BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC122160.6 + phase: 0
(466 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC16020 similar to UP|Q9SLX0 (Q9SLX0) Importin alpha 1b, partial... 426 e-120
TC18614 similar to UP|Q94KA9 (Q94KA9) Importin alpha 2, partial ... 113 6e-26
TC13040 weakly similar to UP|Q94KA9 (Q94KA9) Importin alpha 2, p... 75 2e-14
AV409921 70 6e-13
AV766757 35 0.035
AV420018 31 0.38
TC18720 weakly similar to UP|SCWA_YEAST (Q04951) Probable family... 30 0.85
AV410889 28 3.2
CN825562 28 3.2
AV422643 27 7.2
>TC16020 similar to UP|Q9SLX0 (Q9SLX0) Importin alpha 1b, partial (55%)
Length = 951
Score = 426 bits (1095), Expect = e-120
Identities = 219/330 (66%), Positives = 249/330 (75%), Gaps = 1/330 (0%)
Frame = +3
Query: 132 LNEQEGLYRLRNAVWTLSNFCRGKPQPALEQVRSALPALKCLVFSKDEVVLTEACWALSY 191
+ + L LRNA WTLSNFCRGKPQP +QV+ ALPAL L+ S DE VLT+ACWALSY
Sbjct: 3 IRHEAKLSMLRNATWTLSNFCRGKPQPPFDQVKPALPALTSLIHSNDEEVLTDACWALSY 182
Query: 192 LSDGTSDNIQAVIEAGVCGRLVQILLEPSLSALFPALRTVGNIVSGDDMQTQAIVNHGLL 251
LSDGT+D IQAVIEAGVC RLV++LL PS S L PALRTVGNIV+GDDMQTQ I+NH L
Sbjct: 183 LSDGTNDKIQAVIEAGVCPRLVELLLHPSPSVLIPALRTVGNIVTGDDMQTQVIINHQAL 362
Query: 252 PCLLSLL-THPKKSIKKEACRTLSNITAGNREQIQAVIEAGLIAPLVNLLQNAEFDITKE 310
PCLL+LL ++ KKSIKKEAC T+SNITAGN++QIQ+V+EA +I PLVNLLQNAEFDI KE
Sbjct: 363 PCLLNLLASNYKKSIKKEACWTISNITAGNKQQIQSVVEANIIGPLVNLLQNAEFDIKKE 542
Query: 311 VAQALTNVTSGGTHEHIKYLVSQGCIKPLCDLLVCPDPRIVTVCLEGLENFLKVGEAEKS 370
A A++N TSGG HE IKYLVSQGCIKPLCDLL+CPDPRIVTVCLEGLEN LKVGEA+K+
Sbjct: 543 AAWAISNATSGGAHEQIKYLVSQGCIKPLCDLLICPDPRIVTVCLEGLENILKVGEADKN 722
Query: 371 ISNTGDVNLYAQTIKDVEGLEKGLEKFLKVGEAEKRFGNTGDVNLYAQMIEDVKGLEKIE 430
N+GDVNLYAQ I D EG LEKIE
Sbjct: 723 TGNSGDVNLYAQMIDDAEG------------------------------------LEKIE 794
Query: 431 NLDSHEDREIYEKAAKILERYWLEDEDETL 460
NL SH++ EIYEKA KILE YWLE+EDET+
Sbjct: 795 NLQSHDNTEIYEKAVKILETYWLEEEDETM 884
Score = 73.2 bits (178), Expect = 9e-14
Identities = 68/264 (25%), Positives = 117/264 (43%), Gaps = 18/264 (6%)
Frame = +3
Query: 28 KPPIDDVIQSGVVPRFVQFLDKGDFPQLQLEAAWALTNIAAGTSENTKVVVDHGAVPMFV 87
+PP D V +P + D ++ +A WAL+ ++ GT++ + V++ G P V
Sbjct: 78 QPPFDQV--KPALPALTSLIHSND-EEVLTDACWALSYLSDGTNDKIQAVIEAGVCPRLV 248
Query: 88 KLLSSPCDDVRGQAAWALGNIAGDSPRGRDLVLSHGALILLLSQLNEQEGLYRLRNAVWT 147
+LL P V A +GNI ++++H AL LL+ L + A WT
Sbjct: 249 ELLLHPSPSVLIPALRTVGNIVTGDDMQTQVIINHQALPCLLNLLASNYKKSIKKEACWT 428
Query: 148 LSNFCRGKPQPALEQVRSALPA-----LKCLVFSKDEVVLTEACWALS-YLSDGTSDNIQ 201
+SN G Q Q++S + A L L+ + + + EA WA+S S G + I+
Sbjct: 429 ISNITAGNKQ----QIQSVVEANIIGPLVNLLQNAEFDIKKEAAWAISNATSGGAHEQIK 596
Query: 202 AVIEAGVCGRLVQILLEPSLSALFPALRTVGNIV-----------SGD-DMQTQAIVNHG 249
++ G L +L+ P + L + NI+ SGD ++ Q I +
Sbjct: 597 YLVSQGCIKPLCDLLICPDPRIVTVCLEGLENILKVGEADKNTGNSGDVNLYAQMIDDAE 776
Query: 250 LLPCLLSLLTHPKKSIKKEACRTL 273
L + +L +H I ++A + L
Sbjct: 777 GLEKIENLQSHDNTEIYEKAVKIL 848
>TC18614 similar to UP|Q94KA9 (Q94KA9) Importin alpha 2, partial (27%)
Length = 538
Score = 113 bits (283), Expect = 6e-26
Identities = 55/74 (74%), Positives = 66/74 (88%), Gaps = 1/74 (1%)
Frame = +3
Query: 1 MVADIWSDDNSQQLEATTEFRKRLSVDK-PPIDDVIQSGVVPRFVQFLDKGDFPQLQLEA 59
MVA +WSDDNS QLE+TT+FRK LS+++ PPI++VIQSGVV RFV+FL + DFPQLQ EA
Sbjct: 315 MVAGVWSDDNSMQLESTTQFRKLLSIERTPPIEEVIQSGVVSRFVEFLMREDFPQLQFEA 494
Query: 60 AWALTNIAAGTSEN 73
AWALTNIA+GTSEN
Sbjct: 495 AWALTNIASGTSEN 536
>TC13040 weakly similar to UP|Q94KA9 (Q94KA9) Importin alpha 2, partial
(14%)
Length = 503
Score = 75.5 bits (184), Expect = 2e-14
Identities = 35/49 (71%), Positives = 39/49 (79%)
Frame = +1
Query: 414 NLYAQMIEDVKGLEKIENLDSHEDREIYEKAAKILERYWLEDEDETLTS 462
NLYAQMIED GLEKI+NL +H++ EIYEK KILE YW ED DETL S
Sbjct: 1 NLYAQMIEDADGLEKIDNLQNHDNNEIYEKVVKILETYWSEDVDETLPS 147
>AV409921
Length = 414
Score = 70.5 bits (171), Expect = 6e-13
Identities = 36/56 (64%), Positives = 46/56 (81%), Gaps = 1/56 (1%)
Frame = +1
Query: 1 MVADIWSDDNSQQLEATTEFRKRLSVDK-PPIDDVIQSGVVPRFVQFLDKGDFPQL 55
MV + S DN+ QLEAT +FRK LS+++ PPI++VIQSGVVPRFV+FL + DFPQL
Sbjct: 247 MVNGVCSVDNNLQLEATAQFRKLLSMERNPPIEEVIQSGVVPRFVEFLVREDFPQL 414
>AV766757
Length = 601
Score = 34.7 bits (78), Expect = 0.035
Identities = 25/75 (33%), Positives = 36/75 (47%)
Frame = +2
Query: 34 VIQSGVVPRFVQFLDKGDFPQLQLEAAWALTNIAAGTSENTKVVVDHGAVPMFVKLLSSP 93
++ SG VP V L KG + + AA L +++ EN + GA+P V LLS
Sbjct: 95 IVSSGAVPGIVHVLRKGSM-EARENAAATLFSLSV-VDENKVTIGSSGAIPPLVTLLSEG 268
Query: 94 CDDVRGQAAWALGNI 108
+ AA AL N+
Sbjct: 269 TQRGKKDAATALFNL 313
Score = 29.3 bits (64), Expect = 1.4
Identities = 47/198 (23%), Positives = 85/198 (42%)
Frame = +2
Query: 87 VKLLSSPCDDVRGQAAWALGNIAGDSPRGRDLVLSHGALILLLSQLNEQEGLYRLRNAVW 146
V LLS P + A AL N++ + ++S GA+ ++ L + R NA
Sbjct: 2 VGLLSVPDSRTQEHAVTALLNLS-IYENNKGCIVSSGAVPGIVHVLRKGSMEAR-ENAAA 175
Query: 147 TLSNFCRGKPQPALEQVRSALPALKCLVFSKDEVVLTEACWALSYLSDGTSDNIQAVIEA 206
TL + A+P L L+ + +A AL L + +AV A
Sbjct: 176 TLFSLSVVDENKVTIGSSGAIPPLVTLLSEGTQRGKKDAATALFNLCIYQGNKGKAV-RA 352
Query: 207 GVCGRLVQILLEPSLSALFPALRTVGNIVSGDDMQTQAIVNHGLLPCLLSLLTHPKKSIK 266
GV L+++L EPS + AL + + S D + AI +P L+ + + K
Sbjct: 353 GVIPTLMKLLTEPSGGMVDEALAILAILSSHPDGKA-AIGAADAVPILVEFIGNGSPRNK 529
Query: 267 KEACRTLSNITAGNREQI 284
+ + L ++++G+++ +
Sbjct: 530 ENSAAVLVHLSSGDQQYL 583
>AV420018
Length = 213
Score = 31.2 bits (69), Expect = 0.38
Identities = 20/59 (33%), Positives = 32/59 (53%)
Frame = +1
Query: 242 TQAIVNHGLLPCLLSLLTHPKKSIKKEACRTLSNITAGNREQIQAVIEAGLIAPLVNLL 300
T+AIV HG +P L+ L + + A L NI++ E Q + E G++ ++NLL
Sbjct: 22 TRAIVGHGGVPPLIELCQSGESVSQAAAACALKNISS-VPEVRQVLAEEGVVKVMINLL 195
>TC18720 weakly similar to UP|SCWA_YEAST (Q04951) Probable family 17
glucosidase SCW10 precursor (Soluble cell wall protein
10) , partial (7%)
Length = 871
Score = 30.0 bits (66), Expect = 0.85
Identities = 59/277 (21%), Positives = 110/277 (39%), Gaps = 19/277 (6%)
Frame = +3
Query: 42 RFVQFLDKGDFPQLQLEAAWALTNIAAGTSENTKVVVDHGAVPMFVKLLSSPCDDVRGQA 101
R V L +GD + EAA + +A S + GA+P +L S D + +
Sbjct: 69 RLVAELHQGDDTVKRREAAATVRMLAKEDSHVRGTLAMLGAIPPLGAMLDSEDLDSQKAS 248
Query: 102 AWALGNIAGDSPRGRDLVLSHGALILLLSQLNEQEGL-YRLRNAVWTLSNFCRGKPQPAL 160
+AL N+ + + ++ G++ +L + EGL R+ A+ ++NF
Sbjct: 249 LYALLNLGIGNDANKAAIVKVGSVHKMLKLIESPEGLDSRVSEAI--VANFLG------- 401
Query: 161 EQVRSALPALKCLVFSKDEV---VLTEACWALSYLSDGTSDNIQAVIEAGVCGRLVQILL 217
SAL + K ++ S V V T + S D ++A+ + + +L
Sbjct: 402 ---LSALDSNKPIIGSSAAVPFLVRTLQSSDVKTSSQAKQDALRALYNLSIFPGNISFIL 572
Query: 218 EPSLSALF-----------PALRTVGNIVSGDDMQTQAIVNHGLLPCLLSLLTHPKKSIK 266
E L +L + N+VS + + P L+ +L
Sbjct: 573 ETHLVPFLINSIGDMEVTERSLSILSNLVSTREGRKAISAVPDTFPILVDVLNWTDSPEC 752
Query: 267 KE----ACRTLSNITAGNREQIQAVIEAGLIAPLVNL 299
+E +++ + G+R Q +IEAG+++ L+ L
Sbjct: 753 QEKTSYVLMVMAHKSFGDR---QGMIEAGIVSSLLEL 854
>AV410889
Length = 436
Score = 28.1 bits (61), Expect = 3.2
Identities = 12/58 (20%), Positives = 29/58 (49%)
Frame = +1
Query: 373 NTGDVNLYAQTIKDVEGLEKGLEKFLKVGEAEKRFGNTGDVNLYAQMIEDVKGLEKIE 430
N GD+ + + ++ + L+ + + + ++GD+ + +I V+GLE+ E
Sbjct: 256 NHGDLQFFIKQLRSFRRFKHALQISEWMSDERNHYLHSGDIAIRLDLISKVRGLEEAE 429
>CN825562
Length = 658
Score = 28.1 bits (61), Expect = 3.2
Identities = 23/82 (28%), Positives = 37/82 (45%), Gaps = 12/82 (14%)
Frame = +3
Query: 46 FLDKGDFP----------QLQLEAAWALTNIAAGTSENTKVVVDHGAV-PMFVKLLSSPC 94
F+DK DF Q++++A+ TN+ + E V +G + P L+S
Sbjct: 93 FIDKKDFMSNIEDNKELGQMEVDASAGSTNMPSPQKEEEVVKKKYGGMMPKKPPLISKDH 272
Query: 95 DDVRGQAA-WALGNIAGDSPRG 115
+ +A WALG G P+G
Sbjct: 273 ERAYFDSADWALGKQGGGKPKG 338
>AV422643
Length = 469
Score = 26.9 bits (58), Expect = 7.2
Identities = 32/138 (23%), Positives = 54/138 (38%), Gaps = 5/138 (3%)
Frame = +2
Query: 188 ALSYLSDGTSDNIQAVIEAGVCGRLVQILLEPSLSALFPALRTVGNIV----SGDDMQTQ 243
AL L+ G+ N + + E L +I+ + F L T+ I S D++
Sbjct: 53 ALWMLARGSVSNSRKITETKGMLCLAKIIEKEQGELQFNCLMTIMEIAAAAESNADLRRA 232
Query: 244 AI-VNHGLLPCLLSLLTHPKKSIKKEACRTLSNITAGNREQIQAVIEAGLIAPLVNLLQN 302
A N ++ L K + + + + + G+ + E +I PLV L N
Sbjct: 233 AFKTNSPAAKAVVEQLLRIIKEVDSPSLQIPAIKSIGSLARTFPARETRMIEPLVMKLSN 412
Query: 303 AEFDITKEVAQALTNVTS 320
+ D+ E ALT S
Sbjct: 413 RDTDVADEADVALTKFAS 466
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.317 0.135 0.395
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,431,814
Number of Sequences: 28460
Number of extensions: 92874
Number of successful extensions: 469
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 452
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 461
length of query: 466
length of database: 4,897,600
effective HSP length: 93
effective length of query: 373
effective length of database: 2,250,820
effective search space: 839555860
effective search space used: 839555860
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 57 (26.6 bits)
Medicago: description of AC122160.6