BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC121233.13 - phase: 0
(202 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC8428 similar to UP|Q9SNY0 (Q9SNY0) SCUTL2, partial (87%) 220 2e-58
TC20023 similar to GB|AAP13435.1|30023804|BT006327 At1g20030 {Ar... 216 3e-57
TC9212 similar to UP|TLPH_ARATH (P50699) Thaumatin-like protein ... 171 1e-43
TC14162 similar to UP|OLP1_LYCES (Q41350) Osmotin-like protein p... 164 1e-41
TC19049 similar to PIR|T05694|T05694 pathogenesis-related protei... 141 7e-35
TC7964 homologue to UP|O81926 (O81926) Thaumatin-like protein PR... 114 1e-26
TC16995 similar to PIR|T05493|T05493 pathogenesis-related protei... 102 5e-23
BP046128 96 6e-21
TC18115 weakly similar to UP|Q93XD4 (Q93XD4) Thaumatin-like prot... 95 7e-21
TC7965 homologue to UP|O81926 (O81926) Thaumatin-like protein PR... 74 1e-14
AV409685 68 1e-12
BP056986 62 9e-11
BP036640 60 2e-10
AV769280 59 6e-10
AW428867 33 0.034
AV410524 29 0.64
TC9222 similar to UP|Q9SS90 (Q9SS90) F4P13.19 protein, partial (... 29 0.64
TC7928 similar to UP|Q93YR3 (Q93YR3) HSP associated protein like... 28 0.84
TC18368 similar to GB|AAP13435.1|30023804|BT006327 At1g20030 {Ar... 27 1.9
BG662219 27 2.4
>TC8428 similar to UP|Q9SNY0 (Q9SNY0) SCUTL2, partial (87%)
Length = 1131
Score = 220 bits (560), Expect = 2e-58
Identities = 106/189 (56%), Positives = 129/189 (68%), Gaps = 2/189 (1%)
Frame = +1
Query: 2 PTSWSGRIWGRTLCSTDPSGKFSCITGDCGSSKVECTGSGAIPPATLAEFTLNGANG--L 59
P+ WSGR W RT C+ D +G +C TGDC ++CTG G +PP TLAEFT+ A+
Sbjct: 259 PSGWSGRFWARTGCNFDGTGNGNCATGDCAGG-LKCTGGG-VPPVTLAEFTIGTASNGNK 432
Query: 60 DFYDVSLVDGYNLPITVEPSGGSGNCTTTGCLVDLNGACPTELKVKIAAEEEGSDEKSVA 119
DFYDVSLVDGYN+ + V +GG+G+C GC+ DLNG+CP EL+VK E S + VA
Sbjct: 433 DFYDVSLVDGYNVGMGVRATGGTGDCQYAGCVADLNGSCPAELQVK----GENSTGEVVA 600
Query: 120 CKSACEAFGDPLYCCNGAYGTPQTCKPSSYSQFFKSACPRAYSYAYDDGTSTFTCSSADY 179
CKSAC AF +CC G + TP TC P+ YS+ FKSACP AYSYAYDD TST TCS +DY
Sbjct: 601 CKSACAAFNTTEFCCTGEHATPATCSPTHYSEIFKSACPTAYSYAYDDATSTCTCSGSDY 780
Query: 180 LITFCSTHS 188
ITFC S
Sbjct: 781 SITFCPNGS 807
>TC20023 similar to GB|AAP13435.1|30023804|BT006327 At1g20030 {Arabidopsis
thaliana;}, partial (46%)
Length = 418
Score = 216 bits (549), Expect = 3e-57
Identities = 101/142 (71%), Positives = 118/142 (82%), Gaps = 1/142 (0%)
Frame = +3
Query: 31 GSSKVECTGSGAIPPATLAEFTLNGANGLDFYDVSLVDGYNLPITVEPSGGSGN-CTTTG 89
GS K+EC GSGA PPATLAEFTL+G++GLDF+DVSLVDGYN+P+ V P GGSGN CT TG
Sbjct: 6 GSGKLECAGSGAAPPATLAEFTLDGSDGLDFFDVSLVDGYNVPMLVVPEGGSGNNCTATG 185
Query: 90 CLVDLNGACPTELKVKIAAEEEGSDEKSVACKSACEAFGDPLYCCNGAYGTPQTCKPSSY 149
C+ DLNGACP+EL+V +++VACKSACEAFG P YCC+GAYG+P TCKPS+Y
Sbjct: 186 CVGDLNGACPSELRVTSV-----DGKRNVACKSACEAFGSPQYCCSGAYGSPNTCKPSTY 350
Query: 150 SQFFKSACPRAYSYAYDDGTST 171
SQ FKSACPRAYSYAYDD +ST
Sbjct: 351 SQVFKSACPRAYSYAYDDKSST 416
>TC9212 similar to UP|TLPH_ARATH (P50699) Thaumatin-like protein precursor,
partial (51%)
Length = 662
Score = 171 bits (432), Expect = 1e-43
Identities = 77/128 (60%), Positives = 95/128 (74%)
Frame = +1
Query: 60 DFYDVSLVDGYNLPITVEPSGGSGNCTTTGCLVDLNGACPTELKVKIAAEEEGSDEKSVA 119
DFYDVSLVDGYNLPI++ P GSG C+ GC+ DLN CP L+V+ +++ VA
Sbjct: 1 DFYDVSLVDGYNLPISITPLKGSGKCSYAGCVRDLNIMCPVGLQVR-----SRDNKRVVA 165
Query: 120 CKSACEAFGDPLYCCNGAYGTPQTCKPSSYSQFFKSACPRAYSYAYDDGTSTFTCSSADY 179
CKSAC AF P YCC G+YG+PQ+CKP++YS+ FK+ACP+AYSYAYDD TS TC+ A Y
Sbjct: 166 CKSACAAFNSPRYCCTGSYGSPQSCKPTAYSRIFKTACPKAYSYAYDDPTSISTCTHASY 345
Query: 180 LITFCSTH 187
LITFC H
Sbjct: 346 LITFCPHH 369
>TC14162 similar to UP|OLP1_LYCES (Q41350) Osmotin-like protein precursor,
partial (87%)
Length = 1232
Score = 164 bits (414), Expect = 1e-41
Identities = 84/185 (45%), Positives = 108/185 (57%), Gaps = 3/185 (1%)
Frame = +1
Query: 3 TSWSGRIWGRTLCSTDPSGKFSCITGDCGSSKVECTGSGAIPPATLAEFTLNGANGLDF- 61
T WSGR+W RT C+ +F+C +GDCG +++C G+G PATLA+F ++ N DF
Sbjct: 244 THWSGRVWARTGCTYAAGNRFTCASGDCG-GRLQCAGAGGSAPATLAQFDMHHGNA-DFS 417
Query: 62 -YDVSLVDGYNLPITVEPSGGSGNCTTTGCLVDLNGACPTELKVKIAAEEEGSDEKSVAC 120
Y VSLVDG+N+P+TV P G G C GC DL CP L ++ A VAC
Sbjct: 418 SYSVSLVDGFNVPMTVTPHEGKGVCPVVGCRSDLLATCPPVLHHRVPAVH----GPVVAC 585
Query: 121 KSACEAFGDPLYCCNGAYGTPQTCKPSSYSQFFKSACPRAYSYAYDDGTSTFTCSSADYL 180
KS CEAF CC + QTCKPS YS FFK ACP +++A+D + T CSS L
Sbjct: 586 KSGCEAFHSDELCCRNHFNNAQTCKPSVYSTFFKHACPATFTFAHDTPSLTHECSSPREL 765
Query: 181 -ITFC 184
+ FC
Sbjct: 766 KVIFC 780
>TC19049 similar to PIR|T05694|T05694 pathogenesis-related protein
F20M13.220 - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (36%)
Length = 861
Score = 141 bits (356), Expect = 7e-35
Identities = 64/101 (63%), Positives = 75/101 (73%)
Frame = +3
Query: 2 PTSWSGRIWGRTLCSTDPSGKFSCITGDCGSSKVECTGSGAIPPATLAEFTLNGANGLDF 61
PT WSGR W RT C D SG+ +C T DCGS ++ C G+ A+PPATLAEFTL G +DF
Sbjct: 561 PTGWSGRFWARTGCQFDSSGRGTCATADCGSGEINCNGATALPPATLAEFTL-GTGSMDF 737
Query: 62 YDVSLVDGYNLPITVEPSGGSGNCTTTGCLVDLNGACPTEL 102
YDVSLVDGYNLP+ V SGGSG+C+ TGC DLN CP+EL
Sbjct: 738 YDVSLVDGYNLPMMVVASGGSGSCSATGCGADLNRKCPSEL 860
>TC7964 homologue to UP|O81926 (O81926) Thaumatin-like protein PR-5b
precursor (Fragment), partial (74%)
Length = 778
Score = 114 bits (285), Expect = 1e-26
Identities = 61/150 (40%), Positives = 80/150 (52%)
Frame = +2
Query: 8 RIWGRTLCSTDPSGKFSCITGDCGSSKVECTGSGAIPPATLAEFTLNGANGLDFYDVSLV 67
RIWGRT C+ D +G+ C TGDC + + C G G +PP TLAEF LN DFYD+SLV
Sbjct: 230 RIWGRTGCNFDGAGRGRCQTGDC-TGGLNCQGWG-VPPNTLAEFALNQYGNQDFYDISLV 403
Query: 68 DGYNLPITVEPSGGSGNCTTTGCLVDLNGACPTELKVKIAAEEEGSDEKSVACKSACEAF 127
DG+N+P+ P +G C C D+NG CP EL+ C + C A+
Sbjct: 404 DGFNIPMDFFPL--NGGCHKISCTADINGQCPNELRA------------PGGCNNPCTAY 541
Query: 128 GDPLYCCNGAYGTPQTCKPSSYSQFFKSAC 157
+CC G TC P+ + +FFK C
Sbjct: 542 KTNEFCCTNGQG---TCGPN*FLRFFKDRC 622
>TC16995 similar to PIR|T05493|T05493 pathogenesis-related protein 19K4.140
- Arabidopsis thaliana {Arabidopsis thaliana;}, partial
(22%)
Length = 614
Score = 102 bits (254), Expect = 5e-23
Identities = 43/59 (72%), Positives = 47/59 (78%)
Frame = +1
Query: 130 PLYCCNGAYGTPQTCKPSSYSQFFKSACPRAYSYAYDDGTSTFTCSSADYLITFCSTHS 188
P YCCNGA+ P TCKPS YS+ FKSACP +YSYAYDD TSTFTCS ADY ITFC + S
Sbjct: 1 PEYCCNGAFSNPSTCKPSVYSEMFKSACPESYSYAYDDATSTFTCSGADYTITFCPSSS 177
>BP046128
Length = 514
Score = 95.5 bits (236), Expect = 6e-21
Identities = 40/67 (59%), Positives = 47/67 (69%)
Frame = -1
Query: 118 VACKSACEAFGDPLYCCNGAYGTPQTCKPSSYSQFFKSACPRAYSYAYDDGTSTFTCSSA 177
V C SAC AF + YCC G + +P+ CKPS Y+ FFK CP AYSY DD +STF+CS A
Sbjct: 511 VGCNSACVAFNEAKYCCTGEFESPELCKPSEYALFFKKKCPLAYSYPSDDESSTFSCSRA 332
Query: 178 DYLITFC 184
DY ITFC
Sbjct: 331 DYTITFC 311
>TC18115 weakly similar to UP|Q93XD4 (Q93XD4) Thaumatin-like protein,
partial (63%)
Length = 792
Score = 95.1 bits (235), Expect = 7e-21
Identities = 46/81 (56%), Positives = 59/81 (72%)
Frame = +2
Query: 6 SGRIWGRTLCSTDPSGKFSCITGDCGSSKVECTGSGAIPPATLAEFTLNGANGLDFYDVS 65
SGRIWGRT C+ D SG+ SC TGDCG + + C+ SG PP TLAEFTLNG N D+YD+S
Sbjct: 185 SGRIWGRTGCNFDGSGRGSCQTGDCGGA-LSCSLSGR-PPTTLAEFTLNGGNNQDYYDIS 358
Query: 66 LVDGYNLPITVEPSGGSGNCT 86
++DG+N+P+ P+ S CT
Sbjct: 359 VIDGFNVPMEFSPT--SNGCT 415
Score = 28.1 bits (61), Expect = 1.1
Identities = 28/109 (25%), Positives = 40/109 (36%), Gaps = 5/109 (4%)
Frame = +2
Query: 81 GSGNCTTTGC----LVDLNGACPTELKVKIAAEEEGSDEKSVACKSACEAFGDPLYCCNG 136
G G+C T C L+G PT L D + S + F P+
Sbjct: 230 GRGSCQTGDCGGALSCSLSGRPPTTLAEFTLNGGNNQDYYDI---SVIDGFNVPMEFSPT 400
Query: 137 AYGTPQTCKPSSYSQFFKSACPRAYSYAYDDGTSTFTC-SSADYLITFC 184
+ G ++ + + CP AY Y DD T T +C Y + FC
Sbjct: 401 SNGCTRSLRCHA------DKCPDAYLYPSDD-TKTHSCPGGTHYRVVFC 526
>TC7965 homologue to UP|O81926 (O81926) Thaumatin-like protein PR-5b
precursor (Fragment), partial (37%)
Length = 633
Score = 74.3 bits (181), Expect = 1e-14
Identities = 37/111 (33%), Positives = 55/111 (49%), Gaps = 1/111 (0%)
Frame = +2
Query: 90 CLVDLNGACPTELKVKIAAEEEGSDEKSVACKSACEAFGDPLYCCNGAYGTPQTCKPSSY 149
C D+NG CP EL+ C + C + +CC GT C P+++
Sbjct: 8 CTADINGQCPNELRAP------------GGCNNPCTVYKTNEFCCTNGQGT---CGPTNF 142
Query: 150 SQFFKSACPRAYSYAYDDGTSTFTC-SSADYLITFCSTHSKGYCIVVQSSD 199
S+FFK C +YSY DD TSTFTC + ++Y + FC S +++ +D
Sbjct: 143 SRFFKDRCHDSYSYPQDDPTSTFTCPAGSNYKVVFCPLGSPHVTLMMPGND 295
>AV409685
Length = 270
Score = 68.2 bits (165), Expect = 1e-12
Identities = 25/36 (69%), Positives = 30/36 (82%)
Frame = +2
Query: 2 PTSWSGRIWGRTLCSTDPSGKFSCITGDCGSSKVEC 37
P +W GR WGRTLCS D +GKF+C+TGDCGS K+EC
Sbjct: 161 PANWGGRFWGRTLCSQDSAGKFTCVTGDCGSGKLEC 268
>BP056986
Length = 450
Score = 61.6 bits (148), Expect = 9e-11
Identities = 25/40 (62%), Positives = 29/40 (72%)
Frame = -3
Query: 153 FKSACPRAYSYAYDDGTSTFTCSSADYLITFCSTHSKGYC 192
FK ACP +YSYAYDD TS FTCS DY+I FC++ K C
Sbjct: 445 FKEACPTSYSYAYDDPTSIFTCSGTDYVIAFCASRKKQVC 326
>BP036640
Length = 468
Score = 60.5 bits (145), Expect = 2e-10
Identities = 28/77 (36%), Positives = 43/77 (55%), Gaps = 1/77 (1%)
Frame = -2
Query: 124 CEAFGDPLYCCNGAYGTPQTCKPSSYSQFFKSACPRAYSYAYDDGTSTFTC-SSADYLIT 182
C + +CC GT C P+++S+FFK C +YSY DD TSTFTC + ++Y +
Sbjct: 464 CTVYKTNEFCCTNGQGT---CGPTNFSRFFKDRCHDSYSYPQDDPTSTFTCPAGSNYKVV 294
Query: 183 FCSTHSKGYCIVVQSSD 199
FC S +++ +D
Sbjct: 293 FCPLGSPHVTLMMPGND 243
>AV769280
Length = 327
Score = 58.9 bits (141), Expect = 6e-10
Identities = 24/32 (75%), Positives = 27/32 (84%)
Frame = -3
Query: 157 CPRAYSYAYDDGTSTFTCSSADYLITFCSTHS 188
CP++YSYAYDD TSTFTCS ADY ITFC + S
Sbjct: 325 CPKSYSYAYDDATSTFTCSGADYTITFCPSSS 230
>AW428867
Length = 375
Score = 33.1 bits (74), Expect = 0.034
Identities = 12/22 (54%), Positives = 15/22 (67%)
Frame = +1
Query: 1 MPTSWSGRIWGRTLCSTDPSGK 22
+P WSGR WGR C+ D SG+
Sbjct: 253 LPALWSGRFWGRHDCTFDASGR 318
>AV410524
Length = 402
Score = 28.9 bits (63), Expect = 0.64
Identities = 10/17 (58%), Positives = 10/17 (58%)
Frame = +3
Query: 2 PTSWSGRIWGRTLCSTD 18
P WSGR W RT C D
Sbjct: 348 PAGWSGRFWARTGCKFD 398
>TC9222 similar to UP|Q9SS90 (Q9SS90) F4P13.19 protein, partial (12%)
Length = 564
Score = 28.9 bits (63), Expect = 0.64
Identities = 20/64 (31%), Positives = 32/64 (49%), Gaps = 4/64 (6%)
Frame = +1
Query: 130 PLYCCNGAYGTPQTCKPSSYSQFFKSACPRAYSYAYDDGT---STFTCSSAD-YLITFCS 185
PLY +Y +P+T + SS+ P A S+ +D GT ++F S++D + C
Sbjct: 31 PLYGATASYNSPKTSRQSSFR-------PSAPSHRHDIGTNPPASFASSASDNQVCPICL 189
Query: 186 THSK 189
T K
Sbjct: 190 TDPK 201
>TC7928 similar to UP|Q93YR3 (Q93YR3) HSP associated protein like, partial
(52%)
Length = 765
Score = 28.5 bits (62), Expect = 0.84
Identities = 26/85 (30%), Positives = 40/85 (46%), Gaps = 10/85 (11%)
Frame = -1
Query: 102 LKVKIAAEEEGSDEKSVACKSA-CEAF---------GDPLYCCNGAYGTPQTCKPSSYSQ 151
+K+ IA+ + S+ S+A A CEA G P++C + G+ T PSS
Sbjct: 423 VKLSIASSKLPSEIASIALALANCEASRLSSVTSTEGSPIFCGGSSSGS--TISPSS--- 259
Query: 152 FFKSACPRAYSYAYDDGTSTFTCSS 176
+ + + G+STFTCSS
Sbjct: 258 ----SISDSTISSSSSGSSTFTCSS 196
>TC18368 similar to GB|AAP13435.1|30023804|BT006327 At1g20030 {Arabidopsis
thaliana;}, partial (7%)
Length = 603
Score = 27.3 bits (59), Expect = 1.9
Identities = 10/12 (83%), Positives = 11/12 (91%)
Frame = +3
Query: 173 TCSSADYLITFC 184
TC+SADY ITFC
Sbjct: 3 TCASADYTITFC 38
>BG662219
Length = 366
Score = 26.9 bits (58), Expect = 2.4
Identities = 14/38 (36%), Positives = 17/38 (43%)
Frame = +2
Query: 4 SWSGRIWGRTLCSTDPSGKFSCITGDCGSSKVECTGSG 41
+WSG+ DP G F CI G + VE G G
Sbjct: 212 TWSGK---------DPEGLFPCILGHEAAGVVESVGEG 298
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.317 0.134 0.431
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,805,996
Number of Sequences: 28460
Number of extensions: 76525
Number of successful extensions: 387
Number of sequences better than 10.0: 59
Number of HSP's better than 10.0 without gapping: 370
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 373
length of query: 202
length of database: 4,897,600
effective HSP length: 86
effective length of query: 116
effective length of database: 2,450,040
effective search space: 284204640
effective search space used: 284204640
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)
Medicago: description of AC121233.13