BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC123574.2 + phase: 0 /pseudo
(984 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC13186 weakly similar to UP|Q8W2C0 (Q8W2C0) Functional candidat... 145 4e-35
AV779547 120 8e-28
AV406755 120 1e-27
CB828263 114 1e-25
BP045440 108 5e-24
TC19146 similar to UP|Q8S4X3 (Q8S4X3) TIR-similar-domain-contain... 102 4e-22
BP065014 91 1e-18
AI967395 77 1e-14
AV778571 75 5e-14
BP065724 74 9e-14
TC12663 weakly similar to UP|Q84ZV8 (Q84ZV8) R 3 protein, partia... 59 6e-12
TC9932 weakly similar to UP|Q93YA7 (Q93YA7) Resistance gene-like... 66 2e-11
TC15876 weakly similar to UP|Q8S4X3 (Q8S4X3) TIR-similar-domain-... 58 6e-09
TC20188 weakly similar to UP|Q8S4X3 (Q8S4X3) TIR-similar-domain-... 57 2e-08
AV406565 53 2e-07
TC12592 50 1e-06
AW428699 49 5e-06
AV408271 46 3e-05
BP068235 36 3e-04
AV423544 40 0.001
>TC13186 weakly similar to UP|Q8W2C0 (Q8W2C0) Functional candidate
resistance protein KR1, partial (6%)
Length = 586
Score = 145 bits (365), Expect = 4e-35
Identities = 78/150 (52%), Positives = 107/150 (71%), Gaps = 1/150 (0%)
Frame = +1
Query: 14 FKYQVFLSFRGSDTRYGFTGNLYKALTDKGIHTFIDDSELQRGDEITPSLDN-AIEESRI 72
F Y VF+S SDTR+GF+G+LY AL+DKGI TFIDD L R D+I ++++ AIE SRI
Sbjct: 97 FIYDVFISLITSDTRFGFSGDLYTALSDKGILTFIDDG-LPRPDDIMSTVNSKAIESSRI 273
Query: 73 FIPVFSANYASSSFCLDELVHIIHLYKQNGRLVLPVFFGVDPSHVRHHRGSYGEALAKHE 132
I V S NYASSSFCLDEL++I+ ++ G L+ PVF+ V+PS VR+ +G +G+A A HE
Sbjct: 274 AIVVISENYASSSFCLDELMYILKRREEKGLLIWPVFYQVEPSDVRYQKGGFGKAFASHE 453
Query: 133 ERFQHNTDHMERLQKWKIALTQAANLSGDH 162
ERF ++ +++KW+ AL Q A+L H
Sbjct: 454 ERFGSDS---AKVRKWRNALKQVADLKAFH 534
>AV779547
Length = 556
Score = 120 bits (302), Expect = 8e-28
Identities = 69/141 (48%), Positives = 88/141 (61%)
Frame = +1
Query: 20 LSFRGSDTRYGFTGNLYKALTDKGIHTFIDDSELQRGDEITPSLDNAIEESRIFIPVFSA 79
+SFRG DTR FT +L AL KG TF DD+ L++G I+ L AIE S+I I VFS
Sbjct: 109 VSFRGEDTRNNFTDHLLGALYGKGFVTFKDDTMLRKGKNISTELIQAIEGSQILIVVFSK 288
Query: 80 NYASSSFCLDELVHIIHLYKQNGRLVLPVFFGVDPSHVRHHRGSYGEALAKHEERFQHNT 139
YASS++CL EL I + VLPV V PS VR G+YGEA KHEERF+
Sbjct: 289 YYASSTWCLQELAKIADGIVGKRQTVLPVVCDVTPSEVRKQSGNYGEAFLKHEERFK--- 459
Query: 140 DHMERLQKWKIALTQAANLSG 160
+ +E +Q+W+ AL + A+LSG
Sbjct: 460 EDLEMVQRWRKALAEVADLSG 522
>AV406755
Length = 425
Score = 120 bits (300), Expect = 1e-27
Identities = 62/117 (52%), Positives = 81/117 (68%)
Frame = +3
Query: 14 FKYQVFLSFRGSDTRYGFTGNLYKALTDKGIHTFIDDSELQRGDEITPSLDNAIEESRIF 73
+ Y VFLSFRG ++R FT +LY AL + GI F+D+ ELQRG++I+ SL AIE+SRI
Sbjct: 78 WSYDVFLSFRGEESRRSFTSHLYTALKNAGIKVFMDN-ELQRGEDISSSLLKAIEDSRIA 254
Query: 74 IPVFSANYASSSFCLDELVHIIHLYKQNGRLVLPVFFGVDPSHVRHHRGSYGEALAK 130
I +FS NY S +CLDEL II + G+ V+PVF+ VDPS +R RG+ GEA K
Sbjct: 255 IIIFSTNYTGSKWCLDELEKIIECQRTIGQEVMPVFYNVDPSDIRKQRGTVGEAFRK 425
>CB828263
Length = 570
Score = 114 bits (284), Expect = 1e-25
Identities = 64/151 (42%), Positives = 95/151 (62%), Gaps = 5/151 (3%)
Frame = +2
Query: 52 ELQRGDEITPSLDNAIEESRIFIPVFSANYASSSFCLDELVHIIHLYKQNGRLVLPVFFG 111
+LQRGDEI+ SL AIEE+++ + VFS NY +S +CLDELV I+ + G++VLPVF+
Sbjct: 5 DLQRGDEISSSLLRAIEEAKLSVIVFSKNYGNSKWCLDELVKILECKRTRGQIVLPVFYD 184
Query: 112 VDPSHVRHHRGSYGEALAKHEERFQHNTDHMERLQKWKIALTQAANLSG-DHRSPGYEYK 170
+DPSHVR+ G+Y EA KH + ++++QKW+ AL +AANLSG D E +
Sbjct: 185 IDPSHVRNQTGTYAEAFVKHGQ--------VDKVQKWREALREAANLSGWDCSVNRMESE 340
Query: 171 LTGKIAFNQTPDLS----SDCSQSLVEHEQI 197
+ KIA + L+ D + + + EQ+
Sbjct: 341 VIEKIAKDVLEKLNRVYVGDLDEKIAKFEQL 433
>BP045440
Length = 535
Score = 108 bits (269), Expect = 5e-24
Identities = 66/148 (44%), Positives = 84/148 (56%), Gaps = 2/148 (1%)
Frame = -3
Query: 15 KYQVFLSFRGSDTRYGFTGNLYKALTDKGIHTFIDDSELQRGDEITPSLDNAIEESRIFI 74
+YQ+F+SFRG DTR FT LY+ L +G TF DD L+ G I LD+ IEESR I
Sbjct: 530 RYQIFISFRGMDTRDDFTKPLYQGLCREGFVTFKDDESLEGGSPIEKLLDD-IEESRFAI 354
Query: 75 PVFSANYASSSFCLDELVHIIHLYKQNGR--LVLPVFFGVDPSHVRHHRGSYGEALAKHE 132
+ S NYA S +CL EL I+ Q G+ LVLP V P+ VRH Y +A+AKHE
Sbjct: 353 IILSKNYAESEWCLKELSKILECKDQEGKNQLVLPSSTEVTPTEVRHVINRYADAMAKHE 174
Query: 133 ERFQHNTDHMERLQKWKIALTQAANLSG 160
+ N + +Q WK AL L+G
Sbjct: 173 K----NGIDSKTIQAWKKALFDVCTLTG 102
>TC19146 similar to UP|Q8S4X3 (Q8S4X3) TIR-similar-domain-containing protein
TSDC, partial (12%)
Length = 701
Score = 102 bits (253), Expect = 4e-22
Identities = 59/142 (41%), Positives = 84/142 (58%), Gaps = 1/142 (0%)
Frame = +1
Query: 35 LYKALTDKGIHTFIDDSELQRGDEITPSLDNAIEESRIFIPVFSANYASSSFCLDELVHI 94
LY AL +G TF+DD L G+EI SL +A E+SR+ I VFS NY SS+CLDELV I
Sbjct: 1 LYHALRREGFKTFMDDEGLVGGNEIAKSLLDASEKSRLSIIVFSENYGYSSWCLDELVKI 180
Query: 95 IHLYKQNGRLVLPVFFGVDPSHVRHHRGSYGEALAKHEERFQHNTDHMERLQKWKIALTQ 154
+ + +LV P+F+ V+ S V SY A+ HE+ + ++D ++KW+ AL+
Sbjct: 181 VECMETKKQLVWPIFYKVEKSDVTSQENSYEHAMIGHEKNYGKDSD---EVKKWRSALSA 351
Query: 155 AANLSGDH-RSPGYEYKLTGKI 175
A L G + + YEY+ KI
Sbjct: 352 MAKLEGHNVKENKYEYEFIKKI 417
>BP065014
Length = 498
Score = 90.5 bits (223), Expect = 1e-18
Identities = 52/126 (41%), Positives = 79/126 (62%)
Frame = -2
Query: 47 FIDDSELQRGDEITPSLDNAIEESRIFIPVFSANYASSSFCLDELVHIIHLYKQNGRLVL 106
F DD L+ G I L AIEESR+ I V S N+A S +CL ELV I+ K+ LVL
Sbjct: 497 FKDDLSLEDGAPIEELL-GAIEESRVAIVVLSENFADSKWCLKELVKILDCRKKKKPLVL 321
Query: 107 PVFFGVDPSHVRHHRGSYGEALAKHEERFQHNTDHMERLQKWKIALTQAANLSGDHRSPG 166
P+F+ V+P+++RH +G YGEA+A+HE++ ++ + +++W AL+ + L G+ G
Sbjct: 320 PIFYKVEPTNIRHLKGRYGEAMAEHEKKLGKDS---QTVREWTQALSDISYLKGEPYK-G 153
Query: 167 YEYKLT 172
Y KL+
Sbjct: 152 YVVKLS 135
>AI967395
Length = 205
Score = 77.4 bits (189), Expect = 1e-14
Identities = 36/67 (53%), Positives = 50/67 (73%)
Frame = +3
Query: 67 IEESRIFIPVFSANYASSSFCLDELVHIIHLYKQNGRLVLPVFFGVDPSHVRHHRGSYGE 126
I+ESR+ I VFS +YA S+ CL+ELV+I+ + N +LV PV++GV+ +RH RG YGE
Sbjct: 3 IQESRLIIVVFSEHYAVSASCLNELVNIVDVMIMNNQLVWPVYYGVEAPEIRHQRGRYGE 182
Query: 127 ALAKHEE 133
A+AK EE
Sbjct: 183 AMAKFEE 203
>AV778571
Length = 452
Score = 75.1 bits (183), Expect = 5e-14
Identities = 51/119 (42%), Positives = 65/119 (53%)
Frame = +1
Query: 655 VNI*HIYLMSQVFQI*RSSHLNFVEI*LQLTIQLATKINLNS*VQLVAASSSVFHP*VWH 714
VN+*H YL+ V QI*++ HL V I* + T QL + INL S + V A S +FHP* W
Sbjct: 46 VNL*HKYLICLVSQI*KNYHLYIVRI*SKSTSQLDS*INLESWILRVVARSGLFHP*SWP 225
Query: 715 LLRNWNSLFV*VSIVFQNYYAR*QI*NVFCLLILP*ENCHLHFKISVNLMTYQ*RDVEC 773
LL+N VFQ Y R + *++ LP* NCHL +IS+ VEC
Sbjct: 226 LLKNCIFHIAQALRVFQKY*QRWKT*HLLVCTTLP*RNCHLRLEISLGFEG*NCMAVEC 402
>BP065724
Length = 495
Score = 74.3 bits (181), Expect = 9e-14
Identities = 36/91 (39%), Positives = 58/91 (63%)
Frame = -1
Query: 81 YASSSFCLDELVHIIHLYKQNGRLVLPVFFGVDPSHVRHHRGSYGEALAKHEERFQHNTD 140
YA SS+CL+E+V I+ ++N +LV P+F+ V+PS VRH R SY +A+ K E R+ ++
Sbjct: 495 YADSSWCLEEVVKILECKQKNDQLVWPIFYKVEPSDVRHQRNSYEKAMIKQETRYGKDS- 319
Query: 141 HMERLQKWKIALTQAANLSGDHRSPGYEYKL 171
E+++KW+ +L+Q +L H Y L
Sbjct: 318 --EKVKKWRSSLSQVCDLKAFHYKENSGYVL 232
>TC12663 weakly similar to UP|Q84ZV8 (Q84ZV8) R 3 protein, partial (4%)
Length = 494
Score = 58.9 bits (141), Expect(2) = 6e-12
Identities = 28/45 (62%), Positives = 35/45 (77%)
Frame = +3
Query: 11 SYVFKYQVFLSFRGSDTRYGFTGNLYKALTDKGIHTFIDDSELQR 55
S+V + VFL+FRGSDT +GF L K+L DKGI+TFI+D ELQR
Sbjct: 12 SHVLTFNVFLNFRGSDTCHGFISFLCKSLLDKGIYTFINDEELQR 146
Score = 29.3 bits (64), Expect(2) = 6e-12
Identities = 14/19 (73%), Positives = 15/19 (78%)
Frame = +2
Query: 57 DEITPSLDNAIEESRIFIP 75
DEITPSL AIE +RI IP
Sbjct: 149 DEITPSLVKAIERTRIAIP 205
>TC9932 weakly similar to UP|Q93YA7 (Q93YA7) Resistance gene-like, partial
(5%)
Length = 689
Score = 66.2 bits (160), Expect = 2e-11
Identities = 30/64 (46%), Positives = 44/64 (67%)
Frame = -2
Query: 4 SSSSSSISYVFKYQVFLSFRGSDTRYGFTGNLYKALTDKGIHTFIDDSELQRGDEITPSL 63
S SSSS ++ + Y VF++FRG DTR+ F +LY AL++ G++ F+DD L+RG E+ P L
Sbjct: 238 SMSSSSSNHQWIYDVFINFRGKDTRHSFVSHLYTALSNAGVNVFLDDENLERGLELEPEL 59
Query: 64 DNAI 67
I
Sbjct: 58 QKLI 47
>TC15876 weakly similar to UP|Q8S4X3 (Q8S4X3) TIR-similar-domain-containing
protein TSDC, partial (13%)
Length = 558
Score = 58.2 bits (139), Expect = 6e-09
Identities = 29/77 (37%), Positives = 49/77 (62%), Gaps = 2/77 (2%)
Frame = +1
Query: 94 IIHLYKQNGRLVLPVFFGVDPSHVRHHRGSYGEALAKHEERFQHNTDHMERLQKWKIALT 153
I+ ++N +LV P+F+ V+PS VRH R SY +A+ K E R+ ++ E+++KW+ +L+
Sbjct: 16 ILECXQKNDQLVWPIFYKVEPSDVRHQRNSYEKAMIKPETRYGKDS---EKVKKWRSSLS 186
Query: 154 QAANLSGDH--RSPGYE 168
Q +L H + GYE
Sbjct: 187 QVCDLKAFHYKENSGYE 237
>TC20188 weakly similar to UP|Q8S4X3 (Q8S4X3) TIR-similar-domain-containing
protein TSDC, partial (11%)
Length = 494
Score = 56.6 bits (135), Expect = 2e-08
Identities = 31/74 (41%), Positives = 45/74 (59%), Gaps = 1/74 (1%)
Frame = +1
Query: 103 RLVLPVFFGVDPSHVRHHRGSYGEALAKHEERFQHNTDHMERLQKWKIALTQAANLSGDH 162
+LV P+F+ + S V + SYG A+ HE F ++ E++QKWK AL++ A L G+H
Sbjct: 4 QLVWPIFYEISXSDVSNQTKSYGHAMLAHENSFGKDS---EKVQKWKSALSEMAFLQGEH 174
Query: 163 RSPG-YEYKLTGKI 175
+ YEYKL KI
Sbjct: 175 ITENEYEYKLIQKI 216
>AV406565
Length = 207
Score = 53.1 bits (126), Expect = 2e-07
Identities = 30/55 (54%), Positives = 38/55 (68%)
Frame = +2
Query: 5 SSSSSISYVFKYQVFLSFRGSDTRYGFTGNLYKALTDKGIHTFIDDSELQRGDEI 59
S++SS + K+ VFLSFRG DTRY TG+L LT + T+I D +LQRGDEI
Sbjct: 44 STTSSSAPQQKHDVFLSFRGEDTRYTCTGHLQATLTRLQVKTYI-DYDLQRGDEI 205
>TC12592
Length = 534
Score = 50.4 bits (119), Expect = 1e-06
Identities = 23/59 (38%), Positives = 41/59 (68%)
Frame = +3
Query: 103 RLVLPVFFGVDPSHVRHHRGSYGEALAKHEERFQHNTDHMERLQKWKIALTQAANLSGD 161
+LVLPVF+GV+P+ +R+ +G +GEA+A+ E +F +++ + +WK AL + L G+
Sbjct: 3 QLVLPVFYGVEPTDIRNLKGKFGEAMAEIENKFGKDSN---TIHEWKQALRDISYLKGE 170
>AW428699
Length = 319
Score = 48.5 bits (114), Expect = 5e-06
Identities = 22/42 (52%), Positives = 27/42 (63%)
Frame = +2
Query: 471 CLSGHCLLLQRMRMGGCQRYTPCSLWPLHNTSSRSVG*KISH 512
C S HCLL +R+ +G C YT CSLW L +S+G* ISH
Sbjct: 146 CFS*HCLLRRRVSLGRCSTYTSCSLWLLRYRLHKSIG**ISH 271
>AV408271
Length = 409
Score = 46.2 bits (108), Expect = 3e-05
Identities = 24/45 (53%), Positives = 30/45 (66%)
Frame = +1
Query: 15 KYQVFLSFRGSDTRYGFTGNLYKALTDKGIHTFIDDSELQRGDEI 59
KY VF+SFRG D R GF +L A K I+ F+DD +L+RG EI
Sbjct: 277 KYDVFVSFRGEDIRDGFLSHLADAFRQKKINAFMDD-KLKRGQEI 408
>BP068235
Length = 511
Score = 35.8 bits (81), Expect(2) = 3e-04
Identities = 22/66 (33%), Positives = 35/66 (52%), Gaps = 1/66 (1%)
Frame = -1
Query: 88 LDELVHIIHLYKQNGRLVLPVFFGVDPSHVRHHRGSYGEALA-KHEERFQHNTDHMERLQ 146
L L + K +L+ P + V+PS +R+ SYG ALA + F +++ E++Q
Sbjct: 475 LMNLSRFLECKKMKNQLLWPCPYKVEPSDIRYRPSSYGTALA*TRTDAFGNDS---EKIQ 305
Query: 147 KWKIAL 152
WK AL
Sbjct: 304 TWKSAL 287
Score = 26.2 bits (56), Expect(2) = 3e-04
Identities = 12/17 (70%), Positives = 13/17 (75%)
Frame = -3
Query: 78 SANYASSSFCLDELVHI 94
S NYA S+ CLDELV I
Sbjct: 506 SENYAYSARCLDELVQI 456
>AV423544
Length = 443
Score = 40.4 bits (93), Expect = 0.001
Identities = 25/72 (34%), Positives = 42/72 (57%)
Frame = +1
Query: 895 RIYGIKYESEMDKALLENEWIRVELELKSFNLSEEEKNEMLRSAQMGIHVLKEKNNAEEE 954
++ G K++ ++D+ALLENEW E+ L SF + GIH+ K+K++ E
Sbjct: 154 QLQGRKFK-DIDQALLENEWNHAEIILVSF---------LSTFIGTGIHIFKQKSSI--E 297
Query: 955 NVIFTDPYRETK 966
++ FT PY++ K
Sbjct: 298 HIRFTCPYKKRK 333
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.355 0.155 0.549
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 18,417,126
Number of Sequences: 28460
Number of extensions: 281367
Number of successful extensions: 3538
Number of sequences better than 10.0: 48
Number of HSP's better than 10.0 without gapping: 2581
Number of HSP's successfully gapped in prelim test: 92
Number of HSP's that attempted gapping in prelim test: 899
Number of HSP's gapped (non-prelim): 2757
length of query: 984
length of database: 4,897,600
effective HSP length: 99
effective length of query: 885
effective length of database: 2,080,060
effective search space: 1840853100
effective search space used: 1840853100
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 14 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 37 (21.6 bits)
S2: 60 (27.7 bits)
Medicago: description of AC123574.2