BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC122162.7 - phase: 0
(377 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP028499 88 2e-18
AV422434 63 7e-11
BP069765 57 4e-09
BI419994 34 0.035
BP076687 34 0.035
AV414925 31 0.39
BP051299 30 0.86
BP052653 30 0.86
TC16084 similar to GB|AAL84954.1|19310435|AY078954 F17H15.1/F17H... 29 1.5
TC11750 similar to UP|Q8L9B8 (Q8L9B8) Transaldolase ToTAL2, part... 29 1.5
BP038938 28 2.5
BP035281 28 2.5
TC13166 similar to UP|Q94G16 (Q94G16) HCF106, partial (20%) 27 4.3
TC18736 similar to UP|Q40485 (Q40485) C1C-Nt1 protein, partial (... 27 4.3
TC7956 similar to GB|AAM61419.1|21537078|AY084855 putaive DNA-bi... 27 4.3
AV423520 27 5.6
TC14718 similar to UP|NCPR_CATRO (Q05001) NADPH--cytochrome P450... 27 5.6
AV410359 27 7.3
TC14476 homologue to UP|TBB3_WHEAT (Q9ZRB0) Tubulin beta-3 chain... 27 7.3
TC9827 weakly similar to UP|Q8S9A2 (Q8S9A2) Glucosyltransferase-... 27 7.3
>BP028499
Length = 491
Score = 88.2 bits (217), Expect = 2e-18
Identities = 52/122 (42%), Positives = 68/122 (55%), Gaps = 4/122 (3%)
Frame = +2
Query: 3 DWSHLPSELLQLISQKLDTVLCLLRFRSVCSTWRSSSIPNHKHKNSSPLNIPQFPEPNFD 62
DWS LP+ELL LISQ+L++ L LLRFRSVCSTWR SS SS + I FP NF
Sbjct: 116 DWSQLPAELLHLISQRLNSPLYLLRFRSVCSTWRHSSA-------SSFIPIHHFPF-NFP 271
Query: 63 PFNIRNIIKSKYITSRLITHTIFLIKPPQHQPSLNPWLIRIGPDF----KGKTQLWHPFS 118
P + + + + T + PP H + PWL++IG D + +T+LWHP
Sbjct: 272 PLSDHDHHDASSFSLTKRTVVLITPPPPPHHQTQTPWLVKIGEDLCDGDRTRTRLWHPLF 451
Query: 119 SG 120
G
Sbjct: 452 RG 457
>AV422434
Length = 422
Score = 63.2 bits (152), Expect = 7e-11
Identities = 33/57 (57%), Positives = 39/57 (67%)
Frame = +3
Query: 3 DWSHLPSELLQLISQKLDTVLCLLRFRSVCSTWRSSSIPNHKHKNSSPLNIPQFPEP 59
DWS LP+ELL LISQ+LD+ L LLRFRSVCSTWR SS +S+ + FP P
Sbjct: 57 DWSQLPTELLHLISQRLDSPLYLLRFRSVCSTWRRSS-------SSAVTPLHHFPNP 206
Score = 34.3 bits (77), Expect = 0.035
Identities = 34/113 (30%), Positives = 52/113 (45%), Gaps = 12/113 (10%)
Frame = +1
Query: 36 RSSSI--PN----HKHKNSSPLNIPQ---FPEPNFDPFNIRNIIKSKYITSRLITH-TIF 85
RSSSI PN ++S ++P P P PF + ++ ++ T+F
Sbjct: 76 RSSSI*FPNASIARSTSSASAPSVPHGAALPHPPSRPFTTFPTLSDDTASTFFLSQRTVF 255
Query: 86 LIKPPQHQPS--LNPWLIRIGPDFKGKTQLWHPFSSGQNLSSDFNNVLDFNKL 136
LI PP QP+ L P L +I D +L+ P SS ++ + VL+F L
Sbjct: 256 LIIPPPLQPTEPLTPLLFQISQDNG*TRRLYDPLSSHRDPPFSSSRVLNFYNL 414
>BP069765
Length = 417
Score = 57.4 bits (137), Expect = 4e-09
Identities = 35/87 (40%), Positives = 47/87 (53%), Gaps = 5/87 (5%)
Frame = -2
Query: 290 WVKLANLGDKVLFLGNGYSFSASASELGFANGNCVIYSKSYGFHDLNIRKCKMSVFHLDQ 349
WV + N+ D+VLF+G+ +FS SAS+LG GN V++ DL K + VF LD
Sbjct: 410 WVHVRNVRDRVLFIGDDCAFSVSASDLGVDEGNYVVFRDD----DL---KSGIGVFPLDD 252
Query: 350 -----GQASPLSDYPEYFKLFWPPPEW 371
L DYP + LF PPP+W
Sbjct: 251 VGWYGDGILHLLDYPGFSSLFRPPPDW 171
>BI419994
Length = 549
Score = 34.3 bits (77), Expect = 0.035
Identities = 17/33 (51%), Positives = 23/33 (69%)
Frame = +2
Query: 4 WSHLPSELLQLISQKLDTVLCLLRFRSVCSTWR 36
+SHLP EL+ I +L V LLRF++VC +WR
Sbjct: 38 FSHLPDELILQILLRLP-VRSLLRFKTVCKSWR 133
>BP076687
Length = 389
Score = 34.3 bits (77), Expect = 0.035
Identities = 18/33 (54%), Positives = 22/33 (66%)
Frame = -3
Query: 5 SHLPSELLQLISQKLDTVLCLLRFRSVCSTWRS 37
SHLP EL+ I +L V LLRF+SVC +W S
Sbjct: 387 SHLPEELIPQILLRLP-VRSLLRFKSVCKSWLS 292
>AV414925
Length = 414
Score = 30.8 bits (68), Expect = 0.39
Identities = 23/65 (35%), Positives = 32/65 (48%), Gaps = 3/65 (4%)
Frame = +1
Query: 7 LPSELLQLISQKLDTVLCLLRFRSVCSTWRSSSIPNH---KHKNSSPLNIPQFPEPNFDP 63
+P E++ I +L V LLRFRS+ +WRS H H N+S N P PN
Sbjct: 97 IPVEVIADILSRLP-VTSLLRFRSISKSWRSLIDSKHFMKLHLNNSLTN----PNPNLTN 261
Query: 64 FNIRN 68
+R+
Sbjct: 262 LILRH 276
>BP051299
Length = 539
Score = 29.6 bits (65), Expect = 0.86
Identities = 23/70 (32%), Positives = 35/70 (49%), Gaps = 8/70 (11%)
Frame = +2
Query: 6 HLPSELLQLISQKLDTVLCLLRFRSVCSTWRS-----SSIPNHKHKNSSPLNIPQFPE-- 58
HLP E+ I +L L++ SVC +WRS S I H++ + S L + E
Sbjct: 2 HLPQEIWAEILHRLPPKT-LVKCTSVCKSWRSLITSTSFISLHRNHSPSSLLLQLCDERA 178
Query: 59 -PNFDPFNIR 67
PNF +++R
Sbjct: 179IPNFIHYSLR 208
>BP052653
Length = 552
Score = 29.6 bits (65), Expect = 0.86
Identities = 21/58 (36%), Positives = 30/58 (51%)
Frame = +1
Query: 5 SHLPSELLQLISQKLDTVLCLLRFRSVCSTWRSSSIPNHKHKNSSPLNIPQFPEPNFD 62
S LP +L+ I +L V LLRF+SVC +W S ++ PQF + +FD
Sbjct: 181 SILPHDLIVQILLRLP-VRSLLRFKSVCKSWLSL------------ISDPQFGKSHFD 315
>TC16084 similar to GB|AAL84954.1|19310435|AY078954 F17H15.1/F17H15.1
{Arabidopsis thaliana;}, partial (29%)
Length = 889
Score = 28.9 bits (63), Expect = 1.5
Identities = 23/82 (28%), Positives = 32/82 (38%), Gaps = 7/82 (8%)
Frame = +2
Query: 23 LCLLRFRSVCSTWRSSSIPNHKHKN-------SSPLNIPQFPEPNFDPFNIRNIIKSKYI 75
LCL R VCS W+ H+H+N S P P P P + K
Sbjct: 2 LCLSR---VCSRWQRKRTMQHRHRNPSNGSTMSHPPTAPPASPPLIPPSPTSSSPSRKLK 172
Query: 76 TSRLITHTIFLIKPPQHQPSLN 97
S L++ + L+ P +LN
Sbjct: 173KSLLVSSAVLLL-PQSTSNALN 235
>TC11750 similar to UP|Q8L9B8 (Q8L9B8) Transaldolase ToTAL2, partial (27%)
Length = 536
Score = 28.9 bits (63), Expect = 1.5
Identities = 22/82 (26%), Positives = 35/82 (41%), Gaps = 5/82 (6%)
Frame = +2
Query: 293 LANLGDKVLFLGNGYSF-----SASASELGFANGNCVIYSKSYGFHDLNIRKCKMSVFHL 347
L +L + V F YSF SAS F++ + + ++ + ++ L
Sbjct: 83 LQSLNESVAFPDEKYSFVRRLSPESASRYLFSDEELIKWDQTSLTEAMGPAAVQLLAAGL 262
Query: 348 DQGQASPLSDYPEYFKLFWPPP 369
D G A + EYF+ WPPP
Sbjct: 263 D-GYADQATRVEEYFRKIWPPP 325
>BP038938
Length = 583
Score = 28.1 bits (61), Expect = 2.5
Identities = 16/40 (40%), Positives = 21/40 (52%), Gaps = 3/40 (7%)
Frame = +3
Query: 1 MADWSHLPSELLQLISQKL---DTVLCLLRFRSVCSTWRS 37
+ WS LP+ELL+L+ +L D V R VC W S
Sbjct: 126 LQSWSDLPAELLELVMTRLALDDNV----RASVVCKRWHS 233
>BP035281
Length = 498
Score = 28.1 bits (61), Expect = 2.5
Identities = 15/30 (50%), Positives = 20/30 (66%)
Frame = +2
Query: 8 PSELLQLISQKLDTVLCLLRFRSVCSTWRS 37
PSEL+ I L VL L+RF+ VC +W+S
Sbjct: 125 PSELVIEILSWLP-VLSLIRFKCVCKSWKS 211
>TC13166 similar to UP|Q94G16 (Q94G16) HCF106, partial (20%)
Length = 504
Score = 27.3 bits (59), Expect = 4.3
Identities = 15/27 (55%), Positives = 19/27 (69%), Gaps = 2/27 (7%)
Frame = -1
Query: 11 LLQLISQKLDTVLCLLR--FRSVCSTW 35
LL L S+ L +LC L+ FR+VCSTW
Sbjct: 117 LLLLCSRCL*LLLCNLKILFRAVCSTW 37
>TC18736 similar to UP|Q40485 (Q40485) C1C-Nt1 protein, partial (15%)
Length = 815
Score = 27.3 bits (59), Expect = 4.3
Identities = 12/35 (34%), Positives = 16/35 (45%)
Frame = -1
Query: 35 WRSSSIPNHKHKNSSPLNIPQFPEPNFDPFNIRNI 69
WRS+ I N S N+ FP+P F N +
Sbjct: 212 WRSTYISNSSSSKSRSSNLIPFPDPAFPKSNTETL 108
>TC7956 similar to GB|AAM61419.1|21537078|AY084855 putaive DNA-binding
protein {Arabidopsis thaliana;} , partial (45%)
Length = 1257
Score = 27.3 bits (59), Expect = 4.3
Identities = 19/66 (28%), Positives = 29/66 (43%)
Frame = +3
Query: 29 RSVCSTWRSSSIPNHKHKNSSPLNIPQFPEPNFDPFNIRNIIKSKYITSRLITHTIFLIK 88
R + S SSS P+ H+N++ N+ +F +++I T H F
Sbjct: 147 RILSSQTPSSSTPSSSHRNNNNNNLDYSEITDFTVSKFKHVINLLNRTG----HARFRRA 314
Query: 89 PPQHQP 94
PPQ QP
Sbjct: 315 PPQPQP 332
>AV423520
Length = 468
Score = 26.9 bits (58), Expect = 5.6
Identities = 21/48 (43%), Positives = 25/48 (51%), Gaps = 4/48 (8%)
Frame = +3
Query: 7 LPSELLQLISQKLDTVLCLLRFRSVCSTWRS--SSIPN--HKHKNSSP 50
LP EL+ I +L V LL+FR VC +W S S P KH SP
Sbjct: 165 LPFELVVEILCRLP-VKSLLQFRCVCKSWNSLISGDPKFARKHLRCSP 305
>TC14718 similar to UP|NCPR_CATRO (Q05001) NADPH--cytochrome P450 reductase
(CPR) (P450R) , partial (36%)
Length = 1163
Score = 26.9 bits (58), Expect = 5.6
Identities = 10/21 (47%), Positives = 14/21 (66%)
Frame = +1
Query: 29 RSVCSTWRSSSIPNHKHKNSS 49
R VCSTW +S+P K ++ S
Sbjct: 208 RGVCSTWMKNSVPLEKSQDCS 270
>AV410359
Length = 342
Score = 26.6 bits (57), Expect = 7.3
Identities = 9/13 (69%), Positives = 12/13 (92%)
Frame = +2
Query: 25 LLRFRSVCSTWRS 37
L+RF+SVC +WRS
Sbjct: 11 LIRFKSVCKSWRS 49
>TC14476 homologue to UP|TBB3_WHEAT (Q9ZRB0) Tubulin beta-3 chain (Beta-3
tubulin), complete
Length = 2208
Score = 26.6 bits (57), Expect = 7.3
Identities = 13/46 (28%), Positives = 22/46 (47%)
Frame = -1
Query: 136 LSFRVLGCMSYFYLHHLIGGYQSSFIHCVAAVCRGEQPPVVVTCGE 181
L+ +++GC + H L+ QS C A + PP+ +C E
Sbjct: 1152 LTTQLVGCHKHLTSHCLVSTPQSMMSSCSARLSSVGPPPLCSSCHE 1015
>TC9827 weakly similar to UP|Q8S9A2 (Q8S9A2) Glucosyltransferase-7
(Fragment), partial (42%)
Length = 611
Score = 26.6 bits (57), Expect = 7.3
Identities = 22/84 (26%), Positives = 31/84 (36%), Gaps = 1/84 (1%)
Frame = -2
Query: 115 HPFSSGQNLSSDFNNVLDFNKLSFRVLGCMSYFYLHHLIGGYQSSFIHCVAAVCRGEQPP 174
HPF ++ F + N + +L C S YL + SSF H Q P
Sbjct: 334 HPFDG---IALYFIYLFSLNNFLYAILNCFSSHYLFLISNSSDSSFPHI------NNQLP 182
Query: 175 VVVTCGEFTHCD-INQGMMMFRCG 197
+ HC+ N F+CG
Sbjct: 181 ICDWVSPQWHCNHWNP*AHRFQCG 110
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.324 0.140 0.458
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,051,995
Number of Sequences: 28460
Number of extensions: 158723
Number of successful extensions: 769
Number of sequences better than 10.0: 41
Number of HSP's better than 10.0 without gapping: 760
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 766
length of query: 377
length of database: 4,897,600
effective HSP length: 92
effective length of query: 285
effective length of database: 2,279,280
effective search space: 649594800
effective search space used: 649594800
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 56 (26.2 bits)
Medicago: description of AC122162.7