BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC122166.9 + phase: 0 /pseudo
(249 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC15418 weakly similar to UP|Q8GT65 (Q8GT65) Serpin-like protein... 201 1e-52
TC15717 weakly similar to UP|Q8GT65 (Q8GT65) Serpin-like protein... 187 1e-48
TC12352 weakly similar to UP|Q8GT65 (Q8GT65) Serpin-like protein... 167 1e-42
AU089397 113 3e-26
TC10904 weakly similar to UP|Q62258 (Q62258) Spi2 proteinase inh... 102 8e-23
AV767474 98 1e-21
BI420912 92 8e-20
AU088851 52 7e-14
BP038032 57 3e-09
BP066474 27 2.5
TC12818 weakly similar to UP|Q8RW91 (Q8RW91) At1g79730/F19K16_29... 27 2.5
BP074788 27 4.3
AV428183 26 5.6
BP039352 26 7.4
TC8154 26 7.4
TC16403 homologue to UP|Q9LKY4 (Q9LKY4) Pti1 kinase-like protein... 25 9.6
>TC15418 weakly similar to UP|Q8GT65 (Q8GT65) Serpin-like protein
(Fragment), partial (44%)
Length = 602
Score = 201 bits (511), Expect = 1e-52
Identities = 110/195 (56%), Positives = 136/195 (69%), Gaps = 11/195 (5%)
Frame = +1
Query: 16 KSLTKFTNVSLNITKHLLSNQKLNEKNIVFSPLSLNTVLIMIATGSEGPTQNQLLSFLQS 75
+S+ T+VSL I K L S +KN VFSPLSL+ VL ++A GSEGPT +QLL+FL+S
Sbjct: 19 ESIETQTDVSLTIAKLLFSKHS-KDKNAVFSPLSLHVVLSILAAGSEGPTLHQLLTFLRS 195
Query: 76 ESTGDLKSLCSQVVSSVLSDGARAGGPCLSYVNGVWVEKSLPLQPSFKQLMTTDFEATLS 135
ST L S SQ+VS VLSD + GGP L + +GVWVE+SL L PSFKQL+T D+ A L+
Sbjct: 196 NSTDHLNSFASQLVSVVLSDASSVGGPRLCFADGVWVEQSLTLNPSFKQLVTADYHAALA 375
Query: 136 AVDFVNK-----------AEKKTKGLIQDLLPHGSVNSLTSLIFANALYFKGVWKQKFDT 184
+VDF K AEK+T GL++DLLP GSV++ T LIFANALYFKG W +KFD
Sbjct: 376 SVDFQTKAVEVANEVNAWAEKETNGLVKDLLPAGSVDASTRLIFANALYFKGAWTEKFDA 555
Query: 185 SKTKDYDFDLLNGKS 199
S TKD DF LL+G S
Sbjct: 556 SMTKDCDFHLLDGTS 600
>TC15717 weakly similar to UP|Q8GT65 (Q8GT65) Serpin-like protein
(Fragment), partial (26%)
Length = 925
Score = 187 bits (476), Expect = 1e-48
Identities = 112/242 (46%), Positives = 151/242 (62%), Gaps = 15/242 (6%)
Frame = +1
Query: 16 KSLTKFT-NVSLNITKHLLSNQKLNEKNIVFSPLSLNTVLIMIATGSEGPTQNQLLSFLQ 74
KS++K +V+L+ L S + + +NIVFSPLSL+ L ++A G+ G T ++LLSFL+
Sbjct: 31 KSISKSQEDVALSFANRLFSTEAYHNENIVFSPLSLHVALAIMAAGAHGSTLDELLSFLR 210
Query: 75 SESTGDLKSLCSQVVSSVLSDGARAGGPC---LSYVNGVWVEKSLPLQPSFKQLMTTDFE 131
+S L ++ SQVVS+V SD A P LS+ NG+WV+KSL L SFKQL+ T ++
Sbjct: 211 FDSVDHLNTIFSQVVSAVFSDNDDAAPPPTHRLSFANGMWVDKSLSLTHSFKQLVATHYK 390
Query: 132 ATLSAVDFVNKA-----------EKKTKGLIQDLLPHGSVNSLTSLIFANALYFKGVWKQ 180
ATL +VDF NKA EK T GLI++LL G+V+ T LIFANAL+FKG W+
Sbjct: 391 ATLDSVDFWNKADQVCDEVNLWVEKGTNGLIKELLSPGAVDKTTRLIFANALHFKGEWEH 570
Query: 181 KFDTSKTKDYDFDLLNGKSVKVPFMTSKNDQFISSLDGFKVLGLPINKAKMGVHSLFTFF 240
KF + Y F LL+G SV VP MT+ +Q I DGFK+LGLP K LF+ +
Sbjct: 571 KFLARYSYSYRFHLLDGTSVVVPLMTNDEEQLIRVFDGFKILGLPY-KQGTDEKRLFSMY 747
Query: 241 FL 242
L
Sbjct: 748 IL 753
>TC12352 weakly similar to UP|Q8GT65 (Q8GT65) Serpin-like protein
(Fragment), partial (25%)
Length = 799
Score = 167 bits (424), Expect = 1e-42
Identities = 96/224 (42%), Positives = 141/224 (62%), Gaps = 12/224 (5%)
Frame = +1
Query: 19 TKFTNVSLNITKHLLSNQKLNEKNIVFSPLSLNTVLIMIATGSEGPTQNQLLSFLQSEST 78
+K +V L+ T+HL S + EKN+++SPLSL L +IA GSEG T ++LLSFL+ +S
Sbjct: 4 SKSMDVPLSFTQHLFSKEDYQEKNLIYSPLSLYAALSVIAAGSEGRTFDELLSFLRFDSI 183
Query: 79 GDLKSLCSQVVSSVLSDGARAGGPCLSYVNGVWVEKSLPLQPSFKQLMTTDFEATLSAVD 138
+L + SQ +S V D A L + NG++++ ++ L F++L++T + A L+++D
Sbjct: 184 DNLNTFFSQAISPVFFDNDAASP--LQHYNGIFIDTTVSLSYPFRRLLSTHYNANLTSLD 357
Query: 139 FVNKA-----------EKKTKGLIQDLLPHGSVNSLTSLIFANALYFKGVWKQKFDTSKT 187
F + E+ T G I LLP G+V +LT LIFANAL F+G+WK KFD T
Sbjct: 358 FNLRGGKVLHEMNSLIEEDTNGHITQLLPPGTVTNLTRLIFANALCFQGMWKHKFD-GLT 534
Query: 188 KDYDFDLLNGKSVKVPFMTS-KNDQFISSLDGFKVLGLPINKAK 230
F+LLNG SVKVPFMT+ KN Q++ + DGFK+L LP + +
Sbjct: 535 YVSPFNLLNGTSVKVPFMTTCKNTQYVRAFDGFKILRLPYKQGR 666
>AU089397
Length = 306
Score = 113 bits (283), Expect = 3e-26
Identities = 59/97 (60%), Positives = 72/97 (73%)
Frame = +3
Query: 43 IVFSPLSLNTVLIMIATGSEGPTQNQLLSFLQSESTGDLKSLCSQVVSSVLSDGARAGGP 102
IVFSPLSL VL +IA GS+G T ++LLSFL S ST L S SQ++S+VLSD A AGGP
Sbjct: 9 IVFSPLSLQVVLSIIAAGSDGSTLDELLSFLGSNSTDHLNSFASQLISTVLSDAAPAGGP 188
Query: 103 CLSYVNGVWVEKSLPLQPSFKQLMTTDFEATLSAVDF 139
L + VWVEKSLP+ SF Q+M TD+ ATL++VDF
Sbjct: 189 RLCFAXXVWVEKSLPVNHSFXQVMNTDYXATLTSVDF 299
>TC10904 weakly similar to UP|Q62258 (Q62258) Spi2 proteinase inhibitor,
partial (8%)
Length = 576
Score = 102 bits (253), Expect = 8e-23
Identities = 58/129 (44%), Positives = 79/129 (60%), Gaps = 5/129 (3%)
Frame = -1
Query: 16 KSLTKFTNVSLNITKHLLSNQKLNEKNIVFSPLSLNTVLIMIATGSEGPTQNQLLSFLQS 75
KS +K +V+L +TKHL S KNIVFSP SL L ++A GS+ T ++LLSFL+
Sbjct: 441 KSKSKSMDVALGLTKHLFSKADYQGKNIVFSPFSLQAALSVMAAGSKDRTLDELLSFLRF 262
Query: 76 ESTGDLKSLCSQVVSSVLSDGARA-----GGPCLSYVNGVWVEKSLPLQPSFKQLMTTDF 130
+S DL + SQV+ VL A A G L + NG+W + SL L FKQL+ T +
Sbjct: 261 DSIDDLTTFFSQVIFPVLISDAAADADTDGSHHLCFANGIWADDSLSLSHRFKQLVATHY 82
Query: 131 EATLSAVDF 139
+ATL+A+DF
Sbjct: 81 KATLTALDF 55
>AV767474
Length = 380
Score = 98.2 bits (243), Expect = 1e-21
Identities = 51/110 (46%), Positives = 76/110 (68%)
Frame = +3
Query: 22 TNVSLNITKHLLSNQKLNEKNIVFSPLSLNTVLIMIATGSEGPTQNQLLSFLQSESTGDL 81
T+V+L ITKHL S Q+ +KN++FSPLSL+ VL ++A GS G T ++LLSFL+ +S L
Sbjct: 63 TDVALTITKHLFSKQEYQDKNLMFSPLSLHAVLSLLAAGSAGSTLDELLSFLRFDSIDHL 242
Query: 82 KSLCSQVVSSVLSDGARAGGPCLSYVNGVWVEKSLPLQPSFKQLMTTDFE 131
+ SQ++S+ A L++VNG+WV+KS+ L SFKQL+ T ++
Sbjct: 243 NTFFSQLISA-----AAPSSHPLTFVNGMWVDKSVSLSNSFKQLLATHYK 377
>BI420912
Length = 508
Score = 92.0 bits (227), Expect = 8e-20
Identities = 53/132 (40%), Positives = 83/132 (62%)
Frame = +2
Query: 6 RKRTKKNLIGKSLTKFTNVSLNITKHLLSNQKLNEKNIVFSPLSLNTVLIMIATGSEGPT 65
R T + KS + + L+ TKH+LS ++ EKN+VFSPLSL L ++A G++G T
Sbjct: 128 RSTTMAMDLQKSKSNSIDAVLSFTKHVLSKEEYQEKNLVFSPLSLYAALSVMAAGADGLT 307
Query: 66 QNQLLSFLQSESTGDLKSLCSQVVSSVLSDGARAGGPCLSYVNGVWVEKSLPLQPSFKQL 125
++LLSFL+ +S L + SQ +S VL LS+ NG+W ++SL + SFKQL
Sbjct: 308 LDELLSFLRFDSVDHLTTFFSQDLSPVLFSDDH-----LSFANGMWGDQSLSIFHSFKQL 472
Query: 126 MTTDFEATLSAV 137
++T ++ATL+++
Sbjct: 473 VSTHYKATLASL 508
>AU088851
Length = 663
Score = 51.6 bits (122), Expect(2) = 7e-14
Identities = 34/88 (38%), Positives = 53/88 (59%), Gaps = 2/88 (2%)
Frame = +3
Query: 60 GSEGPTQNQLLSFLQSESTGDLKSLCSQVVSSVL-SDGARAGGPCLSYVNGVWVEKSLPL 118
GSEG T ++LLSFL+ +ST L S SQ+VS VL + A P LS+ + +W +K+L L
Sbjct: 267 GSEGSTLDELLSFLRFDSTDRLNSFTSQLVSDVLYVNDAAPPSPRLSFASRMWADKTLFL 446
Query: 119 Q-PSFKQLMTTDFEATLSAVDFVNKAEK 145
PS+ + + L+++DF + +K
Sbjct: 447 SLPSYNLWLL--IKELLASLDFXKQGKK 524
Score = 40.8 bits (94), Expect(2) = 7e-14
Identities = 19/43 (44%), Positives = 31/43 (71%)
Frame = +1
Query: 16 KSLTKFTNVSLNITKHLLSNQKLNEKNIVFSPLSLNTVLIMIA 58
+S + ++V+L +TKHL S + +N+VFSPLSL+ VL ++A
Sbjct: 133 ESTSHQSDVALTLTKHLFSKEVYQHENLVFSPLSLHVVLSIMA 261
>BP038032
Length = 437
Score = 57.0 bits (136), Expect = 3e-09
Identities = 43/147 (29%), Positives = 76/147 (51%), Gaps = 9/147 (6%)
Frame = +1
Query: 8 RTKKNLIGKSLTKFTNVSLNITKHLLSNQKLNEKNIVFSPLSLNTVLIMIATGSEGPTQN 67
RT + KS+ T+V+L++TKHL S ++ +KN++FSPLSL+ L ++A GS G T +
Sbjct: 4 RTATMDVEKSMRCQTDVALSVTKHLFSKEEYQQKNLIFSPLSLHVALSVMAGGSAGGTLS 183
Query: 68 QLLSFLQSESTGDLKSLCSQVVSSVLSDGARAGGPC------LSYVNGVWVE---KSLPL 118
+ +FL G + + + +L+ P L NG+WV+ P+
Sbjct: 184 R--TFLSFFGIGSHWKNFNNLSNLILNFHRGPWTPVVISPLRLPLANGMWVD*ITFHFPI 357
Query: 119 QPSFKQLMTTDFEATLSAVDFVNKAEK 145
P+ ++ATL+++DF ++
Sbjct: 358 LPN--NW*PLHYKATLASLDFQKNGDQ 432
>BP066474
Length = 276
Score = 27.3 bits (59), Expect = 2.5
Identities = 15/43 (34%), Positives = 21/43 (47%)
Frame = +2
Query: 14 IGKSLTKFTNVSLNITKHLLSNQKLNEKNIVFSPLSLNTVLIM 56
+ K L F N+SL K S K+ +FSP S N V ++
Sbjct: 41 VPKLLFLFLNLSLQ*HKTSFSASSQKSKSSIFSPFSXNXVYLL 169
>TC12818 weakly similar to UP|Q8RW91 (Q8RW91) At1g79730/F19K16_29, partial
(11%)
Length = 531
Score = 27.3 bits (59), Expect = 2.5
Identities = 10/15 (66%), Positives = 11/15 (72%)
Frame = -1
Query: 167 IFANALYFKGVWKQK 181
+F N YFKGVWK K
Sbjct: 504 LFLNNFYFKGVWKTK 460
>BP074788
Length = 510
Score = 26.6 bits (57), Expect = 4.3
Identities = 25/74 (33%), Positives = 34/74 (45%)
Frame = +2
Query: 97 ARAGGPCLSYVNGVWVEKSLPLQPSFKQLMTTDFEATLSAVDFVNKAEKKTKGLIQDLLP 156
AR G L+Y V+VEK L +QP+ K+L L A D AE TK L +++
Sbjct: 182 ARVHGTKLAYFY*VFVEKVLRVQPNVKKLY-----LLLRASD----AESATKRLHNEIIE 334
Query: 157 HGSVNSLTSLIFAN 170
L + AN
Sbjct: 335 KDLFRLLKENLGAN 376
>AV428183
Length = 244
Score = 26.2 bits (56), Expect = 5.6
Identities = 11/38 (28%), Positives = 22/38 (56%)
Frame = +3
Query: 63 GPTQNQLLSFLQSESTGDLKSLCSQVVSSVLSDGARAG 100
GPT N S ++ G++ S+C++ + V+ D + +G
Sbjct: 75 GPTANPTGSVYSNKEIGEILSICAKFGARVIIDTSSSG 188
>BP039352
Length = 528
Score = 25.8 bits (55), Expect = 7.4
Identities = 15/35 (42%), Positives = 20/35 (56%)
Frame = -1
Query: 213 ISSLDGFKVLGLPINKAKMGVHSLFTFFFLTKKMV 247
+S+L F +LG + MG HSL TFF + K V
Sbjct: 117 LSNLSDFHILGFMM----MGSHSLCTFFSCSIKKV 25
>TC8154
Length = 503
Score = 25.8 bits (55), Expect = 7.4
Identities = 14/35 (40%), Positives = 21/35 (60%)
Frame = -3
Query: 66 QNQLLSFLQSESTGDLKSLCSQVVSSVLSDGARAG 100
QN++ F S ++ +L S++ SSVL GAR G
Sbjct: 324 QNRMSKFATSATSARHYTLFSRLASSVLIHGARHG 220
>TC16403 homologue to UP|Q9LKY4 (Q9LKY4) Pti1 kinase-like protein, partial
(55%)
Length = 876
Score = 25.4 bits (54), Expect = 9.6
Identities = 13/35 (37%), Positives = 20/35 (57%)
Frame = +1
Query: 23 NVSLNITKHLLSNQKLNEKNIVFSPLSLNTVLIMI 57
N+S+ +H+LS LN FS +L +LI+I
Sbjct: 88 NISMRRQRHILSIVTLNLVTYCFSMTTLQRLLILI 192
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.317 0.133 0.375
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,846,921
Number of Sequences: 28460
Number of extensions: 45401
Number of successful extensions: 269
Number of sequences better than 10.0: 32
Number of HSP's better than 10.0 without gapping: 260
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 260
length of query: 249
length of database: 4,897,600
effective HSP length: 88
effective length of query: 161
effective length of database: 2,393,120
effective search space: 385292320
effective search space used: 385292320
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 54 (25.4 bits)
Medicago: description of AC122166.9