BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC122162.5 + phase: 1 /partial
(95 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC12113 weakly similar to GB|CAD40580.1|21741678|OSJN00141 oj000... 131 2e-32
BP047703 80 2e-22
BP066300 69 1e-13
TC14758 similar to UP|O24601 (O24601) Glycine-rich RNA binding p... 59 1e-10
TC17853 similar to UP|Q42412 (Q42412) RNA-binding protein RZ-1, ... 59 1e-10
BI419822 58 2e-10
TC15623 similar to UP|P93486 (P93486) Glycine-rich RNA-binding p... 53 8e-09
TC14147 similar to UP|ROC1_NICSY (Q08935) 29 kDa ribonucleoprote... 52 2e-08
TC12531 similar to UP|Q9ASQ8 (Q9ASQ8) At1g22910/F19G10_13, parti... 49 1e-07
TC14336 similar to UP|Q9M549 (Q9M549) Poly(A)-binding protein, p... 49 1e-07
TC8015 similar to UP|ROC4_NICSY (P19683) 31 kDa ribonucleoprotei... 49 1e-07
TC14420 similar to UP|ROC3_NICSY (P19682) 28 kDa ribonucleoprote... 49 1e-07
TC16457 similar to UP|Q41124 (Q41124) Chloroplast RNA binding pr... 48 2e-07
TC19849 similar to UP|Q9AT32 (Q9AT32) Poly(A)-binding protein, p... 47 4e-07
TC14416 similar to UP|Q8SMH8 (Q8SMH8) RNA-binding protein, parti... 46 1e-06
AV773507 45 2e-06
BP047377 45 2e-06
TC11829 weakly similar to UP|NAM8_YEAST (Q00539) NAM8 protein, p... 44 5e-06
TC14392 similar to UP|Q9LEB4 (Q9LEB4) RNA binding protein 45, pa... 44 6e-06
TC10587 weakly similar to GB|AAC50895.1|1518802|HSU63289 CUG-BP/... 43 1e-05
>TC12113 weakly similar to GB|CAD40580.1|21741678|OSJN00141 oj000126_13.2
{Oryza sativa (japonica cultivar-group);} , partial
(40%)
Length = 484
Score = 131 bits (330), Expect = 2e-32
Identities = 63/86 (73%), Positives = 75/86 (86%)
Frame = +1
Query: 1 LAFSTTEEKLAEAFSQYGNVVKADIVLNKAKKRCKGFGYVTFAEEEEARKAQIGMNGKIL 60
LAFSTTE+KLAEAFSQYG V+KADIVLN+AK R KGFGYVTFA+EE+A+KAQIGMN KIL
Sbjct: 22 LAFSTTEDKLAEAFSQYGKVLKADIVLNRAKTRSKGFGYVTFAKEEDAQKAQIGMNRKIL 201
Query: 61 HGRVLYVDMDPPNEQKNKIKQATKNT 86
HGRVLYVDM+PP++ K I + ++
Sbjct: 202 HGRVLYVDMEPPDQHKKSISEGATDS 279
>BP047703
Length = 586
Score = 80.5 bits (197), Expect(2) = 2e-22
Identities = 38/49 (77%), Positives = 45/49 (91%)
Frame = -1
Query: 11 AEAFSQYGNVVKADIVLNKAKKRCKGFGYVTFAEEEEARKAQIGMNGKI 59
AEAFSQYG V+KADIVLN+AK R +GFGYVTFA+EE+A+KAQIGMN K+
Sbjct: 586 AEAFSQYGKVLKADIVLNRAKTRSQGFGYVTFAKEEDAQKAQIGMNRKV 440
Score = 38.5 bits (88), Expect(2) = 2e-22
Identities = 15/29 (51%), Positives = 22/29 (75%)
Frame = -3
Query: 58 KILHGRVLYVDMDPPNEQKNKIKQATKNT 86
+ILHGRVLYVDM+PP++ K I + ++
Sbjct: 335 QILHGRVLYVDMEPPDKHKKSISEGATDS 249
>BP066300
Length = 420
Score = 68.9 bits (167), Expect = 1e-13
Identities = 31/75 (41%), Positives = 49/75 (65%)
Frame = -3
Query: 1 LAFSTTEEKLAEAFSQYGNVVKADIVLNKAKKRCKGFGYVTFAEEEEARKAQIGMNGKIL 60
L+F TTEE L F +G +V +V++K R +GF ++ +A EEE++KA GM+GK L
Sbjct: 412 LSFRTTEESLRNCFKNFGQLVDVXLVMDKLANRPRGFAFLRYATEEESQKAIEGMHGKFL 233
Query: 61 HGRVLYVDMDPPNEQ 75
GRV++V++ P +
Sbjct: 232 DGRVIFVEVAKPRSE 188
>TC14758 similar to UP|O24601 (O24601) Glycine-rich RNA binding protein 2,
partial (93%)
Length = 725
Score = 59.3 bits (142), Expect = 1e-10
Identities = 29/68 (42%), Positives = 42/68 (61%)
Frame = +1
Query: 1 LAFSTTEEKLAEAFSQYGNVVKADIVLNKAKKRCKGFGYVTFAEEEEARKAQIGMNGKIL 60
LA++T L +AFS YG ++ + +V ++ R +GFG+VTF EE R A GMNG L
Sbjct: 103 LAWTTDSHTLEQAFSSYGEIIDSKVVNDRETGRSRGFGFVTFTSEEAMRSAIEGMNGNEL 282
Query: 61 HGRVLYVD 68
GR + V+
Sbjct: 283 DGRNITVN 306
>TC17853 similar to UP|Q42412 (Q42412) RNA-binding protein RZ-1, partial
(58%)
Length = 881
Score = 58.9 bits (141), Expect = 1e-10
Identities = 28/71 (39%), Positives = 46/71 (64%)
Frame = +2
Query: 2 AFSTTEEKLAEAFSQYGNVVKADIVLNKAKKRCKGFGYVTFAEEEEARKAQIGMNGKILH 61
A+ST++ KL +AF ++G + +A +V++K R +GFG+VTF +++ +A MNG L
Sbjct: 80 AWSTSDRKLKDAFEKFGKLTEAKVVVDKFSGRSRGFGFVTFDDKKAMDEAIDAMNGMDLD 259
Query: 62 GRVLYVDMDPP 72
GR + VD P
Sbjct: 260 GRTITVDKAQP 292
>BI419822
Length = 380
Score = 58.2 bits (139), Expect = 2e-10
Identities = 28/68 (41%), Positives = 45/68 (66%)
Frame = +2
Query: 1 LAFSTTEEKLAEAFSQYGNVVKADIVLNKAKKRCKGFGYVTFAEEEEARKAQIGMNGKIL 60
LA++T E L +AFS YG +V++ ++ ++ R +GFG+VTFA E+ + A MNG+ L
Sbjct: 98 LAWATDNEALEKAFSPYGEIVESKVINDRETGRSRGFGFVTFASEQAMKDAIEAMNGQNL 277
Query: 61 HGRVLYVD 68
GR + V+
Sbjct: 278 DGRNITVN 301
>TC15623 similar to UP|P93486 (P93486) Glycine-rich RNA-binding protein
PsGRBP, partial (88%)
Length = 683
Score = 53.1 bits (126), Expect = 8e-09
Identities = 24/67 (35%), Positives = 43/67 (63%)
Frame = +1
Query: 1 LAFSTTEEKLAEAFSQYGNVVKADIVLNKAKKRCKGFGYVTFAEEEEARKAQIGMNGKIL 60
L++ ++ L +AFS +G V +A +++++ R +GFG+V+F EE A A M+G+ L
Sbjct: 205 LSYGVDDKSLEDAFSSFGTVAEARVIVDRDTGRSRGFGFVSFDSEESASSALSSMDGQDL 384
Query: 61 HGRVLYV 67
+GR + V
Sbjct: 385 NGRNIRV 405
>TC14147 similar to UP|ROC1_NICSY (Q08935) 29 kDa ribonucleoprotein A,
chloroplast precursor (CP29A), partial (63%)
Length = 1602
Score = 51.6 bits (122), Expect = 2e-08
Identities = 27/78 (34%), Positives = 39/78 (49%)
Frame = +3
Query: 1 LAFSTTEEKLAEAFSQYGNVVKADIVLNKAKKRCKGFGYVTFAEEEEARKAQIGMNGKIL 60
L FS +LAE F GNV +++ +K +GF +VT + EA A N L
Sbjct: 366 LPFSVDSAQLAELFQDAGNVEVVEVIYDKMTGNSRGFAFVTMSSAAEAEAAAQQFNNYEL 545
Query: 61 HGRVLYVDMDPPNEQKNK 78
GR L V+ PP + +N+
Sbjct: 546 EGRALRVNSGPPPKNENR 599
Score = 46.2 bits (108), Expect = 9e-07
Identities = 25/77 (32%), Positives = 39/77 (50%), Gaps = 3/77 (3%)
Frame = +3
Query: 1 LAFSTTEEKLAEAFSQYGNVVKADIVLNKAKKRCKGFGYVTFAEEEEARKAQIGMNGKIL 60
LA+ L F + G VV A ++ ++ R +GFG+VTF+ +E A ++G L
Sbjct: 759 LAWGVDNAALESLFREQGRVVDAKVIYDRESGRSRGFGFVTFSSPDEVNSAIRSLDGADL 938
Query: 61 HGRVLYV---DMDPPNE 74
+GR + V D P E
Sbjct: 939 NGRAIKVSQADSKPKRE 989
>TC12531 similar to UP|Q9ASQ8 (Q9ASQ8) At1g22910/F19G10_13, partial (39%)
Length = 688
Score = 49.3 bits (116), Expect = 1e-07
Identities = 25/78 (32%), Positives = 47/78 (60%)
Frame = -1
Query: 1 LAFSTTEEKLAEAFSQYGNVVKADIVLNKAKKRCKGFGYVTFAEEEEARKAQIGMNGKIL 60
LA+ T +E + + F Q+G++++A ++ +KA R KG+G+VTF E E A +A + ++
Sbjct: 340 LAWETQKETMKKYFEQFGDILEAVVITDKATGRSKGYGFVTFREPEAAMRACVDA-APVI 164
Query: 61 HGRVLYVDMDPPNEQKNK 78
GR ++ Q++K
Sbjct: 163 DGRRANCNLASLGVQRSK 110
>TC14336 similar to UP|Q9M549 (Q9M549) Poly(A)-binding protein, partial
(85%)
Length = 1917
Score = 49.3 bits (116), Expect = 1e-07
Identities = 28/79 (35%), Positives = 41/79 (51%)
Frame = +2
Query: 4 STTEEKLAEAFSQYGNVVKADIVLNKAKKRCKGFGYVTFAEEEEARKAQIGMNGKILHGR 63
S +EKL E FS YG + V+ +G G+V F+ EEA +A + MNGK++ +
Sbjct: 704 SIADEKLKELFSSYGTITSCK-VMRDPNGISRGSGFVAFSTPEEASRALLEMNGKMVVSK 880
Query: 64 VLYVDMDPPNEQKNKIKQA 82
LYV + E + QA
Sbjct: 881 PLYVTLAQRKEDRRARLQA 937
Score = 47.0 bits (110), Expect = 6e-07
Identities = 27/89 (30%), Positives = 47/89 (52%), Gaps = 5/89 (5%)
Frame = +2
Query: 1 LAFSTTEEKLAEAFSQYGNVVKADIVLNKAKKRCKGFGYVTFAEEEEARKAQIGMNGKIL 60
L+ STT+++L F ++G + A +V+ + K FG+V F ++A +A +NGK +
Sbjct: 386 LSESTTDDELKNVFGEFGTITSA-VVMRDGDGKSKCFGFVNFENADDAARAVESLNGKKV 562
Query: 61 HGRVLYV-----DMDPPNEQKNKIKQATK 84
+ YV + E KNK +Q+ K
Sbjct: 563 DDKEWYVGKAQKKSEREQELKNKFEQSMK 649
Score = 41.2 bits (95), Expect = 3e-05
Identities = 23/81 (28%), Positives = 45/81 (55%)
Frame = +2
Query: 10 LAEAFSQYGNVVKADIVLNKAKKRCKGFGYVTFAEEEEARKAQIGMNGKILHGRVLYVDM 69
L + FS +GN++ + + + + KG+G+V F EE A+ A +NG +L+ + +YV
Sbjct: 140 LHDTFSTFGNILSCKVATDSSGQS-KGYGFVQFETEEAAQNAIEKLNGMLLNDKQVYVG- 313
Query: 70 DPPNEQKNKIKQATKNTEVDN 90
P +K + + +T + +N
Sbjct: 314 --PFLRKQERESSTDKAKFNN 370
>TC8015 similar to UP|ROC4_NICSY (P19683) 31 kDa ribonucleoprotein,
chloroplast precursor, partial (28%)
Length = 717
Score = 48.9 bits (115), Expect = 1e-07
Identities = 24/69 (34%), Positives = 40/69 (57%)
Frame = +3
Query: 1 LAFSTTEEKLAEAFSQYGNVVKADIVLNKAKKRCKGFGYVTFAEEEEARKAQIGMNGKIL 60
L +S +L E F ++GNV A IV+++ R +GFG+VT + E + A ++G+ L
Sbjct: 99 LPWSVDTARLEEIFREHGNVENARIVMDRETGRSRGFGFVTMSSEADINGAIAALDGQSL 278
Query: 61 HGRVLYVDM 69
GR + V +
Sbjct: 279 DGRTIRVSV 305
>TC14420 similar to UP|ROC3_NICSY (P19682) 28 kDa ribonucleoprotein,
chloroplast precursor (28RNP), partial (34%)
Length = 637
Score = 48.9 bits (115), Expect = 1e-07
Identities = 23/69 (33%), Positives = 41/69 (59%)
Frame = +1
Query: 1 LAFSTTEEKLAEAFSQYGNVVKADIVLNKAKKRCKGFGYVTFAEEEEARKAQIGMNGKIL 60
L + +L + FS++G VV A +V ++ R +GFG+VT ++E E A ++G+ L
Sbjct: 61 LPWDFDNSQLEQVFSEHGKVVSARVVYDRETGRSRGFGFVTMSDEAELNDAIAALDGQSL 240
Query: 61 HGRVLYVDM 69
GR + V++
Sbjct: 241 GGRTIRVNV 267
>TC16457 similar to UP|Q41124 (Q41124) Chloroplast RNA binding protein
precursor, partial (30%)
Length = 655
Score = 48.1 bits (113), Expect = 2e-07
Identities = 22/69 (31%), Positives = 39/69 (55%)
Frame = +2
Query: 1 LAFSTTEEKLAEAFSQYGNVVKADIVLNKAKKRCKGFGYVTFAEEEEARKAQIGMNGKIL 60
L+++ T E L + F +YG VV A ++ + R +G+G+V +++ E A I +N L
Sbjct: 35 LSWTVTSESLIQVFPEYGTVVGARVLYDGETGRSRGYGFVCYSKRSELETALISLNNVEL 214
Query: 61 HGRVLYVDM 69
GR + V +
Sbjct: 215 EGRAIRVSL 241
>TC19849 similar to UP|Q9AT32 (Q9AT32) Poly(A)-binding protein, partial
(30%)
Length = 603
Score = 47.4 bits (111), Expect = 4e-07
Identities = 25/70 (35%), Positives = 40/70 (56%)
Frame = +2
Query: 7 EEKLAEAFSQYGNVVKADIVLNKAKKRCKGFGYVTFAEEEEARKAQIGMNGKILHGRVLY 66
+EKL E FS++G + I + +G G+V F+ EEA +A MNGK++ G+ LY
Sbjct: 275 DEKLRELFSEFGTITSCKI-MRDPHGVSRGSGFVAFSTPEEATRALGEMNGKMVDGKPLY 451
Query: 67 VDMDPPNEQK 76
V + E++
Sbjct: 452 VALAQKKEER 481
Score = 31.2 bits (69), Expect = 0.031
Identities = 15/47 (31%), Positives = 24/47 (50%)
Frame = +2
Query: 21 VKADIVLNKAKKRCKGFGYVTFAEEEEARKAQIGMNGKILHGRVLYV 67
+ + +V+ + K FG+V F +EA KA +NGK + YV
Sbjct: 5 ITSAVVMRDVDGKSKCFGFVNFENADEAAKAVDALNGKKFDDKEWYV 145
>TC14416 similar to UP|Q8SMH8 (Q8SMH8) RNA-binding protein, partial (28%)
Length = 695
Score = 45.8 bits (107), Expect = 1e-06
Identities = 22/57 (38%), Positives = 33/57 (57%)
Frame = +1
Query: 1 LAFSTTEEKLAEAFSQYGNVVKADIVLNKAKKRCKGFGYVTFAEEEEARKAQIGMNG 57
L + KLAE F+Q G V A+++ N+ + +GFG+VT E+A KA +NG
Sbjct: 520 LPYDADGHKLAELFNQVGTVDLAEVIYNRETDQSRGFGFVTMKTVEDAEKAVDKLNG 690
>AV773507
Length = 496
Score = 45.4 bits (106), Expect = 2e-06
Identities = 21/72 (29%), Positives = 41/72 (56%)
Frame = +2
Query: 1 LAFSTTEEKLAEAFSQYGNVVKADIVLNKAKKRCKGFGYVTFAEEEEARKAQIGMNGKIL 60
L++ TE +L AF + G +++ I++ + R +GFG++TFA+ A M+G+ +
Sbjct: 32 LSWDVTERQLEHAFDRDGKILECQIMMERDTGRPRGFGFITFADRRGMEDAIKEMHGREI 211
Query: 61 HGRVLYVDMDPP 72
R++ V+ P
Sbjct: 212 GDRIISVNKAQP 247
>BP047377
Length = 564
Score = 45.1 bits (105), Expect = 2e-06
Identities = 22/68 (32%), Positives = 39/68 (57%)
Frame = +3
Query: 1 LAFSTTEEKLAEAFSQYGNVVKADIVLNKAKKRCKGFGYVTFAEEEEARKAQIGMNGKIL 60
+ + TEE+L E + G VV +V+++ + KG+G+ + +EE A A+ + G +
Sbjct: 150 IPYDATEEQLIEICQEVGPVVSFRLVIDRETGKPKGYGFCEYKDEETALSARRNLQGYEI 329
Query: 61 HGRVLYVD 68
+GR L VD
Sbjct: 330 NGRQLRVD 353
>TC11829 weakly similar to UP|NAM8_YEAST (Q00539) NAM8 protein, partial (4%)
Length = 738
Score = 43.9 bits (102), Expect = 5e-06
Identities = 20/61 (32%), Positives = 35/61 (56%)
Frame = +1
Query: 7 EEKLAEAFSQYGNVVKADIVLNKAKKRCKGFGYVTFAEEEEARKAQIGMNGKILHGRVLY 66
++ L + F Q+G +V A ++ ++ KG+G+V +A+ A A + MNG L GR +
Sbjct: 256 DDGLIQLFQQFGEIVMAKVIKDRMTGLSKGYGFVKYADITMANNAILAMNGYRLEGRTIA 435
Query: 67 V 67
V
Sbjct: 436 V 438
>TC14392 similar to UP|Q9LEB4 (Q9LEB4) RNA binding protein 45, partial (77%)
Length = 1576
Score = 43.5 bits (101), Expect = 6e-06
Identities = 23/68 (33%), Positives = 38/68 (55%), Gaps = 1/68 (1%)
Frame = +3
Query: 1 LAFSTTEEKLAEAF-SQYGNVVKADIVLNKAKKRCKGFGYVTFAEEEEARKAQIGMNGKI 59
LA T+ L E F ++Y +V A +V+++ R KG+G+V FA+E E +A M G +
Sbjct: 636 LAADVTDYHLTEVFRTRYNSVKGAKVVIDRLTSRTKGYGFVRFADESEQVRAMTEMQGVV 815
Query: 60 LHGRVLYV 67
R + +
Sbjct: 816 CSTRPMRI 839
Score = 32.3 bits (72), Expect = 0.014
Identities = 23/74 (31%), Positives = 38/74 (51%), Gaps = 2/74 (2%)
Frame = +3
Query: 4 STTEEKLAEAFSQYGNVVKADIVLNKAKKRCKGFGYVTFAEEEEARKAQIGMNGKILHGR 63
+ T++ L + F YG++V I K RC G+V FA+ A +A +NG +L G+
Sbjct: 972 NVTDDHLRQVFGLYGDLVHVKIPQGK---RC---GFVQFADRSCAEEALRVLNGTLLGGQ 1133
Query: 64 VLYVD--MDPPNEQ 75
+ + P N+Q
Sbjct: 1134 NVRLSWGRSPSNKQ 1175
>TC10587 weakly similar to GB|AAC50895.1|1518802|HSU63289 CUG-BP/hNab50
{Homo sapiens;} , partial (19%)
Length = 482
Score = 42.7 bits (99), Expect = 1e-05
Identities = 24/68 (35%), Positives = 37/68 (54%)
Frame = +1
Query: 7 EEKLAEAFSQYGNVVKADIVLNKAKKRCKGFGYVTFAEEEEARKAQIGMNGKILHGRVLY 66
++ LA AF +G V+ A + ++KA K FG+V++ E A+ A MNG L G+ L
Sbjct: 52 DQDLANAFQPFGRVISAKVFVDKATGVSKCFGFVSYDSPEAAQSAISMMNGYQLGGKKLK 231
Query: 67 VDMDPPNE 74
V N+
Sbjct: 232 VQHKRDNK 255
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.312 0.130 0.356
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,095,880
Number of Sequences: 28460
Number of extensions: 9250
Number of successful extensions: 106
Number of sequences better than 10.0: 85
Number of HSP's better than 10.0 without gapping: 97
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 105
length of query: 95
length of database: 4,897,600
effective HSP length: 71
effective length of query: 24
effective length of database: 2,876,940
effective search space: 69046560
effective search space used: 69046560
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 48 (23.1 bits)
Medicago: description of AC122162.5