BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC123593.3 + phase: 0 /pseudo
(176 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
NP863984 CEN/TFL1-like GTP-associated binding protein [Lotus cor... 184 7e-48
AU089275 78 7e-16
BP056744 27 1.5
TC9544 UP|AAO92441 (AAO92441) Homoglutathione synthetase, complete 26 4.4
TC15514 similar to UP|O23719 (O23719) MAP3K delta-1 protein kina... 26 4.4
TC9016 weakly similar to GB|AAM10351.1|20147275|AY093727 AT3g627... 25 5.8
TC11378 25 5.8
AV770138 25 7.5
TC9139 similar to UP|Q9LNA8 (Q9LNA8) F5O11.10, partial (12%) 25 7.5
BP044040 25 7.5
>NP863984 CEN/TFL1-like GTP-associated binding protein [Lotus corniculatus
var. japonicus]
Length = 525
Score = 184 bits (467), Expect = 7e-48
Identities = 90/167 (53%), Positives = 116/167 (68%)
Frame = +1
Query: 8 NPLAVGRVIGDVLDPFESTIPLLVTYGNRTVTNGGELKPSQVANQPQVIIGVNDPTALYT 67
+PL VGRV+GDVLD F +++ L V++ N+ V NG E PS + +P+V IG D + +T
Sbjct: 22 DPLVVGRVVGDVLDSFTTSMKLTVSFNNKQVFNGHEFFPSTINTKPKVDIGGGDMRSFFT 201
Query: 68 LVLVDPDAPSPSYPSFREYLHWMVTDIPATNAASFGNEVVSYEKPRPNLGIHRFVFVLLH 127
L++ DPD P PS P +E+LHWMVTDIP T A+FG E+VSYE P+PN+GIHRFVFVL
Sbjct: 202 LIMTDPDVPGPSDPYLKEHLHWMVTDIPGTTDATFGKELVSYEIPKPNIGIHRFVFVLFK 381
Query: 128 QQCRQRVYAPGWRQNFNTREFIEFYNLGSPVAAVFFNCQRETGSGGR 174
Q+ RQ V P R +FNTR F +L PVAAV+FN QRET + R
Sbjct: 382 QKRRQCVSPPSSRDHFNTRSFAAQNDLALPVAAVYFNAQRETAARRR 522
>AU089275
Length = 590
Score = 78.2 bits (191), Expect = 7e-16
Identities = 37/65 (56%), Positives = 44/65 (66%)
Frame = +3
Query: 103 GNEVVSYEKPRPNLGIHRFVFVLLHQQCRQRVYAPGWRQNFNTREFIEFYNLGSPVAAVF 162
G E+VSYE P+P +GIHR+VF+L Q+ RQ V P FNTR F E LG PVAAV+
Sbjct: 396 GKEIVSYESPKPVIGIHRYVFILFKQRGRQTVRPPASXDQFNTRSFSEXNGLGLPVAAVY 575
Query: 163 FNCQR 167
FN QR
Sbjct: 576 FNAQR 590
>BP056744
Length = 462
Score = 27.3 bits (59), Expect = 1.5
Identities = 21/77 (27%), Positives = 31/77 (39%), Gaps = 3/77 (3%)
Frame = +2
Query: 28 PLLVTYGN---RTVTNGGELKPSQVANQPQVIIGVNDPTALYTLVLVDPDAPSPSYPSFR 84
P L T GN R +T P + NQ Q + V A+Y L A + P+
Sbjct: 74 PSLTTRGNKI*RALTVFRRADPKNIGNQQQHQLKVEGKLAIYNSSLTSASASLTASPAGP 253
Query: 85 EYLHWMVTDIPATNAAS 101
E + +PA+ + S
Sbjct: 254 EVDASSLVSLPASTSVS 304
>TC9544 UP|AAO92441 (AAO92441) Homoglutathione synthetase, complete
Length = 1860
Score = 25.8 bits (55), Expect = 4.4
Identities = 10/26 (38%), Positives = 17/26 (64%)
Frame = -2
Query: 100 ASFGNEVVSYEKPRPNLGIHRFVFVL 125
A FGN V +++P N+G+ +F+ L
Sbjct: 1229 AQFGNIVFVFKEPLKNIGLRKFLLNL 1152
>TC15514 similar to UP|O23719 (O23719) MAP3K delta-1 protein kinase
(Fragment), partial (18%)
Length = 706
Score = 25.8 bits (55), Expect = 4.4
Identities = 10/30 (33%), Positives = 16/30 (53%)
Frame = -2
Query: 104 NEVVSYEKPRPNLGIHRFVFVLLHQQCRQR 133
N ++ + +PR N I ++ LH CR R
Sbjct: 327 NRLLWFFEPRDNFSIITIIYAQLHLMCRAR 238
>TC9016 weakly similar to GB|AAM10351.1|20147275|AY093727
AT3g62730/F26K9_160 {Arabidopsis thaliana;}, partial
(37%)
Length = 571
Score = 25.4 bits (54), Expect = 5.8
Identities = 15/28 (53%), Positives = 18/28 (63%)
Frame = +3
Query: 106 VVSYEKPRPNLGIHRFVFVLLHQQCRQR 133
+V Y R NLG HR VFVLLH + R +
Sbjct: 468 IVYYNNIR-NLG-HRIVFVLLHYRFRSK 545
>TC11378
Length = 683
Score = 25.4 bits (54), Expect = 5.8
Identities = 20/94 (21%), Positives = 43/94 (45%), Gaps = 3/94 (3%)
Frame = +1
Query: 36 RTVTNGGELKPSQVANQPQVIIGVN---DPTALYTLVLVDPDAPSPSYPSFREYLHWMVT 92
+ ++GG L PSQ+ + V++ VN T + ++L+D + + +H+ +
Sbjct: 202 KRTSSGGFLFPSQLLSLTLVVVAVN*SASSTEVLAIILLDKIL-------YHQMVHYSRS 360
Query: 93 DIPATNAASFGNEVVSYEKPRPNLGIHRFVFVLL 126
+ NE++SY+ N F+ +L+
Sbjct: 361 QL---------NEIMSYQLSIKNYMDKTFIILLV 435
>AV770138
Length = 483
Score = 25.0 bits (53), Expect = 7.5
Identities = 10/23 (43%), Positives = 15/23 (64%)
Frame = -1
Query: 118 IHRFVFVLLHQQCRQRVYAPGWR 140
IH+F ++LHQ CR + + WR
Sbjct: 120 IHQFQLIILHQFCR-KSFCAYWR 55
>TC9139 similar to UP|Q9LNA8 (Q9LNA8) F5O11.10, partial (12%)
Length = 1084
Score = 25.0 bits (53), Expect = 7.5
Identities = 13/38 (34%), Positives = 18/38 (47%)
Frame = +1
Query: 126 LHQQCRQRVYAPGWRQNFNTREFIEFYNLGSPVAAVFF 163
+H QC + +NF FIEF+ S + VFF
Sbjct: 337 IHPQCNNKFCVI*SVRNFQFLGFIEFFPQPSSFSCVFF 450
>BP044040
Length = 491
Score = 25.0 bits (53), Expect = 7.5
Identities = 14/31 (45%), Positives = 17/31 (54%)
Frame = +3
Query: 7 PNPLAVGRVIGDVLDPFESTIPLLVTYGNRT 37
P+P R GD DPF+S LVT NR+
Sbjct: 285 PSPQKTNRNPGDGFDPFDS----LVTSSNRS 365
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.320 0.139 0.429
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,185,873
Number of Sequences: 28460
Number of extensions: 45901
Number of successful extensions: 192
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 190
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 192
length of query: 176
length of database: 4,897,600
effective HSP length: 84
effective length of query: 92
effective length of database: 2,506,960
effective search space: 230640320
effective search space used: 230640320
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)
Medicago: description of AC123593.3