BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC122162.9 - phase: 0
(380 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP028499 74 4e-14
AV422434 61 3e-10
BP069765 51 3e-07
TC15402 UP|CAE45587 (CAE45587) Vacuolar proton-ATPase subunit-li... 30 0.51
BP076687 29 1.1
BP051299 29 1.1
TC9467 weakly similar to UP|Q8WQ86 (Q8WQ86) Microtubule-associat... 29 1.1
BP052653 29 1.1
BI419994 29 1.5
BP035281 29 1.5
AV418517 28 1.9
AV770218 25 2.9
BP041538 28 3.3
TC19408 homologue to PIR|S39523|S39523 coproporphyrinogen oxidas... 28 3.3
AI967453 27 5.6
AV410359 27 5.6
CB826675 27 5.6
TC9053 27 5.6
AV775780 27 5.6
AV775660 27 7.3
>BP028499
Length = 491
Score = 73.9 bits (180), Expect = 4e-14
Identities = 49/127 (38%), Positives = 68/127 (52%), Gaps = 13/127 (10%)
Frame = +2
Query: 2 AEADWSQLPKDLLTLISQRIDTEIDLIRFRSICSNWRSSS----IPNHHNILPFKFPLLK 57
A DWSQLP +LL LISQR+++ + L+RFRS+CS WR SS IP HH PF FP L
Sbjct: 107 APPDWSQLPAELLHLISQRLNSPLYLLRFRSVCSTWRHSSASSFIPIHH--FPFNFPPLS 280
Query: 58 CEDNSIKYNDNEISSSSNH-----HPSQLEEKQTLIPRRPWLIRFTQN----SHGKTKLF 108
D ++D S + P QT + PWL++ ++ +T+L+
Sbjct: 281 DHD----HHDASSFSLTKRTVVLITPPPPPHHQT---QTPWLVKIGEDLCDGDRTRTRLW 439
Query: 109 HPLIKYP 115
HPL + P
Sbjct: 440 HPLFRGP 460
>AV422434
Length = 422
Score = 61.2 bits (147), Expect = 3e-10
Identities = 37/96 (38%), Positives = 53/96 (54%), Gaps = 8/96 (8%)
Frame = +3
Query: 5 DWSQLPKDLLTLISQRIDTEIDLIRFRSICSNWRSSS----IPNHHNILPFKFPLLKCED 60
DWSQLP +LL LISQR+D+ + L+RFRS+CS WR SS P HH F PL +
Sbjct: 57 DWSQLPTELLHLISQRLDSPLYLLRFRSVCSTWRRSSSSAVTPLHH----FPNPLRRHRF 224
Query: 61 NSIKYNDNEISSSSNHHPSQLEEKQT----LIPRRP 92
+ + + + HHP+ +T ++P +P
Sbjct: 225 HLLPLPTHRLP----HHPTATPTNRTPNPLVVPNQP 320
>BP069765
Length = 417
Score = 51.2 bits (121), Expect = 3e-07
Identities = 27/60 (45%), Positives = 36/60 (60%)
Frame = -2
Query: 298 KKWVKLRNFGDRVFFIGRGCSFSASASDLCIQKGNCAIFIDESVLHNNNMVRGKRVFHLD 357
+ WV +RN DRV FIG C+FS SASDL + +GN +F D+ ++ G VF LD
Sbjct: 416 ESWVHVRNVRDRVLFIGDDCAFSVSASDLGVDEGNYVVFRDD------DLKSGIGVFPLD 255
>TC15402 UP|CAE45587 (CAE45587) Vacuolar proton-ATPase subunit-like
protein, complete
Length = 2423
Score = 30.4 bits (67), Expect = 0.51
Identities = 13/38 (34%), Positives = 21/38 (55%)
Frame = +3
Query: 56 LKCEDNSIKYNDNEISSSSNHHPSQLEEKQTLIPRRPW 93
L D S+ + + ++ S HHP ++ L+PRRPW
Sbjct: 27 LLSSDGSVPFRAD--ATRSTHHPHRICSPHCLLPRRPW 134
>BP076687
Length = 389
Score = 29.3 bits (64), Expect = 1.1
Identities = 13/33 (39%), Positives = 21/33 (63%)
Frame = -3
Query: 7 SQLPKDLLTLISQRIDTEIDLIRFRSICSNWRS 39
S LP++L+ I R+ L+RF+S+C +W S
Sbjct: 387 SHLPEELIPQILLRLPVR-SLLRFKSVCKSWLS 292
>BP051299
Length = 539
Score = 29.3 bits (64), Expect = 1.1
Identities = 18/60 (30%), Positives = 30/60 (50%), Gaps = 5/60 (8%)
Frame = +2
Query: 9 LPKDLLTLISQRIDTEIDLIRFRSICSNWRS-----SSIPNHHNILPFKFPLLKCEDNSI 63
LP+++ I R+ + L++ S+C +WRS S I H N P L C++ +I
Sbjct: 5 LPQEIWAEILHRLPPKT-LVKCTSVCKSWRSLITSTSFISLHRNHSPSSLLLQLCDERAI 181
>TC9467 weakly similar to UP|Q8WQ86 (Q8WQ86) Microtubule-associated protein
EB1, partial (7%)
Length = 593
Score = 29.3 bits (64), Expect = 1.1
Identities = 19/53 (35%), Positives = 23/53 (42%)
Frame = +2
Query: 75 NHHPSQLEEKQTLIPRRPWLIRFTQNSHGKTKLFHPLIKYPRPQQEETLAHPR 127
NHH +QT +P R SH L PL +PR +Q L HPR
Sbjct: 263 NHHRRSHTLQQTPLPLR---------SHHHLLLPRPLQTHPRRRQHPELQHPR 394
>BP052653
Length = 552
Score = 29.3 bits (64), Expect = 1.1
Identities = 14/33 (42%), Positives = 20/33 (60%)
Frame = +1
Query: 7 SQLPKDLLTLISQRIDTEIDLIRFRSICSNWRS 39
S LP DL+ I R+ L+RF+S+C +W S
Sbjct: 181 SILPHDLIVQILLRLPVR-SLLRFKSVCKSWLS 276
>BI419994
Length = 549
Score = 28.9 bits (63), Expect = 1.5
Identities = 12/33 (36%), Positives = 21/33 (63%)
Frame = +2
Query: 6 WSQLPKDLLTLISQRIDTEIDLIRFRSICSNWR 38
+S LP +L+ I R+ L+RF+++C +WR
Sbjct: 38 FSHLPDELILQILLRLPVR-SLLRFKTVCKSWR 133
>BP035281
Length = 498
Score = 28.9 bits (63), Expect = 1.5
Identities = 16/51 (31%), Positives = 29/51 (56%)
Frame = +2
Query: 25 IDLIRFRSICSNWRSSSIPNHHNILPFKFPLLKCEDNSIKYNDNEISSSSN 75
+ LIRF+ +C +W+ S+ +H+ F L+ E S+K N ++SS +
Sbjct: 167 LSLIRFKCVCKSWK--SLISHNK----AFAKLQLERTSLKINHVILTSSDS 301
>AV418517
Length = 298
Score = 28.5 bits (62), Expect = 1.9
Identities = 16/43 (37%), Positives = 25/43 (57%)
Frame = -3
Query: 36 NWRSSSIPNHHNILPFKFPLLKCEDNSIKYNDNEISSSSNHHP 78
N+ SSI NHH + PF +L D+ + + ++SS+N HP
Sbjct: 182 NFLLSSIHNHHLLTPFCHQML---DHKLSH----LASSNNQHP 75
>AV770218
Length = 567
Score = 24.6 bits (52), Expect(2) = 2.9
Identities = 9/15 (60%), Positives = 11/15 (73%)
Frame = -1
Query: 264 LGIEESSNYFSIDFF 278
LG++ S NY IDFF
Sbjct: 264 LGVKPSGNYLDIDFF 220
Score = 21.6 bits (44), Expect(2) = 2.9
Identities = 10/27 (37%), Positives = 14/27 (51%)
Frame = -2
Query: 313 IGRGCSFSASASDLCIQKGNCAIFIDE 339
I C FS AS +C+ N +ID+
Sbjct: 170 ISHFCCFSVHASFVCMNVCNIFNYIDQ 90
>BP041538
Length = 375
Score = 27.7 bits (60), Expect = 3.3
Identities = 14/36 (38%), Positives = 21/36 (57%)
Frame = -2
Query: 264 LGIEESSNYFSIDFFELDEKKKKWVRLMDFDEKEKK 299
LG+E + + D+ +KKKKW+R + FD K K
Sbjct: 167 LGLEAGGGWRTADWGRRRKKKKKWLR-VGFDRKGPK 63
>TC19408 homologue to PIR|S39523|S39523 coproporphyrinogen oxidase
precursor - soybean {Glycine max;} , partial (19%)
Length = 593
Score = 27.7 bits (60), Expect = 3.3
Identities = 13/29 (44%), Positives = 19/29 (64%)
Frame = -1
Query: 211 TCVFKGQLYAVHQSGQTVTIGPDSSVELA 239
TCV KG LY + +G T G DSS++++
Sbjct: 494 TCVHKGFLYYLLNNGGHSTWGTDSSLDMS 408
>AI967453
Length = 456
Score = 26.9 bits (58), Expect = 5.6
Identities = 23/74 (31%), Positives = 35/74 (47%), Gaps = 3/74 (4%)
Frame = +1
Query: 13 LLTLISQRIDTEIDLIRFRSICSNWRSSSIPNHHNILPFKFP---LLKCEDNSIKYNDNE 69
L+T I R+ + L+RF+++C WRS H F+ LL D+ I+ D E
Sbjct: 16 LITEILLRLPVK-SLVRFKAVCKFWRSLISDPHFATSHFERAAPRLLIRTDHGIRTMDLE 192
Query: 70 ISSSSNHHPSQLEE 83
S HP ++ E
Sbjct: 193ESL----HPDRISE 222
>AV410359
Length = 342
Score = 26.9 bits (58), Expect = 5.6
Identities = 9/13 (69%), Positives = 12/13 (92%)
Frame = +2
Query: 27 LIRFRSICSNWRS 39
LIRF+S+C +WRS
Sbjct: 11 LIRFKSVCKSWRS 49
>CB826675
Length = 551
Score = 26.9 bits (58), Expect = 5.6
Identities = 13/28 (46%), Positives = 14/28 (49%)
Frame = +1
Query: 312 FIGRGCSFSASASDLCIQKGNCAIFIDE 339
FIG G A L +K C IFIDE
Sbjct: 358 FIGDGAKLVRDAFQLAKEKSPCIIFIDE 441
>TC9053
Length = 618
Score = 26.9 bits (58), Expect = 5.6
Identities = 20/70 (28%), Positives = 32/70 (45%), Gaps = 14/70 (20%)
Frame = -1
Query: 44 NHHNILPFKFPLLKCEDN------SIKYNDNEIS-SSSNHHPSQLEEKQ-------TLIP 89
N + L F KC N S KYN NEI+ ++S+HH + K ++P
Sbjct: 600 NFQSCLFLFFAARKCHSNH*LIISSSKYNSNEITKTTSSHHTKMIMTKNIEKNGT*LMVP 421
Query: 90 RRPWLIRFTQ 99
+ P ++T+
Sbjct: 420 KFPRPNKYTR 391
>AV775780
Length = 502
Score = 26.9 bits (58), Expect = 5.6
Identities = 15/57 (26%), Positives = 26/57 (45%), Gaps = 13/57 (22%)
Frame = -2
Query: 4 ADWSQLPKDLLTLISQRIDTEIDLIRFRSICS----NW---------RSSSIPNHHN 47
A W+ L TL+ ++ + +R R++CS W S S+P++HN
Sbjct: 345 ASWTPLHAPAATLVPRQTAVVVSRVRSRNLCSRCTRRW*ISKEVPQVPSQSVPSYHN 175
>AV775660
Length = 487
Score = 26.6 bits (57), Expect = 7.3
Identities = 17/49 (34%), Positives = 24/49 (48%), Gaps = 1/49 (2%)
Frame = +3
Query: 39 SSSIPNHHNILPFKFPLLKCEDNSIKY-NDNEISSSSNHHPSQLEEKQT 86
SSS P+HH L F P LK +D + N E + N P+ + + T
Sbjct: 147 SSSSPSHHLHLIFSIPKLKNQDLEPQS*NGYEQLNLKNQTPNNIPDSHT 293
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.322 0.139 0.432
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,271,540
Number of Sequences: 28460
Number of extensions: 137560
Number of successful extensions: 690
Number of sequences better than 10.0: 45
Number of HSP's better than 10.0 without gapping: 688
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 690
length of query: 380
length of database: 4,897,600
effective HSP length: 92
effective length of query: 288
effective length of database: 2,279,280
effective search space: 656432640
effective search space used: 656432640
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 56 (26.2 bits)
Medicago: description of AC122162.9