BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC124963.14 + phase: 0
(294 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP040410 69 7e-13
BP035958 35 0.011
TC8312 similar to UP|Q8S901 (Q8S901) Syringolide-induced protein... 35 0.015
TC17990 similar to GB|AAM65958.1|21594040|AY088421 homogentisate... 34 0.026
CB826886 34 0.026
TC8576 similar to UP|Q9SJN3 (Q9SJN3) At2g36220 protein (At2g3622... 33 0.057
TC15394 similar to UP|Q9FFQ8 (Q9FFQ8) Emb|CAB62360.1, partial (16%) 33 0.074
BP037634 32 0.097
TC9186 homologue to UP|Q9BIW6 (Q9BIW6) Brahma-associated protein... 32 0.13
TC13056 similar to UP|IF38_MEDTR (Q9XHM1) Eukaryotic translation... 32 0.17
TC17654 similar to UP|BAC36925 (BAC36925) 8 days embryo whole bo... 31 0.28
TC8089 similar to UP|FLA1_ARATH (Q9FM65) Fasciclin-like arabinog... 31 0.28
BP063112 30 0.37
TC14478 weakly similar to UP|Q94G86 (Q94G86) Beta-1,3-glucanase-... 30 0.37
TC12236 similar to UP|Q9LJE2 (Q9LJE2) Lysyl-tRNA synthetase, par... 30 0.48
AV768333 30 0.63
TC17275 30 0.63
BU494405 29 0.82
TC17096 similar to PIR|C53394|C53394 ribosomal protein L12.C pre... 29 0.82
TC13923 homologue to UP|Q38950 (Q38950) Protein phosphatase 2A 6... 29 0.82
>BP040410
Length = 486
Score = 69.3 bits (168), Expect = 7e-13
Identities = 46/104 (44%), Positives = 59/104 (56%), Gaps = 7/104 (6%)
Frame = -2
Query: 191 ETESTSID-----DNEESSSQSQKRESVSDKEDSIGERIGLTKDLEN--VQELLLKGRVK 243
ETESTS D DN+ S + ES + + ++ K+LE+ +++ +
Sbjct: 485 ETESTSGDTSGDNDNKPVSVRHSVSESEAKRVSESETKMEFFKELEDSLLRQGITASITN 306
Query: 244 KEEEDSAVARPVVLTRCKSEPARVSYRIDPEVNSNLWKKTRLGF 287
E +SA PVVLTRCKSEPA RIDPEVN NLWKK RLGF
Sbjct: 305 NSEGNSASRSPVVLTRCKSEPANRGLRIDPEVN-NLWKKRRLGF 177
Score = 27.7 bits (60), Expect = 2.4
Identities = 16/51 (31%), Positives = 28/51 (54%)
Frame = -2
Query: 121 SESNRNSNFTLEDEQRVSVSVESVDNAIVSNSNCSTPPKNALLLTRCRSAP 171
SES F E E S+ + + +I +NS ++ ++ ++LTRC+S P
Sbjct: 389 SESETKMEFFKELED--SLLRQGITASITNNSEGNSASRSPVVLTRCKSEP 243
>BP035958
Length = 574
Score = 35.4 bits (80), Expect = 0.011
Identities = 31/87 (35%), Positives = 41/87 (46%), Gaps = 8/87 (9%)
Frame = +1
Query: 5 TTKSISSPARTDKF--PPPLMRFLRTNAGSRSSRRSRSSPMFVLRNKKT------IETTQ 56
T + SP+R ++ PPP GS SS RSRSS LR+ KT I TT
Sbjct: 139 TPATSPSPSRRSRWRPPPPPPNPTPPA*GSSSSARSRSSKSR-LRSSKTLPSASSIHTTS 315
Query: 57 EPSSPKVTCMGQVRAKRSSSKSSTTPT 83
SSP T + R + ++ S +PT
Sbjct: 316 PTSSPSATSRRRHRRRSTAPTPSASPT 396
>TC8312 similar to UP|Q8S901 (Q8S901) Syringolide-induced protein 14-1-1,
partial (46%)
Length = 1358
Score = 35.0 bits (79), Expect = 0.015
Identities = 40/163 (24%), Positives = 67/163 (40%)
Frame = +2
Query: 56 QEPSSPKVTCMGQVRAKRSSSKSSTTPTHHWWIKKPNPCRCRLVWPKWAFFRRKKPTKPK 115
+EP+SPK++CMGQ++ K++ K S + + N R K+A K T
Sbjct: 398 REPTSPKISCMGQIKHKKNQIKKSKSKKS---VSLQNETR------KFASTTNKTTTTST 550
Query: 116 QDSVKSESNRNSNFTLEDEQRVSVSVESVDNAIVSNSNCSTPPKNALLLTRCRSAPYRSS 175
+S + N QR+ + S+ S+ + A +++ + R S
Sbjct: 551 SNSTDVIEVKKKN-VASKFQRMLFHAAKQPKSAGRKSDASSAEREAPHMSQMK----RFS 715
Query: 176 SLASRFWSSPLRNEEETESTSIDDNEESSSQSQKRESVSDKED 218
S F S + E E IDD S + + ES D+E+
Sbjct: 716 SGRDAFSSFDWKAEMAAEEIHIDD--YYSDEDRVVESDGDEEE 838
>TC17990 similar to GB|AAM65958.1|21594040|AY088421 homogentisate
1,2-dioxygenase {Arabidopsis thaliana;} , partial (28%)
Length = 561
Score = 34.3 bits (77), Expect = 0.026
Identities = 15/39 (38%), Positives = 22/39 (55%)
Frame = +2
Query: 55 TQEPSSPKVTCMGQVRAKRSSSKSSTTPTHHWWIKKPNP 93
T EP P+V C G++ ++ + S SST PT W +P
Sbjct: 299 THEPFKPRVPCNGRILSEFNHSNSSTNPTQLRWKPMDSP 415
>CB826886
Length = 631
Score = 34.3 bits (77), Expect = 0.026
Identities = 15/39 (38%), Positives = 22/39 (55%)
Frame = +3
Query: 55 TQEPSSPKVTCMGQVRAKRSSSKSSTTPTHHWWIKKPNP 93
T EP P+V C G++ ++ + S SST PT W +P
Sbjct: 285 THEPFKPRVPCNGRILSEFNHSNSSTNPTQLRWKPMDSP 401
>TC8576 similar to UP|Q9SJN3 (Q9SJN3) At2g36220 protein
(At2g36220/F2H17.17), partial (17%)
Length = 604
Score = 33.1 bits (74), Expect = 0.057
Identities = 20/66 (30%), Positives = 33/66 (49%), Gaps = 3/66 (4%)
Frame = +1
Query: 8 SISSPARTDKFPPPLMRFLRTNAGSRSSRRSRSSPMFVLRN---KKTIETTQEPSSPKVT 64
S++ +R P P R+ + + +F R+ K++ + EPSSPKV+
Sbjct: 406 SLNLKSRIIGLPKPQKPSFTDAKNRRNHKPCNNIKLFPKRSVSVTKSVTSHAEPSSPKVS 585
Query: 65 CMGQVR 70
CMG+VR
Sbjct: 586 CMGRVR 603
>TC15394 similar to UP|Q9FFQ8 (Q9FFQ8) Emb|CAB62360.1, partial (16%)
Length = 1093
Score = 32.7 bits (73), Expect = 0.074
Identities = 15/55 (27%), Positives = 31/55 (56%)
Frame = +2
Query: 24 RFLRTNAGSRSSRRSRSSPMFVLRNKKTIETTQEPSSPKVTCMGQVRAKRSSSKS 78
+ ++ +GS++ R++S+ +++ K ++ T + S+PK T A S SKS
Sbjct: 140 KIVKEGSGSKAGERTKSAKKTPVKSSKNVDKTPKKSTPKQTAAEHDSASASLSKS 304
>BP037634
Length = 575
Score = 32.3 bits (72), Expect = 0.097
Identities = 26/75 (34%), Positives = 38/75 (50%), Gaps = 5/75 (6%)
Frame = +3
Query: 19 PPPLMRFLRTNAGSRSSRRSRSSPMFVLRNKKTIETTQEPSSP-----KVTCMGQVRAKR 73
PP L +FL SR S+ S + F LRN +T TT+ P K + + AK+
Sbjct: 6 PPKLCKFL*PIQHSRPSKSSHFTNQFSLRNHRT--TTKFRCQPNSMPLKGRAICPISAKQ 179
Query: 74 SSSKSSTTPTHHWWI 88
S +K+ST+ H +I
Sbjct: 180SITKNSTS*RSHRFI 224
>TC9186 homologue to UP|Q9BIW6 (Q9BIW6) Brahma-associated protein 111kD,
partial (3%)
Length = 618
Score = 32.0 bits (71), Expect = 0.13
Identities = 13/25 (52%), Positives = 19/25 (76%)
Frame = +1
Query: 57 EPSSPKVTCMGQVRAKRSSSKSSTT 81
EP SPKV+C+G+VR+KR ++ T
Sbjct: 460 EPLSPKVSCIGRVRSKRDRNRRLRT 534
>TC13056 similar to UP|IF38_MEDTR (Q9XHM1) Eukaryotic translation initiation
factor 3 subunit 8 (eIF3 p110) (eIF3c), partial (12%)
Length = 607
Score = 31.6 bits (70), Expect = 0.17
Identities = 20/75 (26%), Positives = 37/75 (48%)
Frame = +2
Query: 176 SLASRFWSSPLRNEEETESTSIDDNEESSSQSQKRESVSDKEDSIGERIGLTKDLENVQE 235
++ASRFW+ + EE ES D+ E+ + + VS+ E G + D ++ Q+
Sbjct: 110 TMASRFWTQGGSDSEEEESDR-DEEVETVAVESAGQDVSNSNKYYAENEGNSDDSDDDQK 286
Query: 236 LLLKGRVKKEEEDSA 250
+++ K E+ A
Sbjct: 287 RVVRSAKDKRFEEMA 331
>TC17654 similar to UP|BAC36925 (BAC36925) 8 days embryo whole body cDNA,
RIKEN full-length enriched library, clone:5730499P16
product:PHOSPHATIDYLINOSITOL 3-KINASE CATALYTIC SUBUNIT,
BETA ISOFORM (PI3-KINASE P110 SUBUNIT BETA)
(PTDINS-3-KINASE P110) (PI3K) (PI3 , partial (8%)
Length = 549
Score = 30.8 bits (68), Expect = 0.28
Identities = 26/88 (29%), Positives = 33/88 (36%)
Frame = +3
Query: 10 SSPARTDKFPPPLMRFLRTNAGSRSSRRSRSSPMFVLRNKKTIETTQEPSSPKVTCMGQV 69
SSP K P R+ + S S RSRS LRNK SPK +
Sbjct: 27 SSPRSPAKSPAKSASRSRSRSRSLSGSRSRSKSPLPLRNK----------SPKRCSASPI 176
Query: 70 RAKRSSSKSSTTPTHHWWIKKPNPCRCR 97
R S + S +P + P+ R R
Sbjct: 177 RRSASPQRRSPSPQRRSASRSPSRSRSR 260
>TC8089 similar to UP|FLA1_ARATH (Q9FM65) Fasciclin-like arabinogalactan
protein 1 precursor, partial (43%)
Length = 890
Score = 30.8 bits (68), Expect = 0.28
Identities = 24/87 (27%), Positives = 37/87 (41%)
Frame = +2
Query: 10 SSPARTDKFPPPLMRFLRTNAGSRSSRRSRSSPMFVLRNKKTIETTQEPSSPKVTCMGQV 69
SSP T P P TN G +S ++P + ++ T ++ +S T
Sbjct: 263 SSPPSTTTSPSPTSPPRSTN-GPQSPSAPSTTPPWTTSSQNTHQSPPSRTSSPSTSSSTT 439
Query: 70 RAKRSSSKSSTTPTHHWWIKKPNPCRC 96
A RSS++S T P P+P +C
Sbjct: 440 SAPRSSTRSPTAP--------PSPPQC 496
>BP063112
Length = 530
Score = 30.4 bits (67), Expect = 0.37
Identities = 13/25 (52%), Positives = 15/25 (60%)
Frame = +3
Query: 74 SSSKSSTTPTHHWWIKKPNPCRCRL 98
SSS S P HH W++ P RCRL
Sbjct: 21 SSSDSWRQPNHHHWLRIAPPKRCRL 95
>TC14478 weakly similar to UP|Q94G86 (Q94G86) Beta-1,3-glucanase-like
protein, partial (83%)
Length = 1784
Score = 30.4 bits (67), Expect = 0.37
Identities = 34/129 (26%), Positives = 45/129 (34%), Gaps = 16/129 (12%)
Frame = +2
Query: 2 KLSTTKSISSPARTDKFPPPLMRFLRTNAGSRSSR--------RSRSSPMFVLRNKKTIE 53
K TT SP T PPP + T RSSR S + P + I
Sbjct: 164 KSPTTFHRQSPPSTSSDPPPSEKSASTAPIPRSSRPSPIPESESSSAPPTATFQISPPIP 343
Query: 54 T--------TQEPSSPKVTCMGQVRAKRSSSKSSTTPTHHWWIKKPNPCRCRLVWPKWAF 105
T T P++P T + A R S ++ T W ++ CR R P A
Sbjct: 344 TPQFSG*TPTSYPTTPPATSLSSPSATRYSPPATPTSPRS-WSRRFGMCRARSPPPPSAA 520
Query: 106 FRRKKPTKP 114
R + P
Sbjct: 521 RLRSRRCSP 547
>TC12236 similar to UP|Q9LJE2 (Q9LJE2) Lysyl-tRNA synthetase, partial
(16%)
Length = 429
Score = 30.0 bits (66), Expect = 0.48
Identities = 24/63 (38%), Positives = 31/63 (49%)
Frame = +2
Query: 21 PLMRFLRTNAGSRSSRRSRSSPMFVLRNKKTIETTQEPSSPKVTCMGQVRAKRSSSKSST 80
PL FL +++ S SS R +P + + T SSP GQ R RSS+ SST
Sbjct: 20 PLKLFLHSHSSSISSTAFRRTPRRRVLRSSSSSTATTASSP-----GQNR--RSSASSST 178
Query: 81 TPT 83
T T
Sbjct: 179TST 187
>AV768333
Length = 493
Score = 29.6 bits (65), Expect = 0.63
Identities = 27/90 (30%), Positives = 37/90 (41%), Gaps = 1/90 (1%)
Frame = +2
Query: 4 STTKSISSPARTDKFPPPLMRFLRTNAGSRS-SRRSRSSPMFVLRNKKTIETTQEPSSPK 62
+TT S+P T K PL + +S S ++ + LR TT S K
Sbjct: 224 TTTSPFSTPLSTSKNSEPLSHHTPPSTSLKSPSTAPTTASLRTLRTPTPSPTTSFFHSSK 403
Query: 63 VTCMGQVRAKRSSSKSSTTPTHHWWIKKPN 92
Q+ + SSKSS T + W KK N
Sbjct: 404 P----QLHSNPHSSKSSNTSSTTSWKKKKN 481
>TC17275
Length = 796
Score = 29.6 bits (65), Expect = 0.63
Identities = 29/148 (19%), Positives = 68/148 (45%), Gaps = 25/148 (16%)
Frame = +2
Query: 108 RKKPTKP------KQDSVKSESNRNSNFTLEDEQR-------------VSVSVESVDN-- 146
+KKPTKP ++ KS + N+N + +++ + S+ ++ ++N
Sbjct: 206 KKKPTKPITTNLSSKNQTKSIKSNNNNLSSKNQTKTTLNASTFKKLNSTSIKIKKLNNST 385
Query: 147 --AIVSNSNCSTPPKNALLLTRCRSAPYRSSSLASRFWSSPLRNEEETESTSIDDNEESS 204
+ ++S ST K L LT+ A ++ + + ++ +N+ + I++ + +
Sbjct: 386 SKSFTNSSKTSTTSKKQLDLTKLAGAIAKNKTTKAT-TATDTKNKIKVNPGHIEEEKLNK 562
Query: 205 SQSQKRESVSD--KEDSIGERIGLTKDL 230
++K++S + ED + + KDL
Sbjct: 563 QSNKKKQSPPNWMFEDEDSDLVSDFKDL 646
>BU494405
Length = 563
Score = 29.3 bits (64), Expect = 0.82
Identities = 19/76 (25%), Positives = 34/76 (44%), Gaps = 13/76 (17%)
Frame = +1
Query: 221 GERIGLTKDLENVQELLLKGR-----------VKKEEED--SAVARPVVLTRCKSEPARV 267
G +GL LE + ++L+K R KKE D + RP+ L+R + +
Sbjct: 181 GGEVGLANILEGIVDVLVKDRHDLSYLFQKPVSKKEAPDYFDIIERPMDLSRIRERVRNM 360
Query: 268 SYRIDPEVNSNLWKKT 283
Y+ + ++W+ T
Sbjct: 361 EYKSREDFRHDIWQIT 408
>TC17096 similar to PIR|C53394|C53394 ribosomal protein L12.C precursor,
chloroplast - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (74%)
Length = 677
Score = 29.3 bits (64), Expect = 0.82
Identities = 18/54 (33%), Positives = 26/54 (47%), Gaps = 2/54 (3%)
Frame = +3
Query: 42 PMFVLRNK-KTIETTQEPSS-PKVTCMGQVRAKRSSSKSSTTPTHHWWIKKPNP 93
P LR K T+Q PS+ P + ++S S ++T+P HW KP P
Sbjct: 48 PHLTLRTTPKPPSTSQHPSAAPSASAPSPPSPRKSRSSATTSPASHW--NKPRP 203
>TC13923 homologue to UP|Q38950 (Q38950) Protein phosphatase 2A 65 kDa
regulatory subunit, partial (20%)
Length = 515
Score = 29.3 bits (64), Expect = 0.82
Identities = 22/59 (37%), Positives = 30/59 (50%)
Frame = -1
Query: 125 RNSNFTLEDEQRVSVSVESVDNAIVSNSNCSTPPKNALLLTRCRSAPYRSSSLASRFWS 183
R S+ T VS +V+SV + CSTPP N + T SA RS+S +S +S
Sbjct: 494 RQSDSTALSLTHVSSTVQSVSKGGSNTWACSTPPTNG-MKTPNSSAIARSTSSSSSLFS 321
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.310 0.124 0.356
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,689,429
Number of Sequences: 28460
Number of extensions: 87584
Number of successful extensions: 610
Number of sequences better than 10.0: 76
Number of HSP's better than 10.0 without gapping: 587
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 606
length of query: 294
length of database: 4,897,600
effective HSP length: 90
effective length of query: 204
effective length of database: 2,336,200
effective search space: 476584800
effective search space used: 476584800
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 55 (25.8 bits)
Medicago: description of AC124963.14