BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC122160.5 - phase: 0
(433 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC14114 similar to UP|FD62_SOYBN (P48631) Omega-6 fatty acid des... 95 2e-20
BP042120 76 9e-15
BI417403 61 4e-10
BP033409 40 0.001
CB828626 33 0.070
BP028972 31 0.45
AU240113 30 1.0
AV778312 30 1.0
AU088765 29 1.3
TC14075 homologue to UP|PRP2_MEDTR (Q40375) Repetitive proline-r... 29 1.7
TC8090 homologue to UP|ACTB_ARATH (P53496) Actin 11, complete 29 1.7
TC15120 similar to UP|Q9FR82 (Q9FR82) Delta 8-sphingolipid desat... 28 2.3
TC9704 similar to UP|AAQ10732 (AAQ10732) Delta-8-sphingolipid de... 28 2.3
TC14301 28 2.3
AV427405 28 2.3
BP030245 28 3.8
TC8566 similar to UP|Q8LB96 (Q8LB96) Delta-8 sphingolipid desatu... 28 3.8
BI420403 27 5.0
TC10738 homologue to GB|AAO64838.1|29028918|BT005903 At4g16120 {... 27 6.6
TC8091 homologue to UP|ACT3_SOLTU (P30167) Actin 58, partial (39%) 27 6.6
>TC14114 similar to UP|FD62_SOYBN (P48631) Omega-6 fatty acid desaturase,
endoplasmic reticulum isozyme 2 , complete
Length = 1634
Score = 95.1 bits (235), Expect = 2e-20
Identities = 83/336 (24%), Positives = 144/336 (42%), Gaps = 40/336 (11%)
Frame = +1
Query: 86 PLPDNVTLKDVITSLP--KKVFEIDDVK------AWKSVLISATSYALGLLMISKAPWYL 137
P P+ + D + +P K F + VK +K ++ + SY + L I+ +Y+
Sbjct: 106 PQPNRKSETDAVKRVPFEKPPFSLSQVKKAIPPHCFKRSILRSFSYVVYDLTIAFFLYYV 285
Query: 138 L----------------PLAWAWTGTAVTGFFVIGHDCAHKSFSTNKLVEDIVGTLAFLP 181
P WA G +TG +VI H+C H +FS + ++D VG +
Sbjct: 286 ATHYFHLLPRPLAFLAWPTYWAIQGCILTGVWVIAHECGHHAFSDYQWLDDTVGLILHSS 465
Query: 182 LIYPYEPWRFKHDKHHAKTNMLKEDTAWQPV------WQDEFESSP---FLRKAIIYGYG 232
L+ PY W++ H +HH+ T L D + P W ++ ++P FL I G
Sbjct: 466 LLVPYFSWKYSHRRHHSNTGSLDRDEVFVPKQKSSIGWYSKYLNNPLGRFLTITITLTLG 645
Query: 233 -PFRCWMSIA----HWLVSHFDL--KTFRPNEIKRVKISLACVFAFIAIGWPLIIYKTGI 285
P +++ HFD + E ++ IS V A + G + G+
Sbjct: 646 WPLYLTFNVSGRPYERFACHFDPYGPIYSDRERLQIYISDVGVLA-VCFGLYHLAVAKGL 822
Query: 286 MGWIKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKNSEEWNAAQAQLNGTVHCAYPQWIEI 345
+ + +P L + ++ T + HT P +P +S EW+ + L TV Y ++
Sbjct: 823 AWVVCVYGVPLLVVNGFLVLITYLQHTHPALPHYDSSEWDWFRGAL-ATVDRDYGILNKV 999
Query: 346 LCHDINVHIPHHISPKIPSYNLRAAHKSIQENWGKY 381
+ + HI HH+ +P Y+ A K+I+ G+Y
Sbjct: 1000FHNITDTHIAHHLFSTMPHYHAMEATKAIKPILGEY 1107
>BP042120
Length = 473
Score = 76.3 bits (186), Expect = 9e-15
Identities = 31/34 (91%), Positives = 33/34 (96%)
Frame = -1
Query: 309 VHHTAPHIPFKNSEEWNAAQAQLNGTVHCAYPQW 342
VHHTAPHIPFK+SE+WNAAQAQLNGTVHC YPQW
Sbjct: 473 VHHTAPHIPFKDSEKWNAAQAQLNGTVHCDYPQW 372
>BI417403
Length = 460
Score = 60.8 bits (146), Expect = 4e-10
Identities = 27/70 (38%), Positives = 36/70 (50%)
Frame = +2
Query: 124 ALGLLMISKAPWYLLPLAWAWTGTAVTGFFVIGHDCAHKSFSTNKLVEDIVGTLAFLPLI 183
ALG + W PL WA GT FV+GHDC H SFS + ++ +VG + ++
Sbjct: 245 ALGAAALHFNNWLFWPLYWAAQGTMFWALFVLGHDCGHGSFSDSLMLNSVVGHILHSFIL 424
Query: 184 YPYEPWRFKH 193
PY WR H
Sbjct: 425 VPYHGWRISH 454
>BP033409
Length = 480
Score = 39.7 bits (91), Expect = 0.001
Identities = 15/43 (34%), Positives = 26/43 (59%)
Frame = -2
Query: 342 WIEILCHDINVHIPHHISPKIPSYNLRAAHKSIQENWGKYLNE 384
W+ + HDI H+ HH+ P++P Y+L A ++ + G+Y E
Sbjct: 464 WVNNIHHDIGTHVIHHLFPQMPHYHLVEATQAAKPVLGEYYRE 336
>CB828626
Length = 505
Score = 33.5 bits (75), Expect = 0.070
Identities = 14/32 (43%), Positives = 20/32 (61%)
Frame = +1
Query: 353 HIPHHISPKIPSYNLRAAHKSIQENWGKYLNE 384
H+ HH+ P+IP YNL A ++ + GKY E
Sbjct: 1 HVIHHLFPQIPHYNLIEATEAAKPVLGKYYRE 96
>BP028972
Length = 413
Score = 30.8 bits (68), Expect = 0.45
Identities = 12/16 (75%), Positives = 15/16 (93%)
Frame = -3
Query: 418 ESRPIRFLKKVMPDYA 433
+S+PI+FLK VMPDYA
Sbjct: 411 DSQPIKFLKNVMPDYA 364
>AU240113
Length = 300
Score = 29.6 bits (65), Expect = 1.0
Identities = 19/57 (33%), Positives = 22/57 (38%), Gaps = 10/57 (17%)
Frame = +2
Query: 296 WLGYHFWMSTFTMVHHTAPHIPFKNSEEWNA-AQAQLNGTVHCAY---------PQW 342
W G+H W F + + P I N WN QLN V C Y PQW
Sbjct: 47 WTGHHHWRRAFPLP*SSLPAIHDWNGSCWNP*DHIQLNHEV*C*YQKGLVWKHSPQW 217
>AV778312
Length = 610
Score = 29.6 bits (65), Expect = 1.0
Identities = 14/35 (40%), Positives = 19/35 (54%)
Frame = +3
Query: 48 NKVTVIRAVAIPVEPAPVESAEYRKQLAERYGFEQ 82
N T R + +PAP+ YR+Q AER G E+
Sbjct: 372 NSTTPSRLYLLREKPAPLPRRRYRRQTAERRGSEE 476
>AU088765
Length = 423
Score = 29.3 bits (64), Expect = 1.3
Identities = 11/26 (42%), Positives = 17/26 (65%)
Frame = +3
Query: 371 HKSIQENWGKYLNEASWNWRLMKTIM 396
HKSIQ ++ K+L+ W W L T++
Sbjct: 75 HKSIQRSFKKFLHFLFWLWSLQSTLL 152
>TC14075 homologue to UP|PRP2_MEDTR (Q40375) Repetitive proline-rich cell wall
protein 2 precursor, partial (98%)
Length = 1524
Score = 28.9 bits (63), Expect = 1.7
Identities = 11/37 (29%), Positives = 20/37 (53%)
Frame = -1
Query: 274 IGWPLIIYKTGIMGWIKFWLMPWLGYHFWMSTFTMVH 310
+ W L+ ++ G I WL+ W H+W+ + +VH
Sbjct: 1029 LNWRLVNWRFLNWGLINRWLLYWGLVHWWLVNWGLVH 919
Score = 26.9 bits (58), Expect = 6.6
Identities = 10/37 (27%), Positives = 19/37 (51%)
Frame = -1
Query: 274 IGWPLIIYKTGIMGWIKFWLMPWLGYHFWMSTFTMVH 310
+ W L+ + G + +WL+ W H W+ + +VH
Sbjct: 819 LNWWLVNWWLFNWGLVNWWLLNWGLVHRWLLNWGLVH 709
>TC8090 homologue to UP|ACTB_ARATH (P53496) Actin 11, complete
Length = 1624
Score = 28.9 bits (63), Expect = 1.7
Identities = 19/65 (29%), Positives = 27/65 (41%), Gaps = 3/65 (4%)
Frame = +1
Query: 296 WLGYHFWMSTFTMVHHTAPHIPFKN-SEEWNAAQAQLNGTVHCAYPQ-WIEILC-HDINV 352
W G H W F M P IP +N S + Q + + C Y + ++ C N
Sbjct: 835 WAGNHNWRRAFQMSRGLVPTIPGRNGSSRHSRDNIQFHNEMRC*YQERFVR*HCPFWRNN 1014
Query: 353 HIPHH 357
H+P H
Sbjct: 1015HVPRH 1029
>TC15120 similar to UP|Q9FR82 (Q9FR82) Delta 8-sphingolipid desaturase,
partial (37%)
Length = 776
Score = 28.5 bits (62), Expect = 2.3
Identities = 10/41 (24%), Positives = 21/41 (50%)
Frame = +2
Query: 328 QAQLNGTVHCAYPQWIEILCHDINVHIPHHISPKIPSYNLR 368
+ Q +GT+ + W++ + + HH+ P++P LR
Sbjct: 206 EKQTSGTLDISCSSWMDWFFGGLQFQLEHHLFPRLPRAQLR 328
>TC9704 similar to UP|AAQ10732 (AAQ10732) Delta-8-sphingolipid desaturase,
partial (25%)
Length = 848
Score = 28.5 bits (62), Expect = 2.3
Identities = 10/41 (24%), Positives = 22/41 (53%)
Frame = +3
Query: 328 QAQLNGTVHCAYPQWIEILCHDINVHIPHHISPKIPSYNLR 368
+ Q +GT+ + W++ + + HH+ P++P +LR
Sbjct: 45 EKQTHGTLDVSCSPWMDWFHGGLQFQVEHHLFPRLPRCHLR 167
>TC14301
Length = 683
Score = 28.5 bits (62), Expect = 2.3
Identities = 11/27 (40%), Positives = 17/27 (62%)
Frame = -2
Query: 326 AAQAQLNGTVHCAYPQWIEILCHDINV 352
A+Q L G C+YPQWI + C +++
Sbjct: 547 ASQKLLQGQGICSYPQWIVLWCLTVDL 467
>AV427405
Length = 430
Score = 28.5 bits (62), Expect = 2.3
Identities = 19/67 (28%), Positives = 32/67 (47%), Gaps = 11/67 (16%)
Frame = -3
Query: 292 WLMPWLGYHFWMSTFTMVHH---------TAPH--IPFKNSEEWNAAQAQLNGTVHCAYP 340
W + W+G F + TF +VH+ T P IP++ +W+ + T+H + P
Sbjct: 197 WRIVWVGDCFCLLTFEIVHNIDYTPNPFITRPAYCIPWRP*YQWHGC---ADTTIHVSAP 27
Query: 341 QWIEILC 347
I +LC
Sbjct: 26 *LISLLC 6
>BP030245
Length = 230
Score = 27.7 bits (60), Expect = 3.8
Identities = 8/19 (42%), Positives = 12/19 (63%)
Frame = +1
Query: 296 WLGYHFWMSTFTMVHHTAP 314
++ YH+W FT+ HH P
Sbjct: 16 YMDYHYWTIIFTLHHHQTP 72
>TC8566 similar to UP|Q8LB96 (Q8LB96) Delta-8 sphingolipid desaturase,
partial (63%)
Length = 1081
Score = 27.7 bits (60), Expect = 3.8
Identities = 30/108 (27%), Positives = 47/108 (42%), Gaps = 7/108 (6%)
Frame = +2
Query: 155 IGHDCAH----KSFSTNKLVEDIVGT-LAFLPLIYPYEPWRFKHDKHHAKTNMLKEDTAW 209
+GHD H S NKL + + G + + + + W++ H+ HH N L D
Sbjct: 686 VGHDSGHYEVMSSRGYNKLAQVLCGNCMTGISIAW----WKWTHNAHHIACNSLDYDPDL 853
Query: 210 Q--PVWQDEFESSPFLRKAIIYGYGPFRCWMSIAHWLVSHFDLKTFRP 255
Q PV+ SS F YG + +++ +L+S F TF P
Sbjct: 854 QHIPVFA---VSSRFFSSIRSCFYGRELKFDALSRFLIS-FQHWTFYP 985
>BI420403
Length = 425
Score = 27.3 bits (59), Expect = 5.0
Identities = 17/52 (32%), Positives = 23/52 (43%), Gaps = 4/52 (7%)
Frame = +3
Query: 359 SPKIPSYNLRAAHKSIQENWGKYLNEASWN----WRLMKTIMTVCHVYDKQQ 406
S ++ +NLR H NW LN AS W+ K + HV D +Q
Sbjct: 204 SSQLGCHNLRTKHSVFALNWENQLNRASLGGENPWKAPK---IMAHVVDHRQ 350
>TC10738 homologue to GB|AAO64838.1|29028918|BT005903 At4g16120 {Arabidopsis
thaliana;}, partial (5%)
Length = 596
Score = 26.9 bits (58), Expect = 6.6
Identities = 14/35 (40%), Positives = 15/35 (42%)
Frame = -1
Query: 133 APWYLLPLAWAWTGTAVTGFFVIGHDCAHKSFSTN 167
+PW LP AW F I D KS STN
Sbjct: 122 SPWKALPETTAWNH*RKRAFLSIEEDFRGKSISTN 18
>TC8091 homologue to UP|ACT3_SOLTU (P30167) Actin 58, partial (39%)
Length = 640
Score = 26.9 bits (58), Expect = 6.6
Identities = 19/66 (28%), Positives = 27/66 (40%), Gaps = 4/66 (6%)
Frame = +3
Query: 296 WLGYHFWMSTFTMVHHTAPHIPFKNSEEWNAA-QAQLNGTVHCAY---PQWIEILCHDIN 351
W G+H+W F + P IP N N+ QL+ + C + P W N
Sbjct: 51 WAGHHYWC*AF*VSRGLVPTIPGGNGSCRNSRDNIQLHNEM*C*HQERPVW*HCSLRRYN 230
Query: 352 VHIPHH 357
H+P H
Sbjct: 231 -HVPWH 245
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.323 0.138 0.461
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,642,402
Number of Sequences: 28460
Number of extensions: 159644
Number of successful extensions: 958
Number of sequences better than 10.0: 44
Number of HSP's better than 10.0 without gapping: 940
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 955
length of query: 433
length of database: 4,897,600
effective HSP length: 93
effective length of query: 340
effective length of database: 2,250,820
effective search space: 765278800
effective search space used: 765278800
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 57 (26.6 bits)
Medicago: description of AC122160.5