Medicago
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC122162.2 - phase: 0 /pseudo
         (547 letters)

Database: LJGI 
           28,460 sequences; 14,692,800 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

AV427862                                                              170  6e-43
AV766181                                                               40  0.001
TC14118 homologue to UP|AAS18240 (AAS18240) Enolase  , complete        32  0.26
TC18275                                                                28  2.9
AV779700                                                               28  5.0

>AV427862 
          Length = 314

 Score =  170 bits (430), Expect = 6e-43
 Identities = 83/103 (80%), Positives = 88/103 (84%)
 Frame = +3

Query: 168 KQLQPVNFDELFASLYNDYTQKQEDKIWCIIHYFQRITSNMPKGVVSFERKVLPWEDDCI 227
           K LQ +NFDELF SLY+ Y+QKQE KI CIIHYFQRITSNMP+GVVSFERKVLP EDD I
Sbjct: 6   KHLQSINFDELFGSLYDYYSQKQESKIQCIIHYFQRITSNMPQGVVSFERKVLPLEDDYI 185

Query: 228 HISYPNASFWSTSVKPLCRFEVKSSGLIEDHSSETVEVDFANE 270
           HISYPNA  WSTS+ PLCRFEV SSGLIED  SE VEVDFANE
Sbjct: 186 HISYPNADVWSTSIIPLCRFEVHSSGLIEDQLSEAVEVDFANE 314


>AV766181 
          Length = 410

 Score = 40.0 bits (92), Expect = 0.001
 Identities = 18/24 (75%), Positives = 19/24 (79%)
 Frame = -3

Query: 524 GLVLDFVAGMDSWRPMEHASGVLN 547
           G  LDFV  MDSWRPMEHA+ VLN
Sbjct: 408 GCSLDFVTQMDSWRPMEHAN*VLN 337


>TC14118 homologue to UP|AAS18240 (AAS18240) Enolase  , complete
          Length = 1696

 Score = 32.0 bits (71), Expect = 0.26
 Identities = 22/76 (28%), Positives = 38/76 (49%), Gaps = 6/76 (7%)
 Frame = -3

Query: 133 LDSQEAGIVFLTQELIAALLACSFLCLFPVHDRYEKQLQPVNFDELFASLYNDYTQKQED 192
           L  QE  +  L + L    L  +F+C  P+  ++ + ++P++   LFA L  D  Q ++ 
Sbjct: 476 LSGQEHQLCRLQETLPIWHLLQAFVCTNPIR*QFHQAVEPLSCQLLFAEL--DPGQSKQG 303

Query: 193 KIWC------IIHYFQ 202
           + WC      +IH FQ
Sbjct: 302 Q*WCSHC*QPLIHPFQ 255


>TC18275 
          Length = 800

 Score = 28.5 bits (62), Expect = 2.9
 Identities = 12/34 (35%), Positives = 18/34 (52%)
 Frame = -1

Query: 151 LLACSFLCLFPVHDRYEKQLQPVNFDELFASLYN 184
           L     LC F   DRY++ L P++F++   S  N
Sbjct: 278 LFQMEILCSFNEEDRYQRDLLPLSFEDQTCSCLN 177


>AV779700 
          Length = 438

 Score = 27.7 bits (60), Expect = 5.0
 Identities = 16/52 (30%), Positives = 26/52 (49%)
 Frame = +1

Query: 164 DRYEKQLQPVNFDELFASLYNDYTQKQEDKIWCIIHYFQRITSNMPKGVVSF 215
           D+Y   L  VN  E      +   + +  KIWCI+H+ +  T+   K ++SF
Sbjct: 1   DKYNISLHIVNSLERIFIPISMGEKNKHPKIWCIVHHEK*NTA*CAKCILSF 156


  Database: LJGI
    Posted date:  Jul 30, 2004 11:16 AM
  Number of letters in database: 14,692,800
  Number of sequences in database:  28,460
  
Lambda     K      H
   0.324    0.140    0.426 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,439,173
Number of Sequences: 28460
Number of extensions: 127012
Number of successful extensions: 700
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 699
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 700
length of query: 547
length of database: 4,897,600
effective HSP length: 95
effective length of query: 452
effective length of database: 2,193,900
effective search space: 991642800
effective search space used: 991642800
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 57 (26.6 bits)


Medicago: description of AC122162.2