BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC122722.6 + phase: 0
(461 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC17041 homologue to GB|AAL25568.1|16648753|AY058152 AT5g16150/T... 113 8e-26
BP068551 93 1e-19
TC10790 homologue to UP|Q8GTR0 (Q8GTR0) Sugar transporter, parti... 80 9e-16
TC9159 similar to UP|HXT4_YEAST (P32467) Low-affinity glucose tr... 68 3e-12
TC10135 66 1e-11
TC9536 similar to UP|Q8LPQ8 (Q8LPQ8) AT4g35300/F23E12_140, parti... 64 5e-11
BG662351 61 4e-10
TC10748 similar to UP|Q7XA50 (Q7XA50) Sorbitol-like transporter,... 57 6e-09
TC16378 similar to GB|AAM70554.1|21700861|AY124845 At1g75220/F22... 55 2e-08
TC8113 weakly similar to UP|O23213 (O23213) Sugar transporter li... 51 5e-07
TC12545 similar to UP|Q7XA50 (Q7XA50) Sorbitol-like transporter,... 48 3e-06
TC12303 similar to GB|CAB10424.1|2245004|ATFCA6 membrane transpo... 48 3e-06
BP053600 47 7e-06
BP074036 46 1e-05
BI420004 45 2e-05
TC19595 45 3e-05
CB829587 40 8e-04
BI418774 39 0.002
TC11822 similar to UP|P93051 (P93051) ORFc protein (Fragment), p... 37 0.005
TC16504 weakly similar to UP|Q84KI7 (Q84KI7) Sorbitol transporte... 37 0.007
>TC17041 homologue to GB|AAL25568.1|16648753|AY058152 AT5g16150/T21H19_70
{Arabidopsis thaliana;} , partial (18%)
Length = 632
Score = 113 bits (282), Expect = 8e-26
Identities = 52/96 (54%), Positives = 73/96 (75%)
Frame = +2
Query: 362 LLFVFTFALGAGPVPGLLLTEIFPSRIRAKAMAFCMSVHWVINFFVGLLFLRLLEKLGAQ 421
+L+V +F+LGAGPVP LLL EIF SRIRAKA++ + +HW+ NF +GL FL ++ K G
Sbjct: 5 VLYVLSFSLGAGPVPALLLPEIFASRIRAKAVSLSLGMHWISNFVIGLYFLSVVNKFGIS 184
Query: 422 LLYSMFATFCIMAVIFVKRNVVETKGKSLQEIEIAL 457
+Y F+ C++AV+++ NVVETKG+SL+EIE AL
Sbjct: 185 SVYLGFSAVCVLAVLYIAGNVVETKGRSLEEIEHAL 292
>BP068551
Length = 442
Score = 92.8 bits (229), Expect = 1e-19
Identities = 41/78 (52%), Positives = 58/78 (73%)
Frame = -1
Query: 368 FALGAGPVPGLLLTEIFPSRIRAKAMAFCMSVHWVINFFVGLLFLRLLEKLGAQLLYSMF 427
F+LGAGPVP LLL EIF SRIRAKA++ + HW+ NF +GL FL +++K G +Y F
Sbjct: 442 FSLGAGPVPALLLPEIFASRIRAKAISLSLGTHWISNFVIGLYFLSVVDKFGISTVYLGF 263
Query: 428 ATFCIMAVIFVKRNVVET 445
+T C++AV+++ NVVE+
Sbjct: 262 STVCLLAVLYIAGNVVES 209
>TC10790 homologue to UP|Q8GTR0 (Q8GTR0) Sugar transporter, partial (8%)
Length = 647
Score = 79.7 bits (195), Expect = 9e-16
Identities = 40/40 (100%), Positives = 40/40 (100%)
Frame = +1
Query: 422 LLYSMFATFCIMAVIFVKRNVVETKGKSLQEIEIALLPQE 461
LLYSMFATFCIMAVIFVKRNVVETKGKSLQEIEIALLPQE
Sbjct: 1 LLYSMFATFCIMAVIFVKRNVVETKGKSLQEIEIALLPQE 120
>TC9159 similar to UP|HXT4_YEAST (P32467) Low-affinity glucose transporter
HXT4 (Low-affinity glucose transporter LGT1), partial
(5%)
Length = 728
Score = 68.2 bits (165), Expect = 3e-12
Identities = 35/103 (33%), Positives = 55/103 (52%)
Frame = +3
Query: 355 YLSVGGMLLFVFTFALGAGPVPGLLLTEIFPSRIRAKAMAFCMSVHWVINFFVGLLFLRL 414
+L++GG+ L++ F+ G G VP ++ +EI+P R R + WV N V FL L
Sbjct: 114 FLALGGLALYIIFFSPGMGTVPWVVNSEIYPLRYRGICGGIASTTVWVSNLVVSQSFLSL 293
Query: 415 LEKLGAQLLYSMFATFCIMAVIFVKRNVVETKGKSLQEIEIAL 457
+ +G + MF I+ + FV V ETKG ++E+E L
Sbjct: 294 TQTIGTAWTFMMFGIIAIIGIFFVLIFVPETKGVPMEEVESML 422
>TC10135
Length = 1501
Score = 66.2 bits (160), Expect = 1e-11
Identities = 32/95 (33%), Positives = 51/95 (53%)
Frame = +2
Query: 360 GMLLFVFTFALGAGPVPGLLLTEIFPSRIRAKAMAFCMSVHWVINFFVGLLFLRLLEKLG 419
G+ L++ TF+ G G VP ++ +EI+P R R + W+ N V FL L + +G
Sbjct: 902 GLALYIITFSPGMGTVPWVINSEIYPFRYRGVCGGIASTTVWISNLIVSQSFLSLTQTIG 1081
Query: 420 AQLLYSMFATFCIMAVIFVKRNVVETKGKSLQEIE 454
+ MF ++A+ FV V ETKG ++E+E
Sbjct: 1082TAWTFMMFGILAVVAIFFVIVFVPETKGVPMEEVE 1186
>TC9536 similar to UP|Q8LPQ8 (Q8LPQ8) AT4g35300/F23E12_140, partial (17%)
Length = 537
Score = 63.9 bits (154), Expect = 5e-11
Identities = 33/101 (32%), Positives = 56/101 (54%)
Frame = +2
Query: 353 ALYLSVGGMLLFVFTFALGAGPVPGLLLTEIFPSRIRAKAMAFCMSVHWVINFFVGLLFL 412
A+ +V ++ F F F + GP+P +L +EIFP+R+R +A C V W+ + V
Sbjct: 17 AIISTVCVVVYFCF-FVMAYGPIPNILCSEIFPTRVRGLCIAICALVFWIGDIIVTYTLP 193
Query: 413 RLLEKLGAQLLYSMFATFCIMAVIFVKRNVVETKGKSLQEI 453
+L +G ++ ++A C ++ +FV V ETKG L+ I
Sbjct: 194 VMLSSMGLAGVFGLYAIVCCISWVFVYLKVPETKGMPLEVI 316
>BG662351
Length = 407
Score = 60.8 bits (146), Expect = 4e-10
Identities = 35/104 (33%), Positives = 62/104 (58%), Gaps = 2/104 (1%)
Frame = +1
Query: 356 LSVGGMLLFVFTFALGAGPVPGLLLTEIFPSRIRAKAMAFCMSVHWVINFFVGLLFLRLL 415
L+V G+L++V +FA+G G VP ++++EIFP I+ +A + V+W + F L+
Sbjct: 85 LAVTGILVYVGSFAIGMGAVPWVVMSEIFPVNIKGQAGSLATLVNWFGAWLCSYTFNFLM 264
Query: 416 --EKLGAQLLYSMFATFCIMAVIFVKRNVVETKGKSLQEIEIAL 457
G +LY+ I++++ V V ETKGKSL++++ A+
Sbjct: 265 SWSTYGTFILYAAINALGILSIVVV---VPETKGKSLEQLQAAI 387
>TC10748 similar to UP|Q7XA50 (Q7XA50) Sorbitol-like transporter, partial
(21%)
Length = 550
Score = 57.0 bits (136), Expect = 6e-09
Identities = 31/94 (32%), Positives = 55/94 (57%)
Frame = +2
Query: 361 MLLFVFTFALGAGPVPGLLLTEIFPSRIRAKAMAFCMSVHWVINFFVGLLFLRLLEKLGA 420
+L +V TF++GAGP+ + +EIFP R+RA+ A + V+ V + + + FL L + +
Sbjct: 5 VLSYVATFSIGAGPITWVYSSEIFPLRLRAQGCAMGVVVNRVTSGVISMTFLSLSKGITI 184
Query: 421 QLLYSMFATFCIMAVIFVKRNVVETKGKSLQEIE 454
+ +F I IF + ET+GK+L+++E
Sbjct: 185 GGAFFLFGGIAICGWIFFYTMLPETRGKTLEDME 286
>TC16378 similar to GB|AAM70554.1|21700861|AY124845 At1g75220/F22H5_6
{Arabidopsis thaliana;}, partial (31%)
Length = 738
Score = 55.5 bits (132), Expect = 2e-08
Identities = 29/99 (29%), Positives = 56/99 (56%)
Frame = +2
Query: 356 LSVGGMLLFVFTFALGAGPVPGLLLTEIFPSRIRAKAMAFCMSVHWVINFFVGLLFLRLL 415
+SV G+++ V F+LG GP+P ++++EI P I+ A + +W+ ++ + + LL
Sbjct: 152 VSVVGLVVMVIGFSLGLGPIPWVIMSEILPVSIKGLAGSTATMANWLTSWAI-TMTANLL 328
Query: 416 EKLGAQLLYSMFATFCIMAVIFVKRNVVETKGKSLQEIE 454
+ ++M+ V+F V ETKG++L+EI+
Sbjct: 329 LTWSSGGTFTMYTVVAAFTVVFTALWVPETKGRTLEEIQ 445
>TC8113 weakly similar to UP|O23213 (O23213) Sugar transporter like
protein, partial (93%)
Length = 1865
Score = 50.8 bits (120), Expect = 5e-07
Identities = 35/97 (36%), Positives = 57/97 (58%), Gaps = 2/97 (2%)
Frame = +2
Query: 15 DKETTNPSWKLSLPHVLVATITSFLFGYHLGVVNEPLESISVDLGFNGNTLAEGL--VVS 72
DK+TT + L+ V+VA++ S + GY GV++ L I D+G + +T E L +++
Sbjct: 89 DKDTTINKYALAC--VIVASMVSIISGYDTGVMSGALLFIKEDIGIS-DTQQEVLAGILN 259
Query: 73 ICLGGALFGCLLSGWIADAVGRRRAFQLCALPMIIGA 109
IC AL GCL +G +D +GRR L ++ ++GA
Sbjct: 260 IC---ALVGCLAAGKTSDYIGRRYTIFLASILFLVGA 361
>TC12545 similar to UP|Q7XA50 (Q7XA50) Sorbitol-like transporter, partial
(18%)
Length = 482
Score = 48.1 bits (113), Expect = 3e-06
Identities = 27/84 (32%), Positives = 47/84 (55%)
Frame = +2
Query: 371 GAGPVPGLLLTEIFPSRIRAKAMAFCMSVHWVINFFVGLLFLRLLEKLGAQLLYSMFATF 430
G+GP+ + +EIFP R+RA+ +A V+ + + + FL L + + +FA
Sbjct: 2 GSGPITWVYSSEIFPLRLRAQGVAIGAVVNRLTRGVISMTFLSLSNAITIGGAFFLFAGI 181
Query: 431 CIMAVIFVKRNVVETKGKSLQEIE 454
+ A IF + ET+G++L+EIE
Sbjct: 182 AVCAWIFHYTLLPETRGQTLEEIE 253
>TC12303 similar to GB|CAB10424.1|2245004|ATFCA6 membrane transporter like
protein {Arabidopsis thaliana;}, partial (25%)
Length = 545
Score = 48.1 bits (113), Expect = 3e-06
Identities = 34/101 (33%), Positives = 49/101 (47%), Gaps = 5/101 (4%)
Frame = +3
Query: 16 KETTNPSWKLSLPHVL----VATITSFLFGYHLGVVNEPLESISVDL-GFNGNTLAEGLV 70
+E + SWK P+VL A I LFGY GV++ L I D + T + +
Sbjct: 150 RECLSLSWKN--PYVLRRAFSAGIGGLLFGYDTGVISGALLYIKDDFKAVDRKTWLQEAI 323
Query: 71 VSICLGGALFGCLLSGWIADAVGRRRAFQLCALPMIIGAAM 111
VS + GA+ G + GWI D GRR A + +IG+ +
Sbjct: 324 VSTAIAGAIIGAAVGGWINDRFGRRVAIIIADTLFLIGSVI 446
>BP053600
Length = 400
Score = 47.0 bits (110), Expect = 7e-06
Identities = 26/59 (44%), Positives = 37/59 (62%)
Frame = -2
Query: 400 HWVINFFVGLLFLRLLEKLGAQLLYSMFATFCIMAVIFVKRNVVETKGKSLQEIEIALL 458
H+ N V L F L E LGA+ L+ +F ++A++FV +V ETKG SL+EIE +L
Sbjct: 393 HFASNAVVTLAFSPLKELLGAENLFLLFGALSLVALLFVIFSVPETKGLSLEEIESQIL 217
>BP074036
Length = 405
Score = 46.2 bits (108), Expect = 1e-05
Identities = 26/87 (29%), Positives = 50/87 (56%)
Frame = -2
Query: 369 ALGAGPVPGLLLTEIFPSRIRAKAMAFCMSVHWVINFFVGLLFLRLLEKLGAQLLYSMFA 428
++G GPV + +EIFP +RA+ +A C++V+ ++N + F+ + + + A
Sbjct: 398 SIGIGPVTWIYSSEIFPITLRAQGLAVCVAVNRIVNMAMLTSFISIYKAITMGGCLFALA 219
Query: 429 TFCIMAVIFVKRNVVETKGKSLQEIEI 455
++A F + ETKG+SL+++EI
Sbjct: 218 GVNVLAFWFY-FTLPETKGRSLEDMEI 141
>BI420004
Length = 547
Score = 45.4 bits (106), Expect = 2e-05
Identities = 28/75 (37%), Positives = 40/75 (53%), Gaps = 5/75 (6%)
Frame = +1
Query: 28 PHVLVAT----ITSFLFGYHLGVVNEPLESISVDLG-FNGNTLAEGLVVSICLGGALFGC 82
P++L T I LFGY GV++ L I D ++L + +VS+ L GA+ G
Sbjct: 163 PYILGVTAAAGIGGLLFGYDTGVISGALLYIKDDFEEVRRSSLLQETIVSMALVGAIIGA 342
Query: 83 LLSGWIADAVGRRRA 97
GWI DA GR++A
Sbjct: 343 ATGGWINDAFGRKKA 387
>TC19595
Length = 500
Score = 44.7 bits (104), Expect = 3e-05
Identities = 22/82 (26%), Positives = 42/82 (50%)
Frame = +1
Query: 374 PVPGLLLTEIFPSRIRAKAMAFCMSVHWVINFFVGLLFLRLLEKLGAQLLYSMFATFCIM 433
PVP + +EI+P R +V+WV + + FL + + +G +++ ++
Sbjct: 1 PVPWTVNSEIYPEEFRGVCGGMSATVNWVCSVIMSQSFLSISDAVGLGGSFAILGVIAVV 180
Query: 434 AVIFVKRNVVETKGKSLQEIEI 455
A +FV V ETKG + +E+ +
Sbjct: 181 AFLFVLLFVPETKGLTFEEMTL 246
>CB829587
Length = 548
Score = 40.0 bits (92), Expect = 8e-04
Identities = 18/56 (32%), Positives = 34/56 (60%), Gaps = 5/56 (8%)
Frame = +2
Query: 342 TGASTLLPTAGALY-----LSVGGMLLFVFTFALGAGPVPGLLLTEIFPSRIRAKA 392
TG + L G L L++GG+L+++ +++G G VP ++++E+FP I+ A
Sbjct: 374 TGIAFFLKDQGLLLEWVPTLAIGGVLIYIAAYSIGLGSVPWVIMSEVFPIHIKGTA 541
>BI418774
Length = 554
Score = 38.9 bits (89), Expect = 0.002
Identities = 19/58 (32%), Positives = 34/58 (57%)
Frame = +3
Query: 357 SVGGMLLFVFTFALGAGPVPGLLLTEIFPSRIRAKAMAFCMSVHWVINFFVGLLFLRL 414
++ + +FV A+G GPV + +EIFP R+RA+ + C+ V+ + N V F+ +
Sbjct: 381 TIVAIYIFVAFVAIGIGPVTWVYSSEIFPLRLRAQGLGVCVLVNRLANVAVLTSFISI 554
>TC11822 similar to UP|P93051 (P93051) ORFc protein (Fragment), partial
(74%)
Length = 419
Score = 37.4 bits (85), Expect = 0.005
Identities = 27/78 (34%), Positives = 41/78 (51%), Gaps = 1/78 (1%)
Frame = +3
Query: 32 VATITSFLFGYHLGVVNEPLESISVDLGFNGNTLAE-GLVVSICLGGALFGCLLSGWIAD 90
VA S+ FG +G + ++I DL + LAE L SI GA+ G + SG IAD
Sbjct: 165 VAVCGSYEFGAGIGYSSPTQDAIRKDLNLS---LAEYSLFGSIVTFGAMIGAITSGLIAD 335
Query: 91 AVGRRRAFQLCALPMIIG 108
+GR+ A ++ + + G
Sbjct: 336 FIGRKGAMRVSSAFCVAG 389
>TC16504 weakly similar to UP|Q84KI7 (Q84KI7) Sorbitol transporter, partial
(37%)
Length = 903
Score = 37.0 bits (84), Expect = 0.007
Identities = 25/82 (30%), Positives = 41/82 (49%)
Frame = +2
Query: 30 VLVATITSFLFGYHLGVVNEPLESISVDLGFNGNTLAEGLVVSICLGGALFGCLLSGWIA 89
V+ A++ L+GY + V+ L I DL + L V + AL C+ +G IA
Sbjct: 98 VVAASVIFALYGYVMAVMTGVLLFIKEDLQLSD--LQVQFSVGLLHMSALPACMTAGRIA 271
Query: 90 DAVGRRRAFQLCALPMIIGAAM 111
D +GRR L A+ ++G+ +
Sbjct: 272 DYIGRRYTIILTAIIFLVGSVL 337
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.341 0.151 0.500
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,635,824
Number of Sequences: 28460
Number of extensions: 158953
Number of successful extensions: 1446
Number of sequences better than 10.0: 56
Number of HSP's better than 10.0 without gapping: 1425
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1439
length of query: 461
length of database: 4,897,600
effective HSP length: 93
effective length of query: 368
effective length of database: 2,250,820
effective search space: 828301760
effective search space used: 828301760
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.9 bits)
S2: 57 (26.6 bits)
Medicago: description of AC122722.6