BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC124217.5 + phase: 0 /pseudo
(1307 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP063694 82 4e-16
BP033602 64 2e-10
AV766088 63 3e-10
TC8302 50 3e-06
BP054557 48 9e-06
TC9039 47 2e-05
AV764934 41 0.001
TC18003 similar to PIR|T05112|T05112 splicing factor 9G8-like SR... 41 0.001
BP058111 41 0.001
TC17853 similar to UP|Q42412 (Q42412) RNA-binding protein RZ-1, ... 39 0.004
BP063902 38 0.009
TC11095 similar to PIR|T05112|T05112 splicing factor 9G8-like SR... 38 0.012
BP081717 37 0.015
BP073434 35 0.059
AV420911 35 0.077
BP065763 35 0.077
AV773507 34 0.17
TC18927 similar to PIR|AI2934|AI2934 chromate transport protein ... 33 0.22
TC9337 similar to UP|PME_PRUPE (Q43062) Pectinesterase PPE8B pre... 33 0.38
TC9435 similar to UP|Q9AXJ7 (Q9AXJ7) Phosphatase-like protein Mt... 27 0.83
>BP063694
Length = 511
Score = 82.4 bits (202), Expect = 4e-16
Identities = 48/130 (36%), Positives = 70/130 (52%), Gaps = 3/130 (2%)
Frame = -2
Query: 391 GLYIL--DGSIVIGNASVASVVPHNNSELWHLRLGHVSERGLVELAKQGLLGKDK-LDKL 447
GLY+L D ++ + +S +P + ELWH RLGHV+ + +L K G L L K
Sbjct: 435 GLYVLNQDSQALL---TTSSPLPRASFELWHSRLGHVNFDIIKQLHKHGCLDVSSILPKP 265
Query: 448 EFCEHCILGKQHRVKFGSGMHHSSRLFEYVHSDLLGPSKTPTHGGGSYFLSIIDDYSRRV 507
C C + K R+ F +S + + +H DL GPS + G SYF+ +DD+SR
Sbjct: 264 ICCTSCQMAKSKRLVFHDNNKRASAVLDLIHCDLRGPSPVASIDGFSYFVIFVDDFSRFT 85
Query: 508 WVFVLKKKSD 517
W + LK+KSD
Sbjct: 84 WFYPLKRKSD 55
>BP033602
Length = 533
Score = 63.9 bits (154), Expect = 2e-10
Identities = 36/88 (40%), Positives = 49/88 (54%)
Frame = +3
Query: 417 LWHLRLGHVSERGLVELAKQGLLGKDKLDKLEFCEHCILGKQHRVKFGSGMHHSSRLFEY 476
LWH RLGH + + L L L LE CE C+L K R + S +H+SR F
Sbjct: 276 LWHNRLGHPNFQYLRHLFPD-LFKNVNCSSLE-CESCVLAKNQRAPYYSQPYHASRPFYL 449
Query: 477 VHSDLLGPSKTPTHGGGSYFLSIIDDYS 504
+HSD+ GPSK T G +F++ IDD++
Sbjct: 450 IHSDVWGPSKITTQFGKRWFVTFIDDHT 533
>AV766088
Length = 501
Score = 62.8 bits (151), Expect = 3e-10
Identities = 34/103 (33%), Positives = 51/103 (49%)
Frame = -3
Query: 417 LWHLRLGHVSERGLVELAKQGLLGKDKLDKLEFCEHCILGKQHRVKFGSGMHHSSRLFEY 476
LWH RLGH S L L + + L+ CE C+ GK R+ F S + + F+
Sbjct: 307 LWHSRLGHPSSSALRYLRSNKFISYELLNYSPVCESCVFGKHVRLPFVSSNNVTVMPFDI 128
Query: 477 VHSDLLGPSKTPTHGGGSYFLSIIDDYSRRVWVFVLKKKSDTF 519
+HSD L S + G +++ +DD++ +W F L KS F
Sbjct: 127 LHSD-LWTSPVLSSAGHRFYVLFLDDFTDFLWTFPLSNKSQVF 2
>TC8302
Length = 494
Score = 49.7 bits (117), Expect = 3e-06
Identities = 40/162 (24%), Positives = 73/162 (44%), Gaps = 2/162 (1%)
Frame = +3
Query: 105 QQLYSFKMVESISISEQLTEFNKILVDLANIEVNTEDEDKALLLLCSLPKSFEHFKDTIL 164
++LY+ +M ES S+S+ + N + L+ + ++A LLL SLP S+ D ++
Sbjct: 3 KRLYTLRMSESTSMSDHIDHMNTLFAQLSASNFTIGENERAELLLESLPDSY----DQLV 170
Query: 165 YGKEGTTTLEEVQAALRTKELTKFKDLKVDEGSEGLNVARGRNEHRGKGKGKSRSKSRSK 224
+ + + L ++ ++E S+ N R + + + ++ RSK
Sbjct: 171 INIKNNNIVNHL-------PLMMLSEVLLEEDSQR*N-KEDR*DSSKPMEALTMTRGRSK 326
Query: 225 GFDKSKYKCFLCHKQGHFKKDC--PDKGGDGSPSVQVAEASN 264
K KC+ C ++ H KKDC K D +P + SN
Sbjct: 327 SRKKINLKCYHCGQR*HLKKDCWFNKKSSDVTPRFRWRHFSN 452
>BP054557
Length = 538
Score = 48.1 bits (113), Expect = 9e-06
Identities = 41/163 (25%), Positives = 74/163 (45%), Gaps = 13/163 (7%)
Frame = -2
Query: 286 VLDSGCSYHMCPRKEYFETLTLKEGGVVRLGNNKACKVQGMGNVRLKMFDGREFLLRDVR 345
+LD+G ++H+C + F+ L ++ + N + + + ++ ++ +F L V
Sbjct: 537 LLDTGATHHVCHNLDVFQALRQVHPVLLSMPNGQ----KEIAHMADSVYFSEKFFLAXVL 370
Query: 346 FVPELKRNLISLSMFDGLGYCTRIEHGVCKI--SHGALITVKGSK-MNGLY-ILDGSIVI 401
+VP NLIS+S C + C + L T+ ++ GLY ++D ++
Sbjct: 369 YVPSFNFNLISVSALVKSLNCRLVIFDECCLLQERPTLKTIGAAEERGGLYAVIDPAVKA 190
Query: 402 ----GNASVASVVPHNN-----SELWHLRLGHVSERGLVELAK 435
++ + V H N LWHLRLGH S + L L K
Sbjct: 189 KHPPAEGNIKTQVSHFNFCQFDCNLWHLRLGHTSSKKLAILQK 61
>TC9039
Length = 1218
Score = 47.0 bits (110), Expect = 2e-05
Identities = 41/168 (24%), Positives = 66/168 (38%), Gaps = 7/168 (4%)
Frame = +2
Query: 86 AKLESLYMTKSLAHRQLLKQQLYSFKMVESISISEQLTEFNKILVDLANIEVNTEDEDKA 145
A++E + A L Q L S K +I E + + I L +++ D+
Sbjct: 410 AEIEKRFAKSDKAETSTLLQNLISMKYQGKGNIREYIMGMSNIASKLKALKLELSDDLLI 589
Query: 146 LLLLCSLPKSFEHFKDTILYGKEGTTTLEEVQAALRTKELTKFKDLKVDEGSEGLNVARG 205
L+L SLP F FK + KE + E + ++ +E K E A
Sbjct: 590 HLVLLSLPAQFSQFKISYNCPKEKWSLNELISFCVQEEERLK---------QERKESAHF 742
Query: 206 RNEHRGKGKGKSRSKSRSKGFD-------KSKYKCFLCHKQGHFKKDC 246
+ + KGK K + +++ D K C+ C+ GH KK C
Sbjct: 743 VSTSKDKGKRKKTVEPKNEAADAPAPKKQKEDDTCYFCNVSGHMKKKC 886
Score = 44.7 bits (104), Expect = 1e-04
Identities = 27/85 (31%), Positives = 45/85 (52%), Gaps = 2/85 (2%)
Frame = +3
Query: 313 VRLGNNKACKVQGMGNVRLKMFDGREFLLRDVRFVPELKRNLISLSMFDGLGYCTRIEHG 372
+ +G+ K +V+ +G+ RL + G L+D VP +RNLIS+S D GY +
Sbjct: 960 IYVGDGKTVEVEAIGHFRLLLCTGFYLDLKDTFVVPSFRRNLISVSNLDKSGYSCSFGNS 1139
Query: 373 VCKISHGALITVKGSKM--NGLYIL 395
++S + I GS + + LY+L
Sbjct: 1140 KAELSFNSNIVGTGSLIGYDNLYLL 1214
>AV764934
Length = 406
Score = 40.8 bits (94), Expect = 0.001
Identities = 26/69 (37%), Positives = 35/69 (50%), Gaps = 1/69 (1%)
Frame = -2
Query: 418 WHLRLGHVSERGLVELAKQGLLGKDKLDKLEFCEHCILGKQHRVKFGSGMHHSSRL-FEY 476
WH RLGH L + + D +EF C LGK HR+ S M ++ L FE
Sbjct: 213 WHSRLGHPHLDNLKRVLSSCNVSYSPKDTVEFRTACCLGKAHRLP--SQMSTTTYLPFEL 40
Query: 477 VHSDLLGPS 485
++SDL GP+
Sbjct: 39 IYSDLWGPA 13
>TC18003 similar to PIR|T05112|T05112 splicing factor 9G8-like SR protein
RSZp22 [validated] - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (42%)
Length = 587
Score = 40.8 bits (94), Expect = 0.001
Identities = 21/71 (29%), Positives = 33/71 (45%)
Frame = +1
Query: 181 RTKELTKFKDLKVDEGSEGLNVARGRNEHRGKGKGKSRSKSRSKGFDKSKYKCFLCHKQG 240
R +E + ++ +G N R H KG G R R +G + KC+ C + G
Sbjct: 16 RERERERERERDAIHALDGKNGWRVELSHNSKGGGGGRGGGRGRGGED--LKCYECGEPG 189
Query: 241 HFKKDCPDKGG 251
HF ++C +GG
Sbjct: 190 HFARECRSRGG 222
>BP058111
Length = 570
Score = 40.8 bits (94), Expect = 0.001
Identities = 22/64 (34%), Positives = 35/64 (54%), Gaps = 1/64 (1%)
Frame = -1
Query: 417 LWHLRLGHVSERGLVELAKQ-GLLGKDKLDKLEFCEHCILGKQHRVKFGSGMHHSSRLFE 475
LWHLRLGH S + L L K + K+ C+ C + KQ ++ F + + SS +F+
Sbjct: 186 LWHLRLGHTSSKKLAILQKNFPFITCKKITSP--CDTCHMAKQKKLSFPNSVTLSSEIFD 13
Query: 476 YVHS 479
+H+
Sbjct: 12 LIHT 1
>TC17853 similar to UP|Q42412 (Q42412) RNA-binding protein RZ-1, partial
(58%)
Length = 881
Score = 39.3 bits (90), Expect = 0.004
Identities = 17/49 (34%), Positives = 27/49 (54%), Gaps = 4/49 (8%)
Frame = +2
Query: 206 RNEHRGKGKGKSRSKSRSKGFDKSKY----KCFLCHKQGHFKKDCPDKG 250
R RG+ + + RS+G+ S+ +CF C K GHF ++CP +G
Sbjct: 326 RYRERGRDRDDRGDRDRSRGYGGSRGSNGGECFKCGKPGHFARECPSEG 472
>BP063902
Length = 494
Score = 38.1 bits (87), Expect = 0.009
Identities = 18/51 (35%), Positives = 25/51 (48%)
Frame = -3
Query: 415 SELWHLRLGHVSERGLVELAKQGLLGKDKLDKLEFCEHCILGKQHRVKFGS 465
S +WH RLGH + L L L+ + C+ C+LGK R+ F S
Sbjct: 189 SSVWHHRLGHPASSALNHLRNNKLIFCEPSRSSSVCDSCVLGKHVRLPFSS 37
>TC11095 similar to PIR|T05112|T05112 splicing factor 9G8-like SR protein
RSZp22 [validated] - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (89%)
Length = 912
Score = 37.7 bits (86), Expect = 0.012
Identities = 19/51 (37%), Positives = 26/51 (50%)
Frame = +1
Query: 203 ARGRNEHRGKGKGKSRSKSRSKGFDKSKYKCFLCHKQGHFKKDCPDKGGDG 253
+RG G G+G RS G D KC+ C + GHF ++C +GG G
Sbjct: 298 SRGGGGGGGGGRGGGRSGGGGGGSDM---KCYECGEPGHFARECRMRGGSG 441
>BP081717
Length = 291
Score = 37.4 bits (85), Expect = 0.015
Identities = 25/85 (29%), Positives = 40/85 (46%), Gaps = 5/85 (5%)
Frame = -1
Query: 384 VKGSKMNGLYILDGSIVIGNASVASVVPHNNS-----ELWHLRLGHVSERGLVELAKQGL 438
++ K + L L G I ++A VP N ++WH RLGH S + L ++K +
Sbjct: 282 LRNLKESVLRSLIGISQISFVNIAETVPGKNGSHPQFDMWHFRLGHPSSKVLQHISK--V 109
Query: 439 LGKDKLDKLEFCEHCILGKQHRVKF 463
KL C+ C + KQ ++ F
Sbjct: 108 FPYVKLHSNIICDFCHMAKQTKLPF 34
>BP073434
Length = 490
Score = 35.4 bits (80), Expect = 0.059
Identities = 16/47 (34%), Positives = 22/47 (46%)
Frame = -1
Query: 413 NNSELWHLRLGHVSERGLVELAKQGLLGKDKLDKLEFCEHCILGKQH 459
N +WH RLGH + L + + + D +FC C LGK H
Sbjct: 187 NKFLIWHSRLGHPHQDALQAVLQSCNIHLSSRDMTDFCYACCLGKSH 47
>AV420911
Length = 418
Score = 35.0 bits (79), Expect = 0.077
Identities = 17/49 (34%), Positives = 24/49 (48%)
Frame = +2
Query: 198 EGLNVARGRNEHRGKGKGKSRSKSRSKGFDKSKYKCFLCHKQGHFKKDC 246
+G N R H KG G R R +G + KC+ C + GHF ++C
Sbjct: 275 DGKNGWRVELSHNSKGGGGGRGGGRGRGGED--LKCYECGEPGHFAREC 415
>BP065763
Length = 537
Score = 35.0 bits (79), Expect = 0.077
Identities = 21/69 (30%), Positives = 31/69 (44%), Gaps = 4/69 (5%)
Frame = -1
Query: 185 LTKFKD----LKVDEGSEGLNVARGRNEHRGKGKGKSRSKSRSKGFDKSKYKCFLCHKQG 240
L K KD L +G+ GR E + K ++ +G ++ C+ C K G
Sbjct: 522 LKKEKDHSIHLTTHDGASXSKAKPGRKEQK-----KDQALKVKEGRIHKEHVCYFCKKAG 358
Query: 241 HFKKDCPDK 249
HFKK CP +
Sbjct: 357 HFKKYCPKR 331
Score = 30.0 bits (66), Expect = 2.5
Identities = 21/80 (26%), Positives = 34/80 (42%), Gaps = 1/80 (1%)
Frame = -2
Query: 315 LGNNKACKVQGMGNVRLKMFDGREFLLRDVRFVPELKRNLISLSMFDGLG-YCTRIEHGV 373
+GN +++G+G RL + G + L +V RNL+S I HG
Sbjct: 260 MGNQMKARIEGIGTYRLILDTGHQLDLEKCLYVRGCARNLVSSRKVGSFRVLILXIGHGG 81
Query: 374 CKISHGALITVKGSKMNGLY 393
+ A G+ ++GLY
Sbjct: 80 FSLYKLAYYYGSGTLLDGLY 21
>AV773507
Length = 496
Score = 33.9 bits (76), Expect = 0.17
Identities = 19/64 (29%), Positives = 25/64 (38%)
Frame = +2
Query: 209 HRGKGKGKSRSKSRSKGFDKSKYKCFLCHKQGHFKKDCPDKGGDGSPSVQVAEASNEEGY 268
+RG G S G + CF C + GH +DCP GG G S+ Y
Sbjct: 278 YRGGGYSSGGRGSYGAGDRVGQDDCFECGRPGHRARDCPLAGGGGGRGRGGGSLSSRPRY 457
Query: 269 ESTG 272
+G
Sbjct: 458 GGSG 469
>TC18927 similar to PIR|AI2934|AI2934 chromate transport protein chrA
[imported] - Agrobacterium tumefaciens
(strain C58, Dupont) {Agrobacterium tumefaciens;},
partial (6%)
Length = 561
Score = 33.5 bits (75), Expect = 0.22
Identities = 18/47 (38%), Positives = 22/47 (46%), Gaps = 4/47 (8%)
Frame = -2
Query: 206 RNEHRGKGKGKSRSKS----RSKGFDKSKYKCFLCHKQGHFKKDCPD 248
R ++RG G S S R D S+ C C K+GHF CPD
Sbjct: 422 RPQNRGTSSGYSHSFGNFVPRPTQSDTSEIVCHRCSKKGHFANRCPD 282
>TC9337 similar to UP|PME_PRUPE (Q43062) Pectinesterase PPE8B precursor
(Pectin methylesterase) (PE) , partial (54%)
Length = 1145
Score = 32.7 bits (73), Expect = 0.38
Identities = 14/32 (43%), Positives = 20/32 (61%)
Frame = +3
Query: 450 CEHCILGKQHRVKFGSGMHHSSRLFEYVHSDL 481
C+ C+LGK R+ F S + R F+ +HSDL
Sbjct: 1029 CDSCVLGKHVRLPFSSSETITLRPFDILHSDL 1124
>TC9435 similar to UP|Q9AXJ7 (Q9AXJ7) Phosphatase-like protein Mtc923,
partial (17%)
Length = 486
Score = 27.3 bits (59), Expect(2) = 0.83
Identities = 14/36 (38%), Positives = 18/36 (49%), Gaps = 2/36 (5%)
Frame = -2
Query: 199 GLNVARGRNEHRGKGKGKSR--SKSRSKGFDKSKYK 232
G+ RGR GKGK R K RS+G + + K
Sbjct: 341 GIGARRGRERGSGKGKASVRETGKGRSQGIEARRVK 234
Score = 22.7 bits (47), Expect(2) = 0.83
Identities = 8/20 (40%), Positives = 12/20 (60%)
Frame = -3
Query: 248 DKGGDGSPSVQVAEASNEEG 267
++GGDG V++ EEG
Sbjct: 145 EEGGDGGVEVEIVAFEEEEG 86
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.346 0.154 0.537
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 23,681,900
Number of Sequences: 28460
Number of extensions: 350686
Number of successful extensions: 2885
Number of sequences better than 10.0: 54
Number of HSP's better than 10.0 without gapping: 2766
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2852
length of query: 1307
length of database: 4,897,600
effective HSP length: 101
effective length of query: 1206
effective length of database: 2,023,140
effective search space: 2439906840
effective search space used: 2439906840
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 38 (21.7 bits)
S2: 61 (28.1 bits)
Medicago: description of AC124217.5