BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC121245.5 + phase: 0
(72 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC18647 similar to UP|Q9ASR9 (Q9ASR9) At2g01320/F10A8.20, partia... 54 7e-09
BP029778 40 6e-05
AV407220 39 1e-04
AW720269 38 4e-04
TC10604 similar to AAQ89671 (AAQ89671) At4g33460, partial (36%) 36 0.002
TC10978 similar to UP|Q93YY0 (Q93YY0) 68 kDa protein HP68, parti... 35 0.003
AV413975 35 0.003
TC16623 weakly similar to UP|TAP2_HUMAN (Q03519) Antigen peptide... 31 0.039
TC11559 similar to UP|Q9SXT3 (Q9SXT3) Multidrug resistance prote... 31 0.039
BP042433 31 0.039
TC9999 similar to UP|Q9FMU0 (Q9FMU0) Similarity to ABC transport... 30 0.086
AV417384 28 0.33
TC18135 homologue to UP|Q9MAY4 (Q9MAY4) ABC transporter homolog,... 26 1.2
AV406443 26 1.2
AV428934 25 2.8
AV420070 25 2.8
CB828788 24 4.7
TC17658 homologue to UP|CLPA_PEA (P35100) ATP-dependent Clp prot... 24 4.7
TC10260 similar to UP|Q9MA20 (Q9MA20) T5E21.13, partial (6%) 24 6.2
TC10596 24 6.2
>TC18647 similar to UP|Q9ASR9 (Q9ASR9) At2g01320/F10A8.20, partial (17%)
Length = 430
Score = 53.5 bits (127), Expect = 7e-09
Identities = 26/60 (43%), Positives = 38/60 (63%)
Frame = +1
Query: 8 EVDGTCLTWKDIWVNTISNGKNGSKSILQGLTGYAKPGQLLAIMGPSGCGKSTLLDTLAG 67
+V + W +I + + +L+ ++G AKPG+LLAIMGPSG GK+TLL+ LAG
Sbjct: 28 KVTPVTIRWSNINCSFSDKSSKSVRFLLKNVSGEAKPGRLLAIMGPSGSGKTTLLNVLAG 207
>BP029778
Length = 438
Score = 40.4 bits (93), Expect = 6e-05
Identities = 18/34 (52%), Positives = 24/34 (69%)
Frame = -2
Query: 34 ILQGLTGYAKPGQLLAIMGPSGCGKSTLLDTLAG 67
+L ++G PG L A+MG SG GK+TL+D LAG
Sbjct: 278 LLSNVSGVFAPGVLTALMGSSGAGKTTLMDVLAG 177
>AV407220
Length = 235
Score = 39.3 bits (90), Expect = 1e-04
Identities = 19/33 (57%), Positives = 28/33 (84%), Gaps = 1/33 (3%)
Frame = +3
Query: 14 LTWKDIWVN-TISNGKNGSKSILQGLTGYAKPG 45
LTWKD+ V T+SNG+ ++++L+GLTGYA+PG
Sbjct: 141 LTWKDLTVMVTLSNGE--TQNVLEGLTGYAEPG 233
>AW720269
Length = 524
Score = 37.7 bits (86), Expect = 4e-04
Identities = 17/38 (44%), Positives = 28/38 (72%)
Frame = +3
Query: 33 SILQGLTGYAKPGQLLAIMGPSGCGKSTLLDTLAGTNK 70
+IL+ ++ A+ +++A++GPSG GKSTLL +AG K
Sbjct: 51 NILKSVSFVARSSEIVAVVGPSGTGKSTLLRIIAGRVK 164
>TC10604 similar to AAQ89671 (AAQ89671) At4g33460, partial (36%)
Length = 498
Score = 35.8 bits (81), Expect = 0.002
Identities = 15/23 (65%), Positives = 18/23 (78%)
Frame = +2
Query: 45 GQLLAIMGPSGCGKSTLLDTLAG 67
GQ ++GP+GCGKSTLL LAG
Sbjct: 233 GQFWMLLGPNGCGKSTLLKILAG 301
>TC10978 similar to UP|Q93YY0 (Q93YY0) 68 kDa protein HP68, partial (25%)
Length = 488
Score = 35.0 bits (79), Expect = 0.003
Identities = 16/30 (53%), Positives = 21/30 (69%)
Frame = +1
Query: 43 KPGQLLAIMGPSGCGKSTLLDTLAGTNKLN 72
+PGQ+L ++G +G GKST L LAG K N
Sbjct: 322 RPGQVLGLVGTNGIGKSTALKVLAGKLKPN 411
>AV413975
Length = 343
Score = 35.0 bits (79), Expect = 0.003
Identities = 17/48 (35%), Positives = 28/48 (57%)
Frame = +2
Query: 18 DIWVNTISNGKNGSKSILQGLTGYAKPGQLLAIMGPSGCGKSTLLDTL 65
D+W S S +L+G+T PG +A++GPSG GK+T+ + +
Sbjct: 161 DVW---FSYPSRPSHPVLKGITMKLHPGSKVALVGPSGGGKTTIANLI 295
>TC16623 weakly similar to UP|TAP2_HUMAN (Q03519) Antigen peptide
transporter 2 (APT2) (Peptide transporter TAP2) (Peptide
transporter PSF2) (Peptide supply factor 2) (PSF-2)
(Peptide transporter involved in antigen processing 2),
partial (4%)
Length = 530
Score = 31.2 bits (69), Expect = 0.039
Identities = 15/36 (41%), Positives = 21/36 (57%)
Frame = +1
Query: 32 KSILQGLTGYAKPGQLLAIMGPSGCGKSTLLDTLAG 67
K +L+GL G++ A+MG +G GKST L G
Sbjct: 205 KDLLKGLNLTINRGEVHALMGANGSGKSTFAKVLVG 312
>TC11559 similar to UP|Q9SXT3 (Q9SXT3) Multidrug resistance protein
(Fragment), partial (41%)
Length = 460
Score = 31.2 bits (69), Expect = 0.039
Identities = 21/67 (31%), Positives = 32/67 (47%), Gaps = 4/67 (5%)
Frame = +3
Query: 3 REKEDEVDGTCL----TWKDIWVNTISNGKNGSKSILQGLTGYAKPGQLLAIMGPSGCGK 58
RE +VDG T + ++ + IL+G+ G ++ ++GPSG GK
Sbjct: 30 REHLVQVDGYAANGNATKPKFHITNLTKFSDDGVPILKGIHLDIPKGVIVGVIGPSGSGK 209
Query: 59 STLLDTL 65
STLL L
Sbjct: 210STLLRAL 230
>BP042433
Length = 534
Score = 31.2 bits (69), Expect = 0.039
Identities = 15/34 (44%), Positives = 20/34 (58%)
Frame = +2
Query: 34 ILQGLTGYAKPGQLLAIMGPSGCGKSTLLDTLAG 67
++ LT +PG L I GP+G GKS+L L G
Sbjct: 311 LVDDLTLRVEPGSNLLITGPNGSGKSSLFRVLGG 412
>TC9999 similar to UP|Q9FMU0 (Q9FMU0) Similarity to ABC transporter,
partial (43%)
Length = 656
Score = 30.0 bits (66), Expect = 0.086
Identities = 14/21 (66%), Positives = 15/21 (70%)
Frame = +3
Query: 50 IMGPSGCGKSTLLDTLAGTNK 70
I G SG GK+TLL LAG NK
Sbjct: 363 IFGQSGSGKTTLLQLLAGINK 425
>AV417384
Length = 380
Score = 28.1 bits (61), Expect = 0.33
Identities = 17/50 (34%), Positives = 28/50 (56%)
Frame = +2
Query: 21 VNTISNGKNGSKSILQGLTGYAKPGQLLAIMGPSGCGKSTLLDTLAGTNK 70
+N +S +N ++ IL+ + G L + G +G GKST L LAG ++
Sbjct: 209 LNNVSCMRN-AQQILRHVNVSLHDGGALVLTGANGSGKSTFLRMLAGFSR 355
>TC18135 homologue to UP|Q9MAY4 (Q9MAY4) ABC transporter homolog, partial
(50%)
Length = 968
Score = 26.2 bits (56), Expect = 1.2
Identities = 10/21 (47%), Positives = 15/21 (70%)
Frame = +3
Query: 45 GQLLAIMGPSGCGKSTLLDTL 65
G+ ++G +GCGKSTLL +
Sbjct: 375 GRRYGLLGLNGCGKSTLLTAI 437
>AV406443
Length = 417
Score = 26.2 bits (56), Expect = 1.2
Identities = 9/21 (42%), Positives = 16/21 (75%)
Frame = +3
Query: 47 LLAIMGPSGCGKSTLLDTLAG 67
++ ++GP G GKST+++ L G
Sbjct: 204 VIGVIGPPGVGKSTIMNELYG 266
>AV428934
Length = 323
Score = 25.0 bits (53), Expect = 2.8
Identities = 11/27 (40%), Positives = 17/27 (62%)
Frame = +1
Query: 34 ILQGLTGYAKPGQLLAIMGPSGCGKST 60
+L+ L +KP + L ++GP G GK T
Sbjct: 100 LLRRLKCSSKPDKRLILIGPPGSGKGT 180
>AV420070
Length = 403
Score = 25.0 bits (53), Expect = 2.8
Identities = 12/23 (52%), Positives = 14/23 (60%)
Frame = +1
Query: 44 PGQLLAIMGPSGCGKSTLLDTLA 66
PG L + G G GKSTLL +A
Sbjct: 7 PGSLTLVGGDPGVGKSTLLLQIA 75
>CB828788
Length = 448
Score = 24.3 bits (51), Expect = 4.7
Identities = 11/18 (61%), Positives = 14/18 (77%)
Frame = +2
Query: 48 LAIMGPSGCGKSTLLDTL 65
LAI+G GKSTL++TL
Sbjct: 140 LAIVGRPNVGKSTLMNTL 193
>TC17658 homologue to UP|CLPA_PEA (P35100) ATP-dependent Clp protease
ATP-binding subunit clpA homolog, chloroplast precursor
, partial (33%)
Length = 908
Score = 24.3 bits (51), Expect = 4.7
Identities = 14/39 (35%), Positives = 20/39 (50%), Gaps = 3/39 (7%)
Frame = +1
Query: 31 SKSILQGLTGYAKPGQLLA---IMGPSGCGKSTLLDTLA 66
S++I + G P + +A GP+G GKS L LA
Sbjct: 490 SRAIRRARVGLKNPNRPIASFIFSGPTGVGKSELAKALA 606
>TC10260 similar to UP|Q9MA20 (Q9MA20) T5E21.13, partial (6%)
Length = 730
Score = 23.9 bits (50), Expect = 6.2
Identities = 10/36 (27%), Positives = 20/36 (54%), Gaps = 3/36 (8%)
Frame = +3
Query: 19 IWVNTISNGKNGSKSILQG---LTGYAKPGQLLAIM 51
+W+ T+ N + G I QG L + +P +++ I+
Sbjct: 318 LWLKTVRNSRRGLLRITQGMPSLISFTRPIRIMLII 425
>TC10596
Length = 540
Score = 23.9 bits (50), Expect = 6.2
Identities = 10/20 (50%), Positives = 12/20 (60%)
Frame = +3
Query: 11 GTCLTWKDIWVNTISNGKNG 30
GT W +WVNT S G+ G
Sbjct: 264 GTTCVW-GVWVNTKSGGEGG 320
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.311 0.133 0.399
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,284,752
Number of Sequences: 28460
Number of extensions: 12745
Number of successful extensions: 65
Number of sequences better than 10.0: 42
Number of HSP's better than 10.0 without gapping: 65
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 65
length of query: 72
length of database: 4,897,600
effective HSP length: 48
effective length of query: 24
effective length of database: 3,531,520
effective search space: 84756480
effective search space used: 84756480
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 48 (23.1 bits)
Medicago: description of AC121245.5