BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC124214.17 - phase: 0
(362 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC16266 similar to UP|Q8VZI2 (Q8VZI2) AT4g33700/T16L1_190, parti... 543 e-155
CB829509 202 9e-53
TC12631 130 3e-31
TC20236 56 1e-08
TC11872 similar to UP|GTX6_SOYBN (P32110) Probable glutathione S... 31 0.37
BP042379 30 0.83
BU494388 30 0.83
TC14695 similar to UP|Q9FQE5 (Q9FQE5) Glutathione S-transferase ... 28 2.4
TC12777 similar to UP|Q9SYP6 (Q9SYP6) F9H16.10 protein, partial ... 27 5.4
TC16139 similar to UP|Q9SZ53 (Q9SZ53) Protein phosphatase 2C-lik... 27 5.4
TC10932 homologue to UP|Q39889 (Q39889) Heat shock protein , par... 26 9.1
TC16752 weakly similar to UP|AAQ96230 (AAQ96230) LRRGT00017, par... 26 9.1
BP085958 26 9.1
>TC16266 similar to UP|Q8VZI2 (Q8VZI2) AT4g33700/T16L1_190, partial (63%)
Length = 1193
Score = 543 bits (1399), Expect = e-155
Identities = 267/304 (87%), Positives = 287/304 (93%)
Frame = +2
Query: 59 RYGLAIGASVTPFVRVLVWICYPVAFPISKLLDYLLGHRNEALFRRAELKTLVDLHGNEA 118
RYGLAIGA V PFVRVLVWICYP+AFPISKLLD+LLGHR+EALFRRAELKTLV++HGNEA
Sbjct: 2 RYGLAIGALVAPFVRVLVWICYPIAFPISKLLDFLLGHRHEALFRRAELKTLVNMHGNEA 181
Query: 119 GKGGELTHDETTIIAGALELSEKTASDAMTPISETFAIDINSKLDRELMTEILEKGHSRV 178
GKGGELTHDETTIIAGALELS+KTASDAM+PI ETFAIDINSKLDRELM EILE GHSRV
Sbjct: 182 GKGGELTHDETTIIAGALELSDKTASDAMSPICETFAIDINSKLDRELMNEILENGHSRV 361
Query: 179 PVYYEQSTNIIGLILIKNLLTIHPEDESPVKSVTIRRIPRVPESMPLYDILNEFQKGHSH 238
PV+YEQ TNIIGLIL+KNLLTIHPEDE PVKSVTIRRIPRVPESMPLYDILNEFQKGHSH
Sbjct: 362 PVFYEQPTNIIGLILVKNLLTIHPEDEVPVKSVTIRRIPRVPESMPLYDILNEFQKGHSH 541
Query: 239 MAVVVRQCDKTKQPSSKNDSNDSVKEVKVDIDGEKPLQEKVLKPKIPIQKWKSFPNTNKS 298
MAVVVR+CDK +Q SS+N +N S + V VDIDGEKP QEKVLKP +P+ KWKSFPNTNKS
Sbjct: 542 MAVVVRRCDKNQQQSSENYANGSERYVTVDIDGEKPSQEKVLKPTMPLHKWKSFPNTNKS 721
Query: 299 NRGGSRSRKWSKNMYSDILEIDGSPLPNIPEEEEAVGIITMEDVIEELLQEEIFDETDHH 358
NRGGSRS+KWSKNMYSDILEIDG+PLP++PEEEEAVGIITMEDVIEELLQEEIFDETDHH
Sbjct: 722 NRGGSRSKKWSKNMYSDILEIDGNPLPHMPEEEEAVGIITMEDVIEELLQEEIFDETDHH 901
Query: 359 FEDS 362
FEDS
Sbjct: 902 FEDS 913
>CB829509
Length = 531
Score = 202 bits (513), Expect = 9e-53
Identities = 99/146 (67%), Positives = 119/146 (80%)
Frame = +3
Query: 107 LKTLVDLHGNEAGKGGELTHDETTIIAGALELSEKTASDAMTPISETFAIDINSKLDREL 166
LK LV +HG EAGKGGELTHDETTII+GAL+L+EKTA +AMTPI TF++D++SKLD E
Sbjct: 3 LKALVSIHGQEAGKGGELTHDETTIISGALDLTEKTAGEAMTPIESTFSLDVDSKLDWEA 182
Query: 167 MTEILEKGHSRVPVYYEQSTNIIGLILIKNLLTIHPEDESPVKSVTIRRIPRVPESMPLY 226
+ +IL +GHSRVPVY NIIGL+L+KNLLTI E E+PV +V+IRRIPRVP MPLY
Sbjct: 183 IGKILARGHSRVPVYNGNPKNIIGLLLVKNLLTIRAETETPVSAVSIRRIPRVPADMPLY 362
Query: 227 DILNEFQKGHSHMAVVVRQCDKTKQP 252
DILNEFQKG SHMA VV+ ++K P
Sbjct: 363 DILNEFQKGSSHMAAVVKVARESKNP 440
>TC12631
Length = 870
Score = 130 bits (327), Expect = 3e-31
Identities = 84/187 (44%), Positives = 106/187 (55%), Gaps = 6/187 (3%)
Frame = +1
Query: 180 VYYEQSTNIIGLILIKNLLTIHPEDESPVKSVTIRRIPRVPESMPLYDILNEFQKGHSHM 239
VY NIIGL+LIK+LLT+ PE E+PV +V+IRRIPRVP MPLYDILNEFQKG SHM
Sbjct: 16 VYSGNPKNIIGLLLIKSLLTVRPETETPVSAVSIRRIPRVPADMPLYDILNEFQKGSSHM 195
Query: 240 AVVVRQCDK--TKQPSSKNDSNDSVKEVKVDIDGEKPL----QEKVLKPKIPIQKWKSFP 293
A VV+ K T + D + V D PL EK+ + I+K P
Sbjct: 196 AAVVKARGKGITIPQIIDEEKYDEKESVGADSQLTTPLLQKQHEKLESVVVDIEKPSRHP 375
Query: 294 NTNKSNRGGSRSRKWSKNMYSDILEIDGSPLPNIPEEEEAVGIITMEDVIEELLQEEIFD 353
++NK + + GS +I E+ E +GIIT+EDV EELLQEEI D
Sbjct: 376 SSNKLQHSDATT--------------TGSFKESI-EDGEVIGIITLEDVFEELLQEEIVD 510
Query: 354 ETDHHFE 360
ETD + +
Sbjct: 511 ETDEYVD 531
>TC20236
Length = 463
Score = 55.8 bits (133), Expect = 1e-08
Identities = 24/47 (51%), Positives = 34/47 (72%)
Frame = +2
Query: 16 CGCWFYQALPIFLDSLVTAWGAILISVTLILLFGEIIPQSVCSRYGL 62
C +ALP++LD + A+L+SVT +L FGEIIPQ++C+RYGL
Sbjct: 320 CNACAMEALPLYLDKIFHPVVAVLLSVTFVLCFGEIIPQAICTRYGL 460
>TC11872 similar to UP|GTX6_SOYBN (P32110) Probable glutathione
S-transferase (Heat shock protein 26A) (G2-4) ,
complete
Length = 968
Score = 30.8 bits (68), Expect = 0.37
Identities = 23/102 (22%), Positives = 46/102 (44%), Gaps = 3/102 (2%)
Frame = +2
Query: 263 KEVKVDIDGEKPLQEKVLKPKIPIQKWKSFPNTNKSNRGGSRSRKWSKNMYSDILEIDGS 322
K+V V + +KP+ E ++ + + WKS P + +R WSK + +++
Sbjct: 185 KKVPVFVHNDKPIAESLVILEYIDETWKSNPILPSDPYKRALARFWSKFIDDKVIDTTWK 364
Query: 323 PLPNIPEEEEAVGIITMEDVIEEL---LQEEIFDETDHHFED 361
+ + E E I + + ++ L L+E+ F + F D
Sbjct: 365 AVFILEEAEREKIIEELSEALQYLENELKEKFFGGEEFEFVD 490
>BP042379
Length = 560
Score = 29.6 bits (65), Expect = 0.83
Identities = 20/75 (26%), Positives = 35/75 (46%), Gaps = 6/75 (8%)
Frame = +3
Query: 7 FLYGFNIQNCGCW-----FYQALPIFLDSLVT-AWGAILISVTLILLFGEIIPQSVCSRY 60
FL+ F +++ W F + P+ ++S A SVT +L G S+C+
Sbjct: 168 FLFSFILRSSASWALITSFLELFPVSMESFAALATSFFSFSVTNQVLLGREKISSLCAIM 347
Query: 61 GLAIGASVTPFVRVL 75
A AS++ F+R +
Sbjct: 348 FSAFAASISAFLRAI 392
>BU494388
Length = 350
Score = 29.6 bits (65), Expect = 0.83
Identities = 22/87 (25%), Positives = 36/87 (41%)
Frame = -3
Query: 254 SKNDSNDSVKEVKVDIDGEKPLQEKVLKPKIPIQKWKSFPNTNKSNRGGSRSRKWSKNMY 313
S D ++E+K +G P+ + +IP ++ SFP++ S S R WS
Sbjct: 279 SLEDKQALIRELKTKDEGLTPIVSIEVNTRIPSGRFFSFPHSKMS----SEKRLWSFTFS 112
Query: 314 SDILEIDGSPLPNIPEEEEAVGIITME 340
I S EE+ + +T E
Sbjct: 111 FSIFSKIHSKTKRALSEEKGMRDVTSE 31
>TC14695 similar to UP|Q9FQE5 (Q9FQE5) Glutathione S-transferase GST 13 ,
partial (97%)
Length = 1338
Score = 28.1 bits (61), Expect = 2.4
Identities = 25/97 (25%), Positives = 45/97 (45%), Gaps = 2/97 (2%)
Frame = +3
Query: 259 NDSVKEVKVDIDGEKPLQEKVLKPKIPIQKWKSFPNTNKSNRGGSRSRKWSKNMYSDILE 318
N K+V V + PL E ++ + + WK+ P + + +R WSK + IL
Sbjct: 225 NPVYKKVPVLVHNGNPLAESLVILEYIDETWKNSPFLPQDPYERAVARFWSKTLDDKIL- 401
Query: 319 IDGSPLPNIPEEEEAVGIITMEDVIE--ELLQEEIFD 353
P+ N +E +E+ +E +LLQ+++ D
Sbjct: 402 ---PPIWNACWSDEKGREKAVEEALEALKLLQDQLKD 503
>TC12777 similar to UP|Q9SYP6 (Q9SYP6) F9H16.10 protein, partial (10%)
Length = 500
Score = 26.9 bits (58), Expect = 5.4
Identities = 11/30 (36%), Positives = 20/30 (66%)
Frame = +3
Query: 135 ALELSEKTASDAMTPISETFAIDINSKLDR 164
ALELSE+T D + ++++F +N L++
Sbjct: 102 ALELSEQTVPDDLKALADSFMAKVNQGLEQ 191
>TC16139 similar to UP|Q9SZ53 (Q9SZ53) Protein phosphatase 2C-like protein
(AT4G31860/F11C18_60), partial (15%)
Length = 802
Score = 26.9 bits (58), Expect = 5.4
Identities = 12/44 (27%), Positives = 22/44 (49%)
Frame = +1
Query: 228 ILNEFQKGHSHMAVVVRQCDKTKQPSSKNDSNDSVKEVKVDIDG 271
IL +F+ + Q + + QPS + S+D+ E + +DG
Sbjct: 142 ILIQFKSSWNPDTSAANQTESSAQPSEADSSSDNQSEFQTSVDG 273
>TC10932 homologue to UP|Q39889 (Q39889) Heat shock protein , partial (28%)
Length = 1001
Score = 26.2 bits (56), Expect = 9.1
Identities = 28/121 (23%), Positives = 46/121 (37%), Gaps = 2/121 (1%)
Frame = +3
Query: 123 ELTHDETTIIAGALELSEKTASDAMTP--ISETFAIDINSKLDRELMTEILEKGHSRVPV 180
+ TH +AGA EL+ + MTP ++ T D N + + E+ V
Sbjct: 213 KFTHKTNEALAGAHELAMSSGHAQMTPLHLASTLISDPNGIFFQAISNSSGEESARAV-- 386
Query: 181 YYEQSTNIIGLILIKNLLTIHPEDESPVKSVTIRRIPRVPESMPLYDILNEFQKGHSHMA 240
++ L K P DE P + I+ I R + + +G +H+A
Sbjct: 387 -----ERVLNQALKKLPSQSPPPDEIPASTTLIKAIRRAQAA--------QKSRGDTHLA 527
Query: 241 V 241
V
Sbjct: 528 V 530
>TC16752 weakly similar to UP|AAQ96230 (AAQ96230) LRRGT00017, partial (23%)
Length = 525
Score = 26.2 bits (56), Expect = 9.1
Identities = 21/74 (28%), Positives = 33/74 (44%), Gaps = 12/74 (16%)
Frame = +3
Query: 257 DSNDSVKEVKVDIDGEKPLQE--KVL-----KPKIPIQKWKS-----FPNTNKSNRGGSR 304
D N +V +D + ++ K+L P P+Q++ FPN N+ NRGG
Sbjct: 267 DENTAVPRTHIDDEYANAAEKDPKILLTTSRDPSAPLQQFVKELKFVFPNANRMNRGG-- 440
Query: 305 SRKWSKNMYSDILE 318
+ SDI+E
Sbjct: 441 ------QVISDIIE 464
>BP085958
Length = 507
Score = 26.2 bits (56), Expect = 9.1
Identities = 10/26 (38%), Positives = 14/26 (53%)
Frame = +2
Query: 284 IPIQKWKSFPNTNKSNRGGSRSRKWS 309
+ +Q WK P+TN +N RS S
Sbjct: 65 LQLQPWKPSPSTNNNNNNNRRSENGS 142
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.318 0.138 0.403
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,179,222
Number of Sequences: 28460
Number of extensions: 82054
Number of successful extensions: 488
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 487
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 487
length of query: 362
length of database: 4,897,600
effective HSP length: 91
effective length of query: 271
effective length of database: 2,307,740
effective search space: 625397540
effective search space used: 625397540
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 56 (26.2 bits)
Medicago: description of AC124214.17