BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC122166.8 + phase: 0
(404 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC15717 weakly similar to UP|Q8GT65 (Q8GT65) Serpin-like protein... 283 5e-77
TC12352 weakly similar to UP|Q8GT65 (Q8GT65) Serpin-like protein... 233 6e-62
TC15418 weakly similar to UP|Q8GT65 (Q8GT65) Serpin-like protein... 222 8e-59
AU089397 114 3e-26
TC10904 weakly similar to UP|Q62258 (Q62258) Spi2 proteinase inh... 111 2e-25
AV767474 100 3e-22
AW428704 98 2e-21
BI420912 96 1e-20
TC15011 similar to UP|Q8GT65 (Q8GT65) Serpin-like protein (Fragm... 86 8e-18
BP048545 86 1e-17
TC16721 weakly similar to UP|P93692 (P93692) Serpin, partial (9%) 71 3e-13
TC10025 weakly similar to UP|Q8GT65 (Q8GT65) Serpin-like protein... 70 5e-13
TC11309 similar to UP|Q9FUV8 (Q9FUV8) Phloem serpin-1, partial (... 64 4e-11
TC19574 weakly similar to UP|Q8GT65 (Q8GT65) Serpin-like protein... 62 1e-10
BP034134 62 2e-10
BP039383 62 2e-10
BP038032 60 6e-10
TC9744 similar to UP|Q40066 (Q40066) Protein zx, partial (7%) 60 6e-10
AU088851 57 4e-09
TC12056 weakly similar to UP|ILEU_HUMAN (P30740) Leukocyte elast... 54 6e-08
>TC15717 weakly similar to UP|Q8GT65 (Q8GT65) Serpin-like protein
(Fragment), partial (26%)
Length = 925
Score = 283 bits (723), Expect = 5e-77
Identities = 152/290 (52%), Positives = 197/290 (67%), Gaps = 4/290 (1%)
Frame = +1
Query: 23 NVSLNITKHLLSNQKLNEKNVVFSPLSLNTVLIMITAGSEGPTQNQLLSFLQSESTGDLK 82
+V+L+ L S + + +N+VFSPLSL+ L ++ AG+ G T ++LLSFL+ +S L
Sbjct: 55 DVALSFANRLFSTEAYHNENIVFSPLSLHVALAIMAAGAHGSTLDELLSFLRFDSVDHLN 234
Query: 83 SLCSQLVSSVLSDGAPAGGPC---LSHVNGVWVEQSLPLQPSFKQLMTTDFKATLAAVDF 139
++ SQ+VS+V SD A P LS NG+WV++SL L SFKQL+ T +KATL +VDF
Sbjct: 235 TIFSQVVSAVFSDNDDAAPPPTHRLSFANGMWVDKSLSLTHSFKQLVATHYKATLDSVDF 414
Query: 140 INKADEVIKEVNLWANKETKGFINDLLPRGSVDSLTSLIFANALYFKGVWKRPFDTSKTK 199
NKAD+V EVNLW K T G I +LL G+VD T LIFANAL+FKG W+ F +
Sbjct: 415 WNKADQVCDEVNLWVEKGTNGLIKELLSPGAVDKTTRLIFANALHFKGEWEHKFLARYSY 594
Query: 200 DYDFDLLNGKSVKVPFMTSKNNQFISSFDGFKVLGLPYKQGND-KRAFSIYFFLPDEKDG 258
Y F LL+G SV VP MT+ Q I FDGFK+LGLPYKQG D KR FS+Y LP KDG
Sbjct: 595 SYRFHLLDGTSVVVPLMTNDEEQLIRVFDGFKILGLPYKQGTDEKRLFSMYILLPHAKDG 774
Query: 259 LSALIDKVASDSEFLEQKLPRNQVKVGKFMIPRFNISFEIEASELLNKLG 308
LS LI K+AS+ FLE KLP+ +VK+ F+IPRF+ISF EAS++L + G
Sbjct: 775 LSDLIRKMASEPGFLEGKLPQQKVKLNFFLIPRFDISFAFEASDVLKEFG 924
>TC12352 weakly similar to UP|Q8GT65 (Q8GT65) Serpin-like protein
(Fragment), partial (25%)
Length = 799
Score = 233 bits (593), Expect = 6e-62
Identities = 127/264 (48%), Positives = 180/264 (68%), Gaps = 2/264 (0%)
Frame = +1
Query: 23 NVSLNITKHLLSNQKLNEKNVVFSPLSLNTVLIMITAGSEGPTQNQLLSFLQSESTGDLK 82
+V L+ T+HL S + EKN+++SPLSL L +I AGSEG T ++LLSFL+ +S +L
Sbjct: 16 DVPLSFTQHLFSKEDYQEKNLIYSPLSLYAALSVIAAGSEGRTFDELLSFLRFDSIDNLN 195
Query: 83 SLCSQLVSSVLSDGAPAGGPCLSHVNGVWVEQSLPLQPSFKQLMTTDFKATLAAVDFINK 142
+ SQ +S V D A L H NG++++ ++ L F++L++T + A L ++DF +
Sbjct: 196 TFFSQAISPVFFDNDAASP--LQHYNGIFIDTTVSLSYPFRRLLSTHYNANLTSLDFNLR 369
Query: 143 ADEVIKEVNLWANKETKGFINDLLPRGSVDSLTSLIFANALYFKGVWKRPFDTSKTKDYD 202
+V+ E+N ++T G I LLP G+V +LT LIFANAL F+G+WK FD T
Sbjct: 370 GGKVLHEMNSLIEEDTNGHITQLLPPGTVTNLTRLIFANALCFQGMWKHKFD-GLTYVSP 546
Query: 203 FDLLNGKSVKVPFMTS-KNNQFISSFDGFKVLGLPYKQGNDK-RAFSIYFFLPDEKDGLS 260
F+LLNG SVKVPFMT+ KN Q++ +FDGFK+L LPYKQG D+ R FS+ FLPD +DGLS
Sbjct: 547 FNLLNGTSVKVPFMTTCKNTQYVRAFDGFKILRLPYKQGRDRQRRFSMCIFLPDAQDGLS 726
Query: 261 ALIDKVASDSEFLEQKLPRNQVKV 284
ALI K++S+ FL+ KLPR +V+V
Sbjct: 727 ALIQKLSSEPCFLKGKLPRRKVRV 798
>TC15418 weakly similar to UP|Q8GT65 (Q8GT65) Serpin-like protein
(Fragment), partial (44%)
Length = 602
Score = 222 bits (566), Expect = 8e-59
Identities = 119/195 (61%), Positives = 137/195 (70%)
Frame = +1
Query: 16 KSLTNLTNVSLNITKHLLSNQKLNEKNVVFSPLSLNTVLIMITAGSEGPTQNQLLSFLQS 75
+S+ T+VSL I K L S +KN VFSPLSL+ VL ++ AGSEGPT +QLL+FL+S
Sbjct: 19 ESIETQTDVSLTIAKLLFSKHS-KDKNAVFSPLSLHVVLSILAAGSEGPTLHQLLTFLRS 195
Query: 76 ESTGDLKSLCSQLVSSVLSDGAPAGGPCLSHVNGVWVEQSLPLQPSFKQLMTTDFKATLA 135
ST L S SQLVS VLSD + GGP L +GVWVEQSL L PSFKQL+T D+ A LA
Sbjct: 196 NSTDHLNSFASQLVSVVLSDASSVGGPRLCFADGVWVEQSLTLNPSFKQLVTADYHAALA 375
Query: 136 AVDFINKADEVIKEVNLWANKETKGFINDLLPRGSVDSLTSLIFANALYFKGVWKRPFDT 195
+VDF KA EV EVN WA KET G + DLLP GSVD+ T LIFANALYFKG W FD
Sbjct: 376 SVDFQTKAVEVANEVNAWAEKETNGLVKDLLPAGSVDASTRLIFANALYFKGAWTEKFDA 555
Query: 196 SKTKDYDFDLLNGKS 210
S TKD DF LL+G S
Sbjct: 556 SMTKDCDFHLLDGTS 600
>AU089397
Length = 306
Score = 114 bits (285), Expect = 3e-26
Identities = 59/97 (60%), Positives = 71/97 (72%)
Frame = +3
Query: 43 VVFSPLSLNTVLIMITAGSEGPTQNQLLSFLQSESTGDLKSLCSQLVSSVLSDGAPAGGP 102
+VFSPLSL VL +I AGS+G T ++LLSFL S ST L S SQL+S+VLSD APAGGP
Sbjct: 9 IVFSPLSLQVVLSIIAAGSDGSTLDELLSFLGSNSTDHLNSFASQLISTVLSDAAPAGGP 188
Query: 103 CLSHVNGVWVEQSLPLQPSFKQLMTTDFKATLAAVDF 139
L VWVE+SLP+ SF Q+M TD+ ATL +VDF
Sbjct: 189 RLCFAXXVWVEKSLPVNHSFXQVMNTDYXATLTSVDF 299
>TC10904 weakly similar to UP|Q62258 (Q62258) Spi2 proteinase inhibitor,
partial (8%)
Length = 576
Score = 111 bits (277), Expect = 2e-25
Identities = 65/148 (43%), Positives = 87/148 (57%), Gaps = 5/148 (3%)
Frame = -1
Query: 16 KSLTNLTNVSLNITKHLLSNQKLNEKNVVFSPLSLNTVLIMITAGSEGPTQNQLLSFLQS 75
KS + +V+L +TKHL S KN+VFSP SL L ++ AGS+ T ++LLSFL+
Sbjct: 441 KSKSKSMDVALGLTKHLFSKADYQGKNIVFSPFSLQAALSVMAAGSKDRTLDELLSFLRF 262
Query: 76 ESTGDLKSLCSQLVSSVLSDGAPA-----GGPCLSHVNGVWVEQSLPLQPSFKQLMTTDF 130
+S DL + SQ++ VL A A G L NG+W + SL L FKQL+ T +
Sbjct: 261 DSIDDLTTFFSQVIFPVLISDAAADADTDGSHHLCFANGIWADDSLSLSHRFKQLVATHY 82
Query: 131 KATLAAVDFINKADEVIKEVNLWANKET 158
KATL A+DF + EV +EVN W KET
Sbjct: 81 KATLTALDF--QTTEVHREVNSWIEKET 4
>AV767474
Length = 380
Score = 100 bits (250), Expect = 3e-22
Identities = 54/110 (49%), Positives = 78/110 (70%)
Frame = +3
Query: 22 TNVSLNITKHLLSNQKLNEKNVVFSPLSLNTVLIMITAGSEGPTQNQLLSFLQSESTGDL 81
T+V+L ITKHL S Q+ +KN++FSPLSL+ VL ++ AGS G T ++LLSFL+ +S L
Sbjct: 63 TDVALTITKHLFSKQEYQDKNLMFSPLSLHAVLSLLAAGSAGSTLDELLSFLRFDSIDHL 242
Query: 82 KSLCSQLVSSVLSDGAPAGGPCLSHVNGVWVEQSLPLQPSFKQLMTTDFK 131
+ SQL+S+ AP+ P L+ VNG+WV++S+ L SFKQL+ T +K
Sbjct: 243 NTFFSQLISA----AAPSSHP-LTFVNGMWVDKSVSLSNSFKQLLATHYK 377
>AW428704
Length = 369
Score = 98.2 bits (243), Expect = 2e-21
Identities = 66/139 (47%), Positives = 81/139 (57%), Gaps = 5/139 (3%)
Frame = +1
Query: 222 QFISSFDGFKVLGLPYKQGND-KRAFSIYFFLPDEKDGLSALIDKVASDSEFLEQKLPRN 280
+ I FDGFK+LGLPYKQG D KR FS+Y LP KDGLS LI K+AS+ FL
Sbjct: 13 RLIRVFDGFKILGLPYKQGTDEKRLFSMYILLPHAKDGLSDLIRKMASEPGFL------- 171
Query: 281 QVKVGKFMIPRFNISFEIEASELLNKLGLTLPFSK--GGLTKM--VDSPISQELSVTSIF 336
EAS++L + G+ PFS+ TKM V+SP+ LSV SIF
Sbjct: 172 ------------------EASDVLKEFGVVSPFSQRDADFTKMVKVNSPL-DALSVESIF 294
Query: 337 QKSFIELNEEGTIAAATAR 355
QK FI++NE+GT AAA R
Sbjct: 295 QKVFIKVNEQGTEAAAAPR 351
>BI420912
Length = 508
Score = 95.9 bits (237), Expect = 1e-20
Identities = 59/133 (44%), Positives = 86/133 (64%), Gaps = 1/133 (0%)
Frame = +2
Query: 6 RRTSEQNLIGKSLTNLTNVSLNITKHLLSNQKLNEKNVVFSPLSLNTVLIMITAGSEGPT 65
R T+ + KS +N + L+ TKH+LS ++ EKN+VFSPLSL L ++ AG++G T
Sbjct: 128 RSTTMAMDLQKSKSNSIDAVLSFTKHVLSKEEYQEKNLVFSPLSLYAALSVMAAGADGLT 307
Query: 66 QNQLLSFLQSESTGDLKSLCSQLVSSVL-SDGAPAGGPCLSHVNGVWVEQSLPLQPSFKQ 124
++LLSFL+ +S L + SQ +S VL SD LS NG+W +QSL + SFKQ
Sbjct: 308 LDELLSFLRFDSVDHLTTFFSQDLSPVLFSDDH------LSFANGMWGDQSLSIFHSFKQ 469
Query: 125 LMTTDFKATLAAV 137
L++T +KATLA++
Sbjct: 470 LVSTHYKATLASL 508
>TC15011 similar to UP|Q8GT65 (Q8GT65) Serpin-like protein (Fragment),
partial (20%)
Length = 623
Score = 86.3 bits (212), Expect = 8e-18
Identities = 49/88 (55%), Positives = 63/88 (70%), Gaps = 1/88 (1%)
Frame = +2
Query: 318 LTKMVDSP-ISQELSVTSIFQKSFIELNEEGTIAAATARGSTGGAAPFRLPPPPPIDFVA 376
LTKMVDS + Q L V++IF KSFIE+NEEGT AAA + + + L +DFVA
Sbjct: 17 LTKMVDSSTVGQGLYVSNIFHKSFIEVNEEGTEAAAASAVTIKLRS---LQVLTRVDFVA 187
Query: 377 DHPFLFLIREEFSGTILFVGKVVNPLDG 404
DHPFLF+IRE+ +GT+LF G+V+NPL G
Sbjct: 188 DHPFLFVIREDLTGTVLFTGQVLNPLAG 271
>BP048545
Length = 452
Score = 85.9 bits (211), Expect = 1e-17
Identities = 48/83 (57%), Positives = 59/83 (70%)
Frame = -2
Query: 320 KMVDSPISQELSVTSIFQKSFIELNEEGTIAAATARGSTGGAAPFRLPPPPPIDFVADHP 379
KMVDS +L V++IF KSFIE+NEEGT AAA + G + P P +DFVADHP
Sbjct: 451 KMVDS----DLYVSNIFHKSFIEVNEEGTRAAAASAGIVLKRS--LSPRPTRVDFVADHP 290
Query: 380 FLFLIREEFSGTILFVGKVVNPL 402
F F+IREE +GTILF G+V+NPL
Sbjct: 289 FFFVIREEMTGTILFTGQVLNPL 221
>TC16721 weakly similar to UP|P93692 (P93692) Serpin, partial (9%)
Length = 553
Score = 71.2 bits (173), Expect = 3e-13
Identities = 30/38 (78%), Positives = 36/38 (93%)
Frame = +2
Query: 367 PPPPPIDFVADHPFLFLIREEFSGTILFVGKVVNPLDG 404
PPPP ++F+ADHPFLFLIRE+FSGTILFVG+V+NPL G
Sbjct: 20 PPPPGLEFIADHPFLFLIREDFSGTILFVGQVLNPLGG 133
>TC10025 weakly similar to UP|Q8GT65 (Q8GT65) Serpin-like protein
(Fragment), partial (15%)
Length = 672
Score = 70.5 bits (171), Expect = 5e-13
Identities = 39/65 (60%), Positives = 46/65 (70%), Gaps = 2/65 (3%)
Frame = +1
Query: 342 ELNEEGTIAAAT--ARGSTGGAAPFRLPPPPPIDFVADHPFLFLIREEFSGTILFVGKVV 399
E+NEE T+A A AR GGA P IDFVADHPFL L+RE+ +GTILFVG+V+
Sbjct: 1 EVNEEETVAVAATAARMVLGGAGLC-----PHIDFVADHPFLLLVREDITGTILFVGQVL 165
Query: 400 NPLDG 404
NPLDG
Sbjct: 166 NPLDG 180
>TC11309 similar to UP|Q9FUV8 (Q9FUV8) Phloem serpin-1, partial (10%)
Length = 472
Score = 63.9 bits (154), Expect = 4e-11
Identities = 36/71 (50%), Positives = 48/71 (66%), Gaps = 2/71 (2%)
Frame = +3
Query: 333 TSIFQKSFIELNEEGTIAAATARGSTGGAAPFRLPPP--PPIDFVADHPFLFLIREEFSG 390
T+ F + ++L+EEGT AAA +T R P IDFVADHPF FLIRE+F+G
Sbjct: 81 TAYFTRLSLKLDEEGTTAAA----ATAMLLASRSGPSVRAGIDFVADHPFFFLIREDFTG 248
Query: 391 TILFVGKVVNP 401
TILF+G+V++P
Sbjct: 249 TILFIGQVLHP 281
>TC19574 weakly similar to UP|Q8GT65 (Q8GT65) Serpin-like protein
(Fragment), partial (8%)
Length = 414
Score = 62.4 bits (150), Expect = 1e-10
Identities = 34/54 (62%), Positives = 39/54 (71%)
Frame = -3
Query: 351 AATARGSTGGAAPFRLPPPPPIDFVADHPFLFLIREEFSGTILFVGKVVNPLDG 404
A A GS G FR P IDFVA+HPFLFLIREE +GT LF+G+V+NPLDG
Sbjct: 403 ATAAAGSLLGTY-FR---PAGIDFVANHPFLFLIREELTGTFLFIGQVLNPLDG 254
>BP034134
Length = 556
Score = 62.0 bits (149), Expect = 2e-10
Identities = 27/36 (75%), Positives = 32/36 (88%)
Frame = -1
Query: 369 PPPIDFVADHPFLFLIREEFSGTILFVGKVVNPLDG 404
P IDF ADHPFLFLIRE+ +GTILF+G+V+NPLDG
Sbjct: 490 PTGIDFEADHPFLFLIREDLTGTILFIGQVLNPLDG 383
>BP039383
Length = 340
Score = 61.6 bits (148), Expect = 2e-10
Identities = 29/40 (72%), Positives = 33/40 (82%), Gaps = 3/40 (7%)
Frame = -1
Query: 368 PPPP---IDFVADHPFLFLIREEFSGTILFVGKVVNPLDG 404
PP P +DFVADHPFLF+IREE +GTILF G+VVNPL G
Sbjct: 325 PPSPKTRVDFVADHPFLFVIREEMTGTILFTGQVVNPLAG 206
>BP038032
Length = 437
Score = 60.1 bits (144), Expect = 6e-10
Identities = 44/141 (31%), Positives = 73/141 (51%), Gaps = 9/141 (6%)
Frame = +1
Query: 14 IGKSLTNLTNVSLNITKHLLSNQKLNEKNVVFSPLSLNTVLIMITAGSEGPTQNQLLSFL 73
+ KS+ T+V+L++TKHL S ++ +KN++FSPLSL+ L ++ GS G T ++ +FL
Sbjct: 22 VEKSMRCQTDVALSVTKHLFSKEEYQQKNLIFSPLSLHVALSVMAGGSAGGTLSR--TFL 195
Query: 74 QSESTGDLKSLCSQLVSSVLSDGAPAGGPC------LSHVNGVWVE---QSLPLQPSFKQ 124
G + L + +L+ P L NG+WV+ P+ P+
Sbjct: 196 SFFGIGSHWKNFNNLSNLILNFHRGPWTPVVISPLRLPLANGMWVD*ITFHFPILPN--N 369
Query: 125 LMTTDFKATLAAVDFINKADE 145
+KATLA++DF D+
Sbjct: 370 W*PLHYKATLASLDFQKNGDQ 432
>TC9744 similar to UP|Q40066 (Q40066) Protein zx, partial (7%)
Length = 527
Score = 60.1 bits (144), Expect = 6e-10
Identities = 26/31 (83%), Positives = 31/31 (99%)
Frame = +1
Query: 374 FVADHPFLFLIREEFSGTILFVGKVVNPLDG 404
FVADHPFLFLIRE+F+GTILFVG+V+NPL+G
Sbjct: 73 FVADHPFLFLIREDFTGTILFVGQVLNPLEG 165
>AU088851
Length = 663
Score = 57.4 bits (137), Expect = 4e-09
Identities = 42/108 (38%), Positives = 61/108 (55%), Gaps = 3/108 (2%)
Frame = +3
Query: 59 AGSEGPTQNQLLSFLQSESTGDLKSLCSQLVSSVL--SDGAPAGGPCLSHVNGVWVEQSL 116
AGSEG T ++LLSFL+ +ST L S SQLVS VL +D AP P LS + +W +++L
Sbjct: 264 AGSEGSTLDELLSFLRFDSTDRLNSFTSQLVSDVLYVNDAAPP-SPRLSFASRMWADKTL 440
Query: 117 PLQ-PSFKQLMTTDFKATLAAVDFINKADEVIKEVNLWANKETKGFIN 163
L PS+ + K LA++DF + + + V +W K+ N
Sbjct: 441 FLSLPSYNLWLL--IKELLASLDFXKQGKKXCR-V*IWVGKKXMSIKN 575
Score = 42.4 bits (98), Expect = 1e-04
Identities = 27/69 (39%), Positives = 41/69 (59%), Gaps = 1/69 (1%)
Frame = +1
Query: 8 TSEQNLIGKSLTNLTNVSLNITKHLLSNQKLNEKNVVFSPLSLNTVL-IMITAGSEGPTQ 66
TS + +S ++ ++V+L +TKHL S + +N+VFSPLSL+ VL IM + P
Sbjct: 109 TSRTMDLKESTSHQSDVALTLTKHLFSKEVYQHENLVFSPLSLHVVLSIMAPLVQKAP-- 282
Query: 67 NQLLSFLQS 75
L+SF S
Sbjct: 283 -HLMSFFPS 306
>TC12056 weakly similar to UP|ILEU_HUMAN (P30740) Leukocyte elastase
inhibitor (LEI) (Monocyte/neutrophil elastase inhibitor)
(M/NEI) (EI), partial (6%)
Length = 401
Score = 53.5 bits (127), Expect = 6e-08
Identities = 22/29 (75%), Positives = 29/29 (99%)
Frame = +2
Query: 373 DFVADHPFLFLIREEFSGTILFVGKVVNP 401
+FVADHPFLFLIRE+F+GTILF+G+V++P
Sbjct: 2 NFVADHPFLFLIREDFTGTILFIGQVLHP 88
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.317 0.136 0.385
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,143,476
Number of Sequences: 28460
Number of extensions: 79716
Number of successful extensions: 719
Number of sequences better than 10.0: 53
Number of HSP's better than 10.0 without gapping: 687
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 699
length of query: 404
length of database: 4,897,600
effective HSP length: 92
effective length of query: 312
effective length of database: 2,279,280
effective search space: 711135360
effective search space used: 711135360
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 56 (26.2 bits)
Medicago: description of AC122166.8