BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC122169.1 + phase: 0 /pseudo
(459 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV409748 50 8e-07
TC17095 similar to UP|Q9FMC0 (Q9FMC0) Amino acid transporter-lik... 45 3e-05
AW720246 39 0.002
TC16422 similar to PIR|T05653|T05653 amino acid transport protei... 37 0.007
AV779312 34 0.058
TC13318 similar to UP|CAA20314 (CAA20314) SPBC30B4.01c protein (... 34 0.058
BP064494 33 0.099
CB826779 32 0.29
TC17157 similar to UP|CAD27462 (CAD27462) Nucleosome assembly pr... 30 0.64
TC8606 similar to UP|AAQ82688 (AAQ82688) Epa4p, partial (7%) 30 0.64
AU089408 30 0.83
BP048490 30 1.1
TC11422 UP|NIA_LOTJA (P39869) Nitrate reductase [NADH] (NR) , c... 30 1.1
AV781102 29 1.4
AW164001 29 1.4
AV773558 29 1.4
TC13291 29 1.4
TC10227 UP|Q7Q2V3 (Q7Q2V3) EbiP1409 (Fragment), partial (10%) 29 1.9
TC8557 similar to UP|Q9LK52 (Q9LK52) Dbj|BAA90629.1, complete 29 1.9
TC8259 similar to UP|HMGL_SOYBN (P26585) HMG1/2-like protein (SB... 29 1.9
>AV409748
Length = 417
Score = 50.1 bits (118), Expect = 8e-07
Identities = 30/108 (27%), Positives = 52/108 (47%)
Frame = +2
Query: 139 GYGCTFTQTIFNGLNVLAGVGLLSAPDTVKQAGWASLLVIVVFAVVCFYTAELMRHCFQS 198
G +FT ++FN + G G+++ P +K G A+ + ++F T+ + F
Sbjct: 86 GAAASFTGSVFNLSTTIIGAGIMALPAAIKVLGVAAGIAAIIFLAFLTETSLDILMRFSR 265
Query: 199 REGIISYPDIGEAAFGKYGRVFISIVLYTELYSYCVEFIIMEGDNLSG 246
SY D+ AFG GR+ + I + + V ++I+ GD LSG
Sbjct: 266 VGNARSYGDVMGYAFGWVGRLVLQISVLINNFGILVVYVIIIGDVLSG 409
>TC17095 similar to UP|Q9FMC0 (Q9FMC0) Amino acid transporter-like protein,
partial (17%)
Length = 751
Score = 45.1 bits (105), Expect = 3e-05
Identities = 33/101 (32%), Positives = 53/101 (51%), Gaps = 6/101 (5%)
Frame = +2
Query: 124 EKASI-QKNIPEELPIGY----GCTFTQTIFNGLNVLAGVGLLSAPDTVKQAGWA-SLLV 177
EKA + K+ EE G+ G +F+ +FN + G G+++ P T+KQ G LL
Sbjct: 443 EKAPLLPKSHVEESDAGFDDFNGASFSGAVFNLSTTIIGAGIMALPATLKQLGMIPGLLA 622
Query: 178 IVVFAVVCFYTAELMRHCFQSREGIISYPDIGEAAFGKYGR 218
IV+ A++ + EL+ F +SY + +FGKYG+
Sbjct: 623 IVLMALLTEKSIELLLR-FTRAGKAVSYAGLMGDSFGKYGK 742
>AW720246
Length = 445
Score = 38.9 bits (89), Expect = 0.002
Identities = 25/109 (22%), Positives = 48/109 (43%), Gaps = 6/109 (5%)
Frame = +3
Query: 152 LNVLAGVGLLSAPDTVKQAGW-ASLLVIVVFAVVCFYTAELMRHCFQSREGII-----SY 205
+ + G G+LS ++ Q GW A V++ F+VV YT+ ++ C++ + ++
Sbjct: 96 ITAVVGSGVLSLAWSIAQMGWIAGPGVMIFFSVVALYTSSILAECYRCGDSHFGKRNYTF 275
Query: 206 PDIGEAAFGKYGRVFISIVLYTELYSYCVEFIIMEGDNLSGLFPGTSLH 254
D + G + IV Y L+ + + I +L + LH
Sbjct: 276 MDAVQNILGGFSVTICGIVQYLNLFGSAIGYTIAASMSLIEIRKSFCLH 422
>TC16422 similar to PIR|T05653|T05653 amino acid transport protein homolog
F22I13.20 - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (38%)
Length = 685
Score = 37.0 bits (84), Expect = 0.007
Identities = 27/111 (24%), Positives = 46/111 (41%), Gaps = 9/111 (8%)
Frame = +2
Query: 146 QTIFNGLNVLAGVGLLSAPDTVKQAGWASLLVIVVFAVVCFYTAELMRHCFQSREGIIS- 204
+T N + G G+L P K+ GW + L+++ V F T M +R + S
Sbjct: 194 KTFANIFISIVGAGVLGLPYCFKRTGWLTGLIMLF--TVAFLTYHCMMLLVSTRRKLDSL 367
Query: 205 --------YPDIGEAAFGKYGRVFISIVLYTELYSYCVEFIIMEGDNLSGL 247
+ D+G A G GR+ + ++ +CV ++I L L
Sbjct: 368 TGYSKLGSFGDLGFAICGPLGRLAVDAMIVLSQAGFCVSYLIFIATTLGFL 520
>AV779312
Length = 345
Score = 33.9 bits (76), Expect = 0.058
Identities = 16/52 (30%), Positives = 29/52 (55%), Gaps = 1/52 (1%)
Frame = +3
Query: 152 LNVLAGVGLLSAPDTVKQAGWA-SLLVIVVFAVVCFYTAELMRHCFQSREGI 202
+ + G G+LS + Q GW + I++FA V YT+ L+ C++S + +
Sbjct: 132 ITAVIGAGVLSLAWAMAQLGWILGVSCILIFAGVTLYTSNLLADCYRSPDPV 287
>TC13318 similar to UP|CAA20314 (CAA20314) SPBC30B4.01c protein (SPBC3D6.14C
protein) (Fragment), partial (15%)
Length = 543
Score = 33.9 bits (76), Expect = 0.058
Identities = 15/39 (38%), Positives = 24/39 (61%)
Frame = +3
Query: 9 NKEKENDYFLDANEDETDIEAVNYDSDSSNNNDDDDEDD 47
N+E+E+D + +EDE D + + D D DDDD+D+
Sbjct: 249 NEEEEDDDEEEDDEDEDDDDDDDDDDDGEEEEDDDDDDE 365
Score = 30.8 bits (68), Expect = 0.49
Identities = 13/44 (29%), Positives = 27/44 (60%)
Frame = +3
Query: 4 NNSDKNKEKENDYFLDANEDETDIEAVNYDSDSSNNNDDDDEDD 47
N +++ ++E D D +ED+ D + + D + ++DDDDE++
Sbjct: 249 NEEEEDDDEEED---DEDEDDDDDDDDDDDGEEEEDDDDDDEEE 371
Score = 26.6 bits (57), Expect = 9.2
Identities = 10/28 (35%), Positives = 18/28 (63%)
Frame = +3
Query: 19 DANEDETDIEAVNYDSDSSNNNDDDDED 46
D E+E D E + + + +++DDDD+D
Sbjct: 246 DNEEEEDDDEEEDDEDEDDDDDDDDDDD 329
>BP064494
Length = 354
Score = 33.1 bits (74), Expect = 0.099
Identities = 19/70 (27%), Positives = 31/70 (44%), Gaps = 6/70 (8%)
Frame = +3
Query: 330 AFGIYGFCFAGHSVFPNIYQSM------ANKKDFTKAMLICFVLPVFLYGSVGAAGFLMF 383
A G F +AGH+V I +M +KK K +++ ++ F Y V G+ +F
Sbjct: 141 AMGEVAFSYAGHNVVLEIQATMPSTPEKPSKKPMWKGVVVAYIGVAFCYLPVAFIGYYVF 320
Query: 384 GERTSSQITL 393
G I +
Sbjct: 321 GNSVDDNILI 350
>CB826779
Length = 469
Score = 31.6 bits (70), Expect = 0.29
Identities = 15/47 (31%), Positives = 26/47 (54%)
Frame = +1
Query: 1 MASNNSDKNKEKENDYFLDANEDETDIEAVNYDSDSSNNNDDDDEDD 47
+ S DK K + +Y +D +E V+ ++D ++D+DDEDD
Sbjct: 139 LPSATDDKKKPQPQEY-----DDAPVVEDVHDEADEDEDDDEDDEDD 264
>TC17157 similar to UP|CAD27462 (CAD27462) Nucleosome assembly protein
1-like protein 3, partial (81%)
Length = 1298
Score = 30.4 bits (67), Expect = 0.64
Identities = 17/45 (37%), Positives = 24/45 (52%)
Frame = +2
Query: 3 SNNSDKNKEKENDYFLDANEDETDIEAVNYDSDSSNNNDDDDEDD 47
+ SD +E+D D +EDE D D + ++DDDDEDD
Sbjct: 746 AEQSDFEDIEEDDEDGDEDEDEDD-----DDDEEEEDDDDDDEDD 865
Score = 28.5 bits (62), Expect = 2.4
Identities = 12/37 (32%), Positives = 19/37 (50%)
Frame = +2
Query: 11 EKENDYFLDANEDETDIEAVNYDSDSSNNNDDDDEDD 47
E E F D ED+ D + + D + ++DD+DD
Sbjct: 743 EAEQSDFEDIEEDDEDGDEDEDEDDDDDEEEEDDDDD 853
>TC8606 similar to UP|AAQ82688 (AAQ82688) Epa4p, partial (7%)
Length = 1008
Score = 30.4 bits (67), Expect = 0.64
Identities = 14/46 (30%), Positives = 23/46 (49%)
Frame = -2
Query: 2 ASNNSDKNKEKENDYFLDANEDETDIEAVNYDSDSSNNNDDDDEDD 47
++N S+ K E A +E + + D + ++DDDDEDD
Sbjct: 506 SNNKSNSKKAPEGGPGAGAGPEENGEDEEDEDGEDQEDDDDDDEDD 369
Score = 29.6 bits (65), Expect = 1.1
Identities = 11/26 (42%), Positives = 17/26 (65%)
Frame = -2
Query: 22 EDETDIEAVNYDSDSSNNNDDDDEDD 47
EDE D + + + D ++ DDD+EDD
Sbjct: 431 EDEEDEDGEDQEDDDDDDEDDDEEDD 354
Score = 26.6 bits (57), Expect = 9.2
Identities = 18/71 (25%), Positives = 28/71 (39%)
Frame = -2
Query: 2 ASNNSDKNKEKENDYFLDANEDETDIEAVNYDSDSSNNNDDDDEDDRIATHRPESFTSQQ 61
A ++N E E D + ED+ D D + + DD ED+ E ++
Sbjct: 458 AGAGPEENGEDEEDEDGEDQEDDDD------DDEDDDEEDDGGEDEEEEGVEEEDNEDEE 297
Query: 62 WPQSYNEALDP 72
+ EAL P
Sbjct: 296 EDEEDEEALQP 264
>AU089408
Length = 522
Score = 30.0 bits (66), Expect = 0.83
Identities = 27/94 (28%), Positives = 41/94 (42%), Gaps = 2/94 (2%)
Frame = -2
Query: 242 DNLSGLFPGTS-LHWGSLNLDGKHLFAILAALIILPTVWLKDLRFVSYLSAGGVVGTALV 300
D L L P S + W +L+ LFAI+ +L +WL + L GG G+
Sbjct: 278 DKLPNLNPSASKIKWPVESLNFYKLFAIVGVFRLLSHIWLLCPSLLFLLFLGGFSGS--- 108
Query: 301 GACVYAVGTRKDVGFHHTAPLVNWSGVP-FAFGI 333
GAC + + +G AP+ P F+F +
Sbjct: 107 GACFFRGQSLFLLGHTLVAPIEATKAQPHFSFSL 6
>BP048490
Length = 452
Score = 29.6 bits (65), Expect = 1.1
Identities = 14/43 (32%), Positives = 27/43 (62%)
Frame = +3
Query: 3 SNNSDKNKEKENDYFLDANEDETDIEAVNYDSDSSNNNDDDDE 45
S +SD + E +D + +D+ D++ +SDSS ++D++DE
Sbjct: 114 SASSDSDDEDSSDSDSEVKKDKMDVD----ESDSSEDSDEEDE 230
>TC11422 UP|NIA_LOTJA (P39869) Nitrate reductase [NADH] (NR) , complete
Length = 2932
Score = 29.6 bits (65), Expect = 1.1
Identities = 11/27 (40%), Positives = 20/27 (73%)
Frame = +1
Query: 21 NEDETDIEAVNYDSDSSNNNDDDDEDD 47
++ + AVN D D+S+++DDD++DD
Sbjct: 82 HDPKPSFPAVNLDLDASSSDDDDEKDD 162
>AV781102
Length = 567
Score = 29.3 bits (64), Expect = 1.4
Identities = 15/30 (50%), Positives = 21/30 (70%), Gaps = 2/30 (6%)
Frame = -3
Query: 31 NYDSDSSNNNDDD--DEDDRIATHRPESFT 58
N SDSSN++DDD D+DD AT + + F+
Sbjct: 367 NAHSDSSNHDDDDGGDDDDGDATSQEDLFS 278
>AW164001
Length = 447
Score = 29.3 bits (64), Expect = 1.4
Identities = 23/77 (29%), Positives = 39/77 (49%), Gaps = 10/77 (12%)
Frame = +1
Query: 9 NKEKENDYFLDANEDETDIEAVNYDSDSSNNNDDDDEDDRIAT----HRPE---SFTSQQ 61
NK+ +++ + + + DE ++D D +++DDDD+DD AT +P+ S Q
Sbjct: 37 NKQNDDEVYKEEDVDE------DFDDDD-DDDDDDDDDDEPATLGFVDKPKDEWSLCRQY 195
Query: 62 WPQSYNEA---LDPLTI 75
+P LDPL I
Sbjct: 196 FPSKAGGVPAWLDPLNI 246
>AV773558
Length = 469
Score = 29.3 bits (64), Expect = 1.4
Identities = 11/32 (34%), Positives = 20/32 (62%)
Frame = -2
Query: 16 YFLDANEDETDIEAVNYDSDSSNNNDDDDEDD 47
+F + +E+ D + Y D +++DDDD+DD
Sbjct: 420 FFNEGDEELDDDFVLVYSEDDDDDDDDDDDDD 325
Score = 28.5 bits (62), Expect = 2.4
Identities = 11/38 (28%), Positives = 23/38 (59%)
Frame = -2
Query: 10 KEKENDYFLDANEDETDIEAVNYDSDSSNNNDDDDEDD 47
++ + F + ++E D + V S+ +++DDDD+DD
Sbjct: 441 EDSPDGLFFNEGDEELDDDFVLVYSEDDDDDDDDDDDD 328
Score = 27.3 bits (59), Expect = 5.4
Identities = 12/42 (28%), Positives = 27/42 (63%)
Frame = -2
Query: 6 SDKNKEKENDYFLDANEDETDIEAVNYDSDSSNNNDDDDEDD 47
++ ++E ++D+ L +ED+ D + + D D + DD+DE++
Sbjct: 414 NEGDEELDDDFVLVYSEDDDDDD--DDDDDDDDYTDDEDEEE 295
>TC13291
Length = 564
Score = 29.3 bits (64), Expect = 1.4
Identities = 13/29 (44%), Positives = 15/29 (50%)
Frame = +3
Query: 124 EKASIQKNIPEELPIGYGCTFTQTIFNGL 152
+K I N E L IGYGCT F+ L
Sbjct: 66 DKTYISGNFKESLSIGYGCTILLNAFSAL 152
>TC10227 UP|Q7Q2V3 (Q7Q2V3) EbiP1409 (Fragment), partial (10%)
Length = 624
Score = 28.9 bits (63), Expect = 1.9
Identities = 12/43 (27%), Positives = 21/43 (47%)
Frame = +3
Query: 194 HCFQSREGIISYPDIGEAAFGKYGRVFISIVLYTELYSYCVEF 236
HC R+ ++ +G + GKY +S+ +Y +Y Y F
Sbjct: 486 HCISGRKD*LACMALGSGSHGKYDVSSLSLSIYIYIYIYIYHF 614
>TC8557 similar to UP|Q9LK52 (Q9LK52) Dbj|BAA90629.1, complete
Length = 1064
Score = 28.9 bits (63), Expect = 1.9
Identities = 17/59 (28%), Positives = 29/59 (48%), Gaps = 14/59 (23%)
Frame = +3
Query: 3 SNNSDKNKEKENDYFLDANE--------------DETDIEAVNYDSDSSNNNDDDDEDD 47
S + D++ K + FL+ + D++D+E SD +++DDDDEDD
Sbjct: 381 SYSDDRDHGKSSHLFLEGTKRDVEDHIVARSVDADDSDVEV---KSDEESDDDDDDEDD 548
>TC8259 similar to UP|HMGL_SOYBN (P26585) HMG1/2-like protein (SB11
protein), partial (92%)
Length = 942
Score = 28.9 bits (63), Expect = 1.9
Identities = 11/38 (28%), Positives = 20/38 (51%)
Frame = +2
Query: 10 KEKENDYFLDANEDETDIEAVNYDSDSSNNNDDDDEDD 47
K+ E D E E + VN + +++D+DD+D+
Sbjct: 518 KQAEGPAAADEEESEKSVSEVNDQDEDDDDSDEDDDDE 631
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.327 0.141 0.449
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,601,316
Number of Sequences: 28460
Number of extensions: 123918
Number of successful extensions: 1188
Number of sequences better than 10.0: 59
Number of HSP's better than 10.0 without gapping: 945
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1050
length of query: 459
length of database: 4,897,600
effective HSP length: 93
effective length of query: 366
effective length of database: 2,250,820
effective search space: 823800120
effective search space used: 823800120
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 57 (26.6 bits)
Medicago: description of AC122169.1