BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC121242.1 + phase: 0 /pseudo
(403 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP065719 50 9e-07
AU251673 32 0.25
AV419203 30 0.94
TC13744 weakly similar to UP|Q39126 (Q39126) HYP1 protein (T4P13... 29 1.6
TC14378 similar to UP|O23254 (O23254) Serine hydroxymethyltransf... 28 2.1
TC14312 similar to UP|Q8J174 (Q8J174) RfeF, partial (7%) 28 2.7
BP063804 27 4.7
TC15321 similar to UP|BAD09664 (BAD09664) BHLH protein family-li... 27 6.1
AV407955 27 6.1
TC9521 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, part... 27 7.9
AV409031 27 7.9
>BP065719
Length = 567
Score = 49.7 bits (117), Expect = 9e-07
Identities = 34/119 (28%), Positives = 55/119 (45%)
Frame = +3
Query: 45 QFSGSPTEDPNLHISSFLRLSGTIKENQEAVRLHLFPFSLRDRASAWFHSLEVGSITSWD 104
+FSG E HI+ + +G + N E +++ FP SL A WF +L S+ +W
Sbjct: 195 KFSGDSGESTVEHIARYQIEAGDLAIN-ENLKMKYFPSSLTKNAFTWFTTLAPRSVHTWA 371
Query: 105 DMRRAFLARFFPPSKTAKLRDQITRFNQKDGESLYEAWERFKEMLRLCPHHGLEKWLIV 163
+ R F +FF +D + +K ES+ + RF+ + C H E L+V
Sbjct: 372 QLERIFHEQFFRGECKVSXKD-LASVKRKPAESIDDYLNRFRMLKSRCFTHVSEHELVV 545
>AU251673
Length = 413
Score = 31.6 bits (70), Expect = 0.25
Identities = 25/88 (28%), Positives = 36/88 (40%), Gaps = 5/88 (5%)
Frame = +2
Query: 77 LHLFPFSLRDRASAWFHSLE----VGSIT-SWDDMRRAFLARFFPPSKTAKLRDQITRFN 131
+ L F L A W++ L VGS +W D F+ RF P S R
Sbjct: 23 VELASFQLEGVARDWYNVLTRAKPVGSPPWTWADFSAEFMNRFLPQSVRDGFVRDFERLE 202
Query: 132 QKDGESLYEAWERFKEMLRLCPHHGLEK 159
Q +G ++ E F + R P+ LE+
Sbjct: 203 QAEGMTVSEYSAHFTHLSRYVPYPLLEE 286
>AV419203
Length = 393
Score = 29.6 bits (65), Expect = 0.94
Identities = 23/74 (31%), Positives = 34/74 (45%), Gaps = 2/74 (2%)
Frame = +1
Query: 195 YALIEDMAQNHYQWTNERAVTTPTPFKKEAGIYEVSEYNHLAAK--VEALTQKIEKLNVK 252
Y ++ DM + W E A ++A Y+ Y A + VEAL Q++ K +K
Sbjct: 55 YGVLVDMIRVP-DWAFEAAGQETRVMAQDAATYQPGLYLTPAQREAVEALIQELPKFRLK 231
Query: 253 AVPPSSASPPCEVC 266
AVP + C VC
Sbjct: 232 AVPTDCSE--CPVC 267
>TC13744 weakly similar to UP|Q39126 (Q39126) HYP1 protein (T4P13.21
protein), partial (26%)
Length = 744
Score = 28.9 bits (63), Expect = 1.6
Identities = 16/39 (41%), Positives = 21/39 (53%)
Frame = +3
Query: 79 LFPFSLRDRASAWFHSLEVGSITSWDDMRRAFLARFFPP 117
LF L + ASAW SL GS+ S+ + + L FPP
Sbjct: 627 LFVVKLSE*ASAWAFSLGNGSVNSFWKLLQFLLIDVFPP 743
>TC14378 similar to UP|O23254 (O23254) Serine hydroxymethyltransferase
(Serine methylase) (Glycine hydroxymethyltransferase)
(SHMT) , partial (68%)
Length = 1023
Score = 28.5 bits (62), Expect = 2.1
Identities = 26/91 (28%), Positives = 34/91 (36%), Gaps = 15/91 (16%)
Frame = +1
Query: 306 PQGSYGQAAPPGYTNNQRV--------AQKSSLEILLENCIANQNKNLQELKNQTGFLND 357
P S +APP TN R A SS + +A ++ L+N G L
Sbjct: 196 PSPSSKLSAPPSPTNTPRACPVTATTAATSSSTRSRISAALARFKPSISTLQN--GALTS 369
Query: 358 S-------LSPHTPKCLRPKSPKWPNKWPPL 381
S SPHTP C + W PP+
Sbjct: 370 SPTPAHRPTSPHTPPCSTLTTASWGWISPPV 462
>TC14312 similar to UP|Q8J174 (Q8J174) RfeF, partial (7%)
Length = 622
Score = 28.1 bits (61), Expect = 2.7
Identities = 13/19 (68%), Positives = 14/19 (73%)
Frame = +1
Query: 303 YKNPQGSYGQAAPPGYTNN 321
Y PQGSYGQ APP Y N+
Sbjct: 199 YGAPQGSYGQ-APPSYGNS 252
>BP063804
Length = 511
Score = 27.3 bits (59), Expect = 4.7
Identities = 11/22 (50%), Positives = 13/22 (59%)
Frame = -1
Query: 245 KIEKLNVKAVPPSSASPPCEVC 266
K E ++ PPSSA PPC C
Sbjct: 175 KPEDHSLSPAPPSSAPPPCSSC 110
>TC15321 similar to UP|BAD09664 (BAD09664) BHLH protein family-like, partial
(23%)
Length = 1593
Score = 26.9 bits (58), Expect = 6.1
Identities = 14/30 (46%), Positives = 17/30 (56%), Gaps = 2/30 (6%)
Frame = -1
Query: 249 LNVKAVPPSSASPPCEVCG--ITGHIGVDC 276
L + VPPS+ S C CG + GH VDC
Sbjct: 1002 LEMLIVPPSTVSSSCWFCGSLVAGH-SVDC 916
>AV407955
Length = 402
Score = 26.9 bits (58), Expect = 6.1
Identities = 20/68 (29%), Positives = 31/68 (45%)
Frame = -2
Query: 48 GSPTEDPNLHISSFLRLSGTIKENQEAVRLHLFPFSLRDRASAWFHSLEVGSITSWDDMR 107
GS E P + + G ++ ++ LFP S+RDR+ + VG S ++R
Sbjct: 221 GSSKEPPPAPLEGLRPVFGNLRMSR*-----LFP*SVRDRSGDFSWDA*VGETFSEGELR 57
Query: 108 RAFLARFF 115
FLA F
Sbjct: 56 VEFLAELF 33
>TC9521 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, partial (56%)
Length = 598
Score = 26.6 bits (57), Expect = 7.9
Identities = 12/28 (42%), Positives = 13/28 (45%)
Frame = +1
Query: 256 PSSASPPCEVCGITGHIGVDCQLGSAAN 283
P S C CGI GH DC+ G N
Sbjct: 370 PPPGSGRCFNCGIDGHWARDCKAGDWKN 453
>AV409031
Length = 343
Score = 26.6 bits (57), Expect = 7.9
Identities = 13/47 (27%), Positives = 24/47 (50%)
Frame = +2
Query: 276 CQLGSAANIEQLNYAQYNQGMRPNQNFYKNPQGSYGQAAPPGYTNNQ 322
CQLG + ++ + P+ ++ PQGS ++A PG+ + Q
Sbjct: 80 CQLGRRTQ*RAVGHS----AIPPHLRHHEPPQGSPAESAQPGFLSTQ 208
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.315 0.131 0.395
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,620,657
Number of Sequences: 28460
Number of extensions: 111568
Number of successful extensions: 675
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 668
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 675
length of query: 403
length of database: 4,897,600
effective HSP length: 92
effective length of query: 311
effective length of database: 2,279,280
effective search space: 708856080
effective search space used: 708856080
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 56 (26.2 bits)
Medicago: description of AC121242.1