BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC122727.10 - phase: 1 /pseudo
(136 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV766619 35 0.006
TC12653 similar to UP|BIOB_ARATH (P54967) Biotin synthase (Biot... 30 0.14
TC11794 similar to AAP86662 (AAP86662) 26S proteasome subunit RP... 29 0.25
TC16381 27 1.2
TC9042 weakly similar to PIR|G86367|G86367 protein F28C11.3 [imp... 26 2.1
TC13923 homologue to UP|Q38950 (Q38950) Protein phosphatase 2A 6... 26 2.7
TC8018 25 3.6
TC14892 similar to UP|Q9Z0W6 (Q9Z0W6) Pax transcription activati... 25 3.6
TC13609 25 4.6
TC11120 UP|Q84PP4 (Q84PP4) Transcription factor MYB102, complete 25 6.1
TC17495 membrane transporter [Lotus japonicus] 24 7.9
>AV766619
Length = 412
Score = 34.7 bits (78), Expect = 0.006
Identities = 15/57 (26%), Positives = 25/57 (43%)
Frame = +2
Query: 59 YSMTQPSITHYRNQIRDWSDDVVKWVDDNPKKQWLQAHDQGRRWGHITTNISECFNN 115
Y+ Q + ++ S + WV N + W A G R+ H+T+N + F N
Sbjct: 197 YAPKQETFERSVENMKGISSEAFNWVMQNEPEHWANAFFNGARYNHLTSNFGQQFYN 367
>TC12653 similar to UP|BIOB_ARATH (P54967) Biotin synthase (Biotin
synthetase) , partial (29%)
Length = 535
Score = 30.0 bits (66), Expect = 0.14
Identities = 21/77 (27%), Positives = 32/77 (41%), Gaps = 5/77 (6%)
Frame = +1
Query: 59 YSMTQPSITHYRNQIR-----DWSDDVVKWVDDNPKKQWLQAHDQGRRWGHITTNISECF 113
YS + P+ I+ DW+ D VK + D+P L Q R H + +C
Sbjct: 202 YSSSSPAAIQAERTIKQGPRNDWTRDEVKSIYDSPVLDLLFHGAQVHRHAHNFREVQQCT 381
Query: 114 NNVLKGGPQSSNHTNCP 130
+K G S + + CP
Sbjct: 382 LLSVKTGGCSEDCSYCP 432
>TC11794 similar to AAP86662 (AAP86662) 26S proteasome subunit RPN6a,
partial (19%)
Length = 435
Score = 29.3 bits (64), Expect = 0.25
Identities = 11/24 (45%), Positives = 15/24 (61%)
Frame = -2
Query: 80 VVKWVDDNPKKQWLQAHDQGRRWG 103
++ W +D K WLQ +QGRR G
Sbjct: 167 ILWWQEDKMMKTWLQTPEQGRRGG 96
>TC16381
Length = 655
Score = 26.9 bits (58), Expect = 1.2
Identities = 12/26 (46%), Positives = 18/26 (69%), Gaps = 3/26 (11%)
Frame = -1
Query: 77 SDDVVKWVDDNPKKQWLQA---HDQG 99
S+D +K + D PK +WLQ+ H+QG
Sbjct: 271 SEDKLKQLLDQPKNKWLQSLTLHEQG 194
>TC9042 weakly similar to PIR|G86367|G86367 protein F28C11.3 [imported] -
Arabidopsis thaliana {Arabidopsis thaliana;}, partial
(12%)
Length = 768
Score = 26.2 bits (56), Expect = 2.1
Identities = 10/22 (45%), Positives = 13/22 (58%)
Frame = +2
Query: 87 NPKKQWLQAHDQGRRWGHITTN 108
+P K WL H Q R H+TT+
Sbjct: 212 SPVKPWLTVHTQSPRAPHVTTS 277
>TC13923 homologue to UP|Q38950 (Q38950) Protein phosphatase 2A 65 kDa
regulatory subunit, partial (20%)
Length = 515
Score = 25.8 bits (55), Expect = 2.7
Identities = 12/46 (26%), Positives = 25/46 (54%), Gaps = 3/46 (6%)
Frame = -2
Query: 47 NTEMKKKVRNMG-YSMTQPSITHYRNQIRDWSD--DVVKWVDDNPK 89
N++++ +V + + + + ++ R W D+VKWV +NPK
Sbjct: 151 NSKVRFRVLRLSIFXFGETKLQRRESRYRKWERVVDLVKWVSENPK 14
>TC8018
Length = 592
Score = 25.4 bits (54), Expect = 3.6
Identities = 18/72 (25%), Positives = 31/72 (43%), Gaps = 2/72 (2%)
Frame = -2
Query: 48 TEMKKKVRNMGYSM--TQPSITHYRNQIRDWSDDVVKWVDDNPKKQWLQAHDQGRRWGHI 105
+ + KK M Y+M T H QI + + + K + N ++W Q + H
Sbjct: 540 SSIHKKRILMSYNMQCTHEQQKHEEAQINESTPKMKKEGEINYYRKWRQHRQFRS*FAHD 361
Query: 106 TTNISECFNNVL 117
T + +CF +L
Sbjct: 360 TRLLGQCFKKIL 325
>TC14892 similar to UP|Q9Z0W6 (Q9Z0W6) Pax transcription activation domain
interacting protein PTIP, partial (3%)
Length = 1502
Score = 25.4 bits (54), Expect = 3.6
Identities = 10/21 (47%), Positives = 15/21 (70%)
Frame = +2
Query: 52 KKVRNMGYSMTQPSITHYRNQ 72
+KVRN+GY Q ++ H R+Q
Sbjct: 863 QKVRNLGYLRFQHALQHQRSQ 925
>TC13609
Length = 356
Score = 25.0 bits (53), Expect = 4.6
Identities = 12/30 (40%), Positives = 16/30 (53%)
Frame = -2
Query: 28 IGTMSSAFDT*LNFLRTFKNTEMKKKVRNM 57
+ T S DT L R++KN KKK R +
Sbjct: 319 VPTTSDRMDTFLGISRSYKNRSRKKKHRKI 230
>TC11120 UP|Q84PP4 (Q84PP4) Transcription factor MYB102, complete
Length = 1281
Score = 24.6 bits (52), Expect = 6.1
Identities = 14/48 (29%), Positives = 26/48 (54%), Gaps = 5/48 (10%)
Frame = +2
Query: 17 QSKVLLGV*TVIGTMSSAFDT*L-----NFLRTFKNTEMKKKVRNMGY 59
+ + +L + +++G S T L N ++ F NT +KKK+ MG+
Sbjct: 287 KEQTILHLHSILGNKWSTIATHLPGRTDNEIKNFWNTHLKKKLIQMGF 430
>TC17495 membrane transporter [Lotus japonicus]
Length = 979
Score = 24.3 bits (51), Expect = 7.9
Identities = 15/41 (36%), Positives = 19/41 (45%), Gaps = 6/41 (14%)
Frame = -2
Query: 96 HDQGRRWGHITT------NISECFNNVLKGGPQSSNHTNCP 130
H Q W I T + S+C + GPQSS+HT P
Sbjct: 246 HGQHSNWKRIRTPKPYTEHSSKCLED--PQGPQSSSHTKQP 130
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.329 0.139 0.467
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,899,003
Number of Sequences: 28460
Number of extensions: 40244
Number of successful extensions: 227
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 225
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 227
length of query: 136
length of database: 4,897,600
effective HSP length: 81
effective length of query: 55
effective length of database: 2,592,340
effective search space: 142578700
effective search space used: 142578700
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 50 (23.9 bits)
Medicago: description of AC122727.10