BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC122166.12 - phase: 0 /pseudo
(170 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV418280 70 1e-13
AV427022 69 4e-13
AV412895 59 3e-10
AV428268 58 1e-09
BP057427 55 6e-09
TC14968 similar to UP|Q8TTE6 (Q8TTE6) Bacterial extracellular so... 54 1e-08
AU089120 49 5e-07
TC15601 49 5e-07
AU089037 48 1e-06
BP034765 47 1e-06
TC11392 similar to UP|Q8BGI0 (Q8BGI0) Sterol-C5-desaturase, part... 44 2e-05
BP033775 43 2e-05
TC20217 42 4e-05
BP079663 40 3e-04
AV411435 39 4e-04
BP065246 39 4e-04
TC16776 39 4e-04
BP041113 39 5e-04
TC15307 30 0.002
TC19293 37 0.002
>AV418280
Length = 398
Score = 70.5 bits (171), Expect = 1e-13
Identities = 38/122 (31%), Positives = 63/122 (51%), Gaps = 1/122 (0%)
Frame = -2
Query: 48 VLESAQHLFLSCSYFALLWSLVRDWIGF-VGVDTNVLSDHFVQFVHSTGGSKASQSFLQL 106
V E + HLF +C + +W + W+G V + + L++ F QF T +K +
Sbjct: 370 VEECSGHLFFTCVFSMGVWQALHRWLGISVALPASTLAN-FAQF-SITARNKNQRLGELA 197
Query: 107 IWLLCAWVLWTERNNMCFNDSIIPIPRLLDKVKYLSLGWLKTRKASFLFGTFSWWSNPLQ 166
IW+ W LW +RN++ F ++ + LLD ++ S WLK + F + + W S PL+
Sbjct: 196 IWIATVWSLWIQRNSIIFRNNALDHSYLLDLIQSRSWHWLKAKFCGFTYSLYEWKSCPLE 17
Query: 167 CL 168
CL
Sbjct: 16 CL 11
>AV427022
Length = 429
Score = 68.9 bits (167), Expect = 4e-13
Identities = 42/122 (34%), Positives = 61/122 (49%), Gaps = 3/122 (2%)
Frame = +1
Query: 50 ESAQHLFLSCSYFALLWSLVRDWIGFVGVDTNVLSD---HFVQFVHSTGGSKASQSFLQL 106
ES HL SCS L+ W+G V T +SD H +QF S G SKA +
Sbjct: 64 ESGSHLLFSCSNSMLI*YECHAWLG---VSTAQVSDPKVHLLQF-SSIGWSKAQKLGESA 231
Query: 107 IWLLCAWVLWTERNNMCFNDSIIPIPRLLDKVKYLSLGWLKTRKASFLFGTFSWWSNPLQ 166
IW+ W +W RN + FN + +LL++++ + WL++R F + + W SNP
Sbjct: 232 IWMSVLWSIWCLRNMVVFNGGELDKDQLLEQIQVTAWRWLRSRLDDF*YSWYEWKSNPRI 411
Query: 167 CL 168
CL
Sbjct: 412 CL 417
>AV412895
Length = 405
Score = 59.3 bits (142), Expect = 3e-10
Identities = 40/112 (35%), Positives = 55/112 (48%), Gaps = 4/112 (3%)
Frame = +1
Query: 2 IWHIQVPLKVSVFAWRLLRDRLPTKSNLVYRGVIPSEA-GLCVSG*GVLESAQHLFLSCS 60
IW + PL + F WR+L R+ T+ NL+ R VI + +C ES HL SC+
Sbjct: 82 IWMVPAPLNIKAFVWRVLLGRIQTRDNLLKRQVIHNALDAICPLCGLAEESGSHLLFSCA 261
Query: 61 YFALLWSLVRDWIGFVGVDTNVLSD---HFVQFVHSTGGSKASQSFLQLIWL 109
L+W W+ GV T +SD H +QF S G SKA + IW+
Sbjct: 262 ESMLIWYECFAWL---GVSTAQVSDPKVHLLQF-SSIGRSKAQKLGETTIWM 405
>AV428268
Length = 429
Score = 57.8 bits (138), Expect = 1e-09
Identities = 38/125 (30%), Positives = 59/125 (46%), Gaps = 4/125 (3%)
Frame = +3
Query: 1 MIWHIQVPLKVSVFAWRLLRDRLPTKSNLVYRGVIPSE-AGLCVSG*GVLESAQHLFLSC 59
++W ++VP +W++L +R+ TK NL RGV+ S LC ES HL SC
Sbjct: 75 LLWSVKVPSNAIALSWKVLINRVQTKVNLNRRGVVISNVCPLCSLD---EESTDHLLFSC 245
Query: 60 SYFALLWSLVRDWIGFVGVDTNVLSDHFVQFVHSTGGSKASQSFLQ---LIWLLCAWVLW 116
+WS + + G V N HF+Q + GS S +F + +W+ +W
Sbjct: 246 PIV*RIWSKISE*FGVFSVFPNDSHGHFLQHL----GSCGSLNFRKRGWFVWIAAVVCIW 413
Query: 117 TERNN 121
RN+
Sbjct: 414 QGRNS 428
>BP057427
Length = 480
Score = 55.1 bits (131), Expect = 6e-09
Identities = 27/87 (31%), Positives = 42/87 (48%)
Frame = -1
Query: 84 SDHFVQFVHSTGGSKASQSFLQLIWLLCAWVLWTERNNMCFNDSIIPIPRLLDKVKYLSL 143
S +F+QF+ G K + IW+ +W RN++ F + RL+D+++ S
Sbjct: 465 SVNFLQFMQGIGNLKRRRGAWS-IWVAALASIWYSRNSLIFKGEELDFQRLVDQIQTKSW 289
Query: 144 GWLKTRKASFLFGTFSWWSNPLQCLGI 170
WLK + F + W S PL CL I
Sbjct: 288 SWLKAKVTGFSYSLIEWKSQPLSCLDI 208
>TC14968 similar to UP|Q8TTE6 (Q8TTE6) Bacterial extracellular
solute-binding family 3 protein, partial (3%)
Length = 503
Score = 54.3 bits (129), Expect = 1e-08
Identities = 28/104 (26%), Positives = 43/104 (40%)
Frame = +1
Query: 65 LWSLVRDWIGFVGVDTNVLSDHFVQFVHSTGGSKASQSFLQLIWLLCAWVLWTERNNMCF 124
+W V +W+G V DH + F A + + +WL W +W RN F
Sbjct: 91 IWCRVFNWLGVCFVCPGTPVDHLLSFAGLF--LLAQKDGILSVWLAVVWHVWVGRNEAVF 264
Query: 125 NDSIIPIPRLLDKVKYLSLGWLKTRKASFLFGTFSWWSNPLQCL 168
+ + + VK WLK R F T+ W++ P+ CL
Sbjct: 265 REGVFLPECIFYLVKLKVWEWLKARSCHFTHATYEWFNEPILCL 396
>AU089120
Length = 586
Score = 48.9 bits (115), Expect = 5e-07
Identities = 31/106 (29%), Positives = 45/106 (42%), Gaps = 2/106 (1%)
Frame = +2
Query: 50 ESAQHLFLSCSYFALLWSLVRDWIGFVGVDTNVLSDHFVQFVHSTGGSKASQSFLQLIWL 109
ES HLF C LWS V W+ F V HF+ F G + ++ L IWL
Sbjct: 62 ESIPHLFFRCFQSWNLWSSVHRWLVFTVVTPEEGKMHFIMF*DYYSG-RDIKNGLGFIWL 238
Query: 110 LCAWVLWTERNNMCFNDSIIPIPR--LLDKVKYLSLGWLKTRKASF 153
W +W RN + FN + R D + +++ K+ + F
Sbjct: 239 STIWFIWHLRNKVVFNGRCFRLVRGFRFDSISIVAMAKRKSERVMF 376
Score = 35.8 bits (81), Expect = 0.004
Identities = 15/34 (44%), Positives = 19/34 (55%)
Frame = +3
Query: 135 LDKVKYLSLGWLKTRKASFLFGTFSWWSNPLQCL 168
LD ++Y S WLK + F + W SNPL CL
Sbjct: 315 LDSIQYRSWLWLKGKVKGLCFSLYEWSSNPLVCL 416
>TC15601
Length = 613
Score = 48.9 bits (115), Expect = 5e-07
Identities = 19/63 (30%), Positives = 33/63 (52%)
Frame = +2
Query: 107 IWLLCAWVLWTERNNMCFNDSIIPIPRLLDKVKYLSLGWLKTRKASFLFGTFSWWSNPLQ 166
+ + AW LW RN + F + + R+++ + + WL+ +K F F T+ W NP
Sbjct: 83 VCIAIAWTLWNLRNMIIFKEGHLDGERVVEIAQLRAWNWLRAKKKDFKFSTYEWVVNPRY 262
Query: 167 CLG 169
C+G
Sbjct: 263 CIG 271
>AU089037
Length = 245
Score = 47.8 bits (112), Expect = 1e-06
Identities = 26/59 (44%), Positives = 32/59 (54%), Gaps = 1/59 (1%)
Frame = -1
Query: 2 IWHIQVPLKVSVFAWRLLRDRLPTKSNLVYRGVI-PSEAGLCVSG*GVLESAQHLFLSC 59
+W + P FAWRL+ DR+ T+ NL R VI SE LC ES+ HLF SC
Sbjct: 191 LWKVPAPSNAVSFAWRLILDRVQTRGNLRRRQVIQQSEEALCPMCSQCEESSSHLFFSC 15
>BP034765
Length = 506
Score = 47.4 bits (111), Expect = 1e-06
Identities = 21/63 (33%), Positives = 29/63 (45%)
Frame = -2
Query: 106 LIWLLCAWVLWTERNNMCFNDSIIPIPRLLDKVKYLSLGWLKTRKASFLFGTFSWWSNPL 165
LI AW LW RN + FN ++ + DK + WL + F F + W S P+
Sbjct: 505 LI*FAMAWALWEHRNWIVFNGGLLQEDSIFDKAQLKVCQWLSAKGKDFHFSMYEWLSQPI 326
Query: 166 QCL 168
CL
Sbjct: 325 LCL 317
>TC11392 similar to UP|Q8BGI0 (Q8BGI0) Sterol-C5-desaturase, partial (5%)
Length = 579
Score = 43.5 bits (101), Expect = 2e-05
Identities = 18/56 (32%), Positives = 30/56 (53%)
Frame = +3
Query: 113 WVLWTERNNMCFNDSIIPIPRLLDKVKYLSLGWLKTRKASFLFGTFSWWSNPLQCL 168
W L RN++ F+ ++ +LD + + S WLK++ F F W +NPL C+
Sbjct: 27 WSLXLVRNSILFSGGVLDKANILDSIPWRSWFWLKSKMDGFTSSLFEWKTNPLICI 194
>BP033775
Length = 530
Score = 43.1 bits (100), Expect = 2e-05
Identities = 18/54 (33%), Positives = 31/54 (57%)
Frame = -2
Query: 117 TERNNMCFNDSIIPIPRLLDKVKYLSLGWLKTRKASFLFGTFSWWSNPLQCLGI 170
T RN++ FN I ++D +K+ + WL+ + F + +++W NP CLGI
Sbjct: 400 THRNDVLFNGKTINSEAVVDLIKFKAWKWLQCKTKDFSYSSYAWNWNPCICLGI 239
>TC20217
Length = 561
Score = 42.4 bits (98), Expect = 4e-05
Identities = 23/82 (28%), Positives = 34/82 (41%)
Frame = +1
Query: 55 LFLSCSYFALLWSLVRDWIGFVGVDTNVLSDHFVQFVHSTGGSKASQSFLQLIWLLCAWV 114
L SC +W W+G H + F GG+K Q IWL W
Sbjct: 304 LLFSCPVSLDIWRHCYRWMGVCTTLPRNPRQHLL*F--QFGGNKKHQRGADAIWLAVIWT 477
Query: 115 LWTERNNMCFNDSIIPIPRLLD 136
LW RN + F + ++ IP +++
Sbjct: 478 LWLIRNEIIFRNGMLDIPAVME 543
>BP079663
Length = 434
Score = 39.7 bits (91), Expect = 3e-04
Identities = 13/45 (28%), Positives = 26/45 (56%)
Frame = -2
Query: 106 LIWLLCAWVLWTERNNMCFNDSIIPIPRLLDKVKYLSLGWLKTRK 150
++W+ W +WT RN F + + R++D +++ S WL+ +K
Sbjct: 400 VVWVSVIWSIWTMRNACIFKGEVPALDRVMDLIQFRSWSWLREKK 266
>AV411435
Length = 426
Score = 39.3 bits (90), Expect = 4e-04
Identities = 22/66 (33%), Positives = 30/66 (45%), Gaps = 1/66 (1%)
Frame = +3
Query: 2 IWHIQVPLKVSVFAWRLLRDRLPTKSNLVYRGVIPSE-AGLCVSG*GVLESAQHLFLSCS 60
IW QVP KV F WR + +P K NL R + + +C G E H L+C
Sbjct: 177 IWGAQVPKKVRSFLWRAANNAIPVKRNLKRRNMGRDDFCPICNKG---PEDINHALLACE 347
Query: 61 YFALLW 66
+ +W
Sbjct: 348 WTRAVW 365
>BP065246
Length = 505
Score = 39.3 bits (90), Expect = 4e-04
Identities = 16/51 (31%), Positives = 26/51 (50%)
Frame = -2
Query: 119 RNNMCFNDSIIPIPRLLDKVKYLSLGWLKTRKASFLFGTFSWWSNPLQCLG 169
RN + F + + + R D ++ + WLK + F F + W SNP C+G
Sbjct: 447 RNKVIFRNEEVDLERAWDLIQLRAWNWLKAGCSKFHFSIYDWISNPRYCIG 295
>TC16776
Length = 606
Score = 39.3 bits (90), Expect = 4e-04
Identities = 19/61 (31%), Positives = 31/61 (50%)
Frame = +1
Query: 86 HFVQFVHSTGGSKASQSFLQLIWLLCAWVLWTERNNMCFNDSIIPIPRLLDKVKYLSLGW 145
HF+Q GSK L LIW+ ++W RN F + ++ I + +D V++ GW
Sbjct: 64 HFLQHESLLPGSKKVAQTLTLIWVATVGLIWNSRNGAEFREEVVEICKSVDTVQF--KGW 237
Query: 146 L 146
+
Sbjct: 238 V 240
>BP041113
Length = 503
Score = 38.9 bits (89), Expect = 5e-04
Identities = 24/58 (41%), Positives = 29/58 (49%), Gaps = 1/58 (1%)
Frame = +1
Query: 2 IWHIQVPLKVSVFAWRLLRDRLPTKSNLVYRGVIPSEA-GLCVSG*GVLESAQHLFLS 58
IW P V FAWR L RLPT NL+ R V+ A +C G +ES HL +
Sbjct: 331 IWRTVAP-SVKAFAWRCLLGRLPTYDNLIKRQVVVDPAMTVCKFCQGEVESVTHLLFA 501
>TC15307
Length = 589
Score = 30.4 bits (67), Expect(2) = 0.002
Identities = 12/40 (30%), Positives = 18/40 (45%)
Frame = +1
Query: 129 IPIPRLLDKVKYLSLGWLKTRKASFLFGTFSWWSNPLQCL 168
+P + DK + WL + F F + W S P+ CL
Sbjct: 43 LPEDSIFDKAQLKVWQWLSAKGKDFHFSMYEWQSQPILCL 162
Score = 25.4 bits (54), Expect(2) = 0.002
Identities = 8/14 (57%), Positives = 10/14 (71%)
Frame = +2
Query: 112 AWVLWTERNNMCFN 125
AW LW RN +CF+
Sbjct: 2 AWALWVHRNWICFH 43
>TC19293
Length = 472
Score = 36.6 bits (83), Expect = 0.002
Identities = 14/35 (40%), Positives = 21/35 (60%)
Frame = +3
Query: 134 LLDKVKYLSLGWLKTRKASFLFGTFSWWSNPLQCL 168
+ D V++ + WLK + SF + F W SNP +CL
Sbjct: 30 VFDLVQFKAWSWLKAKMRSFHYSLFEWISNPQECL 134
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.331 0.143 0.497
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,442,001
Number of Sequences: 28460
Number of extensions: 74699
Number of successful extensions: 528
Number of sequences better than 10.0: 78
Number of HSP's better than 10.0 without gapping: 520
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 522
length of query: 170
length of database: 4,897,600
effective HSP length: 84
effective length of query: 86
effective length of database: 2,506,960
effective search space: 215598560
effective search space used: 215598560
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.9 bits)
S2: 52 (24.6 bits)
Medicago: description of AC122166.12