BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC124963.22 + phase: 0 /pseudo
(516 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC19896 similar to UP|Q8W041 (Q8W041) Dihydrofolate synthetase /... 175 1e-44
AV779129 72 2e-13
TC7976 homologue to UP|METK_LYCES (P43280) S-adenosylmethionine ... 29 2.1
TC7975 homologue to UP|Q9AT56 (Q9AT56) S-adenosyl-L-methionine s... 29 2.1
TC14110 homologue to UP|Q9AT55 (Q9AT55) S-adenosyl-L-methionine ... 29 2.1
AU089302 28 3.6
TC8009 homologue to UP|Q9LKJ2 (Q9LKJ2) Cytosolic phosphoglycerat... 28 3.6
TC10304 homologue to UP|METM_LYCES (P43282) S-adenosylmethionine... 27 6.2
AW720310 27 8.0
TC16882 homologue to UP|H2A_CICAR (O65759) Histone H2A, partial ... 27 8.0
>TC19896 similar to UP|Q8W041 (Q8W041) Dihydrofolate synthetase
/folylpolyglutamate synthetase (Folylpolyglutamate
synthase) (Folylpoly-gamma-glutamate synthetase) (FPGS)
, partial (16%)
Length = 563
Score = 175 bits (444), Expect = 1e-44
Identities = 92/132 (69%), Positives = 99/132 (74%), Gaps = 23/132 (17%)
Frame = +1
Query: 1 MRSVRTLCSHREDSEMKDLVDYIDSLKNYEKYGVPTGAGTDSNDGFDLGRMRRLLDRFGN 60
M S+R +CS +ED EMKDL+DYIDSLKNYEK GVPTGAGTDS+DGFDL RM RL+D FGN
Sbjct: 166 MGSLRAMCSRQEDPEMKDLLDYIDSLKNYEKSGVPTGAGTDSDDGFDLRRMTRLMDHFGN 345
Query: 61 PHSKFK----------------------TEGYSVGCYSSPHIQTIRERI-LGRSGDPVSA 97
PHSKFK TE YSVGCY+SPHIQTIRERI LGRSGDPVSA
Sbjct: 346 PHSKFKAVHIAGTKGKGSTAAFISNILRTERYSVGCYTSPHIQTIRERILLGRSGDPVSA 525
Query: 98 KLLNNLFHRIKQ 109
KLLN LFHRIKQ
Sbjct: 526 KLLNGLFHRIKQ 561
>AV779129
Length = 556
Score = 72.4 bits (176), Expect = 2e-13
Identities = 41/73 (56%), Positives = 48/73 (65%), Gaps = 3/73 (4%)
Frame = -3
Query: 428 RDSWIKASEELGTDICHDGMTEYRELF--KEQPVSSESNL-TDGKTILATESSLKDCLRM 484
RDSWIKASEE+G ICH+G+ EY EL KEQ +SSESNL DGKT+L TES K
Sbjct: 554 RDSWIKASEEIGIGICHEGLEEYSELLKEKEQSLSSESNLGDDGKTMLVTESLFKGLFEN 375
Query: 485 ANEILNRRRDEKG 497
N++R KG
Sbjct: 374 CK*DSNQKRG*KG 336
Score = 61.2 bits (147), Expect = 4e-10
Identities = 31/39 (79%), Positives = 34/39 (86%)
Frame = -1
Query: 477 SLKDCLRMANEILNRRRDEKGVIVITGSLHIVSSVLASL 515
SLK CLR AN+IL RR DEK V+VITGSLHIVSSVLAS+
Sbjct: 397 SLKGCLRTANKILTRRGDEKDVVVITGSLHIVSSVLASI 281
>TC7976 homologue to UP|METK_LYCES (P43280) S-adenosylmethionine synthetase
1 (Methionine adenosyltransferase 1) (AdoMet synthetase
1) , partial (67%)
Length = 862
Score = 28.9 bits (63), Expect = 2.1
Identities = 14/35 (40%), Positives = 18/35 (51%)
Frame = +2
Query: 241 SFSILNGKPCQICDIEIQTVKDLKLAMDPDPRCAC 275
S S+ G P ++CD V D L DPD + AC
Sbjct: 86 SESVNEGHPDKLCDQISDAVLDACLEQDPDSKVAC 190
>TC7975 homologue to UP|Q9AT56 (Q9AT56) S-adenosyl-L-methionine synthetase
(S-adenosylmethionine synthetase) (Methionine
adenosyltransferase) (AdoMet synthetase) , complete
Length = 1564
Score = 28.9 bits (63), Expect = 2.1
Identities = 14/35 (40%), Positives = 18/35 (51%)
Frame = +1
Query: 241 SFSILNGKPCQICDIEIQTVKDLKLAMDPDPRCAC 275
S S+ G P ++CD V D L DPD + AC
Sbjct: 118 SESVNEGHPDKLCDQISDAVLDACLEQDPDSKVAC 222
>TC14110 homologue to UP|Q9AT55 (Q9AT55) S-adenosyl-L-methionine synthetase
(S-adenosylmethionine synthetase) (Methionine
adenosyltransferase) (AdoMet synthetase) , complete
Length = 1576
Score = 28.9 bits (63), Expect = 2.1
Identities = 14/35 (40%), Positives = 18/35 (51%)
Frame = +2
Query: 241 SFSILNGKPCQICDIEIQTVKDLKLAMDPDPRCAC 275
S S+ G P ++CD V D L DPD + AC
Sbjct: 119 SESVNEGHPDKLCDQISDAVLDACLEQDPDSKVAC 223
>AU089302
Length = 327
Score = 28.1 bits (61), Expect = 3.6
Identities = 13/35 (37%), Positives = 18/35 (51%)
Frame = +3
Query: 241 SFSILNGKPCQICDIEIQTVKDLKLAMDPDPRCAC 275
S S+ G P +ICD + D L DP+ + AC
Sbjct: 66 SESVNEGHPDKICDQVSDAILDACLEQDPESKVAC 170
>TC8009 homologue to UP|Q9LKJ2 (Q9LKJ2) Cytosolic phosphoglycerate kinase
, complete
Length = 1697
Score = 28.1 bits (61), Expect = 3.6
Identities = 46/176 (26%), Positives = 69/176 (39%), Gaps = 36/176 (20%)
Frame = +2
Query: 320 GVG*SVHTSLEGVSS*NLKKPRPWDFLEQQYC--LI*SVAHTKESAKALMNTIKMASP-- 375
G H S EGV+ KP FL Q+ L+ +V++ K+ A++ K++S
Sbjct: 629 GTAHRAHASTEGVAK--YLKPAVAGFLMQKELDYLVGAVSNPKKPFAAIVGGSKVSSKIG 802
Query: 376 -----------------------KARLAFVVAMASDKDHAGFAREILSDAYVKTVIL--- 409
KA+ V + ++D A +L A K V L
Sbjct: 803 VIESLLEKGDYLLLGGGMIYTFYKAQGLAVGSSLVEEDKLNLATSLLEKAKAKGVALLLP 982
Query: 410 TEAAIAG-----AVTRTAPASLLRDSWIKASEELGTDICHDGMTEYRE-LFKEQPV 459
T+ IA A ++T PAS + D W +G DI D + + E L K Q V
Sbjct: 983 TDVVIADKFSADANSKTVPASSIPDGW------MGLDIGPDSIKTFGEALDKTQTV 1132
>TC10304 homologue to UP|METM_LYCES (P43282) S-adenosylmethionine synthetase
3 (Methionine adenosyltransferase 3) (AdoMet synthetase
3) , partial (35%)
Length = 469
Score = 27.3 bits (59), Expect = 6.2
Identities = 12/35 (34%), Positives = 18/35 (51%)
Frame = +1
Query: 241 SFSILNGKPCQICDIEIQTVKDLKLAMDPDPRCAC 275
S S+ G P ++CD + D L DP+ + AC
Sbjct: 76 SESVNEGHPDKLCDQVSDAILDACLEQDPESKVAC 180
>AW720310
Length = 612
Score = 26.9 bits (58), Expect = 8.0
Identities = 15/44 (34%), Positives = 23/44 (52%), Gaps = 7/44 (15%)
Frame = +2
Query: 74 CYSSPHIQTIRERILGRSGD-------PVSAKLLNNLFHRIKQD 110
CY+ +++ I E I SGD P+ K L +L H +KQ+
Sbjct: 26 CYAICYLKNIGEAIFNFSGDKYMQLLIPIC*KKLKDLLHCLKQE 157
>TC16882 homologue to UP|H2A_CICAR (O65759) Histone H2A, partial (96%)
Length = 694
Score = 26.9 bits (58), Expect = 8.0
Identities = 15/37 (40%), Positives = 22/37 (58%), Gaps = 4/37 (10%)
Frame = +2
Query: 77 SPHIQTIRERILGRS----GDPVSAKLLNNLFHRIKQ 109
SP Q +R IL R+ G P+S+ ++ N FHR +Q
Sbjct: 2 SPLSQLLRALILDRNF*G*GFPISSSIVENEFHRRQQ 112
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.327 0.140 0.413
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,566,265
Number of Sequences: 28460
Number of extensions: 91137
Number of successful extensions: 453
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 449
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 449
length of query: 516
length of database: 4,897,600
effective HSP length: 94
effective length of query: 422
effective length of database: 2,222,360
effective search space: 937835920
effective search space used: 937835920
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 57 (26.6 bits)
Medicago: description of AC124963.22