BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC121240.11 + phase: 0 /pseudo
(153 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC7796 homologue to gb|AF036495.1|AF036495 Hamamelis virginiana ... 246 1e-66
TC7805 homologue to PIR|PQ0772|PQ0772 4-coumarate-CoA ligase (c... 57 1e-09
TC15031 weakly similar to UP|Q69107 (Q69107) LAT protein, partia... 31 0.11
TC11782 similar to UP|Q9LUV6 (Q9LUV6) Genomic DNA, chromosome 3,... 28 0.70
TC10315 similar to UP|P93166 (P93166) SCOF-1, partial (42%) 27 1.2
AV779148 27 1.2
TC10650 27 1.6
AV406612 27 1.6
TC12500 similar to GB|AAH45354.1|28279536|BC045354 FNBP4 protein... 27 2.0
TC16419 26 2.7
TC17410 26 3.5
BP067043 26 3.5
TC18850 similar to UP|Q41414 (Q41414) Epoxide hydrolase , parti... 26 3.5
TC15655 similar to UP|Q8LE99 (Q8LE99) Transketolase-like protein... 26 3.5
TC8053 similar to UP|Q94A20 (Q94A20) AT4g15470/dl3775w, partial ... 26 3.5
AV421787 25 4.5
TC12427 similar to UP|Q9LK21 (Q9LK21) Genomic DNA, chromosome 3,... 25 4.5
BP050025 25 4.5
NP817340 galactomannan galactosyltransferase [Lotus japonicus] 25 4.5
CB829112 25 5.9
>TC7796 homologue to gb|AF036495.1|AF036495 Hamamelis virginiana large
subunit 26S ribosomal RNA gene, partial sequence,
complete
Length = 6006
Score = 246 bits (629), Expect = 1e-66
Identities = 118/126 (93%), Positives = 120/126 (94%)
Frame = -2
Query: 1 MIGIADIEGSKSNVAMNAWMPQASYPCGNFSDTSSFKFRRSKGSIGHAFTVGIRTGNQNQ 60
MIG ADIEGSKSNVAMNAW+PQASYPCGNFSDTSSFKFRRSKGSIGHAFTV IRTGNQNQ
Sbjct: 5486 MIGRADIEGSKSNVAMNAWLPQASYPCGNFSDTSSFKFRRSKGSIGHAFTVRIRTGNQNQ 5307
Query: 61 TSFYPFVPHEISVLVKLILGHLRYLLTDVPPQPNSPPDNVFRPDRPTKVGLGSKKRGSAP 120
TSFYPFVPHEISVLV+LILGHLRYLLTDVPPQPNSPPDNVFRPDRP K L SKKRGSAP
Sbjct: 5306 TSFYPFVPHEISVLVELILGHLRYLLTDVPPQPNSPPDNVFRPDRPAKASLRSKKRGSAP 5127
Query: 121 PPIHGI 126
PPIHGI
Sbjct: 5126 PPIHGI 5109
Score = 60.5 bits (145), Expect = 1e-10
Identities = 33/73 (45%), Positives = 40/73 (54%), Gaps = 10/73 (13%)
Frame = -3
Query: 91 PQPNSPPDNVFRPDRPTKVGLGSKKRGSAP---PPIHGI-------ITDPFCRLPLPTLF 140
P P PP RP + +++ + PP + +TDPFCRLPLPTLF
Sbjct: 4654 PFPIRPPTGTRRPALAAEAARAVRRQPTGSELGPPCPALRANPFPEVTDPFCRLPLPTLF 4475
Query: 141 HRPEAVHLGDLMR 153
H PEAVHLGDLMR
Sbjct: 4474 H*PEAVHLGDLMR 4436
>TC7805 homologue to PIR|PQ0772|PQ0772 4-coumarate-CoA ligase (clone
GM4CL1B) - soybean (fragment) {Glycine
max;} , partial (41%)
Length = 965
Score = 57.0 bits (136), Expect = 1e-09
Identities = 25/33 (75%), Positives = 26/33 (78%)
Frame = -1
Query: 85 LLTDVPPQPNSPPDNVFRPDRPTKVGLGSKKRG 117
L +PPQPNSPPDNVFRPDRP K L SKKRG
Sbjct: 101 LSISLPPQPNSPPDNVFRPDRPAKASLRSKKRG 3
>TC15031 weakly similar to UP|Q69107 (Q69107) LAT protein, partial (27%)
Length = 997
Score = 30.8 bits (68), Expect = 0.11
Identities = 13/42 (30%), Positives = 19/42 (44%)
Frame = +2
Query: 58 QNQTSFYPFVPHEISVLVKLILGHLRYLLTDVPPQPNSPPDN 99
+ Q SFYP P + + + YL PP P PP++
Sbjct: 176 REQKSFYPRCPPPAKITTTALYASMLYLTLSPPPLPPPPPES 301
>TC11782 similar to UP|Q9LUV6 (Q9LUV6) Genomic DNA, chromosome 3, P1 clone:
MCB17, partial (12%)
Length = 468
Score = 28.1 bits (61), Expect = 0.70
Identities = 12/35 (34%), Positives = 16/35 (45%)
Frame = +2
Query: 91 PQPNSPPDNVFRPDRPTKVGLGSKKRGSAPPPIHG 125
P+PN P D P + +RG PPP +G
Sbjct: 263 PKPNPNPSPKHPTDHPPLIPFPFPERGPPPPPAYG 367
>TC10315 similar to UP|P93166 (P93166) SCOF-1, partial (42%)
Length = 592
Score = 27.3 bits (59), Expect = 1.2
Identities = 27/93 (29%), Positives = 33/93 (35%), Gaps = 27/93 (29%)
Frame = +3
Query: 57 NQNQTSFYPFVPHEISVLV------KLILGHLRYLLTDVPPQP----------------- 93
NQN+TS + F S L KLI L T PP P
Sbjct: 51 NQNKTSLFQFQVFTFSSLSFRIRSQKLIK*WLWKPSTHPPPPPHPSRSTTTQPSVTRRSR 230
Query: 94 ----NSPPDNVFRPDRPTKVGLGSKKRGSAPPP 122
N+P D V P + T + S +APPP
Sbjct: 231 GQNGNAPSDLVHAPRKSTSLSASSCSLAAAPPP 329
>AV779148
Length = 537
Score = 27.3 bits (59), Expect = 1.2
Identities = 10/19 (52%), Positives = 14/19 (73%)
Frame = +3
Query: 85 LLTDVPPQPNSPPDNVFRP 103
+++DVPPQP P + V RP
Sbjct: 81 VVSDVPPQPEKPAEKVPRP 137
>TC10650
Length = 636
Score = 26.9 bits (58), Expect = 1.6
Identities = 12/29 (41%), Positives = 17/29 (58%), Gaps = 1/29 (3%)
Frame = +3
Query: 77 LILGHLRYLLTDV-PPQPNSPPDNVFRPD 104
L+L L + L++ PP P PP N + PD
Sbjct: 453 LLLNFLFFFLSEFKPPNPPEPPANPYHPD 539
>AV406612
Length = 313
Score = 26.9 bits (58), Expect = 1.6
Identities = 12/39 (30%), Positives = 19/39 (47%)
Frame = +1
Query: 91 PQPNSPPDNVFRPDRPTKVGLGSKKRGSAPPPIHGIITD 129
P NSPP++ P +P + +APPP++ D
Sbjct: 40 PFLNSPPNHSLIPPQPMDTAAAIPEPEAAPPPLYDSAID 156
>TC12500 similar to GB|AAH45354.1|28279536|BC045354 FNBP4 protein {Danio
rerio;} , partial (5%)
Length = 678
Score = 26.6 bits (57), Expect = 2.0
Identities = 18/63 (28%), Positives = 27/63 (42%)
Frame = +1
Query: 81 HLRYLLTDVPPQPNSPPDNVFRPDRPTKVGLGSKKRGSAPPPIHGIITDPFCRLPLPTLF 140
HL L T PP + T+ L S + +PPP +G+ T P LP+ ++
Sbjct: 421 HLTSLTTPSPPSSRTTI---------TQTPLPSPPQPPSPPPKYGVATIPPPPLPMSSID 573
Query: 141 HRP 143
P
Sbjct: 574 RTP 582
>TC16419
Length = 532
Score = 26.2 bits (56), Expect = 2.7
Identities = 11/24 (45%), Positives = 15/24 (61%)
Frame = -3
Query: 93 PNSPPDNVFRPDRPTKVGLGSKKR 116
P+SPP N + + +GLG KKR
Sbjct: 332 PSSPPRNCLQVSQRCLLGLGEKKR 261
>TC17410
Length = 420
Score = 25.8 bits (55), Expect = 3.5
Identities = 13/28 (46%), Positives = 14/28 (49%)
Frame = +2
Query: 88 DVPPQPNSPPDNVFRPDRPTKVGLGSKK 115
D P P PP N+F D VG GS K
Sbjct: 11 DEPNNPPPPPLNLFAGDDSVGVGSGSVK 94
>BP067043
Length = 530
Score = 25.8 bits (55), Expect = 3.5
Identities = 15/35 (42%), Positives = 19/35 (53%), Gaps = 5/35 (14%)
Frame = +1
Query: 91 PQPNSPPDNVFRPDRPT-----KVGLGSKKRGSAP 120
P P PPDN P+ PT VG+GS+K +P
Sbjct: 310 PNPPEPPDNPCHPN-PT*PINLTVGIGSEKLRFSP 411
>TC18850 similar to UP|Q41414 (Q41414) Epoxide hydrolase , partial (22%)
Length = 474
Score = 25.8 bits (55), Expect = 3.5
Identities = 12/25 (48%), Positives = 12/25 (48%)
Frame = -3
Query: 124 HGIITDPFCRLPLPTLFHRPEAVHL 148
HG D PLPT H PE HL
Sbjct: 175 HGAFLDGGSVPPLPTQPHHPEVAHL 101
>TC15655 similar to UP|Q8LE99 (Q8LE99) Transketolase-like protein, partial
(17%)
Length = 575
Score = 25.8 bits (55), Expect = 3.5
Identities = 11/30 (36%), Positives = 13/30 (42%)
Frame = +1
Query: 118 SAPPPIHGIITDPFCRLPLPTLFHRPEAVH 147
S P P H + P PLP RP +H
Sbjct: 64 SLPSPPHAPLFPPLLHAPLPPRLLRPPPIH 153
>TC8053 similar to UP|Q94A20 (Q94A20) AT4g15470/dl3775w, partial (82%)
Length = 1109
Score = 25.8 bits (55), Expect = 3.5
Identities = 9/16 (56%), Positives = 12/16 (74%)
Frame = -3
Query: 44 SIGHAFTVGIRTGNQN 59
S+ H FT+G+ TGN N
Sbjct: 480 SLKHNFTIGVSTGNSN 433
>AV421787
Length = 250
Score = 25.4 bits (54), Expect = 4.5
Identities = 13/43 (30%), Positives = 16/43 (36%)
Frame = +2
Query: 87 TDVPPQPNSPPDNVFRPDRPTKVGLGSKKRGSAPPPIHGIITD 129
T P P+SPP + + P S SA P TD
Sbjct: 98 TQPKPPPHSPPTSAYGTPSPPPPSAASSSTPSAAAPAPATTTD 226
>TC12427 similar to UP|Q9LK21 (Q9LK21) Genomic DNA, chromosome 3, TAC
clone:K17E12, partial (20%)
Length = 597
Score = 25.4 bits (54), Expect = 4.5
Identities = 19/63 (30%), Positives = 23/63 (36%)
Frame = +1
Query: 72 SVLVKLILGHLRYLLTDVPPQPNSPPDNVFRPDRPTKVGLGSKKRGSAPPPIHGIITDPF 131
SV K +L H PP+ + P RP+ S G A IH DPF
Sbjct: 253 SVFFKFLLKHRLQNRIQAPPETSDPFGVTSRPEESVAAANPSFTDGVATKDIH---IDPF 423
Query: 132 CRL 134
L
Sbjct: 424 TSL 432
>BP050025
Length = 552
Score = 25.4 bits (54), Expect = 4.5
Identities = 15/47 (31%), Positives = 18/47 (37%)
Frame = +1
Query: 76 KLILGHLRYLLTDVPPQPNSPPDNVFRPDRPTKVGLGSKKRGSAPPP 122
K + L PP+P+SP RPT G K G P P
Sbjct: 133 KTVYASLTGFRRTTPPEPDSPDQ------RPTVEVTGHKAHGPVPQP 255
>NP817340 galactomannan galactosyltransferase [Lotus japonicus]
Length = 1314
Score = 25.4 bits (54), Expect = 4.5
Identities = 13/40 (32%), Positives = 21/40 (52%), Gaps = 4/40 (10%)
Frame = -1
Query: 68 PHEISVLVKLILGHLRYLLTDV----PPQPNSPPDNVFRP 103
P + + ++L HL L D+ PPQP+ PP ++ P
Sbjct: 1113 PPPLPKIFHMLLPHLAIPLADLLRVPPPQPSHPPLHLIVP 994
>CB829112
Length = 234
Score = 25.0 bits (53), Expect = 5.9
Identities = 12/30 (40%), Positives = 15/30 (50%)
Frame = +3
Query: 90 PPQPNSPPDNVFRPDRPTKVGLGSKKRGSA 119
PP P PP P P+++ S KR SA
Sbjct: 117 PPPPPPPPATHILPMTPSRMTQMSSKRSSA 206
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.321 0.141 0.442
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,084,280
Number of Sequences: 28460
Number of extensions: 53482
Number of successful extensions: 438
Number of sequences better than 10.0: 50
Number of HSP's better than 10.0 without gapping: 426
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 433
length of query: 153
length of database: 4,897,600
effective HSP length: 82
effective length of query: 71
effective length of database: 2,563,880
effective search space: 182035480
effective search space used: 182035480
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 51 (24.3 bits)
Medicago: description of AC121240.11