BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC122162.12 - phase: 0
(400 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP028499 75 2e-14
AV422434 59 3e-11
BP069765 55 2e-08
BI419994 33 0.064
BP038938 32 0.19
BP052653 32 0.24
BP076687 31 0.42
TC18734 similar to UP|Q8LFM3 (Q8LFM3) Pectinesterase-like protei... 30 0.93
AV414925 30 0.93
BP043571 29 1.2
AI967849 29 1.6
TC10231 similar to UP|O23110 (O23110) AP2 domain containing prot... 28 2.1
TC13688 similar to UP|O22161 (O22161) At2g44900 protein, partial... 28 2.1
AV776145 28 2.7
TC11167 weakly similar to UP|Q9FLC7 (Q9FLC7) Similarity to chalc... 28 2.7
BP063694 28 2.7
AV770380 28 3.5
BP035281 28 3.5
AV427033 28 3.5
CB826914 28 3.5
>BP028499
Length = 491
Score = 75.1 bits (183), Expect = 2e-14
Identities = 54/148 (36%), Positives = 75/148 (50%), Gaps = 8/148 (5%)
Frame = +2
Query: 3 YFHTAVATSSMASVDWSELPKDIIFLISQRLDVELDLIRFRSVCSTWR----SSPIPNDH 58
+ H S A DWS+LP +++ LISQRL+ L L+RFRSVCSTWR SS IP H
Sbjct: 74 HHHHTTNPSMAAPPDWSQLPAELLHLISQRLNSPLYLLRFRSVCSTWRHSSASSFIPIHH 253
Query: 59 NILPFQFPLLKYVPTPDSISNNNEIIDNIENTSTSFGHLSKRSFFLVKPPQEQQQQETLI 118
PF FP L D+ + ++SF L+KR+ L+ PP Q
Sbjct: 254 --FPFNFPPLS---------------DHDHHDASSFS-LTKRTVVLITPPPPPHHQ---- 367
Query: 119 RRRPWLIRITQN----SSGKTQLLKPPF 142
+ PWL++I ++ +T+L P F
Sbjct: 368 TQTPWLVKIGEDLCDGDRTRTRLWHPLF 451
>AV422434
Length = 422
Score = 58.5 bits (140), Expect(2) = 3e-11
Identities = 26/38 (68%), Positives = 32/38 (83%)
Frame = +3
Query: 15 SVDWSELPKDIIFLISQRLDVELDLIRFRSVCSTWRSS 52
+VDWS+LP +++ LISQRLD L L+RFRSVCSTWR S
Sbjct: 51 AVDWSQLPTELLHLISQRLDSPLYLLRFRSVCSTWRRS 164
Score = 25.8 bits (55), Expect(2) = 3e-11
Identities = 21/64 (32%), Positives = 35/64 (53%)
Frame = +1
Query: 88 ENTSTSFGHLSKRSFFLVKPPQEQQQQETLIRRRPWLIRITQNSSGKTQLLKPPFLSLTS 147
++T+++F LS+R+ FL+ PP Q + P L +I+Q+ +G T+ L P S
Sbjct: 211 DDTASTF-FLSQRTVFLIIPPPLQPTEPL----TPLLFQISQD-NG*TRRLYDPLSSHRD 372
Query: 148 TAFS 151
FS
Sbjct: 373 PPFS 384
>BP069765
Length = 417
Score = 55.1 bits (131), Expect = 2e-08
Identities = 32/90 (35%), Positives = 49/90 (53%), Gaps = 6/90 (6%)
Frame = -2
Query: 315 KKWVKLKKLGGRVLFVGSGCSFSASGWDLCLPKGNCVIFIDTSVLSSDNMAGGNRVFHLD 374
+ WV ++ + RVLF+G C+FS S DL + +GN V+F D D++ G VF LD
Sbjct: 416 ESWVHVRNVRDRVLFIGDDCAFSVSASDLGVDEGNYVVFRD------DDLKSGIGVFPLD 255
Query: 375 -----RGQLSHVSEYPECENLFL-PPEWIL 398
+ H+ +YP +LF PP+W +
Sbjct: 254 DVGWYGDGILHLLDYPGFSSLFRPPPDWAM 165
>BI419994
Length = 549
Score = 33.5 bits (75), Expect = 0.064
Identities = 18/46 (39%), Positives = 28/46 (60%)
Frame = +2
Query: 5 HTAVATSSMASVDWSELPKDIIFLISQRLDVELDLIRFRSVCSTWR 50
H +T S++ +S LP ++I I RL V L+RF++VC +WR
Sbjct: 5 HPQTSTESLSL--FSHLPDELILQILLRLPVR-SLLRFKTVCKSWR 133
>BP038938
Length = 583
Score = 32.0 bits (71), Expect = 0.19
Identities = 15/46 (32%), Positives = 25/46 (53%)
Frame = +3
Query: 6 TAVATSSMASVDWSELPKDIIFLISQRLDVELDLIRFRSVCSTWRS 51
T V ++ WS+LP +++ L+ RL ++ D +R VC W S
Sbjct: 99 TEVKNENLELQSWSDLPAELLELVMTRLALD-DNVRASVVCKRWHS 233
>BP052653
Length = 552
Score = 31.6 bits (70), Expect = 0.24
Identities = 17/33 (51%), Positives = 21/33 (63%)
Frame = +1
Query: 19 SELPKDIIFLISQRLDVELDLIRFRSVCSTWRS 51
S LP D+I I RL V L+RF+SVC +W S
Sbjct: 181 SILPHDLIVQILLRLPVR-SLLRFKSVCKSWLS 276
>BP076687
Length = 389
Score = 30.8 bits (68), Expect = 0.42
Identities = 16/33 (48%), Positives = 22/33 (66%)
Frame = -3
Query: 19 SELPKDIIFLISQRLDVELDLIRFRSVCSTWRS 51
S LP+++I I RL V L+RF+SVC +W S
Sbjct: 387 SHLPEELIPQILLRLPVR-SLLRFKSVCKSWLS 292
>TC18734 similar to UP|Q8LFM3 (Q8LFM3) Pectinesterase-like protein, partial
(47%)
Length = 929
Score = 29.6 bits (65), Expect = 0.93
Identities = 21/81 (25%), Positives = 41/81 (49%), Gaps = 3/81 (3%)
Frame = -1
Query: 12 SMASVDWSELPKDIIFLISQRLDVELDLI---RFRSVCSTWRSSPIPNDHNILPFQFPLL 68
SM +++ S+ + + L ++ + +LI + SV WR+ + + + ILP FPLL
Sbjct: 443 SMKNIESSKPSRSFMHLS*WKVCISANLILGLKLVSVI*VWRNGTVSS*NPILPRIFPLL 264
Query: 69 KYVPTPDSISNNNEIIDNIEN 89
+P +++D +EN
Sbjct: 263 NAIPCEQEWL--IQLVDEVEN 207
>AV414925
Length = 414
Score = 29.6 bits (65), Expect = 0.93
Identities = 15/32 (46%), Positives = 21/32 (64%)
Frame = +1
Query: 20 ELPKDIIFLISQRLDVELDLIRFRSVCSTWRS 51
E+P ++I I RL V L+RFRS+ +WRS
Sbjct: 94 EIPVEVIADILSRLPVT-SLLRFRSISKSWRS 186
>BP043571
Length = 438
Score = 29.3 bits (64), Expect = 1.2
Identities = 12/40 (30%), Positives = 20/40 (50%)
Frame = +1
Query: 289 GELLLLDIHQTFFQFSIKFFKLDEMEKKWVKLKKLGGRVL 328
G +L +++ F +K FK+ + W KLGG+ L
Sbjct: 91 GRILTCTLNKKIFTVEVKNFKMSKTRNSWKDHSKLGGKSL 210
>AI967849
Length = 263
Score = 28.9 bits (63), Expect = 1.6
Identities = 17/33 (51%), Positives = 18/33 (54%)
Frame = +1
Query: 209 LSYCSSKRVLFHDRDKRWTPVSNLSTAHGDICL 241
+S CSS R F DR W V NLS HG I L
Sbjct: 133 MSMCSSLRHCFEDRGCSWRGVRNLS--HGTINL 225
>TC10231 similar to UP|O23110 (O23110) AP2 domain containing protein RAP2.8
(Fragment), partial (22%)
Length = 707
Score = 28.5 bits (62), Expect = 2.1
Identities = 21/66 (31%), Positives = 33/66 (49%)
Frame = -1
Query: 83 IIDNIENTSTSFGHLSKRSFFLVKPPQEQQQQETLIRRRPWLIRITQNSSGKTQLLKPPF 142
++ + EN S SF F ++PP E + L + + T +++G T + KPP
Sbjct: 446 LLHSKENRSISF-------LFPLQPPSEPGSFKRLTPKSRTIC--TGSTTGPTGINKPPV 294
Query: 143 LSLTST 148
L LTST
Sbjct: 293 LMLTST 276
>TC13688 similar to UP|O22161 (O22161) At2g44900 protein, partial (7%)
Length = 621
Score = 28.5 bits (62), Expect = 2.1
Identities = 15/40 (37%), Positives = 18/40 (44%)
Frame = -3
Query: 12 SMASVDWSELPKDIIFLISQRLDVELDLIRFRSVCSTWRS 51
S V+W LP D + + L D F S C TWRS
Sbjct: 181 SQGIVEWRGLPDDTVIQLLSCLSYR-DRASFSSSCRTWRS 65
>AV776145
Length = 275
Score = 28.1 bits (61), Expect = 2.7
Identities = 12/43 (27%), Positives = 25/43 (57%)
Frame = -3
Query: 65 FPLLKYVPTPDSISNNNEIIDNIENTSTSFGHLSKRSFFLVKP 107
FP+L+Y P S+N+ +I+ + +T+ F +F+++P
Sbjct: 258 FPILRYHPATFRESDNSHLINPLPHTTFFFCRYIYPLYFIIEP 130
>TC11167 weakly similar to UP|Q9FLC7 (Q9FLC7) Similarity to
chalcone-flavonone isomerase, partial (69%)
Length = 825
Score = 28.1 bits (61), Expect = 2.7
Identities = 23/67 (34%), Positives = 33/67 (48%), Gaps = 3/67 (4%)
Frame = -1
Query: 91 STSFGHLSKRSFFLVKPPQEQQQQ--ETLIRRRPWLIRITQNS-SGKTQLLKPPFLSLTS 147
S GH ++ L+ PP+ QQ Q E L+ L+ + ++ +GKTQ L P L L
Sbjct: 597 SLPHGHSLLQTGCLLFPPKTQQSQQLENLLGNEM*LLNFS*ST*TGKTQQLVPKQLLLHP 418
Query: 148 TAFSHLP 154
HLP
Sbjct: 417 HICHHLP 397
>BP063694
Length = 511
Score = 28.1 bits (61), Expect = 2.7
Identities = 15/45 (33%), Positives = 24/45 (53%)
Frame = -2
Query: 188 LYPQKILAVTCPEKKPLVLGTLSYCSSKRVLFHDRDKRWTPVSNL 232
L+ L V+ KP+ + SKR++FHD +KR + V +L
Sbjct: 312 LHKHGCLDVSSILPKPICCTSCQMAKSKRLVFHDNNKRASAVLDL 178
>AV770380
Length = 526
Score = 27.7 bits (60), Expect = 3.5
Identities = 14/35 (40%), Positives = 24/35 (68%)
Frame = -3
Query: 191 QKILAVTCPEKKPLVLGTLSYCSSKRVLFHDRDKR 225
++ILA +KP+ + + SS+R+LFHD+DK+
Sbjct: 143 KRILACETQAEKPIKCFSFAP-SSQRLLFHDKDKK 42
>BP035281
Length = 498
Score = 27.7 bits (60), Expect = 3.5
Identities = 13/30 (43%), Positives = 19/30 (63%)
Frame = +2
Query: 22 PKDIIFLISQRLDVELDLIRFRSVCSTWRS 51
P +++ I L V L LIRF+ VC +W+S
Sbjct: 125 PSELVIEILSWLPV-LSLIRFKCVCKSWKS 211
>AV427033
Length = 420
Score = 27.7 bits (60), Expect = 3.5
Identities = 14/31 (45%), Positives = 19/31 (61%)
Frame = +1
Query: 21 LPKDIIFLISQRLDVELDLIRFRSVCSTWRS 51
LP D++ I RL V L++FR VC +W S
Sbjct: 124 LPFDLVVEILCRLPVN-SLLQFRCVCKSWNS 213
>CB826914
Length = 575
Score = 27.7 bits (60), Expect = 3.5
Identities = 12/32 (37%), Positives = 21/32 (65%)
Frame = +1
Query: 20 ELPKDIIFLISQRLDVELDLIRFRSVCSTWRS 51
++ +D+I I L V+ ++RF++VC WRS
Sbjct: 163 DMDRDLIIEILLMLPVK-SIVRFKAVCKLWRS 255
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.322 0.138 0.422
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,245,088
Number of Sequences: 28460
Number of extensions: 129368
Number of successful extensions: 675
Number of sequences better than 10.0: 50
Number of HSP's better than 10.0 without gapping: 673
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 674
length of query: 400
length of database: 4,897,600
effective HSP length: 92
effective length of query: 308
effective length of database: 2,279,280
effective search space: 702018240
effective search space used: 702018240
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 56 (26.2 bits)
Medicago: description of AC122162.12