BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC122162.14 - phase: 0
(420 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV767121 141 2e-34
AV780221 120 5e-28
AV780705 78 2e-15
TC13572 69 7e-13
BP060613 57 6e-09
TC18742 similar to UP|Q9FZG5 (Q9FZG5) T2E6.4, partial (3%) 52 2e-07
BP078534 47 5e-06
BP041113 43 9e-05
CB828232 36 0.010
AV426437 32 0.15
BP056597 29 1.3
TC16351 similar to UP|Q84TQ7 (Q84TQ7) GIA/RGA-like gibberellin r... 29 1.7
TC13689 29 1.7
AV770881 29 1.7
BP028574 28 2.8
AV414139 27 4.8
BG662118 27 4.8
AV772606 27 4.8
AV429193 27 6.3
BP048451 27 6.3
>AV767121
Length = 525
Score = 141 bits (355), Expect = 2e-34
Identities = 68/187 (36%), Positives = 103/187 (54%)
Frame = +2
Query: 121 LHSWGNKYVSLGGRIVLLNAVINSIPIFYLSFLKMPNKVWRKIVKIQCDFLWGGARGGKK 180
L W K VS GGRI L+ +V+ ++P+F+LSF K+P V + V++ +FLWGG+ K
Sbjct: 2 LSKWKQKTVSFGGRIFLIQSVLTALPLFFLSFFKLPIGVGKSCVRLMRNFLWGGSENENK 181
Query: 181 LCWVKWRVVCQPRSKGGLGVRDIRVVNLSLLAKWRWRVLQGEEGLWKEVLIEKYGTRVCN 240
+ VKW VC+P+ GGLG++D+ N +LL K RWR L + LW+ V+ + C
Sbjct: 182 IA*VKWTDVCKPKELGGLGIKDLFTFNKALLGK*RWRYLTEPDSLWRRVIEAQPDHYSCG 361
Query: 241 LLVEDDGSWPSYISRWWKDVAFLEDEGGERWFNAEVVRKVGCGNSTSFWKDPWRGDIPFS 300
S WW D+ L E + WF++ + + VG G+ T FW + W G S
Sbjct: 362 -------------SSWWNDILSLCPEDADGWFSSGLKKLVGEGDQTKFWSEDWLGSGLLS 502
Query: 301 RKYPRLF 307
++ RL+
Sbjct: 503 ARFRRLY 523
>AV780221
Length = 461
Score = 120 bits (300), Expect = 5e-28
Identities = 58/163 (35%), Positives = 92/163 (55%), Gaps = 2/163 (1%)
Frame = +3
Query: 135 IVLLNAVINSIPIFYLSFLKMPNKVWRKIVKIQCDFLWGG-ARGGKKLCWVKWRVVCQPR 193
+ L+ +V+ ++P+F+L F K+P + V D +G GGKK+ WVKW VC+P+
Sbjct: 3 LCLIRSVLIALPLFHLPFFKLPTGGFYTNVNN**DPFFGREVEGGKKIAWVKWSTVCRPK 182
Query: 194 SKGGLGVRDIRVVNLSLLAKWRWRVLQGEEGLWKEVLIEKYGTRVCNLLVEDDGSWPSYI 253
+GGLGV+++ + N +LL KWRWR+L+ +GLW +VL+ KY + P
Sbjct: 183 DEGGLGVQNLGLFNKALLGKWRWRMLKERDGLWYKVLLIKYKNAI-----------PQSA 329
Query: 254 SRWWKDV-AFLEDEGGERWFNAEVVRKVGCGNSTSFWKDPWRG 295
S WW D+ + ++GG W + R++G G FW + W G
Sbjct: 330 SSWWNDLHSTCFEDGGGGWMQRGLCRRIGEGTEVKFWNENWLG 458
>AV780705
Length = 524
Score = 78.2 bits (191), Expect = 2e-15
Identities = 49/162 (30%), Positives = 74/162 (45%), Gaps = 3/162 (1%)
Frame = -2
Query: 92 PFKYLGLPV--GANSFKLVTWEPLLEQLSRKLHSWGNKYVSLGGRIVLLNAVINSIPIFY 149
P YLGLP G N + W + E++ KL W ++ G+ VL+ A+I +IP +
Sbjct: 493 PGHYLGLPAIWGRNKSHSLVW--IEEKVKEKLEGWKETLLNQAGKEVLIKAIIQAIPSYA 320
Query: 150 LSFLKMPNKVWRKIVKIQCDFLWGG-ARGGKKLCWVKWRVVCQPRSKGGLGVRDIRVVNL 208
++ + P + + DF W G + + KGG+G RD+R NL
Sbjct: 319 MTIVHFPKTFCNSLYALVADFWWKSQGEGAVEYTGAVGTK*PLSKEKGGVGFRDLRTQNL 140
Query: 209 SLLAKWRWRVLQGEEGLWKEVLIEKYGTRVCNLLVEDDGSWP 250
+ LA+ WRVL E LW V+ Y L+ DG+ P
Sbjct: 139 AFLARQAWRVLTNPEALWVRVMKSLYFPTPALCLLLWDGTHP 14
>TC13572
Length = 534
Score = 68.6 bits (166), Expect(2) = 7e-13
Identities = 47/143 (32%), Positives = 63/143 (43%), Gaps = 2/143 (1%)
Frame = -3
Query: 226 WKEVLIEKYGTRVCNLLVEDDGSWPSYISRWWKDVAFLEDEGGER-WFNAEVVRKVGCGN 284
W V++ KYG C + IS WW+DV GGE WF V + + GN
Sbjct: 478 WCRVVLAKYGDGGC-----------TNISSWWRDVQTAG--GGENGWFEEGVHKVLRSGN 338
Query: 285 STSFWKDPWRGDIPFSRKYPRLFAISNNKETLVEECRQMNGVGGGWIFEWHRPLFVWEEE 344
T FW + W G KY RL+ +S K ++EC + W F W RPL E +
Sbjct: 337 HTRFWLENWTGVGILREKYYRLYNLSKLKWASIDECGGWSQGVWNWDFRWRRPLAGRELD 158
Query: 345 LLISLKEDL-EGHRWVNDPDRWV 366
L +L D+ PD+WV
Sbjct: 157 WLQALSLDIVRVPLLAGVPDKWV 89
Score = 21.6 bits (44), Expect(2) = 7e-13
Identities = 7/12 (58%), Positives = 9/12 (74%)
Frame = -1
Query: 207 NLSLLAKWRWRV 218
N +LL KW WR+
Sbjct: 534 NKALLGKWLWRL 499
>BP060613
Length = 378
Score = 57.0 bits (136), Expect = 6e-09
Identities = 22/64 (34%), Positives = 39/64 (60%)
Frame = +1
Query: 171 LWGGARGGKKLCWVKWRVVCQPRSKGGLGVRDIRVVNLSLLAKWRWRVLQGEEGLWKEVL 230
+W ++G + + W KW ++ + + +GGLG +D + N +LLAK WR+L + LW ++L
Sbjct: 43 VWWSSKGTRGVHWRKWDLLTELKDEGGLGFKDFEIQNQALLAKQAWRILHNPDALWVQIL 222
Query: 231 IEKY 234
Y
Sbjct: 223 KALY 234
>TC18742 similar to UP|Q9FZG5 (Q9FZG5) T2E6.4, partial (3%)
Length = 912
Score = 52.0 bits (123), Expect = 2e-07
Identities = 34/124 (27%), Positives = 61/124 (48%)
Frame = -3
Query: 22 ISHLQYADDTLCIGEASVDNLWTLKALLRGFEMASGLKVNFYKSCLMGINVPSEFMTMAC 81
ISHL +AD+ + ++++ + +L F+ SGL N KS + I V
Sbjct: 373 ISHLCFADNLMVFSNGYLESIAIINNVLHIFQHLSGLTPNPAKSEVF-ILVKETTKNQIT 197
Query: 82 DFLNCSEGVVPFKYLGLPVGANSFKLVTWEPLLEQLSRKLHSWGNKYVSLGGRIVLLNAV 141
+ L EG +P + LG+P K + L+++ KL W + +S GR+ L+N++
Sbjct: 196 NMLGYKEGKLPVR*LGVPQLTTKLKASDCKVLVDRKPSKLQHWTGRSLSYAGRLQLINSI 17
Query: 142 INSI 145
+ S+
Sbjct: 16 LFSM 5
>BP078534
Length = 410
Score = 47.4 bits (111), Expect = 5e-06
Identities = 21/63 (33%), Positives = 30/63 (47%)
Frame = -3
Query: 287 SFWKDPWRGDIPFSRKYPRLFAISNNKETLVEECRQMNGVGGGWIFEWHRPLFVWEEELL 346
+ W DPW + +PRLF + NK LV + +G W + R F WEEE+
Sbjct: 222 NLWLDPWIRERALCDMFPRLFTLCENKGALVADVGSYHGGSWHWNLSFRREFFQWEEEIH 43
Query: 347 ISL 349
+ L
Sbjct: 42 LQL 34
>BP041113
Length = 503
Score = 43.1 bits (100), Expect = 9e-05
Identities = 37/132 (28%), Positives = 51/132 (38%), Gaps = 10/132 (7%)
Frame = +1
Query: 288 FWKDPWRGDIPFSRKYPRLFAISNNKETLVEECRQMNGVGGGWIFEWHRPLFVWEEELLI 347
FW + W G +Y RLF +S K + EC N W W R L E L
Sbjct: 1 FWTEDWIGMGTLRDRYYRLFNLSKEKRCCILECGGWNHGVWTWKLSWRRALVGRELGWLE 180
Query: 348 SLKEDLEG---HRWVNDPDRW-------VKSCYGKLERLLGGEVEWSLEELRVLESIWNS 397
+ +DL G V D W V S Y L+ + + + +IW +
Sbjct: 181 LMMKDLSGVCLSEGVEDKWLWLPGGTYTVNSAYSFLQAPTLADTD------PIFATIWRT 342
Query: 398 KAPLKVIAFSWK 409
AP V AF+W+
Sbjct: 343 VAP-SVKAFAWR 375
>CB828232
Length = 532
Score = 36.2 bits (82), Expect = 0.010
Identities = 19/43 (44%), Positives = 27/43 (62%), Gaps = 1/43 (2%)
Frame = +1
Query: 132 GGRIVLLNAVINSIPIFYLSFLKMPNKVWRKIVK-IQCDFLWG 173
G R+ V++S+P+F+LSF K+ K K+ K IQ FLWG
Sbjct: 409 GARVCCFKMVLSSLPLFFLSFFKI--KCVAKVCKQIQSRFLWG 531
>AV426437
Length = 303
Score = 32.3 bits (72), Expect = 0.15
Identities = 9/24 (37%), Positives = 17/24 (70%)
Frame = +1
Query: 179 KKLCWVKWRVVCQPRSKGGLGVRD 202
+K+ W+ W +C+P+ GLGV++
Sbjct: 223 RKIAWINWETICKPKWLRGLGVKN 294
>BP056597
Length = 414
Score = 29.3 bits (64), Expect = 1.3
Identities = 25/122 (20%), Positives = 46/122 (37%), Gaps = 14/122 (11%)
Frame = +3
Query: 303 YPRLFAISNNKETLVEECRQMNGVGGGWIFEWHRPLFVWEEELLISLKEDLEGHRWVNDP 362
+ LF ISN + + E W F W P+ + L +K+ + +N
Sbjct: 9 FDSLFLISNQQNCKIVEMGHWLENKWVWDFSWKNPISGEDVLKLGVMKQIVSSFSLINGK 188
Query: 363 -DRWVKSCYGKLERLLGGEVEWSLEE-------------LRVLESIWNSKAPLKVIAFSW 408
D WV +L G+ ++S++ + V +W + AP +A W
Sbjct: 189 KDTWVW--------ILEGDGQYSVKSAYDLLSGLDTTIGISVFSKLWKACAPSNAVALGW 344
Query: 409 KL 410
++
Sbjct: 345 RV 350
>TC16351 similar to UP|Q84TQ7 (Q84TQ7) GIA/RGA-like gibberellin response
modulator, partial (11%)
Length = 756
Score = 28.9 bits (63), Expect = 1.7
Identities = 14/36 (38%), Positives = 21/36 (57%)
Frame = -3
Query: 204 RVVNLSLLAKWRWRVLQGEEGLWKEVLIEKYGTRVC 239
RVV+L + + RW + QG G W+ V + +YG C
Sbjct: 619 RVVDLVIFQERRW-IKQGGGGGWRRVELGEYGLNPC 515
>TC13689
Length = 524
Score = 28.9 bits (63), Expect = 1.7
Identities = 9/13 (69%), Positives = 11/13 (84%)
Frame = -1
Query: 182 CWVKWRVVCQPRS 194
CW+KWRV QPR+
Sbjct: 239 CWIKWRVAQQPRN 201
>AV770881
Length = 403
Score = 28.9 bits (63), Expect = 1.7
Identities = 12/36 (33%), Positives = 19/36 (52%)
Frame = -2
Query: 68 MGINVPSEFMTMACDFLNCSEGVVPFKYLGLPVGAN 103
MG+ + S++ T C NC +VPF + + V N
Sbjct: 108 MGLKISSDWETYCCQC*NCHHNLVPFDIIAVRVIQN 1
>BP028574
Length = 454
Score = 28.1 bits (61), Expect = 2.8
Identities = 18/55 (32%), Positives = 28/55 (50%), Gaps = 2/55 (3%)
Frame = +3
Query: 358 WVNDPDR--WVKSCYGKLERLLGGEVEWSLEELRVLESIWNSKAPLKVIAFSWKL 410
W+ D VKS Y +L GE++ +E+ +W SKAP IA +W++
Sbjct: 273 WIKDGSGSFTVKSAYEELH----GEIQ-EIED-NFFRKLWQSKAPSNSIALAWRV 419
>AV414139
Length = 309
Score = 27.3 bits (59), Expect = 4.8
Identities = 25/93 (26%), Positives = 34/93 (35%), Gaps = 12/93 (12%)
Frame = -2
Query: 330 WIFEWHRPLFVWEEELLISLKEDLEG---HRWVNDPDRW---------VKSCYGKLERLL 377
W F W R L V E+ L + L G + D +W V+S Y L +
Sbjct: 269 WEFRWRRELSVREQRQEADLIQALAGVCLQQGTEDRWKWTLDTEDIYTVRSAY*LLLDPI 90
Query: 378 GGEVEWSLEELRVLESIWNSKAPLKVIAFSWKL 410
+ V E +W AP AF W+L
Sbjct: 89 ADS------DSEVYEKVWVGIAPSNANAFVWRL 9
>BG662118
Length = 421
Score = 27.3 bits (59), Expect = 4.8
Identities = 27/78 (34%), Positives = 35/78 (44%), Gaps = 9/78 (11%)
Frame = -2
Query: 317 VEECRQMNGVGGGWIFEWHRP--LFVWEEELLISLKEDLEGHR--WVNDPDRWVKSCY-- 370
VE QM W+ RP LF + L++ L +R W+N P +W CY
Sbjct: 318 VEHQHQMRQGC*DWL---RRPNQLFACGDPLILISPHPL*HYRCEWLNPPSQWQ*CCYPE 148
Query: 371 -GKLERLLGGEVE--WSL 385
GKLER + V WSL
Sbjct: 147 DGKLER*MQEVVA*MWSL 94
>AV772606
Length = 487
Score = 27.3 bits (59), Expect = 4.8
Identities = 16/54 (29%), Positives = 25/54 (45%), Gaps = 3/54 (5%)
Frame = +1
Query: 32 LCIGEASVDNLWTLKALLRGFEMASGLKVNFYKSC---LMGINVPSEFMTMACD 82
+C+GEA+ N+ L R + N Y C + GIN ++F T+ D
Sbjct: 1 ICVGEANTINILRLNICRRQMQYLCRCAYNNYHECCITVTGINFTTKFDTII*D 162
>AV429193
Length = 295
Score = 26.9 bits (58), Expect = 6.3
Identities = 12/33 (36%), Positives = 20/33 (60%)
Frame = +1
Query: 197 GLGVRDIRVVNLSLLAKWRWRVLQGEEGLWKEV 229
GL + + + +SL A+ WR+L G + LW+ V
Sbjct: 175 GLMINAVVLAFMSLAAEPLWRILGGVKNLWRIV 273
>BP048451
Length = 400
Score = 26.9 bits (58), Expect = 6.3
Identities = 9/12 (75%), Positives = 10/12 (83%)
Frame = +3
Query: 353 LEGHRWVNDPDR 364
L+GH W NDPDR
Sbjct: 228 LQGHMWSNDPDR 263
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.323 0.141 0.472
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,203,090
Number of Sequences: 28460
Number of extensions: 184465
Number of successful extensions: 931
Number of sequences better than 10.0: 48
Number of HSP's better than 10.0 without gapping: 910
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 923
length of query: 420
length of database: 4,897,600
effective HSP length: 93
effective length of query: 327
effective length of database: 2,250,820
effective search space: 736018140
effective search space used: 736018140
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 56 (26.2 bits)
Medicago: description of AC122162.14