BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC122728.13 + phase: 0
(164 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
CB828504 231 5e-62
TC17290 similar to UP|Q8H4Y5 (Q8H4Y5) OJ1136_A10.4 protein, part... 28 1.0
TC16028 27 2.3
TC14770 similar to PIR|T52133|T52133 potassium channel beta subu... 25 6.7
AV770842 25 6.7
TC9667 similar to PIR|T52133|T52133 potassium channel beta subun... 25 6.7
BP067421 25 8.7
TC10519 similar to UP|Q94FT4 (Q94FT4) Salutaridinol 7-O-acetyltr... 25 8.7
CN825408 25 8.7
TC16084 similar to GB|AAL84954.1|19310435|AY078954 F17H15.1/F17H... 25 8.7
TC15574 homologue to UP|RL31_NICGU (P46290) 60S ribosomal protei... 25 8.7
>CB828504
Length = 495
Score = 231 bits (589), Expect = 5e-62
Identities = 111/134 (82%), Positives = 120/134 (88%)
Frame = +2
Query: 1 MKLVFESEKAEWIGTDKVIGCGFDPRQKKVFFTLDSELMHVVHCQTEEFSTPLYPTLAAN 60
MKLVFESEKAEWIG DKVIGCG DPRQKKVFFTLDSELMHV+HCQ+E F TPLYPTLAAN
Sbjct: 92 MKLVFESEKAEWIGADKVIGCGXDPRQKKVFFTLDSELMHVIHCQSEGFGTPLYPTLAAN 271
Query: 61 IDIMVLVNFGQNAFKYAPANAQRTPNPCLVSPLVSSPAAIGYDDDSKELFSMGRIDSHWL 120
IDI VLVNFGQ+AFKYAPANAQRTPNPC ++PLV+SP + Y DDSKELFSMGRIDS WL
Sbjct: 272 IDIQVLVNFGQSAFKYAPANAQRTPNPCFIAPLVNSP-TLSY-DDSKELFSMGRIDSQWL 445
Query: 121 NRTTTKGSHNNGNN 134
NR+ TKGSHN+ N
Sbjct: 446 NRSATKGSHNSNGN 487
>TC17290 similar to UP|Q8H4Y5 (Q8H4Y5) OJ1136_A10.4 protein, partial (14%)
Length = 540
Score = 27.7 bits (60), Expect = 1.0
Identities = 23/77 (29%), Positives = 34/77 (43%), Gaps = 16/77 (20%)
Frame = +2
Query: 94 VSSPAAIG-YDDDSKELFSMGRIDSHWLNRTTTKGSH-------NNGNNIS--------T 137
+ +P G YDD E FS+ ++ S W T++ G GN +S
Sbjct: 155 IKTPVKTGIYDDLYGESFSV-KVGSSWAYSTSSTGERASSDKEDEKGNTLSGKSDSKPSN 331
Query: 138 MDFDEESEADLFEIVID 154
D DEE + DLF ++D
Sbjct: 332 ADGDEEDDDDLFG*LLD 382
>TC16028
Length = 593
Score = 26.6 bits (57), Expect = 2.3
Identities = 13/42 (30%), Positives = 20/42 (46%)
Frame = -3
Query: 89 LVSPLVSSPAAIGYDDDSKELFSMGRIDSHWLNRTTTKGSHN 130
+++ + + P IG DD +E S G+I W N HN
Sbjct: 237 MIAYINTHPTRIGRDDRGREETSRGKIGEKWRNPRRRICIHN 112
>TC14770 similar to PIR|T52133|T52133 potassium channel beta subunit homolog
[imported] - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (75%)
Length = 1144
Score = 25.0 bits (53), Expect = 6.7
Identities = 10/26 (38%), Positives = 16/26 (61%)
Frame = +2
Query: 34 LDSELMHVVHCQTEEFSTPLYPTLAA 59
LD E + V++C + STP+ T+ A
Sbjct: 140 LDMEYVDVIYCHRPDTSTPIEETVRA 217
>AV770842
Length = 536
Score = 25.0 bits (53), Expect = 6.7
Identities = 10/21 (47%), Positives = 13/21 (61%)
Frame = +3
Query: 77 APANAQRTPNPCLVSPLVSSP 97
AP+N R+ NPC+ P SP
Sbjct: 18 APSNKTRSTNPCISLPST*SP 80
>TC9667 similar to PIR|T52133|T52133 potassium channel beta subunit homolog
[imported] - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (51%)
Length = 574
Score = 25.0 bits (53), Expect = 6.7
Identities = 10/26 (38%), Positives = 16/26 (61%)
Frame = +2
Query: 34 LDSELMHVVHCQTEEFSTPLYPTLAA 59
LD E + V++C + STP+ T+ A
Sbjct: 401 LDMEYVDVIYCHRPDTSTPIEETVRA 478
>BP067421
Length = 409
Score = 24.6 bits (52), Expect = 8.7
Identities = 14/30 (46%), Positives = 16/30 (52%)
Frame = +2
Query: 56 TLAANIDIMVLVNFGQNAFKYAPANAQRTP 85
TLAA I LV + Q A + APA A P
Sbjct: 242 TLAAKHSIFSLVKWAQIA*RIAPAKASPAP 331
>TC10519 similar to UP|Q94FT4 (Q94FT4) Salutaridinol 7-O-acetyltransferase
, partial (4%)
Length = 616
Score = 24.6 bits (52), Expect = 8.7
Identities = 9/16 (56%), Positives = 12/16 (74%)
Frame = +2
Query: 21 CGFDPRQKKVFFTLDS 36
CGFD ++K+ FFT S
Sbjct: 143 CGFDLKEKEFFFTYTS 190
>CN825408
Length = 675
Score = 24.6 bits (52), Expect = 8.7
Identities = 11/33 (33%), Positives = 21/33 (63%)
Frame = -1
Query: 37 ELMHVVHCQTEEFSTPLYPTLAANIDIMVLVNF 69
EL+ +HC++ + TPL L+A + ++ L N+
Sbjct: 183 ELLPQLHCRSSKPQTPLSDHLSAFLGLLQLRNW 85
>TC16084 similar to GB|AAL84954.1|19310435|AY078954 F17H15.1/F17H15.1
{Arabidopsis thaliana;}, partial (29%)
Length = 889
Score = 24.6 bits (52), Expect = 8.7
Identities = 10/27 (37%), Positives = 15/27 (55%)
Frame = +2
Query: 112 MGRIDSHWLNRTTTKGSHNNGNNISTM 138
+ R+ S W + T + H N +N STM
Sbjct: 8 LSRVCSRWQRKRTMQHRHRNPSNGSTM 88
>TC15574 homologue to UP|RL31_NICGU (P46290) 60S ribosomal protein L31,
partial (95%)
Length = 647
Score = 24.6 bits (52), Expect = 8.7
Identities = 12/32 (37%), Positives = 18/32 (55%)
Frame = -3
Query: 63 IMVLVNFGQNAFKYAPANAQRTPNPCLVSPLV 94
I+++V NA+ Y P N TP+P + LV
Sbjct: 339 ILIIVPLTSNAYPYPPGN---TPDPLAPNSLV 253
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.316 0.132 0.392
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,593,719
Number of Sequences: 28460
Number of extensions: 30401
Number of successful extensions: 185
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 184
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 184
length of query: 164
length of database: 4,897,600
effective HSP length: 83
effective length of query: 81
effective length of database: 2,535,420
effective search space: 205369020
effective search space used: 205369020
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 52 (24.6 bits)
Medicago: description of AC122728.13