BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC123898.9 + phase: 0 /pseudo
(194 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC8891 similar to GB|AAP68279.1|31711846|BT008840 At1g72680 {Ara... 317 7e-88
TC10721 similar to UP|MTD_MEDSA (O82515) Probable mannitol dehyd... 121 6e-46
TC12763 similar to UP|CADH_MEDSA (P31656) Cinnamyl-alcohol dehyd... 144 1e-35
AV764937 143 2e-35
TC17332 similar to UP|MTD_FRAAN (Q9ZRF1) Probable mannitol dehyd... 112 4e-26
TC18136 similar to UP|CADH_MEDSA (P31656) Cinnamyl-alcohol dehyd... 42 7e-05
TC7966 similar to UP|Q84V25 (Q84V25) Quinone oxidoreductase, par... 38 0.001
TC7962 homologue to UP|Q84V97 (Q84V97) Alcohol dehydrogenase 1 ... 33 0.032
AV415744 33 0.032
BI418105 28 1.4
AV422565 27 1.8
TC17207 27 2.3
AU251635 27 3.0
BP038032 26 3.9
AV407318 25 6.7
TC9543 25 8.8
>TC8891 similar to GB|AAP68279.1|31711846|BT008840 At1g72680 {Arabidopsis
thaliana;} , complete
Length = 1636
Score = 317 bits (813), Expect = 7e-88
Identities = 158/190 (83%), Positives = 171/190 (89%)
Frame = +1
Query: 1 GSVYTFNCVDYDGTITKGGYSTSIVVNERYCFLIPKSYPLASAGPLLCAGITVYSPMMRH 60
GSV+TFN VD+DGTITKGGYS IVV+ERYCFLIPKSYPLASA PLLCAGITVYSPMMRH
Sbjct: 442 GSVFTFNGVDHDGTITKGGYSKFIVVHERYCFLIPKSYPLASAAPLLCAGITVYSPMMRH 621
Query: 61 NMNQPGKSLGVVGLGGLGHMAVKFGKAFGLRVTVFSTSMSKKEEALSFLGADQFVVSSNQ 120
MNQPGKSLGV+GLGGLGHMAVKFGKAFGL VTVFSTS+SKKEEALS LGAD+FVVSS+Q
Sbjct: 622 KMNQPGKSLGVIGLGGLGHMAVKFGKAFGLNVTVFSTSISKKEEALSLLGADRFVVSSDQ 801
Query: 121 EEMRALAKTLDFIIDTASGDHPFDPYMSLLKISGVLALVGFPSEVKFSPASLNLGSRTVA 180
EEMRALAK+LDFI+DTASG H FDPYM+LLK GVLALVGFP E+K P L +GSRTV+
Sbjct: 802 EEMRALAKSLDFIVDTASGPHSFDPYMALLKSFGVLALVGFPGEIKIHPGLLIMGSRTVS 981
Query: 181 GSAAGGTKEI 190
GS GGTKEI
Sbjct: 982 GSGVGGTKEI 1011
>TC10721 similar to UP|MTD_MEDSA (O82515) Probable mannitol dehydrogenase
(NAD-dependent mannitol dehydrogenase) , complete
Length = 1297
Score = 121 bits (303), Expect(2) = 6e-46
Identities = 65/125 (52%), Positives = 83/125 (66%), Gaps = 1/125 (0%)
Frame = +1
Query: 66 GKSLGVVGLGGLGHMAVKFGKAFGLRVTVFSTSMSKKEEALSFLGADQFVVSSNQEEMRA 125
GK LGV GLGGLGH+A+KFGKAFGL+VTV STS +K+ EA+ LGAD F+VS++ +M+A
Sbjct: 622 GKHLGVAGLGGLGHVAIKFGKAFGLKVTVISTSPNKEAEAIEKLGADSFLVSTDPAKMKA 801
Query: 126 LAKTLDFIIDTASGDHPFDPYMSLLKISGVLALVGFPSEVKFSPA-SLNLGSRTVAGSAA 184
T+ +IIDT S H P + LLK++G L VG P + P L G + V GS
Sbjct: 802 ALGTIHYIIDTISASHSLIPLLGLLKLNGKLVTVGLPGKPLELPVFPLVAGRKLVGGSHF 981
Query: 185 GGTKE 189
GG KE
Sbjct: 982 GGIKE 996
Score = 78.6 bits (192), Expect(2) = 6e-46
Identities = 37/68 (54%), Positives = 47/68 (68%)
Frame = +3
Query: 3 VYTFNCVDYDGTITKGGYSTSIVVNERYCFLIPKSYPLASAGPLLCAGITVYSPMMRHNM 62
VYT+N Y+GT T GGYS +VV++RY P + PL + PLLCAGITVY PM + M
Sbjct: 435 VYTYNS-PYNGTRTNGGYSDFVVVHQRYVLQFPDNLPLDAGAPLLCAGITVYXPMKYYGM 611
Query: 63 NQPGKSLG 70
+PGK+LG
Sbjct: 612 TEPGKTLG 635
>TC12763 similar to UP|CADH_MEDSA (P31656) Cinnamyl-alcohol dehydrogenase
(CAD) , partial (62%)
Length = 809
Score = 144 bits (362), Expect = 1e-35
Identities = 70/158 (44%), Positives = 102/158 (64%)
Frame = +2
Query: 3 VYTFNCVDYDGTITKGGYSTSIVVNERYCFLIPKSYPLASAGPLLCAGITVYSPMMRHNM 62
++ +N V DG T+GG++ +I+V +++ IP+ PLLCA +TVYSP+ +
Sbjct: 215 IWNYNDVYVDGKPTQGGFAETIIVEQKFVVKIPEGMAPEQVAPLLCAAVTVYSPLSHFGL 394
Query: 63 NQPGKSLGVVGLGGLGHMAVKFGKAFGLRVTVFSTSMSKKEEALSFLGADQFVVSSNQEE 122
+ G G++GLGG+GHM V KA G VTV S+S KK+EA+ LGAD ++VSS+
Sbjct: 395 KESGLRGGILGLGGVGHMGVIIAKAMGHHVTVISSSDRKKKEAIEDLGADAYLVSSDTTS 574
Query: 123 MRALAKTLDFIIDTASGDHPFDPYMSLLKISGVLALVG 160
M+ A +LD+IIDT HP +PY+SLLK+ G L L+G
Sbjct: 575 MQEAADSLDYIIDTVPVGHPLEPYLSLLKLDGKLILMG 688
>AV764937
Length = 392
Score = 143 bits (361), Expect = 2e-35
Identities = 70/129 (54%), Positives = 96/129 (74%), Gaps = 1/129 (0%)
Frame = +1
Query: 24 IVVNERYCFLIPKSYPLASAGPLLCAGITVYSPMMRHNM-NQPGKSLGVVGLGGLGHMAV 82
+V + RY IP++ P+ +A PLLCAGITV+SP+ H++ + PGK +GVVGLGGLGH+AV
Sbjct: 4 MVADYRYVVHIPENLPMDAAAPLLCAGITVFSPLKDHDLVSSPGKKIGVVGLGGLGHIAV 183
Query: 83 KFGKAFGLRVTVFSTSMSKKEEALSFLGADQFVVSSNQEEMRALAKTLDFIIDTASGDHP 142
KFGKAFG VTV STS SK+ EA LGAD F++S+NQ+ +++ ++LDF+ DT S +H
Sbjct: 184 KFGKAFGHHVTVISTSPSKEAEAKQRLGADDFILSTNQQHLQSARRSLDFVSDTVSAEHS 363
Query: 143 FDPYMSLLK 151
P + LLK
Sbjct: 364 LLPLLELLK 390
>TC17332 similar to UP|MTD_FRAAN (Q9ZRF1) Probable mannitol dehydrogenase
(NAD-dependent mannitol dehydrogenase) , partial (63%)
Length = 736
Score = 112 bits (280), Expect = 4e-26
Identities = 57/112 (50%), Positives = 75/112 (66%)
Frame = +1
Query: 3 VYTFNCVDYDGTITKGGYSTSIVVNERYCFLIPKSYPLASAGPLLCAGITVYSPMMRHNM 62
+ T+ + DGTIT GGYS +V +E + I + PL +A P LCAGITVYSP+ + +
Sbjct: 400 ILTYAAKNVDGTITYGGYSDLMVSDEDFGIRISDNLPLEAAAPFLCAGITVYSPLKYYGL 579
Query: 63 NQPGKSLGVVGLGGLGHMAVKFGKAFGLRVTVFSTSMSKKEEALSFLGADQF 114
++PG +GV GLGGLGHMAVKF A G +VT STS +KK EA+ + AD F
Sbjct: 580 DKPGLHVGVSGLGGLGHMAVKFAIALGAKVTAISTSPNKKTEAIEKV*ADSF 735
>TC18136 similar to UP|CADH_MEDSA (P31656) Cinnamyl-alcohol dehydrogenase
(CAD) , partial (28%)
Length = 601
Score = 42.0 bits (97), Expect = 7e-05
Identities = 21/50 (42%), Positives = 31/50 (62%), Gaps = 1/50 (2%)
Frame = +3
Query: 141 HPFDPYMSLLKISGVLALVG-FPSEVKFSPASLNLGSRTVAGSAAGGTKE 189
HP +PY+SLLK+ G L L+G + ++F + LG R++ GS G KE
Sbjct: 9 HPLEPYLSLLKLDGKLILMGVIYTPLQFITPMVMLGRRSITGSFIGSIKE 158
>TC7966 similar to UP|Q84V25 (Q84V25) Quinone oxidoreductase, partial (98%)
Length = 1908
Score = 38.1 bits (87), Expect = 0.001
Identities = 39/141 (27%), Positives = 59/141 (41%), Gaps = 2/141 (1%)
Frame = +2
Query: 7 NCVDYDGTITKGGYSTSIVVNERYCFLIPKSYPLASAGPLLCAGITVYSPMMRHNMNQPG 66
N ++Y + G + V E+ PK+ A L A T Y + R + G
Sbjct: 1016 NALEYPKVV--GSLAEYTAVEEKLLAHKPKNLSFVEAASLPLAIETAYEGLERAGFSA-G 1186
Query: 67 KSLGVVG-LGGLGHMAVKFGKAFGLRVTVFSTSMSKKEEALSFLGADQFVVSSNQEEMRA 125
KS+ V+G GG+G ++ K V +TS + K E L LGAD + +E +
Sbjct: 1187 KSILVLGGAGGVGTHVIQLAKHVFGASKVAATSSTGKLELLRKLGAD-MPIDYTKENVEN 1363
Query: 126 LAKTLDFIID-TASGDHPFDP 145
L + D + D D F P
Sbjct: 1364 LPEKFDVVFDAVGDADTTFKP 1426
>TC7962 homologue to UP|Q84V97 (Q84V97) Alcohol dehydrogenase 1 , complete
Length = 1489
Score = 33.1 bits (74), Expect = 0.032
Identities = 37/159 (23%), Positives = 64/159 (39%), Gaps = 9/159 (5%)
Frame = +1
Query: 39 PLASAGPLLCAGITVYSPMMRHNMNQPGKSLGVVGLGGLGHMAVKFGKAFGLRVTVFSTS 98
PL L C T + + +PG S+ + GLG +G A + + G +
Sbjct: 616 PLDKVCILSCGICTGFGATVNVAKPKPGSSVAIFGLGAVGLAAAEGARVSGASRIIGVDL 795
Query: 99 MSKKEEALSFLGADQFVVSSNQEE--MRALAKTLDFIID-----TASGDHPFDPYMSLLK 151
+S + E G ++FV + ++ +A+ + +D T S + +
Sbjct: 796 VSSRFEGAKKFGVNEFVNPKDHDKPVQEVIAEMTNGGVDRAVECTGSIQAMISAFECVHD 975
Query: 152 ISGVLALVGFPS-EVKFSPASLN-LGSRTVAGSAAGGTK 188
GV LVG P+ + F +N L RT+ G+ G K
Sbjct: 976 GWGVAVLVGVPNQDDAFQTHPVNFLNERTLKGTFYGNYK 1092
>AV415744
Length = 415
Score = 33.1 bits (74), Expect = 0.032
Identities = 34/119 (28%), Positives = 56/119 (46%), Gaps = 10/119 (8%)
Frame = +1
Query: 66 GKSLGVVGL-GGLGHMAVKFGKAFGLRVTVFSTSMSKKEEALSFLGADQFVVSSNQEEMR 124
G+ L V+G GG+G AV+ GKA G V + ++K + L G D V +N+
Sbjct: 52 GQVLLVLGAAGGVGLAAVQIGKACGAIVIAVARG-AEKVQLLKSHGVDHVVDLTNENVTE 228
Query: 125 AL--------AKTLDFIIDTASGDHPFDPYMSLLKISGVLALVGFPS-EVKFSPASLNL 174
++ K +D + D G + + LLK + ++GF S E+ PA++ L
Sbjct: 229 SVKEFLKARKLKGIDVLYDPVGGKLTKE-CLRLLKWGANILIIGFASGEIPVIPANIAL 402
>BI418105
Length = 600
Score = 27.7 bits (60), Expect = 1.4
Identities = 25/90 (27%), Positives = 42/90 (45%), Gaps = 16/90 (17%)
Frame = +3
Query: 56 PMMRHNMNQPG--KSLGVVGL---------GGLGHMAVKFGKAFGLRVTVFSTSMSKKEE 104
PM+ H M+QP K G +G+ + + KF K++G+RV+ MSK E+
Sbjct: 327 PMIVHIMDQPELQKEEGPIGVICAPTRELAQQIYLESKKFAKSYGIRVSAVYGGMSKLEQ 506
Query: 105 ALSFLGADQFVVSS-----NQEEMRALAKT 129
+ VV++ + +M+AL T
Sbjct: 507 FKELKAGCEIVVATPGRLIDMLKMKALTMT 596
>AV422565
Length = 470
Score = 27.3 bits (59), Expect = 1.8
Identities = 13/34 (38%), Positives = 20/34 (58%)
Frame = +3
Query: 4 YTFNCVDYDGTITKGGYSTSIVVNERYCFLIPKS 37
+T N +D G I +GGY + +N CFL+ K+
Sbjct: 213 FTLNIIDTPGLI-EGGYINDMALNLIKCFLLNKT 311
>TC17207
Length = 751
Score = 26.9 bits (58), Expect = 2.3
Identities = 11/22 (50%), Positives = 13/22 (59%)
Frame = +3
Query: 11 YDGTITKGGYSTSIVVNERYCF 32
+DG K GYS + VN YCF
Sbjct: 306 FDGK*QKVGYSPDMTVNNLYCF 371
>AU251635
Length = 383
Score = 26.6 bits (57), Expect = 3.0
Identities = 18/60 (30%), Positives = 28/60 (46%), Gaps = 1/60 (1%)
Frame = +3
Query: 64 QPGKSLGVVGLGGLGHMAVKFGKAFGL-RVTVFSTSMSKKEEALSFLGADQFVVSSNQEE 122
+PG + V GLG +G + KA G R+ +K E A +F G +F+ E+
Sbjct: 9 EPGSIVAVFGLGTVGLAVAEGAKAAGASRIIGIDIDSNKFERAKNF-GVTEFINPKEHEK 185
>BP038032
Length = 437
Score = 26.2 bits (56), Expect = 3.9
Identities = 11/21 (52%), Positives = 15/21 (71%)
Frame = +1
Query: 167 FSPASLNLGSRTVAGSAAGGT 187
FSP SL++ +AG +AGGT
Sbjct: 115 FSPLSLHVALSVMAGGSAGGT 177
>AV407318
Length = 428
Score = 25.4 bits (54), Expect = 6.7
Identities = 17/65 (26%), Positives = 28/65 (42%), Gaps = 2/65 (3%)
Frame = -3
Query: 15 ITKGGYSTSIVVNERYCFLIPKSYPLASAGPLLCAGITVYSP--MMRHNMNQPGKSLGVV 72
+T GG S +CF + S PL A +LCA + S ++ + + ++
Sbjct: 369 LTGGGTSLD------FCFKLKASLPLEPAWGVLCASLAFKSTDILLMEGLEDKDPDITLI 208
Query: 73 GLGGL 77
GL L
Sbjct: 207 GLESL 193
>TC9543
Length = 1003
Score = 25.0 bits (53), Expect = 8.8
Identities = 10/30 (33%), Positives = 18/30 (59%)
Frame = -1
Query: 37 SYPLASAGPLLCAGITVYSPMMRHNMNQPG 66
SY L + GP+ C ++P+M+ ++N G
Sbjct: 682 SYILPAGGPIPCKRNQFHNPVMKASINSNG 593
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.318 0.136 0.392
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,940,835
Number of Sequences: 28460
Number of extensions: 37260
Number of successful extensions: 180
Number of sequences better than 10.0: 32
Number of HSP's better than 10.0 without gapping: 179
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 179
length of query: 194
length of database: 4,897,600
effective HSP length: 85
effective length of query: 109
effective length of database: 2,478,500
effective search space: 270156500
effective search space used: 270156500
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)
Medicago: description of AC123898.9