BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC123574.8 - phase: 0 /pseudo
(621 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV777094 29 2.0
TC19965 homologue to UP|Q8VZF3 (Q8VZF3) At2g47390/T8I13.23, part... 28 3.3
AV407628 28 4.4
AV774725 28 4.4
TC19871 28 5.7
TC13766 weakly similar to AAQ83299 (AAQ83299) QRT3, partial (9%) 27 7.5
TC8909 similar to UP|Q9FIJ4 (Q9FIJ4) Gb|AAF26028.1, partial (69%) 27 7.5
AV420955 27 7.5
TC19050 weakly similar to UP|Q9M6T9 (Q9M6T9) Nonspecific lipid-t... 27 9.7
TC9895 similar to UP|Q41695 (Q41695) Pectinacetylesterase precur... 27 9.7
TC9726 weakly similar to UP|Q93Y18 (Q93Y18) SNF1 related protein... 27 9.7
AV779849 27 9.7
>AV777094
Length = 223
Score = 29.3 bits (64), Expect = 2.0
Identities = 14/42 (33%), Positives = 20/42 (47%), Gaps = 5/42 (11%)
Frame = -2
Query: 175 YFGLEFSNCGFATVWFCFCLHGLDSN-----YLTICPSFDSC 211
Y GL +C ++ +C HG+DSN + CP D C
Sbjct: 135 YLGLALPSCHMFSI---YCEHGIDSNIGPLLIFSFCPRCDQC 19
>TC19965 homologue to UP|Q8VZF3 (Q8VZF3) At2g47390/T8I13.23, partial (17%)
Length = 503
Score = 28.5 bits (62), Expect = 3.3
Identities = 12/29 (41%), Positives = 16/29 (54%)
Frame = +3
Query: 239 CRGGS*QGDFFGSQCRKNCAYVILLWCFH 267
C GGS +GS ++NC + LWC H
Sbjct: 390 CSGGSHSA--WGSSSKQNCCWWPFLWCVH 470
>AV407628
Length = 426
Score = 28.1 bits (61), Expect = 4.4
Identities = 25/82 (30%), Positives = 35/82 (42%), Gaps = 11/82 (13%)
Frame = +1
Query: 224 GCLAGGSSFPTSILHCRGGS*QGDFFGSQCRKNCAYVILLWCFH-SHSHIVYG*CDDFLC 282
G AGG + S* G +C + +++ FH SH H+ G C LC
Sbjct: 34 GSRAGGDDGRRLFTNYAD*S*TGSVSVLRCVVTASCDLVVHSFHWSHWHLQRGWCHIGLC 213
Query: 283 RY*----------AKHSLFASY 294
R * ++HSLFA+Y
Sbjct: 214 RA*FCLC*QFCFRSRHSLFANY 279
>AV774725
Length = 555
Score = 28.1 bits (61), Expect = 4.4
Identities = 13/28 (46%), Positives = 17/28 (60%)
Frame = -1
Query: 257 CAYVILLWCFHSHSHIVYG*CDDFLCRY 284
C ++L+ FH HSH + G DFLC Y
Sbjct: 510 CCLLVLI--FHVHSHFLVG---DFLCNY 442
>TC19871
Length = 528
Score = 27.7 bits (60), Expect = 5.7
Identities = 10/30 (33%), Positives = 17/30 (56%)
Frame = +3
Query: 560 FLKSCLMCPKWQHALVPWCCPIVIFRICWF 589
+L S C ++ H+L+ WC ++I WF
Sbjct: 135 YLSSAFTCLEFNHSLISWCFNLII----WF 212
>TC13766 weakly similar to AAQ83299 (AAQ83299) QRT3, partial (9%)
Length = 543
Score = 27.3 bits (59), Expect = 7.5
Identities = 11/24 (45%), Positives = 15/24 (61%)
Frame = +2
Query: 251 SQCRKNCAYVILLWCFHSHSHIVY 274
S CR NCAY++ C SH I++
Sbjct: 311 SDCRGNCAYLMKELCILSHIFILH 382
>TC8909 similar to UP|Q9FIJ4 (Q9FIJ4) Gb|AAF26028.1, partial (69%)
Length = 1000
Score = 27.3 bits (59), Expect = 7.5
Identities = 10/27 (37%), Positives = 12/27 (44%)
Frame = +2
Query: 553 FCTENGTFLKSCLMCPKWQHALVPWCC 579
FCT + +S C W H WCC
Sbjct: 839 FCTNSNALCESTFFCITWVHF---WCC 910
>AV420955
Length = 394
Score = 27.3 bits (59), Expect = 7.5
Identities = 13/24 (54%), Positives = 14/24 (58%)
Frame = +2
Query: 499 KGTYWFGCCQFTLGCWNGRKYSFF 522
KGT GCC F C GRK+S F
Sbjct: 38 KGTRTKGCCCFIFTC-EGRKFSIF 106
>TC19050 weakly similar to UP|Q9M6T9 (Q9M6T9) Nonspecific lipid-transfer
protein (LTP), partial (57%)
Length = 579
Score = 26.9 bits (58), Expect = 9.7
Identities = 15/43 (34%), Positives = 19/43 (43%), Gaps = 3/43 (6%)
Frame = +1
Query: 176 FGLEFSNCGFA---TVWFCFCLHGLDSNYLTICPSFDSCQWQR 215
FGL F CG A T +C +H L + CP QW +
Sbjct: 79 FGLSFRRCGSAMWPTYVYCCTMHWLPEEPYSNCPC-SMLQWDK 204
>TC9895 similar to UP|Q41695 (Q41695) Pectinacetylesterase precursor ,
partial (43%)
Length = 713
Score = 26.9 bits (58), Expect = 9.7
Identities = 13/45 (28%), Positives = 25/45 (54%)
Frame = -3
Query: 459 IISLEAHVGKFSVVSVASKEIFI*WQAYSALCKIVGVVWYKGTYW 503
I SL++ + + +K *+++Y+AL K +GV+ T+W
Sbjct: 228 ISSLQSRQVPWGSATPGAKMFLT*YESYAALTKKIGVLICPATFW 94
>TC9726 weakly similar to UP|Q93Y18 (Q93Y18) SNF1 related protein kinase,
partial (6%)
Length = 641
Score = 26.9 bits (58), Expect = 9.7
Identities = 16/65 (24%), Positives = 27/65 (40%)
Frame = +2
Query: 458 VIISLEAHVGKFSVVSVASKEIFI*WQAYSALCKIVGVVWYKGTYWFGCCQFTLGCWNGR 517
V + +GK + + ++ + + + IV V G +WFG LG W+ R
Sbjct: 305 VSLGFRVEIGKNGAIGLGKGKVGVVVEVFQIASPIVVGVSQIGGWWFGV*LD*LGKWSSR 484
Query: 518 KYSFF 522
S F
Sbjct: 485 CCSLF 499
>AV779849
Length = 465
Score = 26.9 bits (58), Expect = 9.7
Identities = 33/115 (28%), Positives = 47/115 (40%), Gaps = 32/115 (27%)
Frame = -3
Query: 192 FCLHGLDSNYLTICPSFDSCQWQR-----SGVLSLLQGCLAGGSSFPTSI------LHCR 240
FC+ DS L + ++ SC W S V+S L + GS + +HC
Sbjct: 367 FCVRERDSPPLLLYCAWASCLWTNTFIWFSCVMS*LSLSVRLGSGYFLHS*MILYPVHCL 188
Query: 241 -GGS*QG---------DFFGSQCRKNCAYVILL-----------WCFHSHSHIVY 274
GGS*QG +FF + +C Y ILL + FHS S+ +
Sbjct: 187 IGGS*QGRNYR*WETDNFFVCKFCTSCYYYILLVEF*VHLTL*VFTFHSSSYFTF 23
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.365 0.166 0.686
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 14,154,293
Number of Sequences: 28460
Number of extensions: 256463
Number of successful extensions: 4331
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 2398
Number of HSP's successfully gapped in prelim test: 161
Number of HSP's that attempted gapping in prelim test: 1832
Number of HSP's gapped (non-prelim): 2746
length of query: 621
length of database: 4,897,600
effective HSP length: 96
effective length of query: 525
effective length of database: 2,165,440
effective search space: 1136856000
effective search space used: 1136856000
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 14 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 36 (21.5 bits)
S2: 58 (26.9 bits)
Medicago: description of AC123574.8