BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC121237.5 + phase: 2 /pseudo
(194 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC19476 similar to UP|Q9LH54 (Q9LH54) Emb|CAB88266.1, partial (15%) 34 0.019
TC13322 similar to UP|Q9ZS13 (Q9ZS13) RNA helicase (Fragment), p... 33 0.025
AW720070 31 0.12
TC19835 similar to UP|Q9ZRW0 (Q9ZRW0) Nucleolar protein (Fragmen... 30 0.21
TC14782 UP|O04360 (O04360) Late nodulin Nlj16, complete 30 0.27
TC14994 similar to UP|O82469 (O82469) Protein phosphatase-2C , p... 29 0.47
TC11310 similar to UP|BAD11331 (BAD11331) BRI1-KD interacting pr... 29 0.47
TC16303 weakly similar to UP|AGA1_YEAST (P32323) A-agglutinin at... 28 1.4
TC9175 weakly similar to UP|INAD_DROME (Q24008) Inactivation-no-... 28 1.4
TC14491 UP|Q43781 (Q43781) Aspartate aminotransferase , complete 27 1.8
AV410716 27 1.8
BP034655 27 2.3
TC18924 homologue to UP|Q94FN3 (Q94FN3) Phosphatidylinositol tra... 27 3.0
BP072343 27 3.0
TC17589 26 3.9
TC12685 weakly similar to UP|Q7QA92 (Q7QA92) AgCP13961 (Fragment... 26 3.9
TC8740 26 3.9
BE122625 26 3.9
TC9825 similar to UP|Q9ZRD8 (Q9ZRD8) GMFP5 (Fragment), partial (... 26 5.2
CB828378 26 5.2
>TC19476 similar to UP|Q9LH54 (Q9LH54) Emb|CAB88266.1, partial (15%)
Length = 545
Score = 33.9 bits (76), Expect = 0.019
Identities = 21/58 (36%), Positives = 33/58 (56%)
Frame = +1
Query: 54 KQVKDELDRLKQAEKKKRRLEKALATSAAIISELEKKKQKKKEEQQRLDEEGAAIAEA 111
++V D+++ L + + +E+ ATS ELEK KQ KE +Q L + A IA+A
Sbjct: 31 ERVSDDIE-LMNTDTLVKEVERVFATSPPDSMELEKAKQMLKEHEQALLDAIARIADA 201
>TC13322 similar to UP|Q9ZS13 (Q9ZS13) RNA helicase (Fragment), partial (7%)
Length = 528
Score = 33.5 bits (75), Expect = 0.025
Identities = 22/59 (37%), Positives = 34/59 (57%), Gaps = 2/59 (3%)
Frame = +3
Query: 50 KTKKKQVKDELDRLKQAEKK--KRRLEKALATSAAIISELEKKKQKKKEEQQRLDEEGA 106
+++KK+ K D+ + EK KR+LE+ + +E EKKK+KKKE+ EE A
Sbjct: 126 QSEKKKKKKNKDKHQNGEKTSPKRKLEELDNQNG---TESEKKKKKKKEKHDNKSEENA 293
>AW720070
Length = 484
Score = 31.2 bits (69), Expect = 0.12
Identities = 18/46 (39%), Positives = 27/46 (58%)
Frame = +2
Query: 51 TKKKQVKDELDRLKQAEKKKRRLEKALATSAAIISELEKKKQKKKE 96
+KKKQ K L K+ +KKK++ +K + EKKK+KKK+
Sbjct: 71 SKKKQ*KVILIAEKKRKKKKKKRKKKKQVKKESSQQQEKKKKKKKK 208
Score = 29.6 bits (65), Expect = 0.36
Identities = 15/39 (38%), Positives = 27/39 (68%)
Frame = +2
Query: 49 RKTKKKQVKDELDRLKQAEKKKRRLEKALATSAAIISEL 87
++ KKKQVK E + +Q +KKK++ + A++T+A + L
Sbjct: 134 KRKKKKQVKKESSQ-QQEKKKKKKKKAAVSTTAPFLHPL 247
>TC19835 similar to UP|Q9ZRW0 (Q9ZRW0) Nucleolar protein (Fragment), partial
(16%)
Length = 550
Score = 30.4 bits (67), Expect = 0.21
Identities = 20/61 (32%), Positives = 36/61 (58%), Gaps = 1/61 (1%)
Frame = +1
Query: 48 ARKTKKKQ-VKDELDRLKQAEKKKRRLEKALATSAAIISELEKKKQKKKEEQQRLDEEGA 106
++K+KKKQ DE D + ++K T+ + + K ++KKK+E+++LD+E A
Sbjct: 106 SKKSKKKQKAADEGDEMA--------VDKPTVTNGDAVED-HKSEKKKKKEKRKLDQEVA 258
Query: 107 A 107
A
Sbjct: 259 A 261
Score = 27.3 bits (59), Expect = 1.8
Identities = 22/69 (31%), Positives = 32/69 (45%), Gaps = 15/69 (21%)
Frame = +1
Query: 45 QQHARKTKKKQVKDELDRLKQAE---------------KKKRRLEKALATSAAIISELEK 89
+ H + KKK+ K +LD+ AE KK R+ + A AA SE +K
Sbjct: 196 EDHKSEKKKKKEKRKLDQEVAAENGTNGNASEPRSVKKKKDRKDDNGEAVEAA--SESKK 369
Query: 90 KKQKKKEEQ 98
KK+ KK +
Sbjct: 370 KKKSKKNAE 396
>TC14782 UP|O04360 (O04360) Late nodulin Nlj16, complete
Length = 1010
Score = 30.0 bits (66), Expect = 0.27
Identities = 15/32 (46%), Positives = 20/32 (61%)
Frame = +3
Query: 64 KQAEKKKRRLEKALATSAAIISELEKKKQKKK 95
KQ K+ LE +LA + + +EKKKQKKK
Sbjct: 354 KQLMDTKKALEDSLAKQEVLSAYVEKKKQKKK 449
>TC14994 similar to UP|O82469 (O82469) Protein phosphatase-2C , partial
(89%)
Length = 1672
Score = 29.3 bits (64), Expect = 0.47
Identities = 17/49 (34%), Positives = 28/49 (56%)
Frame = +3
Query: 67 EKKKRRLEKALATSAAIISELEKKKQKKKEEQQRLDEEGAAIAEAVALH 115
EKKK++L+K +KKK+KKK+++++ + A AE V H
Sbjct: 159 EKKKKKLKKK-----------KKKKKKKKKKKKKKQRKMVAEAEIVCQH 272
Score = 27.7 bits (60), Expect = 1.4
Identities = 11/41 (26%), Positives = 26/41 (62%)
Frame = +3
Query: 46 QHARKTKKKQVKDELDRLKQAEKKKRRLEKALATSAAIISE 86
Q ++ KKK++K + + K+ +KKK++ ++ + A I+ +
Sbjct: 147 QLLQEKKKKKLKKKKKKKKKKKKKKKKKQRKMVAEAEIVCQ 269
>TC11310 similar to UP|BAD11331 (BAD11331) BRI1-KD interacting protein 102
(Fragment), partial (13%)
Length = 667
Score = 29.3 bits (64), Expect = 0.47
Identities = 17/63 (26%), Positives = 35/63 (54%), Gaps = 4/63 (6%)
Frame = +1
Query: 44 GQQHARKTKKKQVKDELDRL----KQAEKKKRRLEKALATSAAIISELEKKKQKKKEEQQ 99
G++ +K KKK+ ++ + ++AEK+K++ K + + +KK+KKK++Q
Sbjct: 76 GEKKEKKKKKKKDQENGEVASSDEEKAEKEKKKKHKVKVEDGSPDLDQSEKKKKKKKDQD 255
Query: 100 RLD 102
D
Sbjct: 256 AED 264
Score = 25.8 bits (55), Expect = 5.2
Identities = 13/43 (30%), Positives = 27/43 (62%)
Frame = +1
Query: 55 QVKDELDRLKQAEKKKRRLEKALATSAAIISELEKKKQKKKEE 97
+VK E++ K+ +KKK++ ++ A+ E +K++KKK +
Sbjct: 55 KVKVEVEGEKKEKKKKKKKDQENGEVASSDEEKAEKEKKKKHK 183
>TC16303 weakly similar to UP|AGA1_YEAST (P32323) A-agglutinin attachment
subunit precursor, partial (3%)
Length = 1235
Score = 27.7 bits (60), Expect = 1.4
Identities = 14/58 (24%), Positives = 32/58 (55%)
Frame = +1
Query: 48 ARKTKKKQVKDELDRLKQAEKKKRRLEKALATSAAIISELEKKKQKKKEEQQRLDEEG 105
A + ++ + DE++ L + KKK++ E++ A A ++ + + + EE L+ +G
Sbjct: 730 APQAEEGEEDDEINDLFKMGKKKKKNERSPAEIALLVENVMAELEVTAEEDAELNRQG 903
>TC9175 weakly similar to UP|INAD_DROME (Q24008)
Inactivation-no-after-potential D protein, partial (5%)
Length = 692
Score = 27.7 bits (60), Expect = 1.4
Identities = 20/61 (32%), Positives = 31/61 (50%), Gaps = 10/61 (16%)
Frame = +3
Query: 45 QQHARKTKKKQ--------VKDELDRLKQAEK--KKRRLEKALATSAAIISELEKKKQKK 94
Q+ +K KKK+ +K R KQ K KKR ++K + T + + KKK++K
Sbjct: 72 QRRKKKVKKKRNLQQRRVNLKRRKKRRKQQRKPRKKRVVKKRMITKKKRVVKKGKKKERK 251
Query: 95 K 95
K
Sbjct: 252 K 254
>TC14491 UP|Q43781 (Q43781) Aspartate aminotransferase , complete
Length = 1823
Score = 27.3 bits (59), Expect = 1.8
Identities = 14/51 (27%), Positives = 26/51 (50%)
Frame = -2
Query: 45 QQHARKTKKKQVKDELDRLKQAEKKKRRLEKALATSAAIISELEKKKQKKK 95
++H R +++ K L++ + +E R E+ + L KKK+KKK
Sbjct: 238 REHERDERRRNQKRRLEKERCSENPFDRCERGGLRER*VCDRLGKKKKKKK 86
>AV410716
Length = 424
Score = 27.3 bits (59), Expect = 1.8
Identities = 14/44 (31%), Positives = 26/44 (58%)
Frame = +3
Query: 67 EKKKRRLEKALATSAAIISELEKKKQKKKEEQQRLDEEGAAIAE 110
EKKK++L+K +KKK+KKK++Q+++ E + +
Sbjct: 153 EKKKKKLKK------------KKKKKKKKKKQRKMVAEAEIVCQ 248
>BP034655
Length = 517
Score = 26.9 bits (58), Expect = 2.3
Identities = 14/47 (29%), Positives = 28/47 (58%)
Frame = +2
Query: 55 QVKDELDRLKQAEKKKRRLEKALATSAAIISELEKKKQKKKEEQQRL 101
Q+ D +K+ +KKK++ +K + +KKK+KKK++ +R+
Sbjct: 119 QILDSPTTMKRKKKKKKKKKK---------KKKKKKKKKKKKKTKRM 232
Score = 25.0 bits (53), Expect = 8.8
Identities = 10/29 (34%), Positives = 20/29 (68%)
Frame = +2
Query: 49 RKTKKKQVKDELDRLKQAEKKKRRLEKAL 77
RK KKK+ K + + K+ +KKK++ ++ +
Sbjct: 149 RKKKKKKKKKKKKKKKKKKKKKKKTKRMM 235
>TC18924 homologue to UP|Q94FN3 (Q94FN3) Phosphatidylinositol transfer-like
protein II, complete
Length = 1976
Score = 26.6 bits (57), Expect = 3.0
Identities = 12/26 (46%), Positives = 18/26 (69%)
Frame = +3
Query: 70 KRRLEKALATSAAIISELEKKKQKKK 95
K+ LE +LA + + +EKKK+KKK
Sbjct: 1599 KKALEDSLAKQEELSAYIEKKKKKKK 1676
>BP072343
Length = 517
Score = 26.6 bits (57), Expect = 3.0
Identities = 17/46 (36%), Positives = 26/46 (55%), Gaps = 1/46 (2%)
Frame = +2
Query: 67 EKKKRRLEKALATSAAIIS-ELEKKKQKKKEEQQRLDEEGAAIAEA 111
E+KK+ EKA A +A E EKK +K K++ ++E + EA
Sbjct: 107 ERKKK*AEKAAAKAALKAQKEAEKKLKKAKKKAGIVEEPADEVVEA 244
>TC17589
Length = 541
Score = 26.2 bits (56), Expect = 3.9
Identities = 19/64 (29%), Positives = 33/64 (50%)
Frame = +3
Query: 44 GQQHARKTKKKQVKDELDRLKQAEKKKRRLEKALATSAAIISELEKKKQKKKEEQQRLDE 103
GQ A T+++ + L Q E+K+ LE +L + KKK+KKK+++ +
Sbjct: 132 GQVEALNTQQRTT-EILSDPSQDEEKRVMLEHSLIHDNRKLGISIKKKKKKKKKKDGFGK 308
Query: 104 EGAA 107
+ AA
Sbjct: 309 KSAA 320
>TC12685 weakly similar to UP|Q7QA92 (Q7QA92) AgCP13961 (Fragment), partial
(9%)
Length = 547
Score = 26.2 bits (56), Expect = 3.9
Identities = 13/39 (33%), Positives = 23/39 (58%)
Frame = +2
Query: 118 LDEDSDDSYKVECKTWDDYNNNLDFFMSGKRACFPNLDG 156
+DE+ D+ ++ + K +DDYN++ D +G F DG
Sbjct: 167 VDEEDDEEFE-DAKEFDDYNDDDDAPENGYAGNFEEDDG 280
>TC8740
Length = 864
Score = 26.2 bits (56), Expect = 3.9
Identities = 13/39 (33%), Positives = 20/39 (50%)
Frame = +2
Query: 66 AEKKKRRLEKALATSAAIISELEKKKQKKKEEQQRLDEE 104
AEK R+E+ + SEL++ K + E QR+ E
Sbjct: 458 AEKASGRVERGDRRKRILASELQRAKDRTLSEDQRIAAE 574
>BE122625
Length = 384
Score = 26.2 bits (56), Expect = 3.9
Identities = 9/20 (45%), Positives = 12/20 (60%)
Frame = +1
Query: 133 WDDYNNNLDFFMSGKRACFP 152
W DY++N + G RAC P
Sbjct: 256 WTDYSSNCNLNCHGHRACLP 315
>TC9825 similar to UP|Q9ZRD8 (Q9ZRD8) GMFP5 (Fragment), partial (44%)
Length = 848
Score = 25.8 bits (55), Expect = 5.2
Identities = 11/17 (64%), Positives = 12/17 (69%)
Frame = +3
Query: 167 KWSISFRTVRKQCTRTT 183
KWSIS R R+QC TT
Sbjct: 273 KWSISTRYKRRQCIGTT 323
>CB828378
Length = 554
Score = 25.8 bits (55), Expect = 5.2
Identities = 12/23 (52%), Positives = 16/23 (69%)
Frame = +1
Query: 73 LEKALATSAAIISELEKKKQKKK 95
LE +LA + + +EKKKQKKK
Sbjct: 247 LEDSLAKQEVLSAYVEKKKQKKK 315
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.328 0.137 0.418
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,067,051
Number of Sequences: 28460
Number of extensions: 38227
Number of successful extensions: 429
Number of sequences better than 10.0: 49
Number of HSP's better than 10.0 without gapping: 346
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 386
length of query: 194
length of database: 4,897,600
effective HSP length: 85
effective length of query: 109
effective length of database: 2,478,500
effective search space: 270156500
effective search space used: 270156500
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 53 (25.0 bits)
Medicago: description of AC121237.5