BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC121245.12 + phase: 0 /pseudo
(136 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC11323 65 4e-12
BP036240 59 2e-10
BP080173 59 3e-10
BI418523 58 5e-10
BP056414 57 1e-09
TC19596 55 5e-09
AV407204 52 4e-08
TC13560 52 5e-08
BP069214 50 1e-07
BI417841 47 1e-06
TC11605 46 2e-06
AV780445 43 2e-05
BP033684 43 2e-05
TC11350 similar to UP|Q9LJP7 (Q9LJP7) Genomic DNA, chromosome 3,... 43 2e-05
BP053073 41 8e-05
AV780577 40 1e-04
TC10933 similar to UP|Q9LNS0 (Q9LNS0) F1L3.4, partial (6%) 33 0.017
BP052637 33 0.022
TC9793 32 0.029
BP062431 32 0.038
>TC11323
Length = 657
Score = 65.1 bits (157), Expect = 4e-12
Identities = 38/87 (43%), Positives = 47/87 (53%)
Frame = +2
Query: 18 RWNPPPEDFIKINVDGSSFGNPDNAGFGGLLRNNRGNWIHGFSGSCGRATNLFAELSAIW 77
RW P K+N DGSS GN NAG GGLLR+ N+I GFS A + L AI
Sbjct: 2 RWLKPVHGRFKLNFDGSSLGNRGNAGGGGLLRDGSSNFIFGFSIFLAVAQIMKLSLCAIL 181
Query: 78 KGLQLAWDLGYRSIIMESDSQVALDLI 104
+GL + LGY I +E DS + + I
Sbjct: 182 EGLLVCKPLGYDGIGIECDSNIVVSWI 262
>BP036240
Length = 567
Score = 59.3 bits (142), Expect = 2e-10
Identities = 39/110 (35%), Positives = 62/110 (55%), Gaps = 3/110 (2%)
Frame = -3
Query: 19 WNPPPEDFIKINVDGSSFGNPDNAGFGGLLRNNRGNWIHGFSGSCGRAT--NLF-AELSA 75
W P E + K +VDG+ A GG+LR++R +WI GF + G T N+F ELSA
Sbjct: 562 WTAPDEGWTKFDVDGA-LSRDLLASCGGVLRDSRRHWIKGFCRNMGDLTYHNVFLVELSA 386
Query: 76 IWKGLQLAWDLGYRSIIMESDSQVALDLILDTKQKDFHPHASLLSLIRKL 125
I +++A + + +I+ESD+ A+ L L ++ HP SL + I ++
Sbjct: 385 IQSAVEIALSMDLQHVIVESDALEAIHL-LSSENVHAHPFGSLAASILRM 239
>BP080173
Length = 382
Score = 58.9 bits (141), Expect = 3e-10
Identities = 37/112 (33%), Positives = 59/112 (52%)
Frame = -2
Query: 17 VRWNPPPEDFIKINVDGSSFGNPDNAGFGGLLRNNRGNWIHGFSGSCGRATNLFAELSAI 76
VRW P E +IKIN+DGS + + A GG++ ++ G +I G S G AEL I
Sbjct: 351 VRWPVPMEGWIKINIDGSFAPSSNCAACGGVMWDHEGTFITGASVRLGCYFINHAELWGI 172
Query: 77 WKGLQLAWDLGYRSIIMESDSQVALDLILDTKQKDFHPHASLLSLIRKLSSL 128
+ G ++A G++ I++ESDS + L+ + P L++ + SL
Sbjct: 171 FHGAKIAQARGFKKILVESDSSFVVSLV----NQGCSPSKPATPLVKDIKSL 28
>BI418523
Length = 496
Score = 58.2 bits (139), Expect = 5e-10
Identities = 31/87 (35%), Positives = 49/87 (55%)
Frame = +1
Query: 19 WNPPPEDFIKINVDGSSFGNPDNAGFGGLLRNNRGNWIHGFSGSCGRATNLFAELSAIWK 78
W+PP FIK NVDG+S GNP +G GG+ R+ + FS + G A++ +I
Sbjct: 187 WSPPSAGFIKCNVDGASQGNPGPSGVGGVFRDANRKILGYFSLNSGNGWAYEAKVRSILN 366
Query: 79 GLQLAWDLGYRSIIMESDSQVALDLIL 105
L ++I++ESDS VA++ ++
Sbjct: 367 ALVFIQKFLLKNILIESDSTVAVEWVM 447
>BP056414
Length = 606
Score = 57.0 bits (136), Expect = 1e-09
Identities = 34/105 (32%), Positives = 56/105 (52%), Gaps = 5/105 (4%)
Frame = -3
Query: 19 WNPPPEDFIKINVDGSSFGNPDNAGFGGLLRNNRGNWIHGFSGSCGRA---TNLFAELSA 75
W PPE + K+NVDG+ G+ A GG++R+ G+W GF+ S G F EL A
Sbjct: 562 WTRPPEGWFKVNVDGALSGDL-QASCGGVVRDAAGSWSKGFARSLGVLRWHXAFFVELMA 386
Query: 76 IWKGLQ--LAWDLGYRSIIMESDSQVALDLILDTKQKDFHPHASL 118
+ + ++WD+ +I+ESDSQ ++L+ + H + +
Sbjct: 385 VQTAVDFIMSWDI--PQVIIESDSQQVVELLQNFNASSPHQYGEI 257
>TC19596
Length = 499
Score = 54.7 bits (130), Expect = 5e-09
Identities = 40/110 (36%), Positives = 57/110 (51%), Gaps = 3/110 (2%)
Frame = +1
Query: 28 KINVDGSSFGNPDNAGFGGLLRNNRGNWIHGF-SGSCGRATNLFAELSAIWKGLQLAWDL 86
+++ DG+ + D G GG++R+ G WI GF +GS G L AE++A+ GL L W+
Sbjct: 4 RMDSDGAFKHDDDRMGMGGIVRDAHGAWISGFYAGSLG-GDALRAEIAALKHGLTLLWNA 180
Query: 87 GYRSIIMESDSQVALDLI--LDTKQKDFHPHASLLSLIRKLSSLPWVVYL 134
R E D LD++ L+ + FH AS L IR L W V L
Sbjct: 181 HVRRATCEVD---CLDIVEALENDRYQFHALASELLDIRLLLDRDWTVTL 321
>AV407204
Length = 432
Score = 52.0 bits (123), Expect = 4e-08
Identities = 29/82 (35%), Positives = 41/82 (49%), Gaps = 2/82 (2%)
Frame = -2
Query: 55 WIHGFSGSCGRATN--LFAELSAIWKGLQLAWDLGYRSIIMESDSQVALDLILDTKQKDF 112
W++GF A + AE A+ GL+LAWD G R ++ SD + +D I D + F
Sbjct: 428 WVNGFISQYLEAASCSFLAEALALRDGLRLAWDNGTRKLVCNSDCKELIDAIADPSRASF 249
Query: 113 HPHASLLSLIRKLSSLPWVVYL 134
H H +L I L + W V L
Sbjct: 248 HAHGWVLREIHALMTRNWRVEL 183
>TC13560
Length = 571
Score = 51.6 bits (122), Expect = 5e-08
Identities = 32/92 (34%), Positives = 46/92 (49%), Gaps = 3/92 (3%)
Frame = +2
Query: 19 WNPPPEDFIKINVDGSSFGNPDNAGFGGLLRNNRGNWIHGFS---GSCGRATNLFAELSA 75
W P ++KINVDG+ N A G+LRN G+W+ GF G+ A EL A
Sbjct: 146 WIKPDAGWVKINVDGA-VSNCLQASCRGVLRNADGSWLKGFRWNFGTFSSANVFLTELMA 322
Query: 76 IWKGLQLAWDLGYRSIIMESDSQVALDLILDT 107
+ ++ A L +I+ESDS +D + T
Sbjct: 323 VKTAVEAAMSLDLARVIVESDSLEVVDFLHST 418
>BP069214
Length = 545
Score = 50.4 bits (119), Expect = 1e-07
Identities = 30/72 (41%), Positives = 41/72 (56%)
Frame = -2
Query: 61 GSCGRATNLFAELSAIWKGLQLAWDLGYRSIIMESDSQVALDLILDTKQKDFHPHASLLS 120
G+ TN+ EL A+W GL L WD G+R +I +SDS AL L+L H +A+L+
Sbjct: 421 GTL*EITNM--ELLAMWHGLDLVWDRGFRQVICQSDSTEALALVLGVPPPR-HRYAALIW 251
Query: 121 LIRKLSSLPWVV 132
I+ L S W V
Sbjct: 250 SIKDLLSREWEV 215
>BI417841
Length = 617
Score = 46.6 bits (109), Expect = 1e-06
Identities = 35/100 (35%), Positives = 55/100 (55%), Gaps = 1/100 (1%)
Frame = +1
Query: 29 INVDGSSFGNPDNAGFGGLLRNNRGNWIHGFSGSCGRATNLFAELSAIWKGLQLAWDLGY 88
+ DGSS GNP +AG G +LR G+ ++ G G TN AE + GL+ A + GY
Sbjct: 142 LEFDGSSKGNPGSAGAGAVLRAEDGSKVYLREG-VGNQTNNQAEYRGLILGLKHAHEQGY 318
Query: 89 RSIIMESDSQVALDLILDTKQKDFHPH-ASLLSLIRKLSS 127
+ I ++ DSQ+ + + K +P+ ASL + ++L S
Sbjct: 319 QHINVKGDSQLVCKQV-EGSWKARNPNIASLCNEAKELKS 435
>TC11605
Length = 546
Score = 46.2 bits (108), Expect = 2e-06
Identities = 25/65 (38%), Positives = 35/65 (53%)
Frame = +3
Query: 19 WNPPPEDFIKINVDGSSFGNPDNAGFGGLLRNNRGNWIHGFSGSCGRATNLFAELSAIWK 78
W PP + +K NVDGS+ G+P +G GG+LRN + FS G AE+ AI
Sbjct: 348 WCPPEANILKFNVDGSARGSPGISGAGGILRNAESAVLGRFSKPLGVLCAYQAEVKAILL 527
Query: 79 GLQLA 83
L+ +
Sbjct: 528 ALKFS 542
>AV780445
Length = 504
Score = 43.1 bits (100), Expect = 2e-05
Identities = 17/39 (43%), Positives = 25/39 (63%)
Frame = +1
Query: 18 RWNPPPEDFIKINVDGSSFGNPDNAGFGGLLRNNRGNWI 56
RW PP + +K+NVDGS G GGL+R+++G W+
Sbjct: 388 RWQPPRQGTVKLNVDGSGREQDRCMGAGGLIRDDQGKWL 504
>BP033684
Length = 557
Score = 43.1 bits (100), Expect = 2e-05
Identities = 22/55 (40%), Positives = 30/55 (54%)
Frame = -1
Query: 19 WNPPPEDFIKINVDGSSFGNPDNAGFGGLLRNNRGNWIHGFSGSCGRATNLFAEL 73
W PP +K NVDG+S GNP +G GG+L + R + FS + G AE+
Sbjct: 287 WTPPSRGILKFNVDGASQGNPGPSGVGGVLCDYRSMVLGFFSINMGHGWAYEAEV 123
>TC11350 similar to UP|Q9LJP7 (Q9LJP7) Genomic DNA, chromosome 3, P1 clone:
MIG10, partial (18%)
Length = 1118
Score = 43.1 bits (100), Expect = 2e-05
Identities = 27/84 (32%), Positives = 41/84 (48%)
Frame = -3
Query: 19 WNPPPEDFIKINVDGSSFGNPDNAGFGGLLRNNRGNWIHGFSGSCGRATNLFAELSAIWK 78
W PPP K+N D S + G ++R+N G I G + + + +E A+ +
Sbjct: 795 WRPPPLGVYKLNSDASWKAPSPSCTVGVVIRDNLGLLISGTASRPLAPSPIVSEALALRE 616
Query: 79 GLQLAWDLGYRSIIMESDSQVALD 102
GL LA LG I+ ESD Q ++
Sbjct: 615 GLILARSLGLDRILSESDCQPLIE 544
>BP053073
Length = 456
Score = 40.8 bits (94), Expect = 8e-05
Identities = 26/74 (35%), Positives = 39/74 (52%)
Frame = -2
Query: 59 FSGSCGRATNLFAELSAIWKGLQLAWDLGYRSIIMESDSQVALDLILDTKQKDFHPHASL 118
FS G + L EL + GLQL W G R +++ +DSQ+AL L+ FH +A++
Sbjct: 455 FSIFVGISEILKXELLXMLYGLQL*WSKGVRRMLVFTDSQLALSLV-QLGCDRFHQYAAI 279
Query: 119 LSLIRKLSSLPWVV 132
+ +I L W V
Sbjct: 278 VGMIHDLLRFDWQV 237
>AV780577
Length = 486
Score = 40.0 bits (92), Expect = 1e-04
Identities = 29/97 (29%), Positives = 49/97 (49%), Gaps = 1/97 (1%)
Frame = +2
Query: 4 ALGPTNKGHVICQVRWNPPPEDFIKINVDGSSFGNPDNAGFGGLLRNNRGNWIHGFSGSC 63
A GP KG ++ W PPP +KIN+ D GFG ++R+ G + +
Sbjct: 194 ANGPV-KGLILGPSTWWPPPHGKLKINIVDVVVPQVDMMGFGFVMRDANGLVLVSGASKH 370
Query: 64 GRATNL-FAELSAIWKGLQLAWDLGYRSIIMESDSQV 99
R+ ++ E A +Q+++DLG+R ++ S+S V
Sbjct: 371 RRSLSVAMGEALA*RWAMQVSFDLGHRVLMFVSNSMV 481
>TC10933 similar to UP|Q9LNS0 (Q9LNS0) F1L3.4, partial (6%)
Length = 596
Score = 33.1 bits (74), Expect = 0.017
Identities = 21/71 (29%), Positives = 27/71 (37%), Gaps = 28/71 (39%)
Frame = +2
Query: 18 RWNPPPEDFIKINVDGS----------------------------SFGNPDNAGFGGLLR 49
RW PP ++K+N DGS +F DN F LL+
Sbjct: 104 RWTPPSVGWVKLNTDGSFTRGSSLMGCGGLC*K**SDLFM*P*HNNFSF*DNWLFDSLLQ 283
Query: 50 NNRGNWIHGFS 60
+ GNW GFS
Sbjct: 284 DRHGNWRAGFS 316
>BP052637
Length = 473
Score = 32.7 bits (73), Expect = 0.022
Identities = 18/53 (33%), Positives = 28/53 (51%), Gaps = 3/53 (5%)
Frame = -2
Query: 46 GLLRNNRGNWIHGFSGSCGRATNLFAELSAIWKGLQLAWDLG---YRSIIMES 95
G+LR+ W+ GF+ + G A+L A+ + LQ W L YR ++M S
Sbjct: 367 GVLRDKVXRWVSGFAMAFGTGDAFMAKLQALHESLQHVWGLRFVFYR*MLMVS 209
>TC9793
Length = 568
Score = 32.3 bits (72), Expect = 0.029
Identities = 30/97 (30%), Positives = 48/97 (48%), Gaps = 4/97 (4%)
Frame = +2
Query: 19 WNPPPEDFIKINVDGSSFGNPDNAGFGGLLRNNRGNWIHGFSGSCGRATNL---FAELSA 75
W PPPE IK+N+D G+ + GF + R++ N + + + T + AE A
Sbjct: 62 WEPPPEGIIKVNIDAGWSGS-CSTGFSLVARSH--NAVMLVAATHLEETQIEPTLAEALA 232
Query: 76 IWKGLQLAWDLGYRSIIMESDSQVALD-LILDTKQKD 111
+ L + ++ II+ES S V L+ L TK+ D
Sbjct: 233 LRWCLSMIKEMEMERIIVESYSLVVLNTLHQKTKRPD 343
>BP062431
Length = 482
Score = 32.0 bits (71), Expect = 0.038
Identities = 24/72 (33%), Positives = 34/72 (46%)
Frame = -1
Query: 34 SSFGNPDNAGFGGLLRNNRGNWIHGFSGSCGRATNLFAELSAIWKGLQLAWDLGYRSIIM 93
SS G+ NA GGLLR+ ++I GFS A + + L L L Y I +
Sbjct: 482 SSLGHRGNAVGGGLLRHGSSHFIFGFSNFLA-----VARIMKLSYALFLKAYLCYAGIGI 318
Query: 94 ESDSQVALDLIL 105
E DS + + I+
Sbjct: 317 ECDSNIVISWIV 282
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.322 0.140 0.453
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,149,270
Number of Sequences: 28460
Number of extensions: 43870
Number of successful extensions: 214
Number of sequences better than 10.0: 56
Number of HSP's better than 10.0 without gapping: 208
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 209
length of query: 136
length of database: 4,897,600
effective HSP length: 81
effective length of query: 55
effective length of database: 2,592,340
effective search space: 142578700
effective search space used: 142578700
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 50 (23.9 bits)
Medicago: description of AC121245.12