BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC122724.2 + phase: 0
(255 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC10877 similar to UP|O22840 (O22840) At2g43630 protein, partial... 139 6e-34
TC9245 120 2e-28
BP051645 56 5e-09
TC12067 46 5e-06
TC14758 similar to UP|O24601 (O24601) Glycine-rich RNA binding p... 37 0.003
TC11992 similar to UP|Q9QY28 (Q9QY28) Espin (Fragment), partial ... 35 0.010
TC19911 weakly similar to PIR|A44805|A44805 eggshell protein pre... 34 0.021
TC9801 homologue to UP|Q8LAC6 (Q8LAC6) Glycine rich protein-like... 34 0.021
TC18522 weakly similar to UP|HSP1_OCTVU (P83214) Sperm protamine... 34 0.028
TC20112 weakly similar to UP|Q9SBM1 (Q9SBM1) Hydroxyproline-rich... 34 0.028
TC15380 similar to UP|Q8H5P5 (Q8H5P5) OJ1003_C06.27 protein, par... 33 0.037
BP030058 33 0.037
TC16065 weakly similar to UP|Q9SBM1 (Q9SBM1) Hydroxyproline-rich... 33 0.062
TC14984 similar to GB|AAM62447.1|21553354|AY085214 glycine-rich ... 33 0.062
TC17912 weakly similar to GB|AAP37829.1|30725614|BT008470 At5g12... 33 0.062
BP084209 32 0.082
TC10062 similar to BAD08700 (BAD08700) Cold shock domain protein... 32 0.082
TC15523 similar to UP|GRPA_MAIZE (P10979) Glycine-rich RNA-bindi... 32 0.11
BI419822 32 0.14
CB829112 32 0.14
>TC10877 similar to UP|O22840 (O22840) At2g43630 protein, partial (17%)
Length = 629
Score = 139 bits (349), Expect = 6e-34
Identities = 85/194 (43%), Positives = 111/194 (56%), Gaps = 16/194 (8%)
Frame = +2
Query: 1 MNAIQVAASNPAIYVRKNYQPHHFLSTPKGLSMNANPLSRTFLIPRAKLSSGLYCVRQSA 60
MN QV A P I V+ Q H +TP L MN +P SR + R K S G C+
Sbjct: 110 MNTAQVTAWQPGICVKNISQLHQLDTTPCILPMNTHPRSRIVMKLREKPSFGFKCL---- 277
Query: 61 TTAPVLKVYPSNLTQSVPVLKSQKPRHVCLAGGKGMM-DNSEDSQRKSLEEAMKS----- 114
P+LKS+KP +C AGG+GMM DN E+S KS+E+AM+
Sbjct: 278 -----------------PILKSRKPLRICFAGGEGMMGDNDENSPWKSIEKAMEKFKGQS 406
Query: 115 ----LQEKIQKGEY--SGGSGSKPPGGRGRGGGGGNS----DGSEEGSTGGMSDETAQIV 164
L+++IQKGEY +GGSG+KPPGG G GGGGG+S DG EE S M DE Q++
Sbjct: 407 IEDVLRQQIQKGEYFDNGGSGAKPPGGSGGGGGGGDSPDETDGPEEESFAEMLDENLQVI 586
Query: 165 FATIGFMFVYIYVI 178
AT+GF+F+YIY++
Sbjct: 587 LATLGFIFLYIYIL 628
>TC9245
Length = 583
Score = 120 bits (301), Expect = 2e-28
Identities = 60/101 (59%), Positives = 78/101 (76%), Gaps = 1/101 (0%)
Frame = +1
Query: 155 GMSDETAQIVFATIGFMFVYIYVINGVELTKLARDFIKYLLGGTQSVRLKRASYKCSRFY 214
G+SDE QI+ ATIGF+ VYI VI+GVEL KL++D +KYL GGTQS+RLK+A YK +RF+
Sbjct: 7 GLSDEIQQIILATIGFILVYICVIDGVELAKLSKDILKYLFGGTQSLRLKKAIYKWTRFF 186
Query: 215 KKITGQNEVDEDGLETGPLRWNIADFYR-DVLRNYMKPNSN 254
K +TG +V ++ LE P + N D+Y DVLRNYMKPNS+
Sbjct: 187 KLLTGNRKVLKNELEEAPTQRNNLDYYHDDVLRNYMKPNSD 309
>BP051645
Length = 456
Score = 56.2 bits (134), Expect = 5e-09
Identities = 32/66 (48%), Positives = 39/66 (58%), Gaps = 4/66 (6%)
Frame = -2
Query: 186 LARDFIKYLLGGTQSVRLKRASYKCSRFYKKITGQNEVDEDGLETG----PLRWNIADFY 241
LARD+IKYL GG+QSVRLK A K Y+ +T + EVDE LE P W +
Sbjct: 455 LARDYIKYLFGGSQSVRLKTAMVKWGPIYRSMTTKKEVDEFWLERAIMNTPTWWYDPEDL 276
Query: 242 RDVLRN 247
R+ LRN
Sbjct: 275 REALRN 258
>TC12067
Length = 410
Score = 46.2 bits (108), Expect = 5e-06
Identities = 34/81 (41%), Positives = 39/81 (47%)
Frame = +1
Query: 7 AASNPAIYVRKNYQPHHFLSTPKGLSMNANPLSRTFLIPRAKLSSGLYCVRQSATTAPVL 66
+A P I V+ Q HHF S P SMNA PLSRT L P A+ + GL P L
Sbjct: 229 SARKPVIPVKNKSQSHHFTSLPCISSMNAFPLSRTSLRPGARPAFGL----------PYL 378
Query: 67 KVYPSNLTQSVPVLKSQKPRH 87
PVLKSQ+P H
Sbjct: 379 -----------PVLKSQQPFH 408
>TC14758 similar to UP|O24601 (O24601) Glycine-rich RNA binding protein 2,
partial (93%)
Length = 725
Score = 37.4 bits (85), Expect = 0.003
Identities = 19/39 (48%), Positives = 22/39 (55%), Gaps = 3/39 (7%)
Frame = +1
Query: 120 QKGEYSGGSGSKPPGGRG---RGGGGGNSDGSEEGSTGG 155
++G Y GG GS+ GG G GGGGG G EG GG
Sbjct: 382 REGGYGGGGGSRGYGGGGGGYGGGGGGGYGGRREGGYGG 498
Score = 33.9 bits (76), Expect = 0.028
Identities = 18/34 (52%), Positives = 19/34 (54%)
Frame = +1
Query: 122 GEYSGGSGSKPPGGRGRGGGGGNSDGSEEGSTGG 155
G Y GG G GGR GG GG+ GS GS GG
Sbjct: 436 GGYGGGGGGGY-GGRREGGYGGDGGGSRYGSGGG 534
Score = 33.1 bits (74), Expect = 0.048
Identities = 21/61 (34%), Positives = 29/61 (47%), Gaps = 5/61 (8%)
Frame = +1
Query: 100 SEDSQRKSLE----EAMKSLQEKIQKGEYSGGSGSKPPGGRGRGGGG-GNSDGSEEGSTG 154
SE++ R ++E + + + + GG G GG GRGGGG G G EG G
Sbjct: 232 SEEAMRSAIEGMNGNELDGRNITVNEAQARGGGG----GGGGRGGGGYGGGGGRREGGYG 399
Query: 155 G 155
G
Sbjct: 400 G 402
Score = 26.2 bits (56), Expect = 5.8
Identities = 13/25 (52%), Positives = 15/25 (60%)
Frame = +1
Query: 120 QKGEYSGGSGSKPPGGRGRGGGGGN 144
++G Y GG G G G GGGGGN
Sbjct: 478 REGGY-GGDGGGSRYGSGGGGGGGN 549
>TC11992 similar to UP|Q9QY28 (Q9QY28) Espin (Fragment), partial (4%)
Length = 560
Score = 35.4 bits (80), Expect = 0.010
Identities = 20/55 (36%), Positives = 28/55 (50%)
Frame = -3
Query: 115 LQEKIQKGEYSGGSGSKPPGGRGRGGGGGNSDGSEEGSTGGMSDETAQIVFATIG 169
L E++Q ++GG G G GRGGGGG G +E + G S + F +G
Sbjct: 363 LPERLQLS-FAGGDGGD---GVGRGGGGGGGGGGDESKSDGGSGNSNDSSFGILG 211
>TC19911 weakly similar to PIR|A44805|A44805 eggshell protein precursor -
fluke (Schistosoma haematobium) {Schistosoma
haematobium;} , partial (24%)
Length = 350
Score = 34.3 bits (77), Expect = 0.021
Identities = 17/37 (45%), Positives = 19/37 (50%)
Frame = +1
Query: 122 GEYSGGSGSKPPGGRGRGGGGGNSDGSEEGSTGGMSD 158
G +GG G GGR GGGGG DG +GG D
Sbjct: 193 GHSAGGYGDH--GGRSGGGGGGYGDGGLSSGSGGYGD 297
>TC9801 homologue to UP|Q8LAC6 (Q8LAC6) Glycine rich protein-like, complete
Length = 981
Score = 34.3 bits (77), Expect = 0.021
Identities = 24/74 (32%), Positives = 31/74 (41%), Gaps = 16/74 (21%)
Frame = +2
Query: 129 GSKPPGGRGRGGGGGNSDGSEEGSTG----------------GMSDETAQIVFATIGFMF 172
G+K PGGRGRGG GG GS G +D TA I F F
Sbjct: 461 GAKGPGGRGRGGLGGKPGGSRGAGRGRG*FLQVMRSSCLSCVPAADFTAPIFTVHP*FYF 640
Query: 173 VYIYVINGVELTKL 186
++++ LTK+
Sbjct: 641 PNVFLVLMASLTKI 682
Score = 27.3 bits (59), Expect = 2.6
Identities = 18/39 (46%), Positives = 23/39 (58%), Gaps = 1/39 (2%)
Frame = +2
Query: 117 EKIQKGEYSGGSGSKPPG-GRGRGGGGGNSDGSEEGSTG 154
+K+Q+ E + KPPG GRGRG G G EEG+ G
Sbjct: 326 DKVQE-ETKSRTDRKPPGVGRGRGRG-----GREEGAGG 424
Score = 26.6 bits (57), Expect = 4.5
Identities = 15/41 (36%), Positives = 18/41 (43%), Gaps = 6/41 (14%)
Frame = +2
Query: 121 KGEYSGGSGSKPPGGRGRG------GGGGNSDGSEEGSTGG 155
+G G+G +P G GRG G GG G G GG
Sbjct: 395 RGGREEGAGGRPAKGPGRGFDDGAKGPGGRGRGGLGGKPGG 517
>TC18522 weakly similar to UP|HSP1_OCTVU (P83214) Sperm protamine P1 (Po1)
[Contains: Sperm protamine P2 (Po2) (Main protamine)],
partial (68%)
Length = 320
Score = 33.9 bits (76), Expect = 0.028
Identities = 23/79 (29%), Positives = 34/79 (42%)
Frame = -2
Query: 92 GGKGMMDNSEDSQRKSLEEAMKSLQEKIQKGEYSGGSGSKPPGGRGRGGGGGNSDGSEEG 151
G +G + + R LEE +S+ Q+GE SGG GGGG + EG
Sbjct: 310 GARGGRHRNRNGNRSLLEEVRRSVVVSRQRGEDSGGE------TLAAVGGGGGFEEVVEG 149
Query: 152 STGGMSDETAQIVFATIGF 170
+ DE + A +G+
Sbjct: 148 ENDVVFDEVVVVGGAVLGW 92
>TC20112 weakly similar to UP|Q9SBM1 (Q9SBM1) Hydroxyproline-rich
glycoprotein DZ-HRGP precursor, partial (11%)
Length = 523
Score = 33.9 bits (76), Expect = 0.028
Identities = 19/41 (46%), Positives = 20/41 (48%), Gaps = 1/41 (2%)
Frame = +2
Query: 118 KIQKGEYSGGSGSKPPGGRG-RGGGGGNSDGSEEGSTGGMS 157
K G YSGG G + GG RGGG G G G GG S
Sbjct: 398 KYDTGRYSGGGGGRGRGGGSIRGGGRGGRGGGFGGRHGGGS 520
>TC15380 similar to UP|Q8H5P5 (Q8H5P5) OJ1003_C06.27 protein, partial (28%)
Length = 1306
Score = 33.5 bits (75), Expect = 0.037
Identities = 21/74 (28%), Positives = 33/74 (44%), Gaps = 3/74 (4%)
Frame = -2
Query: 88 VCLAGGKGMMDNSEDSQRKSLEEAMKSLQ---EKIQKGEYSGGSGSKPPGGRGRGGGGGN 144
V + GG+ ++D +D + E A ++ + +GE S GRG GGGGG
Sbjct: 477 VTVLGGETVVDGDDDGVEGTAELAADGVELDRRRRHEGETSAVEEHNEGKGRGGGGGGGG 298
Query: 145 SDGSEEGSTGGMSD 158
+ E + G D
Sbjct: 297 KEAEPEVAGGVHGD 256
>BP030058
Length = 455
Score = 33.5 bits (75), Expect = 0.037
Identities = 20/46 (43%), Positives = 23/46 (49%)
Frame = +1
Query: 113 KSLQEKIQKGEYSGGSGSKPPGGRGRGGGGGNSDGSEEGSTGGMSD 158
K LQ Q+G + GG GG GGGGG+ GS G GG D
Sbjct: 262 KGLQFCGQEGYFGGGGEGGGGGGGDGGGGGGDGHGS--GGGGGEGD 393
>TC16065 weakly similar to UP|Q9SBM1 (Q9SBM1) Hydroxyproline-rich
glycoprotein DZ-HRGP precursor, partial (20%)
Length = 608
Score = 32.7 bits (73), Expect = 0.062
Identities = 15/29 (51%), Positives = 15/29 (51%)
Frame = -1
Query: 127 GSGSKPPGGRGRGGGGGNSDGSEEGSTGG 155
G G P GGRG G GGG G G GG
Sbjct: 338 GYGGPPQGGRGGGYGGGGGPGYGGGGRGG 252
Score = 32.3 bits (72), Expect = 0.082
Identities = 18/35 (51%), Positives = 19/35 (53%)
Frame = -1
Query: 121 KGEYSGGSGSKPPGGRGRGGGGGNSDGSEEGSTGG 155
+G YSGG G GG GRGGGG S S GG
Sbjct: 170 RGGYSGGGGGY-GGGGGRGGGGMGSGRGVSPSHGG 69
Score = 26.6 bits (57), Expect = 4.5
Identities = 11/24 (45%), Positives = 14/24 (57%)
Frame = -1
Query: 132 PPGGRGRGGGGGNSDGSEEGSTGG 155
PP +GRG GG + G G +GG
Sbjct: 221 PPEYQGRGRGGPSQQGGRGGYSGG 150
Score = 26.6 bits (57), Expect = 4.5
Identities = 13/30 (43%), Positives = 13/30 (43%)
Frame = -1
Query: 126 GGSGSKPPGGRGRGGGGGNSDGSEEGSTGG 155
G G GGRG GGG G G GG
Sbjct: 200 GRGGPSQQGGRGGYSGGGGGYGGGGGRGGG 111
>TC14984 similar to GB|AAM62447.1|21553354|AY085214 glycine-rich RNA binding
protein 7 {Arabidopsis thaliana;} , partial (19%)
Length = 483
Score = 32.7 bits (73), Expect = 0.062
Identities = 17/37 (45%), Positives = 21/37 (55%), Gaps = 1/37 (2%)
Frame = -3
Query: 120 QKGEYSGGSGSKPPGGRG-RGGGGGNSDGSEEGSTGG 155
++GE SGG G + PGG G GGGG + G GG
Sbjct: 127 REGE*SGGGGRRAPGGGGWVDGGGGRRPSAVVGVAGG 17
Score = 27.3 bits (59), Expect = 2.6
Identities = 13/32 (40%), Positives = 16/32 (49%)
Frame = -1
Query: 120 QKGEYSGGSGSKPPGGRGRGGGGGNSDGSEEG 151
+ GE +G G GRGGGGG +G G
Sbjct: 222 EDGELFNVAGDDGISGGGRGGGGGGFEGCGGG 127
Score = 25.4 bits (54), Expect = 10.0
Identities = 13/28 (46%), Positives = 14/28 (49%), Gaps = 2/28 (7%)
Frame = -1
Query: 134 GGRGRGGGG--GNSDGSEEGSTGGMSDE 159
GGRG GGGG G G E G + E
Sbjct: 174 GGRGGGGGGFEGCGGGERESEAGAVVGE 91
>TC17912 weakly similar to GB|AAP37829.1|30725614|BT008470 At5g12470
{Arabidopsis thaliana;}, partial (18%)
Length = 507
Score = 32.7 bits (73), Expect = 0.062
Identities = 17/52 (32%), Positives = 23/52 (43%)
Frame = +3
Query: 124 YSGGSGSKPPGGRGRGGGGGNSDGSEEGSTGGMSDETAQIVFATIGFMFVYI 175
+S G+G GG GGGGG G E + A +V A +G Y+
Sbjct: 216 FSAGNGGGGTGGGDGGGGGGGGGGDGESEDRDRNRGEALLVLAEVGKPLEYL 371
>BP084209
Length = 502
Score = 32.3 bits (72), Expect = 0.082
Identities = 14/48 (29%), Positives = 24/48 (49%)
Frame = -3
Query: 98 DNSEDSQRKSLEEAMKSLQEKIQKGEYSGGSGSKPPGGRGRGGGGGNS 145
D+ +D ++S++ K + ++G + GRGRGGGG S
Sbjct: 449 DDDDDEMPNRKRRGVQSVEPKPHRSNFNGRGRGRGGRGRGRGGGGRGS 306
>TC10062 similar to BAD08700 (BAD08700) Cold shock domain protein 2, partial
(66%)
Length = 481
Score = 32.3 bits (72), Expect = 0.082
Identities = 17/35 (48%), Positives = 17/35 (48%), Gaps = 1/35 (2%)
Frame = +3
Query: 122 GEYSGGSGSKPPGGRGRGGG-GGNSDGSEEGSTGG 155
G Y GG G GG G GGG GG G G GG
Sbjct: 372 GGYGGGGGYGGSGGYGGGGGYGGGGRGGGRGGGGG 476
Score = 32.0 bits (71), Expect = 0.11
Identities = 22/70 (31%), Positives = 32/70 (45%), Gaps = 3/70 (4%)
Frame = +3
Query: 89 CLAGGKGM---MDNSEDSQRKSLEEAMKSLQEKIQKGEYSGGSGSKPPGGRGRGGGGGNS 145
CL G+ + + + D + K++E + Q +Q GG+G GG G GGGG
Sbjct: 165 CLGEGEDVEFIVASDPDGRAKAIEVTGPNGQA-VQGTRRGGGAGGGGRGGGGYGGGGYGG 341
Query: 146 DGSEEGSTGG 155
G G GG
Sbjct: 342 GGYGGGGRGG 371
Score = 30.4 bits (67), Expect = 0.31
Identities = 16/33 (48%), Positives = 16/33 (48%)
Frame = +3
Query: 122 GEYSGGSGSKPPGGRGRGGGGGNSDGSEEGSTG 154
G GG G GG GRGGGG G GS G
Sbjct: 315 GYGGGGYGGGGYGGGGRGGGGYGGGGGYGGSGG 413
Score = 26.6 bits (57), Expect = 4.5
Identities = 19/39 (48%), Positives = 19/39 (48%), Gaps = 5/39 (12%)
Frame = +3
Query: 122 GEYSGGS---GSKPPGGRGRGG--GGGNSDGSEEGSTGG 155
G Y GG G GGRG GG GGG GS G GG
Sbjct: 312 GGYGGGGYGGGGYGGGGRGGGGYGGGGGYGGS--GGYGG 422
Score = 25.4 bits (54), Expect = 10.0
Identities = 13/24 (54%), Positives = 13/24 (54%), Gaps = 3/24 (12%)
Frame = +3
Query: 122 GEYSGGSG---SKPPGGRGRGGGG 142
G Y GG G GGRG GGGG
Sbjct: 408 GGYGGGGGYGGGGRGGGRGGGGGG 479
>TC15523 similar to UP|GRPA_MAIZE (P10979) Glycine-rich RNA-binding,
abscisic acid-inducible protein, complete
Length = 972
Score = 32.0 bits (71), Expect = 0.11
Identities = 17/29 (58%), Positives = 17/29 (58%)
Frame = +2
Query: 126 GGSGSKPPGGRGRGGGGGNSDGSEEGSTG 154
GGSG GGR GGGGG GS EG G
Sbjct: 704 GGSGGGGYGGRREGGGGGYG-GSREGGGG 787
Score = 27.3 bits (59), Expect = 2.6
Identities = 18/51 (35%), Positives = 23/51 (44%), Gaps = 4/51 (7%)
Frame = +2
Query: 109 EEAMKSLQEKIQKGEYSGGS---GSKPPGGRGRGGGGGN-SDGSEEGSTGG 155
E++MK E + + G + GRG GGGGG G EG GG
Sbjct: 509 EQSMKDAIEGMNGQDIDGRNVTVNEAQARGRGGGGGGGGYGGGRREGGYGG 661
>BI419822
Length = 380
Score = 31.6 bits (70), Expect = 0.14
Identities = 20/56 (35%), Positives = 27/56 (47%)
Frame = +2
Query: 88 VCLAGGKGMMDNSEDSQRKSLEEAMKSLQEKIQKGEYSGGSGSKPPGGRGRGGGGG 143
V A + M D E ++L+ ++ E +G SGG G GG G GGGGG
Sbjct: 215 VTFASEQAMKDAIEAMNGQNLDGRNITVNEAQSRGS-SGGGGRYGGGGGGYGGGGG 379
>CB829112
Length = 234
Score = 31.6 bits (70), Expect = 0.14
Identities = 15/28 (53%), Positives = 15/28 (53%)
Frame = -3
Query: 122 GEYSGGSGSKPPGGRGRGGGGGNSDGSE 149
G GG G GGR GGGGG S SE
Sbjct: 139 GGGGGGGGGSSGGGRRSGGGGGASGASE 56
Score = 26.9 bits (58), Expect = 3.4
Identities = 11/21 (52%), Positives = 12/21 (56%)
Frame = -3
Query: 135 GRGRGGGGGNSDGSEEGSTGG 155
G G GGGGG+S G GG
Sbjct: 139 GGGGGGGGGSSGGGRRSGGGG 77
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.314 0.133 0.383
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,919,963
Number of Sequences: 28460
Number of extensions: 49792
Number of successful extensions: 825
Number of sequences better than 10.0: 160
Number of HSP's better than 10.0 without gapping: 627
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 764
length of query: 255
length of database: 4,897,600
effective HSP length: 88
effective length of query: 167
effective length of database: 2,393,120
effective search space: 399651040
effective search space used: 399651040
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 54 (25.4 bits)
Medicago: description of AC122724.2