BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC122725.14 + phase: 0
(512 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP043046 188 2e-48
TC17100 similar to UP|MID2_YEAST (P36027) Mating process protein... 92 3e-19
AV422763 88 4e-18
CN825203 77 7e-15
AU089369 75 3e-14
AV410889 70 6e-13
TC10842 57 7e-09
AV429083 49 1e-06
AV427280 46 2e-05
CB826792 45 2e-05
AV417736 40 7e-04
AV421795 40 0.001
TC15322 weakly similar to UP|Q9LG23 (Q9LG23) F14J16.14 (At1g5589... 38 0.005
AV767789 37 0.006
BP044414 36 0.017
BI418759 35 0.029
TC13348 weakly similar to UP|Q9LUJ4 (Q9LUJ4) Gb|AAF26800.1, part... 34 0.065
BU494388 33 0.085
AV779633 33 0.085
BF177625 33 0.11
>BP043046
Length = 516
Score = 188 bits (478), Expect = 2e-48
Identities = 95/112 (84%), Positives = 104/112 (92%)
Frame = -3
Query: 401 DVKKFLHYFGKAISSVKQWIPDPRLVQEAFTVIQEQAHIEGAEQLLVILRNAGHVNTNIY 460
DV KFL YF KAISSVK+W PD +LV+EAF +I+EQAHIEGAEQLLVILR+A +VNT+IY
Sbjct: 514 DVVKFLDYFKKAISSVKKWSPDQKLVEEAFKIIEEQAHIEGAEQLLVILRDASYVNTSIY 335
Query: 461 NLFLKTYAAAGKMPLVVAERMKKDNVQLDKETHRLLDLTSKMCVSDVSGILS 512
NL LKTYAAAGKMPLVVAERMKKDNVQLD+ETHRLLDLTSKMCVSDVSGILS
Sbjct: 334 NLLLKTYAAAGKMPLVVAERMKKDNVQLDEETHRLLDLTSKMCVSDVSGILS 179
>TC17100 similar to UP|MID2_YEAST (P36027) Mating process protein MID2
(Serine-rich protein SMS1) (Protein kinase A
interference protein), partial (7%)
Length = 704
Score = 91.7 bits (226), Expect = 3e-19
Identities = 51/164 (31%), Positives = 83/164 (50%), Gaps = 3/164 (1%)
Frame = +3
Query: 336 ENLYKEWESVSGTNDVRVSNLLLTSYVDQGQMEMAEIFCNQLVEKGVCLSYSSWELLTRG 395
E L EWE T D RV N+LL Y +G +E AE +VEKG + SW ++ G
Sbjct: 9 EKLLGEWELSGNTYDFRVPNILLIGYSKKGLIEKAETMLRSMVEKGKTPNPYSWSIVASG 188
Query: 396 YLKKKDVKKFLHYFGKAISSV---KQWIPDPRLVQEAFTVIQEQAHIEGAEQLLVILRNA 452
Y+ +++++K +A++ + K W P P +V + + + IE E + L+
Sbjct: 189 YVAQENMEKAFQCLKEALAVLAENKGWRPKPNVVSSILSWVTDNRDIEEVEDFVNSLKKV 368
Query: 453 GHVNTNIYNLFLKTYAAAGKMPLVVAERMKKDNVQLDKETHRLL 496
+N ++Y +K + GK V E MK DN++LD+E +L
Sbjct: 369 MSMNRDMYRSLIKLHVRCGKEVDGVLESMKADNIELDEELEEIL 500
>AV422763
Length = 410
Score = 87.8 bits (216), Expect = 4e-18
Identities = 55/143 (38%), Positives = 83/143 (57%), Gaps = 5/143 (3%)
Frame = +2
Query: 197 EELKVNTSPDVVTFNLLLTACASENDVETAERVLLQLKKAKVDP----DWVTYSTLTNLY 252
+E+K N + TF + L A + DVE E++L+Q++ DP DW TYST N Y
Sbjct: 5 QEMKENDLCNGATFTIRLNAYVAAKDVEGMEKLLMQME---ADPMATVDWYTYSTAANGY 175
Query: 253 IRNASVDDCLEKAASTLKEMEKRTS-RETRVAYSSLLSLHANMGNVDEVNRIWGKMKACF 311
++ +V EKA + LK+ E+ ++ R+AY SL S +A +GN DEV R+W ++K
Sbjct: 176 LKAGNV----EKALAALKKSEQSVKGKKLRLAYESLQSTYAAIGNNDEVYRLWNRIKN-L 340
Query: 312 CKMSDDEYVCMISSLVKLGDFAG 334
+ Y+CM+SSL+KL D G
Sbjct: 341 PNCWNSSYICMLSSLLKLDDLDG 409
>CN825203
Length = 644
Score = 77.0 bits (188), Expect = 7e-15
Identities = 42/149 (28%), Positives = 78/149 (52%), Gaps = 3/149 (2%)
Frame = -2
Query: 352 RVSNLLLTSYVDQGQMEMAEIFCNQLVEKGVCLSYSSWELLTRGYLKKKDVKKFLHYFGK 411
+V N+LL Y +G +E AE +V+KG + +SW ++ G++ K++++K F +
Sbjct: 643 KVPNILLIGYSRKGLIEKAETMLRSMVDKGKTPTPNSWSIIASGHVAKENMEKAFQCFKE 464
Query: 412 AISSV---KQWIPDPRLVQEAFTVIQEQAHIEGAEQLLVILRNAGHVNTNIYNLFLKTYA 468
A++ + K W P +V + + + IE E + L+ +N ++Y +K Y
Sbjct: 463 ALAVLAENKGWRPKSDVVSSILSWVSDNRDIEEVEDFVNSLKKVMSMNRDMYLSLIKLYV 284
Query: 469 AAGKMPLVVAERMKKDNVQLDKETHRLLD 497
G+ V E MK DN++LD+E +L+
Sbjct: 283 RCGREVDGVLESMKADNIELDEEMEEILN 197
>AU089369
Length = 728
Score = 74.7 bits (182), Expect = 3e-14
Identities = 42/158 (26%), Positives = 84/158 (52%), Gaps = 5/158 (3%)
Frame = +3
Query: 348 TNDVRVSNLLLTSYVDQGQMEMAEIFCNQLVEKGVCLSYSSWELLTRGYLKKKDVKKFLH 407
T D+RV+N+L+ +Y ++ AE + +G + +WE+ +L+K D K +
Sbjct: 18 TYDIRVANVLIGTYAKLDMLDKAEELKERARRRGAKPNAKTWEIFLDYHLRKGDFKLAVD 197
Query: 408 YFGKAISSVK----QWIPDPRLVQEAFTVIQEQAHIEGAEQLLVILRNA-GHVNTNIYNL 462
KAIS + +W+P V +++ ++GA + L IL+ + + +++
Sbjct: 198 SLDKAISIGRGNGDKWVPSSESVGTIMKHFEQEKDVDGAXEFLEILKKSTDSLXADVFEP 377
Query: 463 FLKTYAAAGKMPLVVAERMKKDNVQLDKETHRLLDLTS 500
++TYAAAG+ + R+K +NV++ ++T +LL+ S
Sbjct: 378 LIRTYAAAGRTSSALQRRLKMENVEVREDTXKLLEAIS 491
>AV410889
Length = 436
Score = 70.5 bits (171), Expect = 6e-13
Identities = 40/99 (40%), Positives = 58/99 (58%)
Frame = +1
Query: 29 ASSSSGGDTLGRRLLSLVYPKRSAVIAINKWKEEGHTLPRKYQLNRMIRELRKNKRYKHA 88
+S SS DTL R+ P +N+W +EG + L I++LR +R+KHA
Sbjct: 142 SSYSSPIDTLFLRISRTGDPAIPVTPLLNQWVQEGRPINHG-DLQFFIKQLRSFRRFKHA 318
Query: 89 LEVCEWMKLQNDIKLVEGDYAVQLDLITKVRGLNSAEKF 127
L++ EWM + + L GD A++LDLI+KVRGL AEK+
Sbjct: 319 LQISEWMSDERNHYLHSGDIAIRLDLISKVRGLEEAEKY 435
>TC10842
Length = 597
Score = 57.0 bits (136), Expect = 7e-09
Identities = 29/117 (24%), Positives = 63/117 (53%)
Frame = +2
Query: 266 ASTLKEMEKRTSRETRVAYSSLLSLHANMGNVDEVNRIWGKMKACFCKMSDDEYVCMISS 325
+++L E EKR ++ + Y L+ L+A +G+ D++++IW ++ KM + Y C+IS+
Sbjct: 2 SNSLAETEKRITQSQWITYDFLIILYAGLGSKDKLDQIWNSLRMTKQKMINRNYSCIISA 181
Query: 326 LVKLGDFAGVENLYKEWESVSGTNDVRVSNLLLTSYVDQGQMEMAEIFCNQLVEKGV 382
+ LG V + +W++ + D+ ++ ++ G ++A L+EK +
Sbjct: 182 YLMLGHVKEVGEVIDQWKNSTIDFDMLACKKIMDAFRATGLADIANNLNMILIEKNI 352
>AV429083
Length = 316
Score = 49.3 bits (116), Expect = 1e-06
Identities = 27/55 (49%), Positives = 34/55 (61%)
Frame = +3
Query: 198 ELKVNTSPDVVTFNLLLTACASENDVETAERVLLQLKKAKVDPDWVTYSTLTNLY 252
ELK N PDV TFN+L+ A + V+ A+ VL + K V PD VTYSTL + Y
Sbjct: 18 ELKNNIKPDVSTFNILVDALCKKGKVKHAKNVLAVMIKQGVAPDLVTYSTLLDGY 182
>AV427280
Length = 268
Score = 45.8 bits (107), Expect = 2e-05
Identities = 23/76 (30%), Positives = 47/76 (61%), Gaps = 1/76 (1%)
Frame = +3
Query: 161 MSKMSECGFLRSPVPYNRMMSLYISNGKLEKVPKLFEELKVN-TSPDVVTFNLLLTACAS 219
M KM E G++ S + YN +M LY + + EKVP + +K + SPD+ ++ + + + +
Sbjct: 18 MQKMKEMGYV-SCLNYNNIMCLYAQSDQHEKVPGVLAMMKEDGVSPDIFSYRVCINSYGA 194
Query: 220 ENDVETAERVLLQLKK 235
+D+E E++L ++++
Sbjct: 195 RSDLENMEKLLEEIER 242
>CB826792
Length = 485
Score = 45.4 bits (106), Expect = 2e-05
Identities = 39/162 (24%), Positives = 66/162 (40%), Gaps = 2/162 (1%)
Frame = +3
Query: 184 ISNGKL--EKVPKLFEELKVNTSPDVVTFNLLLTACASENDVETAERVLLQLKKAKVDPD 241
++ G+L E V L K N +PDVVT+N L+ ++ A+ V + K + P
Sbjct: 3 LAKGRLVKEAVNLLQTMEKNNVTPDVVTYNSLIKPLCKNRKIDEAKEVFNDMMKRNITPT 182
Query: 242 WVTYSTLTNLYIRNASVDDCLEKAASTLKEMEKRTSRETRVAYSSLLSLHANMGNVDEVN 301
T+ + V + L+K ++E+ + ET Y L+ +DEV
Sbjct: 183 IRTFHAFFRILRVEEEVFELLDK----MRELGCYPTIET---YIMLIRKFCRWRKLDEVF 341
Query: 302 RIWGKMKACFCKMSDDEYVCMISSLVKLGDFAGVENLYKEWE 343
+IW M+ Y+ +I L G + Y E +
Sbjct: 342 KIWNMMREDGVSHDRSSYIVLIHGLFLNGKVKEAHDYYIEMQ 467
>AV417736
Length = 428
Score = 40.4 bits (93), Expect = 7e-04
Identities = 30/135 (22%), Positives = 67/135 (49%), Gaps = 4/135 (2%)
Frame = +1
Query: 122 NSAEKFFEDLPDKMRGQPTCTA---LLHAYVQNNLTNKAEALMSKMSECGFLRSPVPYNR 178
++ E+ F D+ K +P A L YV++ KAEAL++ M + G + V +
Sbjct: 1 DNCEEIFNDMV-KAGIEPDIHAYSILAKGYVRSGQPGKAEALLTSMRKYGLQPNVVIFTT 177
Query: 179 MMSLYISNGKLEKVPKLFEEL-KVNTSPDVVTFNLLLTACASENDVETAERVLLQLKKAK 237
++S + + GK+++ +L E++ ++ ++ T+ L+ AE +L+ +++
Sbjct: 178 IISGWCTAGKMDRAVRLHEKMHEMGIPSNLKTYETLMWGYGEAKQPWKAEELLVTMEERG 357
Query: 238 VDPDWVTYSTLTNLY 252
V P+ T + + +
Sbjct: 358 VAPEMSTMQLVADAW 402
Score = 34.7 bits (78), Expect = 0.038
Identities = 22/84 (26%), Positives = 38/84 (45%)
Frame = +1
Query: 224 ETAERVLLQLKKAKVDPDWVTYSTLTNLYIRNASVDDCLEKAASTLKEMEKRTSRETRVA 283
+ E + + KA ++PD YS L Y+R+ KA + L M K + V
Sbjct: 1 DNCEEIFNDMVKAGIEPDIHAYSILAKGYVRSGQPG----KAEALLTSMRKYGLQPNVVI 168
Query: 284 YSSLLSLHANMGNVDEVNRIWGKM 307
+++++S G +D R+ KM
Sbjct: 169 FTTIISGWCTAGKMDRAVRLHEKM 240
>AV421795
Length = 267
Score = 39.7 bits (91), Expect = 0.001
Identities = 23/76 (30%), Positives = 40/76 (52%)
Frame = +2
Query: 29 ASSSSGGDTLGRRLLSLVYPKRSAVIAINKWKEEGHTLPRKYQLNRMIRELRKNKRYKHA 88
+SSSS D+L R+ +N+W +EG + +L I++LR ++R+ HA
Sbjct: 41 SSSSSPTDSLFLRISRSGDRAIPMTPLLNQWLQEGKLINYS-ELQFFIKQLRASRRFNHA 217
Query: 89 LEVCEWMKLQNDIKLV 104
L++ EWM + L+
Sbjct: 218 LQISEWMSNERKFHLL 265
>TC15322 weakly similar to UP|Q9LG23 (Q9LG23) F14J16.14
(At1g55890/F14J16_4), partial (33%)
Length = 588
Score = 37.7 bits (86), Expect = 0.005
Identities = 21/77 (27%), Positives = 43/77 (55%), Gaps = 2/77 (2%)
Frame = +2
Query: 178 RMMSLYISNGKLEKVPKLFEEL-KVNTSPDVVTFNLLLTACASENDVETAERVLLQLK-K 235
R+++LY +G ++ ++F+E+ + N + V++FN LL A ER+ ++ +
Sbjct: 356 RLITLYGKSGMPKRARQMFDEMPERNCTRTVLSFNALLAAYLHSKQYRVVERIFKEVPGE 535
Query: 236 AKVDPDWVTYSTLTNLY 252
V PD V+Y+T+ +
Sbjct: 536 ISVKPDLVSYNTVIKAF 586
>AV767789
Length = 560
Score = 37.4 bits (85), Expect = 0.006
Identities = 17/55 (30%), Positives = 30/55 (53%)
Frame = -1
Query: 200 KVNTSPDVVTFNLLLTACASENDVETAERVLLQLKKAKVDPDWVTYSTLTNLYIR 254
K+ P V+T+N+L+ A A ++L ++ + PD VTYST+ ++R
Sbjct: 518 KIGLHPTVMTYNMLMNAYARGGQHSKLPQLLKEMAALNLKPDSVTYSTMIYAFVR 354
>BP044414
Length = 536
Score = 35.8 bits (81), Expect = 0.017
Identities = 30/128 (23%), Positives = 58/128 (44%)
Frame = -1
Query: 264 KAASTLKEMEKRTSRETRVAYSSLLSLHANMGNVDEVNRIWGKMKACFCKMSDDEYVCMI 323
+A L+EMEK+ + + ++++ N GN+DE + +G+++ + + ++
Sbjct: 536 EAVEFLQEMEKKDVKPDLFSLNAIIKGFVNEGNLDEAKKWFGEIEKSENDPDKNTFATLV 357
Query: 324 SSLVKLGDFAGVENLYKEWESVSGTNDVRVSNLLLTSYVDQGQMEMAEIFCNQLVEKGVC 383
L + GD ++ KE + T RV LL VD+ E ++VE G
Sbjct: 356 PFLCEKGDLKTAIDVVKE---IFNTR-CRVGAPLLQLVVDKLVSESMLSEAKEIVELGKT 189
Query: 384 LSYSSWEL 391
Y ++L
Sbjct: 188 NRYCHYKL 165
Score = 34.7 bits (78), Expect = 0.038
Identities = 17/59 (28%), Positives = 35/59 (58%)
Frame = -1
Query: 190 EKVPKLFEELKVNTSPDVVTFNLLLTACASENDVETAERVLLQLKKAKVDPDWVTYSTL 248
E V L E K + PD+ + N ++ +E +++ A++ +++K++ DPD T++TL
Sbjct: 536 EAVEFLQEMEKKDVKPDLFSLNAIIKGFVNEGNLDEAKKWFGEIEKSENDPDKNTFATL 360
>BI418759
Length = 543
Score = 35.0 bits (79), Expect = 0.029
Identities = 30/139 (21%), Positives = 58/139 (41%), Gaps = 15/139 (10%)
Frame = +2
Query: 140 TCTALLHAYVQNNLTNKAEALMSKMSECGFLRSPVP----------YNRMMSLYISNGKL 189
TC LL +++ +A AL +M L + P +N M++ + GK
Sbjct: 119 TCNVLLEVLLKHGKKTQAWALFDQM-----LDNHTPPNFQAVNSDTFNIMVNECFNLGKF 283
Query: 190 EKVPKLFEELKVNTSP-----DVVTFNLLLTACASENDVETAERVLLQLKKAKVDPDWVT 244
+ F + ++ DV +N +++ + AE + +L + PD T
Sbjct: 284 HEALATFRKAGTKSNSKAFAMDVAGYNNIISRFCENGLLSEAETLFEELCSKSLSPDVPT 463
Query: 245 YSTLTNLYIRNASVDDCLE 263
+ TL + Y++ +DD L+
Sbjct: 464 HRTLIDAYMKMERIDDALK 520
>TC13348 weakly similar to UP|Q9LUJ4 (Q9LUJ4) Gb|AAF26800.1, partial (7%)
Length = 539
Score = 33.9 bits (76), Expect = 0.065
Identities = 15/46 (32%), Positives = 27/46 (58%)
Frame = +3
Query: 206 DVVTFNLLLTACASENDVETAERVLLQLKKAKVDPDWVTYSTLTNL 251
DVVT+N +++ + + ETA R+L ++++ PD TY L +
Sbjct: 33 DVVTYNTMISTACAHSREETALRLLKEMEERSCTPDLETYHPLLKM 170
>BU494388
Length = 350
Score = 33.5 bits (75), Expect = 0.085
Identities = 20/77 (25%), Positives = 40/77 (50%), Gaps = 4/77 (5%)
Frame = +2
Query: 154 TNKAEALMSKMSECGFLRSPVPYNRMMSLYISNGKLEKVP---KLFEELK-VNTSPDVVT 209
++KA ++ + E + ++ L + GKL+ +P ++F ++ + P +
Sbjct: 62 SDKARLVLEWILEKMLKENVKDHSLFSELILLCGKLKNLPLGMRVFTSMETIGVKPSSLV 241
Query: 210 FNLLLTACASENDVETA 226
FN L++AC S ND+ TA
Sbjct: 242 FNSLISACLSSNDIVTA 292
>AV779633
Length = 440
Score = 33.5 bits (75), Expect = 0.085
Identities = 27/117 (23%), Positives = 51/117 (43%)
Frame = -2
Query: 144 LLHAYVQNNLTNKAEALMSKMSECGFLRSPVPYNRMMSLYISNGKLEKVPKLFEELKVNT 203
L+ Y + L ++A ALM + G V YN ++ + G L + LF+E+ +
Sbjct: 397 LIDGYCEAGLMSQALALMENSWKTGVKPDIVSYNSLLKGFCKAGDLVRAESLFDEI-LGF 221
Query: 204 SPDVVTFNLLLTACASENDVETAERVLLQLKKAKVDPDWVTYSTLTNLYIRNASVDD 260
D + L N V+T + + + P TY+TL + Y ++ +++
Sbjct: 220 QRDGESGQL------KNNAVDTRDEL------RNIRPTLATYTTLISAYGKHCGIEE 86
>BF177625
Length = 284
Score = 33.1 bits (74), Expect = 0.11
Identities = 17/63 (26%), Positives = 30/63 (46%)
Frame = +1
Query: 160 LMSKMSECGFLRSPVPYNRMMSLYISNGKLEKVPKLFEELKVNTSPDVVTFNLLLTACAS 219
L +M + GF + + YN M+ ++ KV +L+ K DV+T+N ++ A
Sbjct: 73 LFDEMLQRGFAPNTITYNVMLDVFGKAKLFRKVRRLYFMAKKQGLVDVITYNTIIAAYGK 252
Query: 220 END 222
D
Sbjct: 253 NKD 261
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.317 0.132 0.378
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,220,056
Number of Sequences: 28460
Number of extensions: 107303
Number of successful extensions: 557
Number of sequences better than 10.0: 50
Number of HSP's better than 10.0 without gapping: 537
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 547
length of query: 512
length of database: 4,897,600
effective HSP length: 94
effective length of query: 418
effective length of database: 2,222,360
effective search space: 928946480
effective search space used: 928946480
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 57 (26.6 bits)
Medicago: description of AC122725.14