641
Length = 525
Score = 125 bits (314), Expect = 2e-29
Identities = 59/113 (52%), Positives = 81/113 (71%), Gaps = 2/113 (1%)
Frame = +2
Query: 439 YGSRNVLDQHREMRMDIDNMSYEELLALGERIGQVNTGLSEDVLSKCVTETIYCS--SDQ 496
+G + D+HREMR+D+DNMSYEELLAL E+IG V+TGLSED + K + + +Y S ++
Sbjct: 5 HGLAEMHDRHREMRLDVDNMSYEELLALEEQIGDVSTGLSEDNIHKLMKQRLYISFMAES 184
Query: 497 CQDEGSCVICLEEYKNMDDVGTLKTCGHDYHVSCIKKWLSMKKLCPICKSSAL 549
D C IC E+Y N + +G+L CGH++H CIK+WL K LCPICK++AL
Sbjct: 185 SSDPEPCCICQEQYANGEIIGSL-DCGHEFHTKCIKQWLVQKNLCPICKTTAL 340
>TC8964 weakly similar to UP|Q94F54 (Q94F54) AT3g15070/K15M2_22, partial
(19%)
Length = 594
Score = 117 bits (293), Expect = 5e-27
Identities = 54/112 (48%), Positives = 78/112 (69%), Gaps = 10/112 (8%)
Frame = +1
Query: 448 HREMRMDIDNMSYEELLALGERIGQVNTGLSEDVLSKCVTETIYC----------SSDQC 497
HR+MR+DI++MSYE+LLALGERIG V+TGLSE+ +S + Y ++
Sbjct: 1 HRDMRLDIEDMSYEDLLALGERIGNVSTGLSEEAISNPLKTKTYLAHATSVNEEEAASND 180
Query: 498 QDEGSCVICLEEYKNMDDVGTLKTCGHDYHVSCIKKWLSMKKLCPICKSSAL 549
Q+ SC+IC +E+KN +++G L+ C H+YH C++KWL +K +CPICKS AL
Sbjct: 181 QETDSCMICQDEFKNQEEIGILR-CEHEYHAECLRKWLLVKNVCPICKSEAL 333
>TC15152 similar to UP|Q8RZY3 (Q8RZY3) RING zinc finger protein-like,
partial (11%)
Length = 779
Score = 110 bits (276), Expect = 5e-25
Identities = 49/103 (47%), Positives = 71/103 (68%)
Frame = +3
Query: 443 NVLDQHREMRMDIDNMSYEELLALGERIGQVNTGLSEDVLSKCVTETIYCSSDQCQDEGS 502
N D+HR++R+D+DNMSYE+LL L ERIG V+TGL E+ + K + + + S D Q + +
Sbjct: 159 NSQDRHRDLRLDVDNMSYEQLLELSERIGYVSTGLKEEEMRKNIRK-LQISDDASQVDKN 335
Query: 503 CVICLEEYKNMDDVGTLKTCGHDYHVSCIKKWLSMKKLCPICK 545
C IC EEY+ +++G L CGH YH CI++W+ K CP+CK
Sbjct: 336 CTICQEEYEVEEEIGRL-NCGHSYHYQCIQQWVEQKNFCPVCK 461
>TC8730 similar to PIR|H96764|H96764 protein RING zinc finger protein
F25P22.18 [imported] - Arabidopsis
thaliana {Arabidopsis thaliana;}, partial (23%)
Length = 583
Score = 107 bits (266), Expect = 7e-24
Identities = 53/113 (46%), Positives = 75/113 (65%), Gaps = 3/113 (2%)
Frame = +3
Query: 440 GSRNVLDQHREMRMDIDNMSYEELLALGERIGQVNTGLSEDVLSKCVTE-TIYCSSD--Q 496
G N DQ R+ R+DIDNMSYE+LL LGE+IG VNTG+ ED + + + ++ S+D +
Sbjct: 9 GRLNSHDQFRDWRLDIDNMSYEQLLELGEKIGYVNTGVKEDEMELNIRKLKLFISNDTSK 188
Query: 497 CQDEGSCVICLEEYKNMDDVGTLKTCGHDYHVSCIKKWLSMKKLCPICKSSAL 549
Q + C IC EEY++ D++G LK C H +H CIK+WL +K CP+CK +
Sbjct: 189 HQLDRKCTICQEEYESDDELGKLK-CEHCFHFQCIKQWLVLKNFCPVCKQEVV 344
>AV779072
Length = 531
Score = 105 bits (263), Expect = 1e-23
Identities = 52/98 (53%), Positives = 67/98 (68%), Gaps = 1/98 (1%)
Frame = -1
Query: 453 MDIDNMSYEELLALGERIGQVNTGLSEDVLSKCVTETIYCSSDQCQ-DEGSCVICLEEYK 511
+D+DNMSYEELLAL ERIG VNTGL+E+ + K + + Y + Q D C +C EEY
Sbjct: 531 LDVDNMSYEELLALEERIGNVNTGLTEETIMKLLIQKQYSAEPGSQGDAEPCSVCQEEYD 352
Query: 512 NMDDVGTLKTCGHDYHVSCIKKWLSMKKLCPICKSSAL 549
+ DD+G+L CGH YH CIK+WL K LCPI K++ L
Sbjct: 351 DGDDIGSL-YCGHFYHTGCIKQWLMHKNLCPIRKTTGL 241
>TC12534 similar to UP|Q9MAJ1 (Q9MAJ1) F27F5.26, partial (13%)
Length = 941
Score = 88.2 bits (217), Expect = 3e-18
Identities = 54/146 (36%), Positives = 78/146 (52%), Gaps = 1/146 (0%)
Frame = +2
Query: 400 GRSRISSERYRSLADQAALHARLSSSEVPGFMIVERASLYGSRNVLDQHREMRMDIDNMS 459
G +R+ SE L + L R + + MI++++ G N+ D+HR+MR+D+DNMS
Sbjct: 506 GGNRLVSE----LRNVLGLMRRGGNLRLEDVMILDQSMFAGIANIHDRHRDMRLDVDNMS 673
Query: 460 YEELLALGERIGQVNTGLSEDVLSKCVTETIYCSSDQCQD-EGSCVICLEEYKNMDDVGT 518
YEELLAL ERIG VNTGL+E+ + K + + Y + Q +C +C Y M +
Sbjct: 674 YEELLALEERIGNVNTGLTEETIMKLLKQXKYSAEPXSQG*CXTCCVCXXXY--MMXXXS 847
Query: 519 LKTCGHDYHVSCIKKWLSMKKLCPIC 544
YH K+WL L PIC
Sbjct: 848 DPQLRAFYHTGLFKQWLMHXXLXPIC 925
>TC17068 similar to UP|Q8L775 (Q8L775) RING finger-like protein, partial
(9%)
Length = 549
Score = 76.6 bits (187), Expect = 1e-14
Identities = 38/88 (43%), Positives = 56/88 (63%), Gaps = 7/88 (7%)
Frame = +2
Query: 466 LGERIGQVNTGLSEDVLSKCVTETIYCSS-------DQCQDEGSCVICLEEYKNMDDVGT 518
L ER+ V+T ++E+ LS+C+ + Y S ++ +D+ C IC EEY D+VG+
Sbjct: 2 LEERMATVSTAVTEEALSECLKKNPYQPSASDNVTCNENKDDIKCSICQEEYVAADEVGS 181
Query: 519 LKTCGHDYHVSCIKKWLSMKKLCPICKS 546
LK C H YHV CI++WL +K CPICK+
Sbjct: 182 LK-CEHKYHVVCIQQWLRLKNWCPICKA 262
>TC16177 similar to GB|AAO42794.1|28416527|BT004548 At5g42940/MBD2_14
{Arabidopsis thaliana;}, partial (5%)
Length = 530
Score = 58.5 bits (140), Expect = 3e-09
Identities = 24/41 (58%), Positives = 31/41 (75%)
Frame = +2
Query: 509 EYKNMDDVGTLKTCGHDYHVSCIKKWLSMKKLCPICKSSAL 549
+Y + DD+GTL CGHD+H CIK+WL K LCPICK++ L
Sbjct: 2 DYNDGDDIGTLD-CGHDFHSYCIKQWLMHKNLCPICKTTGL 121
>TC15079 similar to UP|Q84KA9 (Q84KA9) RING/C3HC4/PHD zinc finger-like
protein, partial (20%)
Length = 921
Score = 58.2 bits (139), Expect = 4e-09
Identities = 26/86 (30%), Positives = 48/86 (55%), Gaps = 4/86 (4%)
Frame = +3
Query: 469 RIGQVNTGLSEDVLSKCVTETIYCSSDQCQDEG----SCVICLEEYKNMDDVGTLKTCGH 524
++ +++ GL+++V+ T S +C G +C +CL E+++ + + + C H
Sbjct: 516 QLNELSNGLNQEVID--TFPTFLYSHVKCLKIGKGTLACAVCLNEFEDDETLRLIPICNH 689
Query: 525 DYHVSCIKKWLSMKKLCPICKSSALP 550
YH SCI WL+ CP+C++S LP
Sbjct: 690 VYHHSCIDLWLASHSTCPVCRASLLP 767
>BP067396
Length = 506
Score = 57.8 bits (138), Expect = 5e-09
Identities = 22/52 (42%), Positives = 30/52 (57%)
Frame = -1
Query: 503 CVICLEEYKNMDDVGTLKTCGHDYHVSCIKKWLSMKKLCPICKSSALPEDKK 554
C ICL +YK D + L CGH YH++C+ WL CPIC+ S++ K
Sbjct: 386 CSICLGDYKEADTLRMLPDCGHVYHLACVDPWLKFHSTCPICRKSSVQSSIK 231
>BP042964
Length = 575
Score = 57.0 bits (136), Expect = 8e-09
Identities = 28/98 (28%), Positives = 49/98 (49%), Gaps = 2/98 (2%)
Frame = +1
Query: 454 DIDNMSYEELLALGERIGQVNTGLSEDVLSKCVTETIYCSSDQCQDE--GSCVICLEEYK 511
D+D++ Y+ G + GL E V+ K + ++Y ++ C +CL +++
Sbjct: 163 DVDSLQYDSTTLDGPHLFSPY-GLDESVI-KTIPFSLYTPKLHIEESRRSDCAVCLLDFE 336
Query: 512 NMDDVGTLKTCGHDYHVSCIKKWLSMKKLCPICKSSAL 549
+ D V TL C H +H+ CI WL CP+C++ L
Sbjct: 337 HQDYVRTLPICSHTFHLDCIDAWLRSHANCPLCRAGVL 450
>TC9362 similar to PIR|F86340|F86340 protein F2D10.34 [imported] -
Arabidopsis thaliana {Arabidopsis thaliana;}, partial
(58%)
Length = 591
Score = 56.2 bits (134), Expect = 1e-08
Identities = 27/83 (32%), Positives = 39/83 (46%)
Frame = +1
Query: 474 NTGLSEDVLSKCVTETIYCSSDQCQDEGSCVICLEEYKNMDDVGTLKTCGHDYHVSCIKK 533
N G+ + VL T +++ C ICL E+ D++ L CGH +HVSCI
Sbjct: 247 NKGVKKKVLRSIPKLT--ATAESAVKFSDCAICLTEFAAGDEIRVLPQCGHGFHVSCIDA 420
Query: 534 WLSMKKLCPICKSSALPEDKKDK 556
WL CP C+ + + DK
Sbjct: 421 WLKSHSSCPSCRQILVAASRCDK 489
>TC19649 weakly similar to UP|RNF6_HUMAN (Q9Y252) RING finger protein 6
(RING-H2 protein), partial (4%)
Length = 523
Score = 55.5 bits (132), Expect = 2e-08
Identities = 21/49 (42%), Positives = 30/49 (60%)
Frame = +2
Query: 499 DEGSCVICLEEYKNMDDVGTLKTCGHDYHVSCIKKWLSMKKLCPICKSS 547
D+ +C ICL EY + V T+ C H +H CI +WL + CPIC++S
Sbjct: 146 DDKACPICLSEYMPKETVKTIPECEHCFHAQCIDEWLPLNASCPICRNS 292
>BP045442
Length = 488
Score = 55.5 bits (132), Expect = 2e-08
Identities = 23/62 (37%), Positives = 39/62 (62%), Gaps = 1/62 (1%)
Frame = -1
Query: 492 CSSDQCQDEGSCVICLEEYKNMDDVGTLKTCGHDYHVSCIKKWLSMKKL-CPICKSSALP 550
CSS + Q E C +CL +++ ++ L CGH +H +C++KWL + CP+C++ +P
Sbjct: 470 CSSCKQQPEHECSVCLTKFEPESEINCLP-CGHLFHKACLEKWLDYWNITCPLCRTPLMP 294
Query: 551 ED 552
ED
Sbjct: 293 ED 288
>BP055954
Length = 518
Score = 55.5 bits (132), Expect = 2e-08
Identities = 19/60 (31%), Positives = 36/60 (59%)
Frame = +1
Query: 491 YCSSDQCQDEGSCVICLEEYKNMDDVGTLKTCGHDYHVSCIKKWLSMKKLCPICKSSALP 550
Y ++++ E +C ICL E+K + + + C H +H+ C+ WL + CP+C++S +P
Sbjct: 265 YAAAERGHGETTCSICLCEFKEAEMLRMMPECRHHFHLCCLDSWLKLNGSCPVCRNSPMP 444
>AU252073
Length = 258
Score = 55.1 bits (131), Expect = 3e-08
Identities = 19/47 (40%), Positives = 30/47 (63%)
Frame = +1
Query: 499 DEGSCVICLEEYKNMDDVGTLKTCGHDYHVSCIKKWLSMKKLCPICK 545
++ CVICL +YK + + + CGH +H+SCI WL + CP+C+
Sbjct: 22 EDTQCVICLADYKEREVLRIMPKCGHTFHLSCIDIWLRKQSTCPVCR 162
>BP073862
Length = 348
Score = 54.7 bits (130), Expect = 4e-08
Identities = 24/43 (55%), Positives = 30/43 (68%)
Frame = -2
Query: 506 CLEEYKNMDDVGTLKTCGHDYHVSCIKKWLSMKKLCPICKSSA 548
C EEY D+VGTL+ C H +HV CI++WL +K CPICK A
Sbjct: 347 CQEEYIVADEVGTLQ-CEHIFHVVCIQEWLRLKNWCPICKEEA 222
>BP037094
Length = 502
Score = 53.1 bits (126), Expect = 1e-07
Identities = 24/55 (43%), Positives = 31/55 (55%), Gaps = 1/55 (1%)
Frame = -3
Query: 493 SSDQCQDEGS-CVICLEEYKNMDDVGTLKTCGHDYHVSCIKKWLSMKKLCPICKS 546
SSD GS CVICL E+ + + + L C H +HV CI KWL CP C++
Sbjct: 470 SSDSPNSSGSSCVICLAEFCDGEQIRFLPKCNHHFHVVCIDKWLLSHSSCPTCRN 306
>TC13875
Length = 462
Score = 52.4 bits (124), Expect = 2e-07
Identities = 22/60 (36%), Positives = 35/60 (57%)
Frame = +1
Query: 485 CVTETIYCSSDQCQDEGSCVICLEEYKNMDDVGTLKTCGHDYHVSCIKKWLSMKKLCPIC 544
C+ + +DQ SCVIC +Y++ D+ T+ +C H YH CI WL + ++CP+C
Sbjct: 4 CLQSSTRPGNDQHGSHDSCVICRVDYED-DESLTVLSCQHLYHPECINHWLKINQVCPVC 180
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.313 0.128 0.385
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,081,200
Number of Sequences: 28460
Number of extensions: 180336
Number of successful extensions: 1004
Number of sequences better than 10.0: 138
Number of HSP's better than 10.0 without gapping: 968
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 980
length of query: 556
length of database: 4,897,600
effective HSP length: 95
effective length of query: 461
effective length of database: 2,193,900
effective search space: 1011387900
effective search space used: 1011387900
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 58 (26.9 bits)
Medicago: description of AC124963.24