BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC124218.7 + phase: 0 /partial
(281 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC11704 similar to UP|O80812 (O80812) T8F5.19 protein, partial (9%) 89 8e-19
AW720269 67 3e-12
AV776921 63 5e-11
TC10604 similar to AAQ89671 (AAQ89671) At4g33460, partial (36%) 55 1e-08
TC11559 similar to UP|Q9SXT3 (Q9SXT3) Multidrug resistance prote... 55 2e-08
TC9999 similar to UP|Q9FMU0 (Q9FMU0) Similarity to ABC transport... 53 5e-08
TC11425 similar to UP|Q9M1Q9 (Q9M1Q9) P-glycoprotein-like proeti... 50 3e-07
TC18135 homologue to UP|Q9MAY4 (Q9MAY4) ABC transporter homolog,... 49 7e-07
BG662257 49 1e-06
AV767093 48 2e-06
CB827630 46 6e-06
TC18647 similar to UP|Q9ASR9 (Q9ASR9) At2g01320/F10A8.20, partia... 42 1e-04
TC15722 similar to UP|Q9FMU0 (Q9FMU0) Similarity to ABC transpor... 42 1e-04
AV416268 42 2e-04
AV417384 39 0.001
TC13598 similar to UP|Q9M1Q9 (Q9M1Q9) P-glycoprotein-like proeti... 36 0.006
TC16623 weakly similar to UP|TAP2_HUMAN (Q03519) Antigen peptide... 36 0.008
TC9119 homologue to AAQ65167 (AAQ65167) At1g64550, partial (20%) 33 0.071
TC15900 similar to UP|Q8GU54 (Q8GU54) SMC3 protein, partial (15%) 32 0.16
AU089197 30 0.46
>TC11704 similar to UP|O80812 (O80812) T8F5.19 protein, partial (9%)
Length = 556
Score = 89.0 bits (219), Expect = 8e-19
Identities = 44/51 (86%), Positives = 47/51 (91%), Gaps = 1/51 (1%)
Frame = +2
Query: 232 QHSTIKRAIDRLLFLHKGKLVWEGMTHEFTTST-NPIVQQFASGSLDGPIK 281
QHSTIKRA+DRLLFLH+GKLVWEGMTHEFTTST N IVQQFASG+ GPIK
Sbjct: 2 QHSTIKRAVDRLLFLHEGKLVWEGMTHEFTTSTENKIVQQFASGNRVGPIK 154
>AW720269
Length = 524
Score = 67.4 bits (163), Expect = 3e-12
Identities = 53/165 (32%), Positives = 80/165 (48%), Gaps = 6/165 (3%)
Frame = +3
Query: 36 ILNGVSFKIRHGEAVGIIGPSGTGKSTILKIIAGLLAPDKGEVYIRGRKRDGLISDDEIS 95
IL VSF R E V ++GPSGTGKST+L+IIAG + + + S ++
Sbjct: 54 ILKSVSFVARSSEIVAVVGPSGTGKSTLLRIIAGRVKDRDFDPKTISINDHPMTSPAQLR 233
Query: 96 GLRIGLVFQSAALFDSLTVRENVGF-LLYEHSSMPEEEISELVKETLAAVGLKGVENRLP 154
+ G V Q L LTV+E + F + M + V+ + +GL V +
Sbjct: 234 KI-CGFVAQEDNLLPLLTVKETLLFSAKFRLKEMTPNDREMRVESLMQELGLFHVSDSFV 410
Query: 155 SE-----LSGGMKKRVALARSIIFDTTKDSIEPEVLLYDEPTAGL 194
+ +SGG +KRV++ +I + P +LL DEPT+GL
Sbjct: 411 GDEENRGISGGERKRVSIGVDMIHN-------PPILLLDEPTSGL 524
>AV776921
Length = 435
Score = 63.2 bits (152), Expect = 5e-11
Identities = 48/147 (32%), Positives = 77/147 (51%), Gaps = 9/147 (6%)
Frame = +2
Query: 28 YKSFGEKKILNGVSFKIRHGEAVGIIGPSGTGKSTILKIIAGLLAPDKGEVYIRGRKRDG 87
Y S + +ILN + I G+ V ++G SG+GKST++ +I P G++ + G
Sbjct: 17 YPSRPDVEILNKLCLDIPSGKIVALVGGSGSGKSTVISLIERFYEPLSGDILLDGND--- 187
Query: 88 LISDDEISGLR--IGLVFQSAALFDSLTVRENVGFLLYEHSSMPEEEISELVKETLAAVG 145
I D ++ LR IGLV Q ALF + +++EN+ LY + EE+ VK + A
Sbjct: 188 -IRDLDLKWLRQQIGLVNQEPALF-ATSIKENI---LYGKDNATLEELKRAVKLSDAQSF 352
Query: 146 LKGVENRLPS-------ELSGGMKKRV 165
+ + RL + +LSGG K+R+
Sbjct: 353 INNLPERLETQVGERGIQLSGGQKQRI 433
>TC10604 similar to AAQ89671 (AAQ89671) At4g33460, partial (36%)
Length = 498
Score = 55.5 bits (132), Expect = 1e-08
Identities = 40/113 (35%), Positives = 59/113 (51%), Gaps = 1/113 (0%)
Frame = +2
Query: 35 KILNGVSFKIRHGEAVGIIGPSGTGKSTILKIIAGLLAPDKGEVYIRGRKRDGLISDDEI 94
++L S +I G+ ++GP+G GKST+LKI+AGLLAP G VY+ K
Sbjct: 197 RVLRDCSLRIPSGQFWMLLGPNGCGKSTLLKILAGLLAPTSGTVYVNEPK---------- 346
Query: 95 SGLRIGLVFQSAALFDSL-TVRENVGFLLYEHSSMPEEEISELVKETLAAVGL 146
VFQ+ + TV +V F L + ++P +E+ V+ L AVGL
Sbjct: 347 -----SFVFQNPDHQVVMPTVDADVAFGLGKF-NLPNDEVRSKVQRALHAVGL 487
>TC11559 similar to UP|Q9SXT3 (Q9SXT3) Multidrug resistance protein
(Fragment), partial (41%)
Length = 460
Score = 54.7 bits (130), Expect = 2e-08
Identities = 34/87 (39%), Positives = 50/87 (57%), Gaps = 2/87 (2%)
Frame = +3
Query: 36 ILNGVSFKIRHGEAVGIIGPSGTGKSTILKIIAGLLAPDKGEVYIRGRKRDGLISDDEIS 95
IL G+ I G VG+IGPSG+GKST+L+ + L P V++ R I ++
Sbjct: 135 ILKGIHLDIPKGVIVGVIGPSGSGKSTLLRALNRLWEPPSASVFLDARD----ICHLDVL 302
Query: 96 GLR--IGLVFQSAALFDSLTVRENVGF 120
LR +G++FQ ALF+ TV +NV +
Sbjct: 303 SLRRKVGMLFQLPALFEG-TVADNVRY 380
>TC9999 similar to UP|Q9FMU0 (Q9FMU0) Similarity to ABC transporter,
partial (43%)
Length = 656
Score = 53.1 bits (126), Expect = 5e-08
Identities = 33/86 (38%), Positives = 52/86 (60%), Gaps = 4/86 (4%)
Frame = +3
Query: 23 ECRDV-YKSFGEK-KILNGVSFKIRHGEAVGIIGPSGTGKSTILKIIAGLLAPDKGEVYI 80
E RDV Y++ G + K+LN V+F + I G SG+GK+T+L+++AG+ P G +YI
Sbjct: 270 EVRDVSYQAPGTQLKLLNSVTFSLPEKSFGLIFGQSGSGKTTLLQLLAGINKPTSGSIYI 449
Query: 81 RGRKRDGLISDDE--ISGLRIGLVFQ 104
+ DG S + + R+G+VFQ
Sbjct: 450 QNYGDDGSPSQSQEPLVPERVGIVFQ 527
>TC11425 similar to UP|Q9M1Q9 (Q9M1Q9) P-glycoprotein-like proetin, partial
(9%)
Length = 712
Score = 50.4 bits (119), Expect = 3e-07
Identities = 37/99 (37%), Positives = 52/99 (52%)
Frame = +2
Query: 157 LSGGMKKRVALARSIIFDTTKDSIEPEVLLYDEPTAGLDPIASTVVEDLIRSVHIKGRDA 216
LSGG K+RVA+AR+II P +LL DE T+ LD + VV+D + V +
Sbjct: 107 LSGGQKQRVAIARAII-------KTPNILLLDEATSALDAESERVVQDALDKVMVN---- 253
Query: 217 LGKPGNISSYVVVTHQHSTIKRAIDRLLFLHKGKLVWEG 255
+ V+V H+ STIK A D + L G +V +G
Sbjct: 254 -------RTTVIVAHRLSTIKNA-DVITVLKNGVIVEKG 346
>TC18135 homologue to UP|Q9MAY4 (Q9MAY4) ABC transporter homolog, partial
(50%)
Length = 968
Score = 49.3 bits (116), Expect = 7e-07
Identities = 53/208 (25%), Positives = 91/208 (43%), Gaps = 13/208 (6%)
Frame = +3
Query: 14 HEDDSDVLIECRDVYKSFGEKKILNGVSFKIRHGEAVGIIGPSGTGKSTILKIIAG--LL 71
H D+ IE V +F ++ ++ +G G++G +G GKST+L I L
Sbjct: 282 HPLSRDIRIESLSV--TFHGHDLIVDSELELNYGRRYGLLGLNGCGKSTLLTAIGHRELP 455
Query: 72 APDKGEVYIRGRKRDGLISDDEISGLRIGLVFQSAAL-----FDSLTVRENVGFLLYEHS 126
P+ ++Y R+ I ++S L + L ++L +++ G E
Sbjct: 456 IPEHMDIYHLTRE----IEASDMSALEAVVSCDEERLRLEKEAETLAAQDDGGGETLERI 623
Query: 127 SMPEEEISELVKETLAAVGLKGV------ENRLPSELSGGMKKRVALARSIIFDTTKDSI 180
E + E AA L G+ + + + SGG + R+ALAR++ I
Sbjct: 624 YERLEALDASTAEKRAAEILHGLGFDKKMQAKKTRDFSGGWRMRIALARALF-------I 782
Query: 181 EPEVLLYDEPTAGLDPIASTVVEDLIRS 208
P +LL DEPT LD A +E+ +++
Sbjct: 783 NPTILLLDEPTNHLDLEACVWLEESLKN 866
>BG662257
Length = 336
Score = 48.9 bits (115), Expect = 1e-06
Identities = 37/107 (34%), Positives = 55/107 (50%)
Frame = +1
Query: 133 ISELVKETLAAVGLKGVENRLPSELSGGMKKRVALARSIIFDTTKDSIEPEVLLYDEPTA 192
IS L + VG +G++ LSGG K+RVA+AR+I+ P++LL DE T+
Sbjct: 85 ISSLQQGYDTVVGDRGIQ------LSGGQKQRVAIARAIV-------KSPKILLLDEATS 225
Query: 193 GLDPIASTVVEDLIRSVHIKGRDALGKPGNISSYVVVTHQHSTIKRA 239
LD + VV+D + V + + +VV H+ STIK A
Sbjct: 226 ALDAESEKVVQDALDRVRVD-----------RTTIVVAHRLSTIKGA 333
>AV767093
Length = 603
Score = 47.8 bits (112), Expect = 2e-06
Identities = 17/47 (36%), Positives = 34/47 (72%)
Frame = +1
Query: 36 ILNGVSFKIRHGEAVGIIGPSGTGKSTILKIIAGLLAPDKGEVYIRG 82
+L G+S ++ GE +G++G +G+GKST+++++ L+ P G++ I G
Sbjct: 433 VLKGISLTVQGGEKIGVVGRTGSGKSTLIQVLFRLIEPSAGKIIIDG 573
>CB827630
Length = 405
Score = 46.2 bits (108), Expect = 6e-06
Identities = 38/129 (29%), Positives = 61/129 (46%), Gaps = 2/129 (1%)
Frame = +2
Query: 133 ISELVKETLAAVGL--KGVENRLPSELSGGMKKRVALARSIIFDTTKDSIEPEVLLYDEP 190
+++ V+E+L ++ L GV ++ + SGGMK+R+ +A S+I D P V+ DEP
Sbjct: 59 LTQAVEESLKSLNLFHGGVADKQAGKYSGGMKRRLIVAISLIGD-------PRVVYMDEP 217
Query: 191 TAGLDPIASTVVEDLIRSVHIKGRDALGKPGNISSYVVVTHQHSTIKRAIDRLLFLHKGK 250
+ GLDP + + L V + RD + ++ TH + DRL G
Sbjct: 218 STGLDPASR---KSLWNVVKLAKRD--------RAIILTTHSMEEAEALCDRLGIXVNGS 364
Query: 251 LVWEGMTHE 259
L G E
Sbjct: 365 LQCVGNAKE 391
>TC18647 similar to UP|Q9ASR9 (Q9ASR9) At2g01320/F10A8.20, partial (17%)
Length = 430
Score = 42.0 bits (97), Expect = 1e-04
Identities = 32/110 (29%), Positives = 50/110 (45%), Gaps = 11/110 (10%)
Frame = +1
Query: 20 VLIECRDVYKSFGEKK------ILNGVSFKIRHGEAVGIIGPSGTGKSTILKIIAGLLAP 73
V I ++ SF +K +L VS + + G + I+GPSG+GK+T+L ++AG LA
Sbjct: 40 VTIRWSNINCSFSDKSSKSVRFLLKNVSGEAKPGRLLAIMGPSGSGKTTLLNVLAGQLAA 219
Query: 74 D-----KGEVYIRGRKRDGLISDDEISGLRIGLVFQSAALFDSLTVRENV 118
G + G+ + + V Q F LTVRE +
Sbjct: 220 SPRLHLSGLLEFNGK-------PGSKNAYKFAYVRQEDLFFSQLTVRETL 348
>TC15722 similar to UP|Q9FMU0 (Q9FMU0) Similarity to ABC transporter,
partial (37%)
Length = 624
Score = 42.0 bits (97), Expect = 1e-04
Identities = 28/70 (40%), Positives = 41/70 (58%), Gaps = 1/70 (1%)
Frame = +3
Query: 144 VGLKGVE-NRLPSELSGGMKKRVALARSIIFDTTKDSIEPEVLLYDEPTAGLDPIASTVV 202
VGL G+ ++ P LSGG K+R+ALA ++ P++L+ DEP AGLD A V
Sbjct: 27 VGLSGISLDKNPQSLSGGYKRRLALAIQLVQ-------VPDLLILDEPLAGLDWKARADV 185
Query: 203 EDLIRSVHIK 212
L++ H+K
Sbjct: 186 VKLLK--HLK 209
>AV416268
Length = 424
Score = 41.6 bits (96), Expect = 2e-04
Identities = 46/151 (30%), Positives = 65/151 (42%), Gaps = 8/151 (5%)
Frame = +3
Query: 111 SLTVRENVGFLLYEH--SSMPEEEISELVKETLAAVGLKGVENRL-----PSELSGGMKK 163
+LTV E V + + SM + E E T+ +GL+ N +SGG K+
Sbjct: 9 TLTVGEAVYYSAHLQLPDSMSKSEKRERADFTIKEMGLQDAINTRIGGWGSKGVSGGQKR 188
Query: 164 RVALARSIIFDTTKDSIEPEVLLYDEPTAGLDPIASTVVEDLIRSVHIKGRDALGKPGNI 223
RV++ I+ P +L DEPT+GLD AS V I S++ K G
Sbjct: 189 RVSICIEIL-------THPRLLFLDEPTSGLDSAASYHVISRISSLN-------KKDGIQ 326
Query: 224 SSYVVVTHQHST-IKRAIDRLLFLHKGKLVW 253
+ V HQ S I + L L GK V+
Sbjct: 327 RTIVASIHQPSNEIFQLFHSLCLLSSGKTVY 419
>AV417384
Length = 380
Score = 38.9 bits (89), Expect = 0.001
Identities = 17/45 (37%), Positives = 29/45 (63%)
Frame = +2
Query: 34 KKILNGVSFKIRHGEAVGIIGPSGTGKSTILKIIAGLLAPDKGEV 78
++IL V+ + G A+ + G +G+GKST L+++AG P GE+
Sbjct: 239 QQILRHVNVSLHDGGALVLTGANGSGKSTFLRMLAGFSRPSAGEI 373
>TC13598 similar to UP|Q9M1Q9 (Q9M1Q9) P-glycoprotein-like proetin, partial
(7%)
Length = 552
Score = 36.2 bits (82), Expect = 0.006
Identities = 25/74 (33%), Positives = 38/74 (50%)
Frame = +3
Query: 182 PEVLLYDEPTAGLDPIASTVVEDLIRSVHIKGRDALGKPGNISSYVVVTHQHSTIKRAID 241
P +LL DE T+ LD + VV+D + V + + V+V H+ STIK A D
Sbjct: 12 PNILLLDEXTSALDVESERVVQDALDKVMVN-----------RTTVIVAHRLSTIKSA-D 155
Query: 242 RLLFLHKGKLVWEG 255
++ L G +V +G
Sbjct: 156 VIIVLKNGVIVEKG 197
>TC16623 weakly similar to UP|TAP2_HUMAN (Q03519) Antigen peptide
transporter 2 (APT2) (Peptide transporter TAP2) (Peptide
transporter PSF2) (Peptide supply factor 2) (PSF-2)
(Peptide transporter involved in antigen processing 2),
partial (4%)
Length = 530
Score = 35.8 bits (81), Expect = 0.008
Identities = 17/42 (40%), Positives = 26/42 (61%), Gaps = 1/42 (2%)
Frame = +1
Query: 29 KSFGEKK-ILNGVSFKIRHGEAVGIIGPSGTGKSTILKIIAG 69
K GE K +L G++ I GE ++G +G+GKST K++ G
Sbjct: 187 KIVGEHKDLLKGLNLTINRGEVHALMGANGSGKSTFAKVLVG 312
>TC9119 homologue to AAQ65167 (AAQ65167) At1g64550, partial (20%)
Length = 666
Score = 32.7 bits (73), Expect = 0.071
Identities = 38/135 (28%), Positives = 62/135 (45%), Gaps = 5/135 (3%)
Frame = +3
Query: 120 FLLYEHSSMPEEEISELVKETLAAVGLKGVENRLPSE----LSGGMKKRVALARSIIFDT 175
+++ + +PE+++ A +G GV L + LSGG K RVA A+ I F
Sbjct: 57 YMMRCYPGVPEQKLR-------AHLGSFGVTGNLALQPMYTLSGGQKSRVAFAK-ITFK- 209
Query: 176 TKDSIEPEVLLYDEPTAGLDPIASTVVEDLIRS-VHIKGRDALGKPGNISSYVVVTHQHS 234
+P ++L DEP+ LD A VE LI+ V +G ++V+H
Sbjct: 210 -----KPHIILLDEPSHHLDLDA---VEALIQGLVMFQG-----------GILMVSHDEH 332
Query: 235 TIKRAIDRLLFLHKG 249
I ++D L + +G
Sbjct: 333 LISGSVDELWVVSQG 377
>TC15900 similar to UP|Q8GU54 (Q8GU54) SMC3 protein, partial (15%)
Length = 881
Score = 31.6 bits (70), Expect = 0.16
Identities = 28/78 (35%), Positives = 38/78 (47%)
Frame = +1
Query: 130 EEEISELVKETLAAVGLKGVENRLPSELSGGMKKRVALARSIIFDTTKDSIEPEVLLYDE 189
E+ I VK + G E + +LSGG K VAL ++IF + P L+DE
Sbjct: 169 EKYIGVKVKVSFTGQG----ETQSMKQLSGGQKTVVAL--TLIFAIQRCDPAP-FYLFDE 327
Query: 190 PTAGLDPIASTVVEDLIR 207
A LDP T V ++IR
Sbjct: 328 IDAALDPQYRTAVGNMIR 381
>AU089197
Length = 658
Score = 30.0 bits (66), Expect = 0.46
Identities = 15/47 (31%), Positives = 27/47 (56%)
Frame = +1
Query: 36 ILNGVSFKIRHGEAVGIIGPSGTGKSTILKIIAGLLAPDKGEVYIRG 82
+L V+F + +GI +G+GKST+L+ + ++ P G + I G
Sbjct: 406 VLKDVTFIFSGQKKIGIXXRTGSGKSTLLQALF*IVXPLSGCILIDG 546
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.315 0.137 0.379
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,687,499
Number of Sequences: 28460
Number of extensions: 42210
Number of successful extensions: 170
Number of sequences better than 10.0: 50
Number of HSP's better than 10.0 without gapping: 167
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 167
length of query: 281
length of database: 4,897,600
effective HSP length: 89
effective length of query: 192
effective length of database: 2,364,660
effective search space: 454014720
effective search space used: 454014720
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 55 (25.8 bits)
Medicago: description of AC124218.7