BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC121237.9 + phase: 0 /pseudo
(652 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP075318 83 2e-27
BP067009 97 1e-20
BP082878 75 3e-14
TC14751 similar to UP|Q948T9 (Q948T9) Respiratory burst oxidase ... 75 4e-14
TC17085 similar to UP|Q84KK7 (Q84KK7) Respiratory burst oxidase ... 56 2e-08
BE122544 54 8e-08
TC17613 34 0.084
TC16469 homologue to UP|Q9FEZ7 (Q9FEZ7) Ribosomal protein L2, co... 31 0.55
TC8273 UP|BAD11134 (BAD11134) Glutamate-rich protein, complete 30 0.93
TC8488 similar to UP|Q9ZNT1 (Q9ZNT1) NADH-cytochrome B5 reductas... 30 1.2
TC11010 homologue to UP|PPX2_ARATH (P48528) Serine/threonine pro... 30 1.6
TC15365 homologue to GB|AAP78936.1|32306505|BT009668 At5g19990 {... 29 2.1
BP052586 29 2.7
TC17023 homologue to UP|Q9FM19 (Q9FM19) Hypersensitive-induced r... 29 2.7
TC11422 UP|NIA_LOTJA (P39869) Nitrate reductase [NADH] (NR) , c... 28 3.5
BP043309 28 4.6
BP084865 28 4.6
BP053126 28 4.6
BP084568 24 5.2
TC18735 similar to UP|DSX_DROME (P23023) Doublesex protein, part... 28 6.1
>BP075318
Length = 471
Score = 83.2 bits (204), Expect(2) = 2e-27
Identities = 37/46 (80%), Positives = 42/46 (90%)
Frame = -1
Query: 607 KKILFECEGKDVGVMTCGPRKMRHEVARICASGLADNLHFESISFN 652
+K+LFE EGKDVGV+ CGP +RHEVARIC+SGLADNLHFESISFN
Sbjct: 390 RKLLFEREGKDVGVLVCGPSNLRHEVARICSSGLADNLHFESISFN 253
Score = 57.0 bits (136), Expect(2) = 2e-27
Identities = 26/29 (89%), Positives = 28/29 (95%)
Frame = -3
Query: 581 ERELESLPHQSLVQATNVHFGSRPDLKKI 609
ERELESLPHQSLVQAT+ HFG+RPDLKKI
Sbjct: 469 ERELESLPHQSLVQATHAHFGARPDLKKI 383
>BP067009
Length = 496
Score = 96.7 bits (239), Expect = 1e-20
Identities = 45/75 (60%), Positives = 56/75 (74%)
Frame = -2
Query: 577 IYGSERELESLPHQSLVQATNVHFGSRPDLKKILFECEGKDVGVMTCGPRKMRHEVARIC 636
I+ +RELESLP Q Q TNVH+G RPDL++++ E G VGV+ GP KMR EVA IC
Sbjct: 495 IHEIDRELESLPLQQFAQVTNVHYGVRPDLRRLISELNGSTVGVLASGPXKMRQEVAAIC 316
Query: 637 ASGLADNLHFESISF 651
+SG A+NLHFES+SF
Sbjct: 315 SSGSAENLHFESLSF 271
>BP082878
Length = 476
Score = 75.5 bits (184), Expect = 3e-14
Identities = 47/160 (29%), Positives = 87/160 (54%), Gaps = 2/160 (1%)
Frame = -1
Query: 358 VEGPYGPHTAQFLRHEQIVMVSGGSGVTPFISIIRDLIFQSQQQEFQPPKLLLVCIFKNY 417
+EGPYGP + +L+++ +++V+GGSG+TPF+SI+ ++ + + F P ++ LV + K
Sbjct: 476 IEGPYGPASFDYLKYDSLLLVAGGSGITPFLSILAEVDSAASRNRF-PLRIQLVYVIKKA 300
Query: 418 ADLTMLDLMLPISGLKTRISQLQLQIEAYITREKQEPS--KDTQKQIQTIWFKTNLSDCP 475
D ++L + + LK L ++ ++T+E QE ++ ++ + S C
Sbjct: 299 QDFSLLHSISHML-LKESTENFHLNLKLFVTQETQEGMGIRELFHELFKVRTLELNSFCS 123
Query: 476 ISAVLGPNNWLWLGAIITSSFVMFLLFLGIVTRYYIYPIE 515
A GP + W+ AI + FL+FL I+ + I P E
Sbjct: 122 SYAAHGPESPAWMAAIAGFCSLTFLIFL-IMFNHIIIPSE 6
>TC14751 similar to UP|Q948T9 (Q948T9) Respiratory burst oxidase homolog,
partial (34%)
Length = 1160
Score = 74.7 bits (182), Expect = 4e-14
Identities = 46/153 (30%), Positives = 76/153 (49%), Gaps = 21/153 (13%)
Frame = +3
Query: 271 LLSARLLPCDALELNFSKNPSLYYNPTSLVFINVPKVSKLQWHPFTVTSSCNMETNYLSV 330
+L + P + L L+ SK Y+ +F+N VS QWHPF++TS+ +Y+SV
Sbjct: 3 ILKVAVYPGNVLALHVSKPQGFKYHSGQYIFVNCRDVSPFQWHPFSITSAPG--DDYVSV 176
Query: 331 AIKNVGSWSNKLYQE---------------LSSSSLDHLNIS------VEGPYGPHTAQF 369
I+ +G W+++L L + L NI ++GPYG +
Sbjct: 177 HIRTLGDWTSQLKTVFAKACQPASGDQSGLLRADMLQGNNIPRMPKLLIDGPYGAPAQDY 356
Query: 370 LRHEQIVMVSGGSGVTPFISIIRDLIFQSQQQE 402
+E I++V G G TP ISI++D++ +QQ+
Sbjct: 357 KDYEVILLVGLGIGATPLISILKDVLNNMKQQK 455
>TC17085 similar to UP|Q84KK7 (Q84KK7) Respiratory burst oxidase homolog,
partial (11%)
Length = 557
Score = 56.2 bits (134), Expect = 2e-08
Identities = 29/80 (36%), Positives = 43/80 (53%)
Frame = +1
Query: 270 RLLSARLLPCDALELNFSKNPSLYYNPTSLVFINVPKVSKLQWHPFTVTSSCNMETNYLS 329
R+L + P + L L+ SK Y +FIN VS +WHPF++TS+ +YLS
Sbjct: 31 RILKVAVYPGNVLALHMSKPQGFRYKSGQYMFINCAAVSPFEWHPFSITSAPG--DDYLS 204
Query: 330 VAIKNVGSWSNKLYQELSSS 349
V I+ +G W+ L + S S
Sbjct: 205 VHIRTLGDWTRSLRVKFSES 264
>BE122544
Length = 337
Score = 53.9 bits (128), Expect = 8e-08
Identities = 32/106 (30%), Positives = 51/106 (47%), Gaps = 22/106 (20%)
Frame = +2
Query: 300 VFINVPKVSKLQWHPFTVTSSCNMETNYLSVAIKNVGSWSNKLYQELSSSSLDHLN---- 355
+F+N VS +WHPF++TS+ + E +LSV IK +G W+ L + S + L LN
Sbjct: 26 MFVNCAAVSPFEWHPFSITSAPDDE--FLSVHIKILGDWTRSLKAKFSEACLPALNGQSG 199
Query: 356 ------------------ISVEGPYGPHTAQFLRHEQIVMVSGGSG 383
+ V+GPYG + +E +++V G G
Sbjct: 200 LLRAECSXGEYSPSNFPKVLVDGPYGAPAQDYREYEVVLLVGLGIG 337
>TC17613
Length = 591
Score = 33.9 bits (76), Expect = 0.084
Identities = 18/51 (35%), Positives = 28/51 (54%), Gaps = 4/51 (7%)
Frame = +3
Query: 592 LVQATNVHFGSRPDLKKILFECEGK----DVGVMTCGPRKMRHEVARICAS 638
+ ++T + +G RPD K+I K DVGV+ CGP ++ VA+ S
Sbjct: 18 IAKSTVLRYGYRPDSKEIFEAMSEKWGNVDVGVLVCGPPTLQSSVAQAIRS 170
>TC16469 homologue to UP|Q9FEZ7 (Q9FEZ7) Ribosomal protein L2, complete
Length = 1003
Score = 31.2 bits (69), Expect = 0.55
Identities = 16/39 (41%), Positives = 23/39 (58%), Gaps = 1/39 (2%)
Frame = +3
Query: 497 VMFLLFLGIVTRYYIYPI-ENNSGEVYNWTYGVMWYMFS 534
++FLL G+ R Y + I ++ EV+NWT MWY S
Sbjct: 870 ILFLLQFGLELRGYCFEICFPDNYEVFNWTSFYMWYDLS 986
>TC8273 UP|BAD11134 (BAD11134) Glutamate-rich protein, complete
Length = 923
Score = 30.4 bits (67), Expect = 0.93
Identities = 18/64 (28%), Positives = 28/64 (43%), Gaps = 5/64 (7%)
Frame = -3
Query: 496 FVMFLLFLGIVTRYYIYPIENNSGEVYNWTYGVMWYMFSFCSCICICSSV-----VFLWL 550
++ FL FL + ++ + N ++W +G W FCSC C C FLW+
Sbjct: 369 YLWFLCFLCLRLGFFCHFFSLN----FSWGFG-FWSSRCFCSCFCYCFHFWFFGGFFLWV 205
Query: 551 KRLN 554
N
Sbjct: 204 FLFN 193
>TC8488 similar to UP|Q9ZNT1 (Q9ZNT1) NADH-cytochrome B5 reductase ,
partial (90%)
Length = 1216
Score = 30.0 bits (66), Expect = 1.2
Identities = 23/81 (28%), Positives = 40/81 (48%), Gaps = 2/81 (2%)
Frame = +1
Query: 356 ISVEGPYGPHTAQFLRHEQIVMVSGGSGVTPFISIIRDLIFQSQQQEFQPPKLLLVCIFK 415
++V+GP G Q M++GGSG+TP + R ++ + K+ L+
Sbjct: 541 LAVKGPKGRFKYQPNDVRAFGMLAGGSGITPMFQVARAILENPNDR----TKVHLI---- 696
Query: 416 NYADLTMLDLML--PISGLKT 434
YA++T D++L + GL T
Sbjct: 697 -YANVTYEDILLKEELDGLAT 756
>TC11010 homologue to UP|PPX2_ARATH (P48528) Serine/threonine protein
phosphatase PP-X isozyme 2 , partial (70%)
Length = 770
Score = 29.6 bits (65), Expect = 1.6
Identities = 22/63 (34%), Positives = 34/63 (53%), Gaps = 3/63 (4%)
Frame = +1
Query: 102 LLHS*YGPFPITYIPVSITSACTNKMRKFTRMSSILPLVGLTSESSIKYHIWLGQ---LS 158
++ S YG +TY SIT AC ++ TR+S + + L + I+Y +W+G L
Sbjct: 520 MVRSRYGDMALTY---SIT*ACPP*LK--TRLSVCMVVFLLPFQDWIRYELWIGSKRYLM 684
Query: 159 MVL 161
MVL
Sbjct: 685 MVL 693
>TC15365 homologue to GB|AAP78936.1|32306505|BT009668 At5g19990 {Arabidopsis
thaliana;}, partial (87%)
Length = 1385
Score = 29.3 bits (64), Expect = 2.1
Identities = 15/39 (38%), Positives = 21/39 (53%)
Frame = +1
Query: 151 HIWLGQLSMVLFAAHSIGFIIYWAITNQMIEMLEWSKTY 189
H WLGQL +L HS G + N + ++LEWS +
Sbjct: 472 HCWLGQLHTILI-VHSSGCLDLN*FRNTLEKVLEWSGNF 585
>BP052586
Length = 494
Score = 28.9 bits (63), Expect = 2.7
Identities = 18/53 (33%), Positives = 24/53 (44%)
Frame = -2
Query: 207 ITSIPQIRRKMYEVFFYTHHLYILYILFFAIHAGVGSMCVIAPGVFLFLIDRH 259
+TS+P +R E FF L LY+LF G VI F+F + H
Sbjct: 484 VTSVPPHKRDXMETFFLGETLKYLYLLF-------GDSSVIPLDKFVFNTEAH 347
>TC17023 homologue to UP|Q9FM19 (Q9FM19) Hypersensitive-induced response
protein, partial (29%)
Length = 510
Score = 28.9 bits (63), Expect = 2.7
Identities = 22/84 (26%), Positives = 41/84 (48%), Gaps = 13/84 (15%)
Frame = +2
Query: 484 NWLWLGAIITSSF----VMFLL---------FLGIVTRYYIYPIENNSGEVYNWTYGVMW 530
NW +L +++ SF V+F++ F ++T +Y + +V WTY +M
Sbjct: 152 NWCYLPSLLLFSFPMDPVLFVM*QAQFVMDFFRLLITSFYSTQL---LCQVCLWTYMIML 322
Query: 531 YMFSFCSCICICSSVVFLWLKRLN 554
Y+ + S +CS V+ + RL+
Sbjct: 323 YVCVWLSFFEVCSVVLLYLIIRLS 394
>TC11422 UP|NIA_LOTJA (P39869) Nitrate reductase [NADH] (NR) , complete
Length = 2932
Score = 28.5 bits (62), Expect = 3.5
Identities = 29/117 (24%), Positives = 48/117 (40%), Gaps = 21/117 (17%)
Frame = +1
Query: 300 VFINVPKVSKLQWHPFTVTSSCNMETNYLSVAIK-----------NVGSWSNKLYQELSS 348
+F+ KL +T TS + E Y + +K N G+ S L
Sbjct: 2047 IFLCATVDGKLCMRAYTPTSGVD-EVGYFELVVKVYFKGVHPKFPNGGAMSQHLDSLPIG 2223
Query: 349 SSLDHLNISVEGPYG----PHTAQFLRH------EQIVMVSGGSGVTPFISIIRDLI 395
S LD V+GP G FL H +++ M++GG+G+TP + + ++
Sbjct: 2224 SDLD-----VKGPLGHIEYTGRGNFLVHGKHRFAKKLAMLAGGTGITPIYQVAQAIL 2379
>BP043309
Length = 527
Score = 28.1 bits (61), Expect = 4.6
Identities = 30/112 (26%), Positives = 44/112 (38%), Gaps = 4/112 (3%)
Frame = +3
Query: 436 ISQLQLQIEAYITREKQEPSKDTQKQIQTIWFKTNLSDCPISAVLGPNNWLWLGAIITSS 495
IS Q+Q + E++ S ++ + NL+ VL +WL II SS
Sbjct: 132 ISSKQIQKSLSLLLEEEPISLSSRG*VYEASLLRNLTTTSACDVLTILDWLLFHYIICSS 311
Query: 496 FVMFLLFLGIVTRYYI----YPIENNSGEVYNWTYGVMWYMFSFCSCICICS 543
F+ L+L + Y SG + V + FS C C C CS
Sbjct: 312 FLQLQLYLTYPQSQFALKSGYLDHRVSGVRKDEILEVGFCWFSLC-CFCSCS 464
>BP084865
Length = 484
Score = 28.1 bits (61), Expect = 4.6
Identities = 16/49 (32%), Positives = 21/49 (42%)
Frame = -2
Query: 279 CDALELNFSKNPSLYYNPTSLVFINVPKVSKLQWHPFTVTSSCNMETNY 327
C AL L+ + P P F NV V +HP+ V SC + Y
Sbjct: 162 CSALMLSLNFKPPFEPLPIKNNFYNVSSVFGSNYHPYIV*LSC*ISIRY 16
>BP053126
Length = 395
Score = 28.1 bits (61), Expect = 4.6
Identities = 13/51 (25%), Positives = 27/51 (52%)
Frame = -3
Query: 491 IITSSFVMFLLFLGIVTRYYIYPIENNSGEVYNWTYGVMWYMFSFCSCICI 541
++ SF +F FL ++ + + +G Y W + V WY+ + C+C+ +
Sbjct: 156 VLVCSFCIFYGFLYLLKQLCV----ECTGNSY-WLFSVGWYL*TLCACLVV 19
>BP084568
Length = 423
Score = 23.9 bits (50), Expect(2) = 5.2
Identities = 7/21 (33%), Positives = 13/21 (61%)
Frame = -2
Query: 536 CSCICICSSVVFLWLKRLNKL 556
C C+C+C ++ + L+ N L
Sbjct: 77 CVCVCVCVNISMILLQLKNTL 15
Score = 22.3 bits (46), Expect(2) = 5.2
Identities = 5/19 (26%), Positives = 9/19 (47%)
Frame = -1
Query: 524 WTYGVMWYMFSFCSCICIC 542
W + + C C+C+C
Sbjct: 117 WRQNLTYIYIYVCVCVCVC 61
>TC18735 similar to UP|DSX_DROME (P23023) Doublesex protein, partial (4%)
Length = 784
Score = 27.7 bits (60), Expect = 6.1
Identities = 19/58 (32%), Positives = 27/58 (45%), Gaps = 2/58 (3%)
Frame = +2
Query: 296 PTSLVFINVPKVSKLQWHPFTVTSSCNMETNYLSVAIKNVGSWS--NKLYQELSSSSL 351
P L + P S +HP +S N T+ L +A+ N WS NK+ Q + S L
Sbjct: 122 PPHL*WFGFPSFS---FHPSVSSSHSNSSTSTLCLALNNPSLWSGHNKIRQPMRISML 286
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.329 0.140 0.449
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 14,348,726
Number of Sequences: 28460
Number of extensions: 245190
Number of successful extensions: 2262
Number of sequences better than 10.0: 46
Number of HSP's better than 10.0 without gapping: 2165
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2233
length of query: 652
length of database: 4,897,600
effective HSP length: 96
effective length of query: 556
effective length of database: 2,165,440
effective search space: 1203984640
effective search space used: 1203984640
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 58 (26.9 bits)
Medicago: description of AC121237.9