BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC124215.9 - phase: 0
(475 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BI417505 289 6e-79
BI418609 228 2e-60
TC17951 similar to UP|Q9ZVI5 (Q9ZVI5) At2g38630 protein, partial... 84 5e-22
TC10112 similar to UP|Q9ZVI5 (Q9ZVI5) At2g38630 protein, partial... 74 7e-14
TC12074 70 7e-13
TC13198 33 0.078
TC9606 homologue to UP|AAQ05907 (AAQ05907) Cytochrome b6-f compl... 30 0.86
TC14483 29 1.9
BP068601 28 2.5
TC14354 homologue to UP|Q945R0 (Q945R0) Plastidic aldolase (Frag... 28 3.3
TC8217 similar to UP|Q8H6S5 (Q8H6S5) CTV.2, partial (8%) 28 4.3
TC8384 similar to UP|P93698 (P93698) CPRD46 protein , partial (48%) 28 4.3
TC14353 homologue to UP|Q8LL68 (Q8LL68) Latex plastidic aldolase... 28 4.3
TC11148 homologue to UP|Q7X9B8 (Q7X9B8) Ferrous ion membrane tra... 27 5.6
TC15179 similar to PIR|A31676|A31676 brain natriuretic factor pr... 27 5.6
BP063972 27 7.3
TC11336 similar to UP|Q8S9A5 (Q8S9A5) Glucosyltransferase like p... 27 7.3
TC15231 27 9.5
TC10830 weakly similar to UP|Q94JL9 (Q94JL9) At1g68400/T2E12_5, ... 27 9.5
>BI417505
Length = 483
Score = 289 bits (740), Expect = 6e-79
Identities = 139/161 (86%), Positives = 155/161 (95%)
Frame = +1
Query: 119 SRETNERICFLNICPDEVIRSLFYNKNNDSLITVSVYASENFSSLKCRSTRIEYIKRAKP 178
SRE+N+RICFLN+ PDEVIRSLFYNKNNDSLITVSVYAS+++SSLKCRSTRIEYI+R KP
Sbjct: 1 SRESNQRICFLNVSPDEVIRSLFYNKNNDSLITVSVYASDSYSSLKCRSTRIEYIRRGKP 180
Query: 179 DAGFPLFQSESLKWPGFVEFDDVNAKVLTYSAQDSIYKVFDLKNYTLLYSISDRNVQEIK 238
DAGF LF+SESLKWPGFVEFDDVN KVLT+SAQDSIYKVFDLKNYT+LYSISD+NVQEIK
Sbjct: 181 DAGFALFESESLKWPGFVEFDDVNGKVLTFSAQDSIYKVFDLKNYTMLYSISDKNVQEIK 360
Query: 239 ISPGIMLLIFNRASGHIPLKIISIEDGTVLKAFNHLLHRNK 279
ISPGIMLLIF +AS H+PLKI+SIEDGTVLK+FNHLL+RNK
Sbjct: 361 ISPGIMLLIFAKASSHVPLKILSIEDGTVLKSFNHLLYRNK 483
>BI418609
Length = 538
Score = 228 bits (581), Expect = 2e-60
Identities = 118/163 (72%), Positives = 133/163 (81%), Gaps = 5/163 (3%)
Frame = +3
Query: 1 MDERRRGVWSCRRIVATK----GESSNNGSKPNIKINNKN-IIKKLQTREISPKPHRSFV 55
M+ RRRG W RRIVAT+ G + +NG + N +N + ++KKL REI KPHR+F
Sbjct: 48 MEGRRRGGWCGRRIVATRRGTSGATVSNGFRLNSTTSNDDSVVKKLFGREICSKPHRAFA 227
Query: 56 AATSPHRFQNMRLTHQFDTHDPKHHSSPSPFLPFLMKRTKVVEIVAAKNIVFALAHSGLC 115
A T+ HRF+ MRLT Q+DTHDPK SSPSP LPFLMKRTKVVEIVAAK+IVFALAHSGLC
Sbjct: 228 ATTASHRFRTMRLTQQYDTHDPKGPSSPSPLLPFLMKRTKVVEIVAAKDIVFALAHSGLC 407
Query: 116 AAFSRETNERICFLNICPDEVIRSLFYNKNNDSLITVSVYASE 158
AAFSRETNERIC LN+CPDEVIRSLFYNKNNDSLITVSVYASE
Sbjct: 408 AAFSRETNERICLLNVCPDEVIRSLFYNKNNDSLITVSVYASE 536
>TC17951 similar to UP|Q9ZVI5 (Q9ZVI5) At2g38630 protein, partial (13%)
Length = 586
Score = 84.3 bits (207), Expect(2) = 5e-22
Identities = 42/56 (75%), Positives = 46/56 (82%), Gaps = 3/56 (5%)
Frame = +3
Query: 423 DCSSTCSC---KHTDSSQLRSSVAEALEDITALFYDEDRNEIYTGNRHGFVHVWSN 475
+ SS SC K +SQ+RS+VAEALEDITALFYDEDRNEIYTGNRHG VHVWSN
Sbjct: 111 ETSSGNSCNSRKRKHASQIRSTVAEALEDITALFYDEDRNEIYTGNRHGLVHVWSN 278
Score = 37.0 bits (84), Expect(2) = 5e-22
Identities = 16/27 (59%), Positives = 21/27 (77%)
Frame = +2
Query: 392 EANAGSINVSNILTGKCVAKINAANCI 418
E NAGSI VS + TGKC+A+I A+N +
Sbjct: 2 EGNAGSIYVSTLFTGKCLAQIRASNTL 82
>TC10112 similar to UP|Q9ZVI5 (Q9ZVI5) At2g38630 protein, partial (8%)
Length = 622
Score = 73.6 bits (179), Expect = 7e-14
Identities = 36/51 (70%), Positives = 42/51 (81%)
Frame = +3
Query: 425 SSTCSCKHTDSSQLRSSVAEALEDITALFYDEDRNEIYTGNRHGFVHVWSN 475
+S+ S K S++RS+VAEALEDI ALFYDE+ NEIYTGNRHG VHVWSN
Sbjct: 246 NSSNSRK*KHVSKIRSTVAEALEDIIALFYDEECNEIYTGNRHGLVHVWSN 398
>TC12074
Length = 490
Score = 70.1 bits (170), Expect = 7e-13
Identities = 39/88 (44%), Positives = 53/88 (59%)
Frame = +2
Query: 12 RRIVATKGESSNNGSKPNIKINNKNIIKKLQTREISPKPHRSFVAATSPHRFQNMRLTHQ 71
RR+VA KG + N +KKLQ REIS K R+F + RF+N+RL +
Sbjct: 245 RRVVAKKGPRRGGADDGFV-----NSVKKLQRREISSKRDRAFTMTDAQERFRNIRLQEE 409
Query: 72 FDTHDPKHHSSPSPFLPFLMKRTKVVEI 99
+DTHDPK SS + LPFL +R+K++EI
Sbjct: 410 YDTHDPKGPSS-AVVLPFLRRRSKIIEI 490
>TC13198
Length = 507
Score = 33.5 bits (75), Expect = 0.078
Identities = 12/14 (85%), Positives = 13/14 (92%)
Frame = +3
Query: 462 YTGNRHGFVHVWSN 475
YTGNRHG VHVWS+
Sbjct: 3 YTGNRHGLVHVWSH 44
>TC9606 homologue to UP|AAQ05907 (AAQ05907) Cytochrome b6-f complex subunit
4 (Fragment), partial (9%)
Length = 617
Score = 30.0 bits (66), Expect = 0.86
Identities = 21/65 (32%), Positives = 30/65 (45%), Gaps = 1/65 (1%)
Frame = -3
Query: 29 NIKINNKNIIKKLQTREISPKPHRSFVAATSPHRFQNMRLTHQFDTHDPKHHSS-PSPFL 87
N+K N K IK + P+ H SFV A S Q + HQ ++ + + PS F
Sbjct: 471 NMKYNTKKTIKSI*KH*KKPQHHLSFVLANSV*D*QRFKECHQLPNYNLEEITCFPSQFF 292
Query: 88 PFLMK 92
FL +
Sbjct: 291 LFLSR 277
>TC14483
Length = 1158
Score = 28.9 bits (63), Expect = 1.9
Identities = 13/27 (48%), Positives = 18/27 (66%)
Frame = +3
Query: 338 NRTVSVWNFRGELVTSFEDHLLWHPDC 364
N TV V N +GEL +FE ++LW+ C
Sbjct: 918 NFTVLVPNRKGELKITFEAYILWNDRC 998
>BP068601
Length = 518
Score = 28.5 bits (62), Expect = 2.5
Identities = 13/27 (48%), Positives = 18/27 (66%)
Frame = -3
Query: 338 NRTVSVWNFRGELVTSFEDHLLWHPDC 364
N TV V N +GEL +FE ++LW+ C
Sbjct: 216 NPTVLVPNPQGELKITFEAYILWNDRC 136
>TC14354 homologue to UP|Q945R0 (Q945R0) Plastidic aldolase (Fragment),
partial (98%)
Length = 662
Score = 28.1 bits (61), Expect = 3.3
Identities = 11/34 (32%), Positives = 19/34 (55%)
Frame = +2
Query: 74 THDPKHHSSPSPFLPFLMKRTKVVEIVAAKNIVF 107
T P HH SPS +P LM ++ +++ + + F
Sbjct: 173 TLPPPHHPSPSELVPMLMSLLRLRKLLLHQGVAF 274
>TC8217 similar to UP|Q8H6S5 (Q8H6S5) CTV.2, partial (8%)
Length = 634
Score = 27.7 bits (60), Expect = 4.3
Identities = 13/44 (29%), Positives = 24/44 (54%)
Frame = +2
Query: 430 CKHTDSSQLRSSVAEALEDITALFYDEDRNEIYTGNRHGFVHVW 473
C+ S+ L SSV+ ++ + + ++ N+ TG G VHV+
Sbjct: 65 CRINPSAYLPSSVSSNVQPLVIAAHPQEPNQFATGLSDGTVHVF 196
>TC8384 similar to UP|P93698 (P93698) CPRD46 protein , partial (48%)
Length = 1660
Score = 27.7 bits (60), Expect = 4.3
Identities = 13/42 (30%), Positives = 21/42 (49%)
Frame = +2
Query: 336 FRNRTVSVWNFRGELVTSFEDHLLWHPDCNTNNIYITSDQDL 377
+ N + +W+ VT + +H P+ NT I SDQ+L
Sbjct: 617 YANDGLILWDAIKSWVTDYVNHYYSEPEANTGPSLIESDQEL 742
>TC14353 homologue to UP|Q8LL68 (Q8LL68) Latex plastidic aldolase-like
protein, partial (71%)
Length = 932
Score = 27.7 bits (60), Expect = 4.3
Identities = 11/34 (32%), Positives = 20/34 (58%)
Frame = +2
Query: 74 THDPKHHSSPSPFLPFLMKRTKVVEIVAAKNIVF 107
T P H +SPS +P LM ++ +++ + +VF
Sbjct: 179 TPPPTHQASPSELVPMLMSLLRLRKLLLHQGVVF 280
>TC11148 homologue to UP|Q7X9B8 (Q7X9B8) Ferrous ion membrane transport
protein DMT1, partial (33%)
Length = 622
Score = 27.3 bits (59), Expect = 5.6
Identities = 18/58 (31%), Positives = 31/58 (53%)
Frame = -2
Query: 26 SKPNIKINNKNIIKKLQTREISPKPHRSFVAATSPHRFQNMRLTHQFDTHDPKHHSSP 83
+KP IK +N N+ K I+ P+R+ ++ + N+R +H+P+HH SP
Sbjct: 576 NKP-IKSSNDNLSPKGHN-PITENPYRN----STTNNLLNIRTNESNLSHNPQHHPSP 421
>TC15179 similar to PIR|A31676|A31676 brain natriuretic factor precursor -
pig {Sus scrofa domestica;}, partial (10%)
Length = 674
Score = 27.3 bits (59), Expect = 5.6
Identities = 9/13 (69%), Positives = 11/13 (84%)
Frame = -3
Query: 350 LVTSFEDHLLWHP 362
L TSFE H++WHP
Sbjct: 396 LTTSFEAHMVWHP 358
>BP063972
Length = 524
Score = 26.9 bits (58), Expect = 7.3
Identities = 14/42 (33%), Positives = 25/42 (59%), Gaps = 2/42 (4%)
Frame = +2
Query: 257 LKIISIEDGT--VLKAFNHLLHRNKKVDFIEQFNEKLLVKQE 296
LKI ++ T + + F L +NK+ D ++QFN+ L+ K +
Sbjct: 272 LKIFRLQIMTEHIKEKFRKLKKQNKRNDMVQQFNQYLIQKPD 397
>TC11336 similar to UP|Q8S9A5 (Q8S9A5) Glucosyltransferase like protein
(Fragment), partial (27%)
Length = 511
Score = 26.9 bits (58), Expect = 7.3
Identities = 10/18 (55%), Positives = 12/18 (66%)
Frame = +2
Query: 77 PKHHSSPSPFLPFLMKRT 94
PKHHS+P P PF K +
Sbjct: 191 PKHHSNPHPPFPFTPKHS 244
>TC15231
Length = 753
Score = 26.6 bits (57), Expect = 9.5
Identities = 26/94 (27%), Positives = 45/94 (47%), Gaps = 9/94 (9%)
Frame = -1
Query: 111 HSGLCAAFS------RETNE-RICFLNI-CPDEVIRSLFYNKNNDSLITVS-VYASENFS 161
H+ +C F R+TN+ + FL I C D+ LF+ N T++ +Y + S
Sbjct: 657 HALICRGFKQGG*MGRKTNQTKESFLLIACLDQ----LFFQLNMK*NYTITHIYLALLNS 490
Query: 162 SLKCRSTRIEYIKRAKPDAGFPLFQSESLKWPGF 195
S R+ ++KR K G+P + ++W G+
Sbjct: 489 STPPYCIRL-FLKRTKMGVGWPRYHIHEIRWHGY 391
>TC10830 weakly similar to UP|Q94JL9 (Q94JL9) At1g68400/T2E12_5, partial
(24%)
Length = 702
Score = 26.6 bits (57), Expect = 9.5
Identities = 19/82 (23%), Positives = 33/82 (40%), Gaps = 14/82 (17%)
Frame = +1
Query: 48 PKPHRSFVAATSPH---------RFQNMRLTHQFDTHDP-----KHHSSPSPFLPFLMKR 93
P PH + + + P F++++ TH + H P + H P P LP ++
Sbjct: 166 PPPHSTSLHSHQPRFPPTNLLQSHFRHLQQTHHMELHHPPLHLARRHLPPKPRLPPRPRK 345
Query: 94 TKVVEIVAAKNIVFALAHSGLC 115
++ + N L HS C
Sbjct: 346 SRPPRLPPPLN----LPHSAPC 399
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.320 0.134 0.396
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,377,478
Number of Sequences: 28460
Number of extensions: 117092
Number of successful extensions: 754
Number of sequences better than 10.0: 38
Number of HSP's better than 10.0 without gapping: 750
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 752
length of query: 475
length of database: 4,897,600
effective HSP length: 94
effective length of query: 381
effective length of database: 2,222,360
effective search space: 846719160
effective search space used: 846719160
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 57 (26.6 bits)
Medicago: description of AC124215.9