BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC121233.6 - phase: 2
(123 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BI418429 107 1e-26
BF177884 44 8e-06
BP057287 41 5e-05
TC16121 similar to UP|Q9MBD6 (Q9MBD6) Ntdin, partial (26%) 38 4e-04
TC20103 weakly similar to UP|Q8LCM5 (Q8LCM5) Peptidyl-prolyl cis... 34 0.006
TC9791 31 0.069
BP037828 26 2.2
TC14185 similar to UP|Q7Y256 (Q7Y256) Beta-cyanoalanine synthase... 25 3.8
TC15973 homologue to GB|AAL15278.1|16323087|AY057647 AT3g01490/F... 25 3.8
TC19889 weakly similar to UP|Q8W338 (Q8W338) TGF beta receptor a... 25 3.8
TC10326 similar to AAQ89612 (AAQ89612) At5g26850, partial (21%) 25 3.8
TC14018 similar to UP|Q43465 (Q43465) Protein kinase 2, partial ... 25 5.0
CN825351 24 6.5
TC16708 24 8.5
TC16524 similar to GB|AAL69537.1|18491137|AY074839 AT5g45420/MFC... 24 8.5
>BI418429
Length = 474
Score = 107 bits (267), Expect(2) = 1e-26
Identities = 56/102 (54%), Positives = 71/102 (68%), Gaps = 1/102 (0%)
Frame = +3
Query: 4 EVVTVDVLATKSLIKTT-HVYLDVRTVEEFQKGHVDSEKIINIAYMFNTPEGRVKNPEFL 62
+VV VDV A K L+ ++ + YLDVRTVEEF K HVD+ +NIAYMF+T GRVKNP+FL
Sbjct: 72 DVVNVDVEAAKDLLNSSGYRYLDVRTVEEFNKSHVDNA--LNIAYMFSTEAGRVKNPDFL 245
Query: 63 KEVSSLCNKEDHLIVGCQSGVRSVYATADLLAEGFKDVYNMG 104
+V+++C EDHL+V C SG R A DLL G+K V G
Sbjct: 246 DQVAAVCKSEDHLLVACNSGGRGRKAVIDLLDSGYKHVITWG 371
Score = 26.6 bits (57), Expect(2) = 1e-26
Identities = 10/15 (66%), Positives = 10/15 (66%)
Frame = +2
Query: 101 YNMGGGYLEWVKKEF 115
YNMGGGY WV F
Sbjct: 359 YNMGGGYSAWVDAGF 403
>BF177884
Length = 509
Score = 43.9 bits (102), Expect = 8e-06
Identities = 37/121 (30%), Positives = 52/121 (42%), Gaps = 22/121 (18%)
Frame = +2
Query: 2 KTEVVTVDVLATKSLIKTT-HVYLDVRTVEEFQKGHV---------------DSEKII-- 43
K EV V+ K LI + LDVR + +F++ H+ D II
Sbjct: 140 KAEVKFVNAEQAKELIAVDGYSVLDVRDITQFERAHIKSCYHVPLFVENKDNDPGTIIKR 319
Query: 44 ----NIAYMFNTPEGRVKNPEFLKEVSSLCNKEDHLIVGCQSGVRSVYATADLLAEGFKD 99
N + +F NPEF++ V S E ++V CQ G+RS A L GF+D
Sbjct: 320 TLHNNFSGLFYGLPFTKPNPEFVQSVKSQIPPESKVLVVCQEGLRSTAAADRLEKAGFED 499
Query: 100 V 100
V
Sbjct: 500 V 502
>BP057287
Length = 531
Score = 41.2 bits (95), Expect = 5e-05
Identities = 16/25 (64%), Positives = 21/25 (84%)
Frame = -3
Query: 91 DLLAEGFKDVYNMGGGYLEWVKKEF 115
D+ +GFKDV NMGGGY++W+K EF
Sbjct: 250 DVNWQGFKDVSNMGGGYVDWLKNEF 176
>TC16121 similar to UP|Q9MBD6 (Q9MBD6) Ntdin, partial (26%)
Length = 504
Score = 38.1 bits (87), Expect = 4e-04
Identities = 21/43 (48%), Positives = 23/43 (52%)
Frame = +1
Query: 7 TVDVLATKSLIKTTHVYLDVRTVEEFQKGHVDSEKIINIAYMF 49
+V V L+ H YLDVRT EEF GH INI YMF
Sbjct: 370 SVPVRVAHELLLAGHKYLDVRTPEEFDAGHAPG--AINIPYMF 492
>TC20103 weakly similar to UP|Q8LCM5 (Q8LCM5) Peptidyl-prolyl cis-trans
isomerase-like protein, partial (25%)
Length = 563
Score = 34.3 bits (77), Expect = 0.006
Identities = 16/43 (37%), Positives = 26/43 (60%)
Frame = +2
Query: 64 EVSSLCNKEDHLIVGCQSGVRSVYATADLLAEGFKDVYNMGGG 106
EV++ + + V C GVRS+ + L ++GF+ VYN+ GG
Sbjct: 53 EVTTKFDPQKDTYVMCHHGVRSLQVSKWLQSQGFRKVYNVSGG 181
>TC9791
Length = 593
Score = 30.8 bits (68), Expect = 0.069
Identities = 15/32 (46%), Positives = 21/32 (64%)
Frame = +2
Query: 75 LIVGCQSGVRSVYATADLLAEGFKDVYNMGGG 106
LIV CQ GVRS+ A L G+K+++ + GG
Sbjct: 2 LIVACQKGVRSLAACELLYNAGYKNLFWVQGG 97
>BP037828
Length = 554
Score = 25.8 bits (55), Expect = 2.2
Identities = 10/20 (50%), Positives = 13/20 (65%)
Frame = -1
Query: 52 PEGRVKNPEFLKEVSSLCNK 71
P GR+ PEFL ++ L NK
Sbjct: 452 PRGRIYTPEFLTKLQPLLNK 393
>TC14185 similar to UP|Q7Y256 (Q7Y256) Beta-cyanoalanine synthase, partial
(96%)
Length = 1506
Score = 25.0 bits (53), Expect = 3.8
Identities = 18/62 (29%), Positives = 29/62 (46%), Gaps = 2/62 (3%)
Frame = +1
Query: 16 LIKTTHVYLDVRTVEE--FQKGHVDSEKIINIAYMFNTPEGRVKNPEFLKEVSSLCNKED 73
LI T H R + FQ+ ++EK+ ++ P G +K F+ E+ S C+K
Sbjct: 1093 LIVTIHPSFGERYLSSVLFQELRDEAEKMQPVSVD*CPPSGLIKLESFVVELQSCCSKSC 1272
Query: 74 HL 75
L
Sbjct: 1273 QL 1278
>TC15973 homologue to GB|AAL15278.1|16323087|AY057647 AT3g01490/F4P13_4
{Arabidopsis thaliana;}, partial (49%)
Length = 944
Score = 25.0 bits (53), Expect = 3.8
Identities = 13/41 (31%), Positives = 20/41 (48%)
Frame = +2
Query: 41 KIINIAYMFNTPEGRVKNPEFLKEVSSLCNKEDHLIVGCQS 81
K + + +PE +N E L E CN+E ++GC S
Sbjct: 368 KFLRNYFSRGSPEFETRNTEMLPEFPCECNEE---MLGCDS 481
>TC19889 weakly similar to UP|Q8W338 (Q8W338) TGF beta receptor associated
protein-like protein, partial (11%)
Length = 577
Score = 25.0 bits (53), Expect = 3.8
Identities = 16/65 (24%), Positives = 27/65 (40%)
Frame = +1
Query: 59 PEFLKEVSSLCNKEDHLIVGCQSGVRSVYATADLLAEGFKDVYNMGGGYLEWVKKEFPVK 118
P F ++ S+ + L+VGC G +Y+ + K Y + + KK V
Sbjct: 85 PNFPGKIESVVTYDSKLLVGCSDGSLRIYSPEPDSPDRVKPPYVLEKNLTGFAKKPI-VS 261
Query: 119 IHVTE 123
+ V E
Sbjct: 262 LEVLE 276
>TC10326 similar to AAQ89612 (AAQ89612) At5g26850, partial (21%)
Length = 746
Score = 25.0 bits (53), Expect = 3.8
Identities = 23/100 (23%), Positives = 46/100 (46%), Gaps = 9/100 (9%)
Frame = +2
Query: 1 SKTEVVTVDVLATKSLIKTTHV--YLDVRTVEEFQKGHVDSEKIINIAYMFNTPEGRVKN 58
S+ ++V + A K+ + + YL+ R +E + GH+ II ++ +V+
Sbjct: 278 SERKIVKLCEYAAKNPFRIPKIAKYLEERCYKELRSGHIKLVTIITESFNKLLSICKVQI 457
Query: 59 PEFLKEVSSLCNK-----EDHLI--VGCQSGVRSVYATAD 91
F +V ++ + +D I +GCQS R ++ D
Sbjct: 458 AYFALDVLNVITELLGYSKDETIQTLGCQSLTRFIFCQVD 577
>TC14018 similar to UP|Q43465 (Q43465) Protein kinase 2, partial (27%)
Length = 559
Score = 24.6 bits (52), Expect = 5.0
Identities = 15/46 (32%), Positives = 26/46 (55%)
Frame = -3
Query: 49 FNTPEGRVKNPEFLKEVSSLCNKEDHLIVGCQSGVRSVYATADLLA 94
+ P GR+ N + K++S+LC + IVG GV + Y + + L+
Sbjct: 128 YGDPLGRISNKDSRKKISTLC--RNAYIVG--YGVLNTYYSPNSLS 3
>CN825351
Length = 638
Score = 24.3 bits (51), Expect = 6.5
Identities = 12/44 (27%), Positives = 23/44 (52%)
Frame = +1
Query: 72 EDHLIVGCQSGVRSVYATADLLAEGFKDVYNMGGGYLEWVKKEF 115
E+ L+ SG+ ++ A+ DL DVY+ L W ++++
Sbjct: 289 EEELMALPLSGIEAILASDDLQVASDDDVYDFA---LNWARQKY 411
>TC16708
Length = 515
Score = 23.9 bits (50), Expect = 8.5
Identities = 10/33 (30%), Positives = 18/33 (54%)
Frame = -2
Query: 36 HVDSEKIINIAYMFNTPEGRVKNPEFLKEVSSL 68
HV ++ I + G +KNP+FL ++S +
Sbjct: 478 HVPAQIIQICESKISPSMGNIKNPKFLNQISKM 380
>TC16524 similar to GB|AAL69537.1|18491137|AY074839 AT5g45420/MFC19_9
{Arabidopsis thaliana;}, partial (41%)
Length = 997
Score = 23.9 bits (50), Expect = 8.5
Identities = 9/17 (52%), Positives = 13/17 (75%)
Frame = -3
Query: 36 HVDSEKIINIAYMFNTP 52
HV+ +KII+I+Y F P
Sbjct: 929 HVNKQKIISISYKFINP 879
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.316 0.135 0.386
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,919,390
Number of Sequences: 28460
Number of extensions: 21865
Number of successful extensions: 107
Number of sequences better than 10.0: 30
Number of HSP's better than 10.0 without gapping: 105
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 106
length of query: 123
length of database: 4,897,600
effective HSP length: 79
effective length of query: 44
effective length of database: 2,649,260
effective search space: 116567440
effective search space used: 116567440
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 49 (23.5 bits)
Medicago: description of AC121233.6