BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC122160.3 - phase: 0 /pseudo
(835 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV780358 223 1e-58
TC18965 similar to UP|Q9FPS8 (Q9FPS8) Ubiquitin-specific proteas... 99 3e-21
TC8806 similar to GB|AAF21246.1|6648604|AF048705 ubiquitin-speci... 63 2e-10
AV406535 62 5e-10
TC16220 similar to UP|Q9ZSB5 (Q9ZSB5) F3H7.5 protein, partial (6%) 48 7e-06
AV779158 43 2e-04
BP064143 41 7e-04
TC11632 similar to UP|Q9FPT4 (Q9FPT4) Ubiquitin-specific proteas... 39 0.004
TC13353 similar to UP|Q9FPS1 (Q9FPS1) Ubiquitin-specific proteas... 35 0.064
AV778304 34 0.083
TC13345 similar to UP|Q9MAQ3 (Q9MAQ3) CDS, partial (3%) 33 0.14
TC15450 similar to GB|AAD04752.1|4098989|ATU81498 phenylalkylami... 31 0.92
TC16806 30 1.6
TC8877 similar to UP|O65917 (O65917) Dehydroquinate dehydratase/... 30 2.1
BP056532 29 2.7
BP075330 28 4.6
BP076542 28 7.8
>AV780358
Length = 599
Score = 223 bits (568), Expect = 1e-58
Identities = 114/169 (67%), Positives = 134/169 (78%), Gaps = 7/169 (4%)
Frame = +2
Query: 6 MVETETITKQHDP------FQRKIEFIPVKKPFKGFSNDFNIETLNPTTSEHRQLVSANI 59
M E +T T DP F RKIEF+P++KPF GFSNDF++ETLNP+TS + S
Sbjct: 89 MAEIQTETPPPDPAQSSSLFSRKIEFVPIRKPFNGFSNDFHLETLNPSTSGAARKPS--- 259
Query: 60 QSSQPQPSKNSEFSEFGLDPELSLGI-TFRRIGAGLWNLGNTCFLNSVLQCLTYTEPLAA 118
S+ +SE+S+FGLDPELS G+ TFRRIGAGL NLGNTCFLNSVLQCLTYTEPLAA
Sbjct: 260 -SASALKKHDSEYSDFGLDPELSFGMMTFRRIGAGLQNLGNTCFLNSVLQCLTYTEPLAA 436
Query: 119 YLQSGKHKSSCHIAGFCALCAIQNHVSCALQATGRIVSPQDMVGNLRCI 167
YL+SGKHK+SC +AGFCALCAIQNHVS ALQA+G+I+SPQ +VGNLRCI
Sbjct: 437 YLESGKHKTSCRVAGFCALCAIQNHVSRALQASGKILSPQALVGNLRCI 583
>TC18965 similar to UP|Q9FPS8 (Q9FPS8) Ubiquitin-specific protease 16,
partial (17%)
Length = 557
Score = 99.0 bits (245), Expect = 3e-21
Identities = 59/129 (45%), Positives = 80/129 (61%), Gaps = 3/129 (2%)
Frame = +1
Query: 237 SNKFDPFLDLSLEIL-KADSLQKALANFTAAELLDGGEKQYHCQRCKQKVQAVKQLTIHK 295
S + + +DL++EI + +L +AL FT+ E LDG E +YHC RCK +A K+LTI +
Sbjct: 175 SERHERMMDLTVEIEGEITTLVEALRRFTSTEALDG-ENKYHCDRCKSYEKAKKKLTISE 351
Query: 296 APYVLAIHLKRFYAHDPNIKIKKKVRFDSALNLKPFVSGSSDGDVKYSLYGVLVHSGF-- 353
AP VL I LKRF + K+ K ++F L+L PFVSG+SD Y LYGV+VH
Sbjct: 352 APNVLTIALKRFQSGKFG-KLNKPIQFPEILDLAPFVSGTSDKSPIYRLYGVVVHLDIMN 528
Query: 354 STHSGHYYC 362
+ SGHY C
Sbjct: 529 AAFSGHYVC 555
>TC8806 similar to GB|AAF21246.1|6648604|AF048705 ubiquitin-specific
protease {Arabidopsis thaliana;}, partial (12%)
Length = 751
Score = 62.8 bits (151), Expect = 2e-10
Identities = 40/114 (35%), Positives = 60/114 (52%), Gaps = 4/114 (3%)
Frame = +2
Query: 290 QLTIHKAPYVLAIHLKRF-YAHDPNIKIKKKVRFD-SALNLKPFVSGSSDGDVK-YSLYG 346
+L + + P VL IHLKRF Y+ K++ V +L +++ +D + Y LY
Sbjct: 2 KLDLWRLPEVLFIHLKRFSYSRSMKHKLETFVNLPIHDFDLTSYIANENDSRPQLYELYA 181
Query: 347 VLVHSGFSTHSGHYYCYVRT-SNNMWYTLDDTRVSHVGEQEVLNQQAYMLFYVR 399
+ H G + SGHY +++ N WY DDT +S + E EV AY+LFY R
Sbjct: 182 LTNHYG-TLGSGHYTAHIKLLDENRWYNFDDTHISAISEDEVNTAAAYVLFYRR 340
>AV406535
Length = 432
Score = 61.6 bits (148), Expect = 5e-10
Identities = 30/72 (41%), Positives = 43/72 (59%)
Frame = +2
Query: 93 GLWNLGNTCFLNSVLQCLTYTEPLAAYLQSGKHKSSCHIAGFCALCAIQNHVSCALQATG 152
GL N GN+CF N VLQCL++T+PL AYL H+ C +C LC + HV A + +
Sbjct: 185 GLLNCGNSCFANVVLQCLSFTKPLIAYLLEKGHRKECGHNDWCFLCEFEIHVERA-RLSS 361
Query: 153 RIVSPQDMVGNL 164
+ SP +++ L
Sbjct: 362 QAFSPMNILSRL 397
>TC16220 similar to UP|Q9ZSB5 (Q9ZSB5) F3H7.5 protein, partial (6%)
Length = 757
Score = 47.8 bits (112), Expect = 7e-06
Identities = 24/60 (40%), Positives = 32/60 (53%), Gaps = 1/60 (1%)
Frame = +2
Query: 341 KYSLYGVLVHSGFSTHSGHYYCYVRT-SNNMWYTLDDTRVSHVGEQEVLNQQAYMLFYVR 399
KY+LY + H G GHY Y + N WY DD+ V+ E E+ + AY+LFY R
Sbjct: 17 KYNLYAISNHYG-GLGGGHYTAYAKLIDENKWYHFDDSHVTPADEAEIKSSAAYVLFYQR 193
>AV779158
Length = 424
Score = 43.1 bits (100), Expect = 2e-04
Identities = 26/68 (38%), Positives = 31/68 (45%), Gaps = 12/68 (17%)
Frame = -3
Query: 342 YSLYGVLVHSGFSTHSGHYYCYVR------------TSNNMWYTLDDTRVSHVGEQEVLN 389
Y L GV+ HSG + GHY YVR + WY D V V EVL
Sbjct: 401 YHLLGVVEHSG-TMRGGHYLAYVRGGPRHQGNADKENERSTWYHASDAYVRPVSLDEVLR 225
Query: 390 QQAYMLFY 397
+AY+LFY
Sbjct: 224 CEAYLLFY 201
>BP064143
Length = 466
Score = 41.2 bits (95), Expect = 7e-04
Identities = 22/63 (34%), Positives = 30/63 (46%)
Frame = -3
Query: 337 DGDVKYSLYGVLVHSGFSTHSGHYYCYVRTSNNMWYTLDDTRVSHVGEQEVLNQQAYMLF 396
DG KY L GV+ HSG S H GHY ++ + W +D +V + Y+ F
Sbjct: 371 DGGGKYRLMGVVSHSGTSVHCGHYVAHI-LKDGRWVIFNDDQVG--ASISPPKEMGYLYF 201
Query: 397 YVR 399
Y R
Sbjct: 200 YER 192
>TC11632 similar to UP|Q9FPT4 (Q9FPT4) Ubiquitin-specific protease 6,
partial (16%)
Length = 612
Score = 38.5 bits (88), Expect = 0.004
Identities = 20/63 (31%), Positives = 27/63 (42%), Gaps = 7/63 (11%)
Frame = +1
Query: 342 YSLYGVLVHSGFSTHSGHYYCYVRTSNNMWYTLDDTRVSHVGEQEVLN-------QQAYM 394
Y L VL H G S SGHY +V+ N W DD E+++ AY+
Sbjct: 88 YDLVAVLTHKGRSADSGHYVGWVKQENGKWIEFDDDNPKPRLEEDITKLSGGGDWHMAYI 267
Query: 395 LFY 397
+ Y
Sbjct: 268 IMY 276
>TC13353 similar to UP|Q9FPS1 (Q9FPS1) Ubiquitin-specific protease 26,
partial (11%)
Length = 532
Score = 34.7 bits (78), Expect = 0.064
Identities = 22/44 (50%), Positives = 26/44 (59%), Gaps = 1/44 (2%)
Frame = +2
Query: 68 KNSEFSE-FGLDPELSLGITFRRIGAGLWNLGNTCFLNSVLQCL 110
K S+F E G DP L + AGL NLG TC+ NS+LQCL
Sbjct: 368 KMSDFVESLGPDPNKDLRESADS-PAGLTNLGATCYANSILQCL 496
>AV778304
Length = 563
Score = 34.3 bits (77), Expect = 0.083
Identities = 21/70 (30%), Positives = 32/70 (45%)
Frame = +3
Query: 345 YGVLVHSGFSTHSGHYYCYVRTSNNMWYTLDDTRVSHVGEQEVLNQQAYMLFYVRDKKSI 404
Y LV FSTHS H++ Y + +D+ V ++ + + Q DK +
Sbjct: 261 YTCLVDWHFSTHSTHHFLYTIR*RVVQVCIDNVNVKYLPREGIRRTQK---VGCEDKSFL 431
Query: 405 TPRKPVNIAK 414
PRK +NI K
Sbjct: 432 PPRKAMNIGK 461
>TC13345 similar to UP|Q9MAQ3 (Q9MAQ3) CDS, partial (3%)
Length = 572
Score = 33.5 bits (75), Expect = 0.14
Identities = 13/27 (48%), Positives = 16/27 (59%)
Frame = +3
Query: 371 WYTLDDTRVSHVGEQEVLNQQAYMLFY 397
WY DD RV+ E E+ AY+LFY
Sbjct: 138 WYEFDDPRVAPANEDEIKTSAAYVLFY 218
>TC15450 similar to GB|AAD04752.1|4098989|ATU81498 phenylalkylamine binding
protein homolog {Arabidopsis thaliana;} , partial (70%)
Length = 1088
Score = 30.8 bits (68), Expect = 0.92
Identities = 14/41 (34%), Positives = 19/41 (46%)
Frame = +2
Query: 334 GSSDGDVKYSLYGVLVHSGFSTHSGHYYCYVRTSNNMWYTL 374
G G Y + +L FSTHS +YY Y +N W +
Sbjct: 521 GQLYGTAVYYITAILEGDNFSTHSFYYYAYYIGANFSWIVI 643
>TC16806
Length = 488
Score = 30.0 bits (66), Expect = 1.6
Identities = 17/57 (29%), Positives = 23/57 (39%)
Frame = +1
Query: 341 KYSLYGVLVHSGFSTHSGHYYCYVRTSNNMWYTLDDTRVSHVGEQEVLNQQAYMLFY 397
+Y L LVH G + +R S +WY + D VS V + YM Y
Sbjct: 7 RYDLIAHLVHDGKPGEGLYRVFVLRQSEALWYEMQDLHVSVTLPHLVALSETYMQIY 177
>TC8877 similar to UP|O65917 (O65917) Dehydroquinate
dehydratase/shikimate:NADP oxidoreductase
(3-dehydroquinate dehydratase) , partial (15%)
Length = 661
Score = 29.6 bits (65), Expect = 2.1
Identities = 17/49 (34%), Positives = 24/49 (48%), Gaps = 2/49 (4%)
Frame = -3
Query: 122 SGKHKSSCHIAGFCALCAIQ--NHVSCALQATGRIVSPQDMVGNLRCIL 168
S KH SC+ +G C C +Q +H C + V Q M+ N RC +
Sbjct: 197 SNKHLQSCYNSGSCFFCFLQESSHFRCVDSIKDKRVKFQRMLRN-RCFI 54
>BP056532
Length = 278
Score = 29.3 bits (64), Expect = 2.7
Identities = 12/21 (57%), Positives = 17/21 (80%)
Frame = -1
Query: 106 VLQCLTYTEPLAAYLQSGKHK 126
V++ LT +PL+AYL +GKHK
Sbjct: 101 VVRVLTRVDPLSAYLVAGKHK 39
>BP075330
Length = 452
Score = 28.5 bits (62), Expect = 4.6
Identities = 13/32 (40%), Positives = 19/32 (58%)
Frame = -1
Query: 262 NFTAAELLDGGEKQYHCQRCKQKVQAVKQLTI 293
N++A ++L GGE+Q HC R Q + L I
Sbjct: 119 NYSAVKVL*GGERQLHCIRDSVLYQCIGNLFI 24
>BP076542
Length = 376
Score = 27.7 bits (60), Expect = 7.8
Identities = 10/21 (47%), Positives = 16/21 (75%)
Frame = -3
Query: 678 KKKFPKYKGSIMQLSLIRPSM 698
KKKFPK G +M+ S+++P +
Sbjct: 284 KKKFPKMDGFVMEKSVLKPEI 222
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.320 0.134 0.397
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 14,211,928
Number of Sequences: 28460
Number of extensions: 203099
Number of successful extensions: 1039
Number of sequences better than 10.0: 34
Number of HSP's better than 10.0 without gapping: 1033
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1035
length of query: 835
length of database: 4,897,600
effective HSP length: 98
effective length of query: 737
effective length of database: 2,108,520
effective search space: 1553979240
effective search space used: 1553979240
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 59 (27.3 bits)
Medicago: description of AC122160.3