BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC122163.6 + phase: 0
(459 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC13701 similar to UP|Q9SIR4 (Q9SIR4) At2g25290 protein, partial... 125 1e-29
TC11960 weakly similar to PIR|T01449|T01449 cytoskeletal protein... 65 3e-11
TC16033 UP|Q7X9C1 (Q7X9C1) NIN-like protein 1, partial (88%) 28 2.4
TC9725 similar to UP|Q8LEF5 (Q8LEF5) 50S ribosomal protein L27, ... 28 3.2
AV408324 27 7.1
TC14106 similar to UP|Q42966 (Q42966) Nitrilase , partial (87%) 27 7.1
BP034914 27 7.1
AV406797 27 7.1
AU240127 27 9.2
CN825309 27 9.2
TC11700 homologue to GB|AAL06971.1|15810091|AY056083 AT4g30260/F... 27 9.2
>TC13701 similar to UP|Q9SIR4 (Q9SIR4) At2g25290 protein, partial (16%)
Length = 432
Score = 125 bits (314), Expect = 1e-29
Identities = 68/138 (49%), Positives = 95/138 (68%), Gaps = 6/138 (4%)
Frame = +1
Query: 225 SDALEETVTSDEAQDLLDKAEFKFQEVAALAFFNWGNVHMCAARKFVRMDENENEVLVMN 284
S A+EE VT+D+AQ+L + A KFQE+AALA FNWGNVH+ AR+ V E+ +
Sbjct: 7 SKAIEEAVTTDDAQELFEIAASKFQEMAALAMFNWGNVHLSRARRRVSFPEDGSREASFE 186
Query: 285 --ESEFDFVQEKYYLAREKYEQAVVIKPDFYEGLLAIGQQQFELAKLNWSFGIANKMDLG 342
++ +++ Q++Y A KYE+A+ IKPDFYEGLLA+G QQFE AKL W + IA+K DL
Sbjct: 187 HVKAAYEWAQKEYKEAELKYEEAMRIKPDFYEGLLALGYQQFEQAKLCWCYLIASKKDLE 366
Query: 343 ----KETLRLFDVAEEKM 356
+E L+L++ AE+ M
Sbjct: 367 AGPFEEVLQLYNKAEDSM 420
>TC11960 weakly similar to PIR|T01449|T01449 cytoskeletal protein homolog
F24O1.11 - Arabidopsis thaliana {Arabidopsis thaliana;},
partial (5%)
Length = 538
Score = 64.7 bits (156), Expect = 3e-11
Identities = 31/63 (49%), Positives = 43/63 (68%), Gaps = 3/63 (4%)
Frame = +3
Query: 400 KLGMSDWKKKLDASVERFKIAGASEADVSGILKKHCFNGNAR---DEKIKGSPRTHNVIK 456
KLGMS W++ LDA+ RFK+AGASE D+S +LK HC NG+A+ D+K + S H
Sbjct: 6 KLGMSGWERNLDAATIRFKLAGASETDISMVLKNHCSNGDAKDGDDQKAQNSTTRHMA*S 185
Query: 457 TMK 459
+M+
Sbjct: 186 SMR 194
>TC16033 UP|Q7X9C1 (Q7X9C1) NIN-like protein 1, partial (88%)
Length = 2554
Score = 28.5 bits (62), Expect = 2.4
Identities = 25/99 (25%), Positives = 40/99 (40%), Gaps = 6/99 (6%)
Frame = +3
Query: 30 PSPKKSDQISRRLREPKLGPTNEH------LKENQKEDVKIAWRQLKLVYDDDIRLAQMP 83
PS K+ R L + LG + LK + K ++K+ Y D+ +MP
Sbjct: 2007 PSENKAQLFPRSLSQETLGEHTKTEYQSYLLKTCHQATPKEDAHRVKVTYGDEKTRFRMP 2186
Query: 84 INCSFRLLRDIVKEKFPISRSVLIKYKDNDDDLVTITST 122
+ S+ L + +F +S K DDDL + T
Sbjct: 2187 KSWSYEHLLQEIARRFNVSDMSKFDVKYLDDDLEWVLLT 2303
>TC9725 similar to UP|Q8LEF5 (Q8LEF5) 50S ribosomal protein L27, partial
(70%)
Length = 846
Score = 28.1 bits (61), Expect = 3.2
Identities = 17/47 (36%), Positives = 25/47 (53%), Gaps = 7/47 (14%)
Frame = -3
Query: 147 EVSPEHEPPLLKEEKEEENNEKQKPLDCVL-------DEKMCTECNK 186
EV E P+ KEE+EEE++E +PL+ V D+ EC +
Sbjct: 142 EVGRSEEEPVKKEEEEEEDSE--RPLNAVAMLKLMHSDDAAMAECGE 8
>AV408324
Length = 309
Score = 26.9 bits (58), Expect = 7.1
Identities = 11/22 (50%), Positives = 14/22 (63%)
Frame = +2
Query: 14 SQPNSPSSSNIAFEPHPSPKKS 35
+ P SP+SS P+P PKKS
Sbjct: 227 ASPASPTSSTTPMTPNPPPKKS 292
>TC14106 similar to UP|Q42966 (Q42966) Nitrilase , partial (87%)
Length = 1286
Score = 26.9 bits (58), Expect = 7.1
Identities = 15/41 (36%), Positives = 21/41 (50%)
Frame = +3
Query: 4 SGSRRKKGNVSQPNSPSSSNIAFEPHPSPKKSDQISRRLRE 44
+G R+ G+ S P P S F+P PS +S RLR+
Sbjct: 6 TGPPRRGGHGSIPTPPPSEPRLFKPPPSSTTLPLLSIRLRD 128
>BP034914
Length = 544
Score = 26.9 bits (58), Expect = 7.1
Identities = 17/45 (37%), Positives = 24/45 (52%), Gaps = 2/45 (4%)
Frame = +3
Query: 2 RRSGSRRKKGNVSQPN--SPSSSNIAFEPHPSPKKSDQISRRLRE 44
++ G +RKK + SPSSS+ + S SD+ SRR RE
Sbjct: 15 KKMGDKRKKQRYVHDDTPSPSSSSSSSTASDSDSDSDRSSRRRRE 149
>AV406797
Length = 418
Score = 26.9 bits (58), Expect = 7.1
Identities = 18/41 (43%), Positives = 21/41 (50%)
Frame = +1
Query: 4 SGSRRKKGNVSQPNSPSSSNIAFEPHPSPKKSDQISRRLRE 44
S SRRK SQP S +SSN P P + IS+R E
Sbjct: 259 SPSRRKPPPPSQPPSSNSSNKPPRPFLPPLQPLSISKRSAE 381
>AU240127
Length = 300
Score = 26.6 bits (57), Expect = 9.2
Identities = 13/47 (27%), Positives = 23/47 (48%)
Frame = +2
Query: 4 SGSRRKKGNVSQPNSPSSSNIAFEPHPSPKKSDQISRRLREPKLGPT 50
+ + N + +P+ + PH +P+ +ISRR +LGPT
Sbjct: 20 TANNNNNNNATAAPTPTQATSQGPPHSAPQS--RISRRRHSVRLGPT 154
>CN825309
Length = 692
Score = 26.6 bits (57), Expect = 9.2
Identities = 18/55 (32%), Positives = 29/55 (52%), Gaps = 3/55 (5%)
Frame = +3
Query: 98 KFPISRSVLIKYKDNDDDLVTI---TSTEELRFAESCVYKTDSVEILKLYIVEVS 149
K PIS S LIKY +D V + T T+++R VY+ ++++ L E +
Sbjct: 180 KPPISLSRLIKYAKGKEDKVVVIVGTVTDDIR-----VYEVPALKVTALRFTETA 329
>TC11700 homologue to GB|AAL06971.1|15810091|AY056083 AT4g30260/F9N11_110
{Arabidopsis thaliana;}, partial (45%)
Length = 561
Score = 26.6 bits (57), Expect = 9.2
Identities = 19/63 (30%), Positives = 26/63 (41%)
Frame = +2
Query: 10 KGNVSQPNSPSSSNIAFEPHPSPKKSDQISRRLREPKLGPTNEHLKENQKEDVKIAWRQL 69
K ++SQP P I+ P P P +IS P E + + K D+ L
Sbjct: 221 KSSISQPQLPKP--ISLPPAPPPSTRPEISSSGFGPAPNTLTEPVWDTVKRDLSRIVSNL 394
Query: 70 KLV 72
KLV
Sbjct: 395 KLV 403
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.316 0.134 0.386
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,131,757
Number of Sequences: 28460
Number of extensions: 85745
Number of successful extensions: 538
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 533
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 537
length of query: 459
length of database: 4,897,600
effective HSP length: 93
effective length of query: 366
effective length of database: 2,250,820
effective search space: 823800120
effective search space used: 823800120
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 57 (26.6 bits)
Medicago: description of AC122163.6