BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC124963.21 + phase: 0 /pseudo
(739 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV780221 79 3e-15
AV767121 71 5e-13
TC18710 62 3e-10
BP060613 45 4e-05
AV780705 44 9e-05
TC13572 27 0.11
AV426437 31 0.62
TC10115 similar to UP|Q9SSQ3 (Q9SSQ3) F6D8.27 protein (Epoxide ... 31 0.81
TC9368 UP|Q41211 (Q41211) Actin (Fragment), partial (89%) 30 1.4
TC16857 similar to PIR|T51729|T51729 arginine-tRNA-protein trans... 29 2.4
TC19164 29 2.4
CB828813 28 4.0
TC15402 UP|CAE45587 (CAE45587) Vacuolar proton-ATPase subunit-li... 28 5.3
AV777513 28 5.3
TC14319 similar to UP|AP23_ARATH (P42736) AP2 domain transcripti... 28 6.9
AV775857 28 6.9
TC8156 UP|AAR99188 (AAR99188) ATP synthase F0 subunit 8, partial... 27 9.0
>AV780221
Length = 461
Score = 79.0 bits (193), Expect = 3e-15
Identities = 41/108 (37%), Positives = 58/108 (52%), Gaps = 6/108 (5%)
Frame = +3
Query: 393 FFCEGSDNHKKVTWVDWNFVCLSKEVGGYGVRRIREFNSALLGKWCWQLLIEKESLWYRV 452
FF + KK+ WV W+ VC K+ GG GV+ + FN ALLGKW W++L E++ LWY+V
Sbjct: 111 FFGREVEGGKKIAWVKWSTVCRPKDEGGLGVQNLGLFNKALLGKWRWRMLKERDGLWYKV 290
Query: 453 LSARYNEEGGRLLDGGRSTSAWWRVIATLQREE----WFNS--CREIG 494
L +Y +S S+WW + + E+ W CR IG
Sbjct: 291 LLIKYKNA------IPQSASSWWNDLHSTCFEDGGGGWMQRGLCRRIG 416
>AV767121
Length = 525
Score = 71.2 bits (173), Expect = 5e-13
Identities = 42/111 (37%), Positives = 61/111 (54%), Gaps = 4/111 (3%)
Frame = +2
Query: 393 FFCEGSDNHKKVTWVDWNFVCLSKEVGGYGVRRIREFNSALLGKWCWQLLIEKESLWYRV 452
F GS+N K+ V W VC KE+GG G++ + FN ALLGK W+ L E +SLW RV
Sbjct: 149 FLWGGSENENKIA*VKWTDVCKPKELGGLGIKDLFTFNKALLGK*RWRYLTEPDSLWRRV 328
Query: 453 LSARYNEEGGRLLDGGRSTSAWWRVIATLQREE---WFNS-CREIGGEWER 499
+ A+ D S+WW I +L E+ WF+S +++ GE ++
Sbjct: 329 IEAQ--------PDHYSCGSSWWNDILSLCPEDADGWFSSGLKKLVGEGDQ 457
>TC18710
Length = 843
Score = 62.4 bits (150), Expect = 3e-10
Identities = 38/134 (28%), Positives = 67/134 (49%), Gaps = 3/134 (2%)
Frame = +2
Query: 72 LNDFCLISLVESMYKILAKVLANRLRYVIGMVISDSQSTFVKGRQILDGILVANEVVDDA 131
+N F IS +YK+++K+ RL+ + +IS QS F++GRQI D +L+ E
Sbjct: 350 INQFRPISGCTFLYKVISKIFVARLKDAMPDLISPMQSGFIQGRQIQDNLLIVQEAFHAI 529
Query: 132 HR---CKKELLLFKVDFEEAYDIVDWSYLDEVMYKMGFPVL*QK*IKECIGTATTSMLVN 188
+R + + K+D +AYD V+W +L+ + GF K I + + + +N
Sbjct: 530 NRPGALGRNHSIIKLDMNKAYDRVEWKFLESSLLAFGFSTNWVKMIMILVSGVSYNYKIN 709
Query: 189 GSPTDEFPLKRGCK 202
G + +RG +
Sbjct: 710 GVVGPKLLPQRGLR 751
>BP060613
Length = 378
Score = 45.1 bits (105), Expect = 4e-05
Identities = 23/77 (29%), Positives = 37/77 (47%)
Frame = +1
Query: 398 SDNHKKVTWVDWNFVCLSKEVGGYGVRRIREFNSALLGKWCWQLLIEKESLWYRVLSARY 457
S + V W W+ + K+ GG G + N ALL K W++L ++LW ++L A Y
Sbjct: 55 SKGTRGVHWRKWDLLTELKDEGGLGFKDFEIQNQALLAKQAWRILHNPDALWVQILKALY 234
Query: 458 NEEGGRLLDGGRSTSAW 474
L R+ ++W
Sbjct: 235 FPHHDFLQTTKRTGASW 285
>AV780705
Length = 524
Score = 43.9 bits (102), Expect = 9e-05
Identities = 20/44 (45%), Positives = 27/44 (60%)
Frame = -2
Query: 414 LSKEVGGYGVRRIREFNSALLGKWCWQLLIEKESLWYRVLSARY 457
LSKE GG G R +R N A L + W++L E+LW RV+ + Y
Sbjct: 193 LSKEKGGVGFRDLRTQNLAFLARQAWRVLTNPEALWVRVMKSLY 62
>TC13572
Length = 534
Score = 26.6 bits (57), Expect(2) = 0.11
Identities = 11/19 (57%), Positives = 12/19 (62%)
Frame = -1
Query: 430 NSALLGKWCWQLLIEKESL 448
N ALLGKW W+L SL
Sbjct: 534 NKALLGKWLWRLKTVPNSL 478
Score = 25.8 bits (55), Expect(2) = 0.11
Identities = 16/42 (38%), Positives = 21/42 (49%), Gaps = 3/42 (7%)
Frame = -3
Query: 449 WYRVLSARYNEEGGRLLDGG-RSTSAWWRVIATLQREE--WF 487
W RV+ A+Y DGG + S+WWR + T E WF
Sbjct: 478 WCRVVLAKYG-------DGGCTNISSWWRDVQTAGGGENGWF 374
>AV426437
Length = 303
Score = 31.2 bits (69), Expect = 0.62
Identities = 9/26 (34%), Positives = 17/26 (64%)
Frame = +1
Query: 399 DNHKKVTWVDWNFVCLSKEVGGYGVR 424
+N +K+ W++W +C K + G GV+
Sbjct: 214 ENQRKIAWINWETICKPKWLRGLGVK 291
>TC10115 similar to UP|Q9SSQ3 (Q9SSQ3) F6D8.27 protein (Epoxide hydrolase)
, partial (18%)
Length = 489
Score = 30.8 bits (68), Expect = 0.81
Identities = 12/36 (33%), Positives = 17/36 (46%)
Frame = -1
Query: 474 WWRVIATLQREEWFNSCREIGGEWERNILLVRCLGW 509
WW ++ W+ + R G EWER + LGW
Sbjct: 171 WWWIMVFFYNSWWWKTLR--GSEWERTLQKTPFLGW 70
>TC9368 UP|Q41211 (Q41211) Actin (Fragment), partial (89%)
Length = 592
Score = 30.0 bits (66), Expect = 1.4
Identities = 14/39 (35%), Positives = 23/39 (58%)
Frame = -1
Query: 327 VVCLFGNH*LGGSKLDCQGGKLNIYLLVVIWFC*NLSCH 365
++CL H L G++ CQ G N ++ +W+C NL+ H
Sbjct: 268 ILCLCNPHLLEGTE-GCQNGASNPDTVLPLWWCHNLNFH 155
>TC16857 similar to PIR|T51729|T51729 arginine-tRNA-protein transferase 1
[imported] - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (3%)
Length = 802
Score = 29.3 bits (64), Expect = 2.4
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = +2
Query: 483 REEWFNSCREIGGEWERNILLVRCL 507
R+ W SCR IGG WE+ L C+
Sbjct: 350 RDTWNPSCRNIGGLWEQCYLSEWCI 424
>TC19164
Length = 531
Score = 29.3 bits (64), Expect = 2.4
Identities = 19/54 (35%), Positives = 29/54 (53%)
Frame = +2
Query: 348 LNIYLLVVIWFC*NLSCHLSLFMLFLSSRLLQVLSPLLNLF*NVFFFCEGSDNH 401
+++Y +VI C* + SLF++++ +R L L L V F CE S NH
Sbjct: 263 MSVYKALVIDLC*YI*HPASLFIIYVCARKLSRK*DELQLVFEV*FKCELSSNH 424
>CB828813
Length = 465
Score = 28.5 bits (62), Expect = 4.0
Identities = 19/40 (47%), Positives = 23/40 (57%)
Frame = -2
Query: 523 VGGGQMGVCVRYVSVGVG*EW*GVEVEEEVVCMGGGEGGG 562
+GGG G VG+G G+EVEE V +GGG GGG
Sbjct: 326 LGGGLGGG-----GVGIG----GLEVEEVRVGVGGGGGGG 234
>TC15402 UP|CAE45587 (CAE45587) Vacuolar proton-ATPase subunit-like protein,
complete
Length = 2423
Score = 28.1 bits (61), Expect = 5.3
Identities = 17/47 (36%), Positives = 23/47 (48%)
Frame = -3
Query: 358 FC*NLSCHLSLFMLFLSSRLLQVLSPLLNLF*NVFFFCEGSDNHKKV 404
F NL HLSLF FLS R+ + + F F G ++HK +
Sbjct: 837 FSRNLCNHLSLFSQFLSKRVAISTKSITDFQNLCFDFFTGIEDHKHI 697
>AV777513
Length = 334
Score = 28.1 bits (61), Expect = 5.3
Identities = 14/33 (42%), Positives = 17/33 (51%)
Frame = -2
Query: 542 EW*GVEVEEEVVCMGGGEGGGTLFITSKCNFAG 574
EW GV V E C G GGG + + S+ N G
Sbjct: 180 EWAGVRVLESEECGGVDGGGGEIQVQSQGNVGG 82
>TC14319 similar to UP|AP23_ARATH (P42736) AP2 domain transcription factor
RAP2.3 (Related to AP2 protein 3) (Cadmium-induced
protein AS30), partial (37%)
Length = 1130
Score = 27.7 bits (60), Expect = 6.9
Identities = 13/39 (33%), Positives = 19/39 (48%)
Frame = +2
Query: 32 GIFCVIMLCVLLVNFIGMGGLPKESIALSLLLFLRLRAR 70
G+FC++M + + NF+G K S SL L R
Sbjct: 926 GVFCLLMSLINIPNFLGFSDCIKLSYLCSLFLLFSFVGR 1042
>AV775857
Length = 503
Score = 27.7 bits (60), Expect = 6.9
Identities = 17/43 (39%), Positives = 24/43 (55%), Gaps = 5/43 (11%)
Frame = -3
Query: 31 FGIFCVIMLCVLLVNFIGMGGLPKESIA-----LSLLLFLRLR 68
F + C +CV+L + +GM GL S A L +LL+LR R
Sbjct: 426 FSLACKRSMCVVLSSLLGMEGLVP*SFADCTLLL*ILLYLRCR 298
>TC8156 UP|AAR99188 (AAR99188) ATP synthase F0 subunit 8, partial (24%)
Length = 635
Score = 27.3 bits (59), Expect = 9.0
Identities = 11/33 (33%), Positives = 20/33 (60%), Gaps = 1/33 (3%)
Frame = -1
Query: 464 LLDGGRSTS-AWWRVIATLQREEWFNSCREIGG 495
L+ G R + WW ++ATL + W+ + +E+ G
Sbjct: 548 LIHGARHGALGWWLLLATLNLKRWWLAWKEVRG 450
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.350 0.157 0.564
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 15,593,820
Number of Sequences: 28460
Number of extensions: 271176
Number of successful extensions: 2846
Number of sequences better than 10.0: 34
Number of HSP's better than 10.0 without gapping: 2776
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2836
length of query: 739
length of database: 4,897,600
effective HSP length: 97
effective length of query: 642
effective length of database: 2,136,980
effective search space: 1371941160
effective search space used: 1371941160
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 14 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 38 (21.9 bits)
S2: 59 (27.3 bits)
Medicago: description of AC124963.21