BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC124215.6 - phase: 0 /pseudo
(679 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV767121 142 2e-34
AV780221 110 1e-24
TC18710 79 2e-15
AV780705 78 4e-15
TC18742 similar to UP|Q9FZG5 (Q9FZG5) T2E6.4, partial (3%) 58 6e-09
TC13572 46 3e-08
BP060613 44 1e-04
TC12829 40 0.001
CB828232 37 0.010
BP085058 33 0.12
TC12836 GB|CAA71302.1|2292921|LJPANC pantoate--beta-alanine liga... 32 0.34
TC13577 similar to UP|Q7VK23 (Q7VK23) Alpha-1,2-fucosyltransfera... 30 0.98
AV426437 30 1.7
BP085968 29 2.2
BP041113 29 2.8
TC8264 similar to GB|AAK20861.1|13377784|AF333974 fasciclin-like... 29 2.8
CB828813 28 3.7
AV769223 28 4.9
BP044109 28 6.3
AV781055 25 6.7
>AV767121
Length = 525
Score = 142 bits (357), Expect = 2e-34
Identities = 77/187 (41%), Positives = 111/187 (59%), Gaps = 3/187 (1%)
Frame = +2
Query: 239 LSSWKSKHLSLGGRLVLLKSVLSSLPVYALSFFKAPSGIVSSIESILNNFFFWGGWGGGS 298
LS WK K +S GGR+ L++SVL++LP++ LSFFK P G+ S ++ NF + GGS
Sbjct: 2 LSKWKQKTVSFGGRIFLIQSVLTALPLFFLSFFKLPIGVGKSCVRLMRNFLW-----GGS 166
Query: 299 ADHRKIIWVDWNSVCRSQEVGGLEVRRIKEFNLALLGKWCWRVLTERDSLWFRVLAVRNR 358
+ KI V W VC+ +E+GGL ++ + FN ALLGK WR LTE DSLW RV+
Sbjct: 167 ENENKIA*VKWTDVCKPKELGGLGIKDLFTFNKALLGK*RWRYLTEPDSLWRRVI----E 334
Query: 359 VEGGHLCSGGRNESMWWRNIVAL---HSEGWFQNHVSRSLGDGSSVLFWTDV*VGELSLR 415
+ H G S WW +I++L ++GWF + + + +G+G FW++ +G L
Sbjct: 335 AQPDHYSCG----SSWWNDILSLCPEDADGWFSSGLKKLVGEGDQTKFWSEDWLGSGLLS 502
Query: 416 DRFSRLY 422
RF RLY
Sbjct: 503 ARFRRLY 523
>AV780221
Length = 461
Score = 110 bits (274), Expect = 1e-24
Identities = 60/159 (37%), Positives = 95/159 (59%), Gaps = 5/159 (3%)
Frame = +3
Query: 253 LVLLKSVLSSLPVYALSFFKAPSG-IVSSIESILNNFFFWGGWGGGSADHRKIIWVDWNS 311
L L++SVL +LP++ L FFK P+G +++ + + FF GG +KI WV W++
Sbjct: 3 LCLIRSVLIALPLFHLPFFKLPTGGFYTNVNN**DPFFGREVEGG-----KKIAWVKWST 167
Query: 312 VCRSQEVGGLEVRRIKEFNLALLGKWCWRVLTERDSLWFRVLAVRNRVEGGHLCSGGRNE 371
VCR ++ GGL V+ + FN ALLGKW WR+L ERD LW++VL ++ + + ++
Sbjct: 168 VCRPKDEGGLGVQNLGLFNKALLGKWRWRMLKERDGLWYKVLLIK------YKNAIPQSA 329
Query: 372 SMWWRNIVALHSE----GWFQNHVSRSLGDGSSVLFWTD 406
S WW ++ + E GW Q + R +G+G+ V FW +
Sbjct: 330 SSWWNDLHSTCFEDGGGGWMQRGLCRRIGEGTEVKFWNE 446
>TC18710
Length = 843
Score = 79.3 bits (194), Expect = 2e-15
Identities = 47/130 (36%), Positives = 70/130 (53%), Gaps = 3/130 (2%)
Frame = +2
Query: 2 VQSAFIKGRQILDGILVANEV---VDDARKRKKDLLLFKVDFEKAYDSVD*NYLEEVMVK 58
+QS FI+GRQI D +L+ E ++ ++ + K+D KAYD V+ +LE ++
Sbjct: 455 MQSGFIQGRQIQDNLLIVQEAFHAINRPGALGRNHSIIKLDMNKAYDRVEWKFLESSLLA 634
Query: 59 MGFPTLWRKWIKKCVGTATASVLVNGSPTYEFSLHRGLRQGDPLSPFLFLLAAEGFHVLM 118
GF T W K I V + + +NG + RGLRQGDP SP+LFL E +
Sbjct: 635 FGFSTNWVKMIMILVSGVSYNYKINGVVGPKLLPQRGLRQGDPFSPYLFLFTME-----V 799
Query: 119 EALVVNNLFN 128
+L++ N FN
Sbjct: 800 LSLLIQNSFN 829
>AV780705
Length = 524
Score = 78.2 bits (191), Expect = 4e-15
Identities = 45/146 (30%), Positives = 79/146 (53%), Gaps = 2/146 (1%)
Frame = -2
Query: 210 PFLYLGLPI--GGNASRMVFWKPLINRINSRLSSWKSKHLSLGGRLVLLKSVLSSLPVYA 267
P YLGLP G N S + W + ++ +L WK L+ G+ VL+K+++ ++P YA
Sbjct: 493 PGHYLGLPAIWGRNKSHSLVW--IEEKVKEKLEGWKETLLNQAGKEVLIKAIIQAIPSYA 320
Query: 268 LSFFKAPSGIVSSIESILNNFFFWGGWGGGSADHRKIIWVDWNSVCRSQEVGGLEVRRIK 327
++ P +S+ +++ +F+ W G G+ ++ + S+E GG+ R ++
Sbjct: 319 MTIVHFPKTFCNSLYALVADFW-WKSQGEGAVEYTGAVGTK*PL---SKEKGGVGFRDLR 152
Query: 328 EFNLALLGKWCWRVLTERDSLWFRVL 353
NLA L + WRVLT ++LW RV+
Sbjct: 151 TQNLAFLARQAWRVLTNPEALWVRVM 74
>TC18742 similar to UP|Q9FZG5 (Q9FZG5) T2E6.4, partial (3%)
Length = 912
Score = 57.8 bits (138), Expect = 6e-09
Identities = 39/124 (31%), Positives = 64/124 (51%)
Frame = -3
Query: 140 VTHLQFADDTIILCDKSWANIRALRAILLLFQELSGLKVNFSKSLFVGVNVHGSWLAEAA 199
++HL FAD+ ++ + +I + +L +FQ LSGL N +KS V + V + +
Sbjct: 373 ISHLCFADNLMVFSNGYLESIAIINNVLHIFQHLSGLTPNPAKS-EVFILVKETTKNQIT 197
Query: 200 TVLNCNAGSIPFLYLGLPIGGNASRMVFWKPLINRINSRLSSWKSKHLSLGGRLVLLKSV 259
+L G +P LG+P + K L++R S+L W + LS GRL L+ S+
Sbjct: 196 NMLGYKEGKLPVR*LGVPQLTTKLKASDCKVLVDRKPSKLQHWTGRSLSYAGRLQLINSI 17
Query: 260 LSSL 263
L S+
Sbjct: 16 LFSM 5
>TC13572
Length = 534
Score = 46.2 bits (108), Expect(2) = 3e-08
Identities = 24/64 (37%), Positives = 34/64 (52%), Gaps = 2/64 (3%)
Frame = -3
Query: 367 GGRNESMWWRNIVALHS--EGWFQNHVSRSLGDGSSVLFWTDV*VGELSLRDRFSRLYDL 424
G N S WWR++ GWF+ V + L G+ FW + G LR+++ RLY+L
Sbjct: 442 GCTNISSWWRDVQTAGGGENGWFEEGVHKVLRSGNHTRFWLENWTGVGILREKYYRLYNL 263
Query: 425 SVLK 428
S LK
Sbjct: 262 SKLK 251
Score = 28.9 bits (63), Expect(2) = 3e-08
Identities = 17/32 (53%), Positives = 19/32 (59%)
Frame = -1
Query: 330 NLALLGKWCWRVLTERDSLWFRVLAVRNRVEG 361
N ALLGKW WR+ T +SL VL N V G
Sbjct: 534 NKALLGKWLWRLKTVPNSLGV-VLCWLNMVMG 442
>BP060613
Length = 378
Score = 43.5 bits (101), Expect = 1e-04
Identities = 28/101 (27%), Positives = 44/101 (42%), Gaps = 1/101 (0%)
Frame = +1
Query: 302 RKIIWVDWNSVCRSQEVGGLEVRRIKEFNLALLGKWCWRVLTERDSLWFRVLAVRNRVEG 361
R + W W+ + ++ GGL + + N ALL K WR+L D+LW ++L
Sbjct: 67 RGVHWRKWDLLTELKDEGGLGFKDFEIQNQALLAKQAWRILHNPDALWVQILKALYFPHH 246
Query: 362 GHLCSGGRNESMW-WRNIVALHSEGWFQNHVSRSLGDGSSV 401
L + R + W W ++ LH LG G +V
Sbjct: 247 DFLQTTKRTGASWVWSSL--LHGRELLLKQGQWQLGSGETV 363
>TC12829
Length = 448
Score = 40.0 bits (92), Expect = 0.001
Identities = 19/45 (42%), Positives = 26/45 (57%)
Frame = +3
Query: 245 KHLSLGGRLVLLKSVLSSLPVYALSFFKAPSGIVSSIESILNNFF 289
K L GR VL+KSV ++P Y +S F P I S IE ++ F+
Sbjct: 255 KFLYRAGREVLIKSVTKAIPTYIMSCFALPDSICSQIEGMVGKFY 389
>CB828232
Length = 532
Score = 37.0 bits (84), Expect = 0.010
Identities = 22/51 (43%), Positives = 30/51 (58%)
Frame = +1
Query: 242 WKSKHLSLGGRLVLLKSVLSSLPVYALSFFKAPSGIVSSIESILNNFFFWG 292
WK+ LS G R+ K VLSSLP++ LSFFK V+ + + + F WG
Sbjct: 388 WKTLCLS-GARVCCFKMVLSSLPLFFLSFFKIK--CVAKVCKQIQSRFLWG 531
>BP085058
Length = 452
Score = 33.5 bits (75), Expect = 0.12
Identities = 25/90 (27%), Positives = 43/90 (47%)
Frame = +2
Query: 232 INRINSRLSSWKSKHLSLGGRLVLLKSVLSSLPVYALSFFKAPSGIVSSIESILNNFFFW 291
++R+ SRL+ K+ L+ RL L K VLS++ +Y + P I I +++ + F W
Sbjct: 194 LDRVISRLAC*KAYLLNKPARLTLAKPVLSAISMYVMQLNWLPQSICDQICTVVRH-FVW 370
Query: 292 GGWGGGSADHRKIIWVDWNSVCRSQEVGGL 321
G I V W ++ + + GL
Sbjct: 371 ENVNG-------IPLVAWETIQSRKSMVGL 439
>TC12836 GB|CAA71302.1|2292921|LJPANC pantoate--beta-alanine ligase {Lotus
corniculatus var. japonicus;} , complete
Length = 1310
Score = 32.0 bits (71), Expect = 0.34
Identities = 16/41 (39%), Positives = 21/41 (51%)
Frame = +1
Query: 143 LQFADDTIILCDKSWANIRALRAILLLFQELSGLKVNFSKS 183
L F D II C + +R ILL++Q GL +N KS
Sbjct: 1102 LLFVGDGIIFCRATTPEENKIREILLMYQSAPGLLINLDKS 1224
>TC13577 similar to UP|Q7VK23 (Q7VK23) Alpha-1,2-fucosyltransferase, partial
(8%)
Length = 573
Score = 30.4 bits (67), Expect = 0.98
Identities = 9/24 (37%), Positives = 18/24 (74%)
Frame = +2
Query: 553 SILCWWDVDLLNHLIIYFYIVIFL 576
++ CW D ++L+H +IYF+ ++ L
Sbjct: 236 TVHCWRDANVLSHKVIYFFFLLLL 307
>AV426437
Length = 303
Score = 29.6 bits (65), Expect = 1.7
Identities = 9/25 (36%), Positives = 18/25 (72%)
Frame = +1
Query: 300 DHRKIIWVDWNSVCRSQEVGGLEVR 324
+ RKI W++W ++C+ + + GL V+
Sbjct: 217 NQRKIAWINWETICKPKWLRGLGVK 291
>BP085968
Length = 341
Score = 29.3 bits (64), Expect = 2.2
Identities = 19/56 (33%), Positives = 28/56 (49%)
Frame = +2
Query: 277 IVSSIESILNNFFFWGGWGGGSADHRKIIWVDWNSVCRSQEVGGLEVRRIKEFNLA 332
I+SS+ S F+F G+GG + +W W+S RSQ GL V + +A
Sbjct: 41 IMSSVWSDDGGFYFLYGFGGSG---KTFVWNTWSSGLRSQ---GLMVLNVASSGIA 190
>BP041113
Length = 503
Score = 28.9 bits (63), Expect = 2.8
Identities = 12/23 (52%), Positives = 18/23 (78%)
Frame = +1
Query: 403 FWTDV*VGELSLRDRFSRLYDLS 425
FWT+ +G +LRDR+ RL++LS
Sbjct: 1 FWTEDWIGMGTLRDRYYRLFNLS 69
>TC8264 similar to GB|AAK20861.1|13377784|AF333974 fasciclin-like
arabinogalactan-protein 9 {Arabidopsis thaliana;},
partial (38%)
Length = 942
Score = 28.9 bits (63), Expect = 2.8
Identities = 33/109 (30%), Positives = 45/109 (41%), Gaps = 3/109 (2%)
Frame = -2
Query: 391 VSRSLGDGSSVLFWTDV*VGELSLRDRFSRLYD---LSVLKGGVGRYDESVRLGGGG*GL 447
V+ L G V+ W D+ EL + D L VL+G VGR +E LGGG
Sbjct: 386 VAHCLEIGEGVVLWRDMVENELHFLV-IGEVIDGARLEVLEGVVGRGEEGEALGGG---- 222
Query: 448 EVEEKVVGLGGRVGRGA*AFTSKCFFSGSQKGWLEVECGFYVLLYSSKC 496
E + LG +G F + G G+ E +CG S+C
Sbjct: 221 --VELALNLGELLG-----FLEEADEGGVLPGFFE-DCG*VHRAGGSRC 99
>CB828813
Length = 465
Score = 28.5 bits (62), Expect = 3.7
Identities = 17/27 (62%), Positives = 18/27 (65%), Gaps = 3/27 (11%)
Frame = -2
Query: 440 LGGGG*G---LEVEEKVVGLGGRVGRG 463
LGGGG G LEVEE VG+GG G G
Sbjct: 314 LGGGGVGIGGLEVEEVRVGVGGGGGGG 234
>AV769223
Length = 389
Score = 28.1 bits (61), Expect = 4.9
Identities = 11/37 (29%), Positives = 21/37 (56%)
Frame = -3
Query: 470 KCFFSGSQKGWLEVECGFYVLLYSSKCNQNSHRAGSY 506
KC+ SG+ +G++ Y L+Y CN +H + ++
Sbjct: 144 KCYISGNWQGFMRTT---YCLIYCILCNSCTHESANF 43
>BP044109
Length = 497
Score = 27.7 bits (60), Expect = 6.3
Identities = 18/40 (45%), Positives = 22/40 (55%)
Frame = -3
Query: 424 LSVLKGGVGRYDESVRLGGGG*GLEVEEKVVGLGGRVGRG 463
+ V +GG GR + LGG G + VEE G GRVG G
Sbjct: 426 VGVARGGEGRVEPFGGLGGDGVEVGVEED--GGEGRVGAG 313
>AV781055
Length = 612
Score = 25.0 bits (53), Expect(2) = 6.7
Identities = 9/18 (50%), Positives = 12/18 (66%)
Frame = +2
Query: 62 PTLWRKWIKKCVGTATAS 79
P +WR+WIK + TAS
Sbjct: 314 PGMWREWIKDEISLKTAS 367
Score = 20.8 bits (42), Expect(2) = 6.7
Identities = 7/15 (46%), Positives = 11/15 (72%)
Frame = +1
Query: 76 ATASVLVNGSPTYEF 90
++A V NG+PTY +
Sbjct: 457 SSARVRANGTPTYSY 501
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.340 0.151 0.516
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,696,105
Number of Sequences: 28460
Number of extensions: 223427
Number of successful extensions: 1983
Number of sequences better than 10.0: 46
Number of HSP's better than 10.0 without gapping: 1937
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1974
length of query: 679
length of database: 4,897,600
effective HSP length: 96
effective length of query: 583
effective length of database: 2,165,440
effective search space: 1262451520
effective search space used: 1262451520
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.9 bits)
S2: 58 (26.9 bits)
Medicago: description of AC124215.6