BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC121233.12 - phase: 0
(107 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC8428 similar to UP|Q9SNY0 (Q9SNY0) SCUTL2, partial (87%) 135 1e-33
TC9212 similar to UP|TLPH_ARATH (P50699) Thaumatin-like protein ... 125 1e-30
TC20023 similar to GB|AAP13435.1|30023804|BT006327 At1g20030 {Ar... 124 2e-30
TC16995 similar to PIR|T05493|T05493 pathogenesis-related protei... 115 1e-27
TC7965 homologue to UP|O81926 (O81926) Thaumatin-like protein PR... 95 2e-21
BP046128 91 4e-20
TC14162 similar to UP|OLP1_LYCES (Q41350) Osmotin-like protein p... 79 2e-16
BP036640 71 3e-14
AV769280 67 6e-13
TC7964 homologue to UP|O81926 (O81926) Thaumatin-like protein PR... 60 7e-11
TC19049 similar to PIR|T05694|T05694 pathogenesis-related protei... 54 5e-09
BP056986 52 2e-08
TC18115 weakly similar to UP|Q93XD4 (Q93XD4) Thaumatin-like prot... 39 1e-04
BP057889 31 0.028
TC9780 similar to AAQ56830 (AAQ56830) At5g09670, partial (55%) 29 0.11
TC14715 29 0.14
TC12482 similar to UP|Q9TD47 (Q9TD47) NADH dehydrogenase subunit... 28 0.18
TC18368 similar to GB|AAP13435.1|30023804|BT006327 At1g20030 {Ar... 28 0.31
TC14830 similar to UP|Q8GVF6 (Q8GVF6) Pyrrolidone carboxyl pepti... 27 0.40
BP078538 27 0.40
>TC8428 similar to UP|Q9SNY0 (Q9SNY0) SCUTL2, partial (87%)
Length = 1131
Score = 135 bits (340), Expect = 1e-33
Identities = 62/110 (56%), Positives = 80/110 (72%), Gaps = 6/110 (5%)
Frame = +1
Query: 2 VETSGGSGSCQATGCGEDLNRRCPSELRVDGGD------ACNSACGAFGTPEYCCSGAFG 55
V +GG+G CQ GC DLN CP+EL+V G + AC SAC AF T E+CC+G
Sbjct: 481 VRATGGTGDCQYAGCVADLNGSCPAELQVKGENSTGEVVACKSACAAFNTTEFCCTGEHA 660
Query: 56 SPSSCSPSVYSEMFKSACPKSYSYAFDDATSTFTCTAADDYTITFCPSSS 105
+P++CSP+ YSE+FKSACP +YSYA+DDATST TC+ + DY+ITFCP+ S
Sbjct: 661 TPATCSPTHYSEIFKSACPTAYSYAYDDATSTCTCSGS-DYSITFCPNGS 807
>TC9212 similar to UP|TLPH_ARATH (P50699) Thaumatin-like protein precursor,
partial (51%)
Length = 662
Score = 125 bits (314), Expect = 1e-30
Identities = 58/101 (57%), Positives = 68/101 (66%), Gaps = 5/101 (4%)
Frame = +1
Query: 7 GSGSCQATGCGEDLNRRCPSELRVDGGD-----ACNSACGAFGTPEYCCSGAFGSPSSCS 61
GSG C GC DLN CP L+V D AC SAC AF +P YCC+G++GSP SC
Sbjct: 64 GSGKCSYAGCVRDLNIMCPVGLQVRSRDNKRVVACKSACAAFNSPRYCCTGSYGSPQSCK 243
Query: 62 PSVYSEMFKSACPKSYSYAFDDATSTFTCTAADDYTITFCP 102
P+ YS +FK+ACPK+YSYA+DD TS TCT A Y ITFCP
Sbjct: 244 PTAYSRIFKTACPKAYSYAYDDPTSISTCTHA-SYLITFCP 363
Score = 25.8 bits (55), Expect = 1.2
Identities = 12/32 (37%), Positives = 16/32 (49%)
Frame = -2
Query: 35 ACNSACGAFGTPEYCCSGAFGSPSSCSPSVYS 66
AC A G P+ CSG +P SC+ + S
Sbjct: 154 ACCHATSPAGPPDT*CSGPSRTPHSCTSQIPS 59
>TC20023 similar to GB|AAP13435.1|30023804|BT006327 At1g20030 {Arabidopsis
thaliana;}, partial (46%)
Length = 418
Score = 124 bits (311), Expect = 2e-30
Identities = 58/93 (62%), Positives = 71/93 (75%), Gaps = 6/93 (6%)
Frame = +3
Query: 1 MVETSGGSGS-CQATGCGEDLNRRCPSELRVDGGD-----ACNSACGAFGTPEYCCSGAF 54
+V GGSG+ C ATGC DLN CPSELRV D AC SAC AFG+P+YCCSGA+
Sbjct: 138 LVVPEGGSGNNCTATGCVGDLNGACPSELRVTSVDGKRNVACKSACEAFGSPQYCCSGAY 317
Query: 55 GSPSSCSPSVYSEMFKSACPKSYSYAFDDATST 87
GSP++C PS YS++FKSACP++YSYA+DD +ST
Sbjct: 318 GSPNTCKPSTYSQVFKSACPRAYSYAYDDKSST 416
>TC16995 similar to PIR|T05493|T05493 pathogenesis-related protein 19K4.140
- Arabidopsis thaliana {Arabidopsis thaliana;}, partial
(22%)
Length = 614
Score = 115 bits (288), Expect = 1e-27
Identities = 51/61 (83%), Positives = 57/61 (92%)
Frame = +1
Query: 46 PEYCCSGAFGSPSSCSPSVYSEMFKSACPKSYSYAFDDATSTFTCTAADDYTITFCPSSS 105
PEYCC+GAF +PS+C PSVYSEMFKSACP+SYSYA+DDATSTFTC+ A DYTITFCPSSS
Sbjct: 1 PEYCCNGAFSNPSTCKPSVYSEMFKSACPESYSYAYDDATSTFTCSGA-DYTITFCPSSS 177
Query: 106 P 106
P
Sbjct: 178 P 180
>TC7965 homologue to UP|O81926 (O81926) Thaumatin-like protein PR-5b
precursor (Fragment), partial (37%)
Length = 633
Score = 95.1 bits (235), Expect = 2e-21
Identities = 43/91 (47%), Positives = 55/91 (60%)
Frame = +2
Query: 16 CGEDLNRRCPSELRVDGGDACNSACGAFGTPEYCCSGAFGSPSSCSPSVYSEMFKSACPK 75
C D+N +CP+ELR GG CN+ C + T E+CC+ G+ C P+ +S FK C
Sbjct: 8 CTADINGQCPNELRAPGG--CNNPCTVYKTNEFCCTNGQGT---CGPTNFSRFFKDRCHD 172
Query: 76 SYSYAFDDATSTFTCTAADDYTITFCPSSSP 106
SYSY DD TSTFTC A +Y + FCP SP
Sbjct: 173 SYSYPQDDPTSTFTCPAGSNYKVVFCPLGSP 265
>BP046128
Length = 514
Score = 90.5 bits (223), Expect = 4e-20
Identities = 40/67 (59%), Positives = 47/67 (69%)
Frame = -1
Query: 36 CNSACGAFGTPEYCCSGAFGSPSSCSPSVYSEMFKSACPKSYSYAFDDATSTFTCTAADD 95
CNSAC AF +YCC+G F SP C PS Y+ FK CP +YSY DD +STF+C+ A D
Sbjct: 505 CNSACVAFNEAKYCCTGEFESPELCKPSEYALFFKKKCPLAYSYPSDDESSTFSCSRA-D 329
Query: 96 YTITFCP 102
YTITFCP
Sbjct: 328 YTITFCP 308
>TC14162 similar to UP|OLP1_LYCES (Q41350) Osmotin-like protein precursor,
partial (87%)
Length = 1232
Score = 78.6 bits (192), Expect = 2e-16
Identities = 39/101 (38%), Positives = 51/101 (49%), Gaps = 6/101 (5%)
Frame = +1
Query: 7 GSGSCQATGCGEDLNRRCPSELR-----VDGGD-ACNSACGAFGTPEYCCSGAFGSPSSC 60
G G C GC DL CP L V G AC S C AF + E CC F + +C
Sbjct: 478 GKGVCPVVGCRSDLLATCPPVLHHRVPAVHGPVVACKSGCEAFHSDELCCRNHFNNAQTC 657
Query: 61 SPSVYSEMFKSACPKSYSYAFDDATSTFTCTAADDYTITFC 101
PSVYS FK ACP ++++A D + T C++ + + FC
Sbjct: 658 KPSVYSTFFKHACPATFTFAHDTPSLTHECSSPRELKVIFC 780
>BP036640
Length = 468
Score = 70.9 bits (172), Expect = 3e-14
Identities = 31/67 (46%), Positives = 39/67 (57%)
Frame = -2
Query: 40 CGAFGTPEYCCSGAFGSPSSCSPSVYSEMFKSACPKSYSYAFDDATSTFTCTAADDYTIT 99
C + T E+CC+ G+ C P+ +S FK C SYSY DD TSTFTC A +Y +
Sbjct: 464 CTVYKTNEFCCTNGQGT---CGPTNFSRFFKDRCHDSYSYPQDDPTSTFTCPAGSNYKVV 294
Query: 100 FCPSSSP 106
FCP SP
Sbjct: 293 FCPLGSP 273
>AV769280
Length = 327
Score = 66.6 bits (161), Expect = 6e-13
Identities = 30/34 (88%), Positives = 32/34 (93%)
Frame = -3
Query: 73 CPKSYSYAFDDATSTFTCTAADDYTITFCPSSSP 106
CPKSYSYA+DDATSTFTC+ A DYTITFCPSSSP
Sbjct: 325 CPKSYSYAYDDATSTFTCSGA-DYTITFCPSSSP 227
>TC7964 homologue to UP|O81926 (O81926) Thaumatin-like protein PR-5b
precursor (Fragment), partial (74%)
Length = 778
Score = 59.7 bits (143), Expect = 7e-11
Identities = 27/69 (39%), Positives = 38/69 (54%)
Frame = +2
Query: 8 SGSCQATGCGEDLNRRCPSELRVDGGDACNSACGAFGTPEYCCSGAFGSPSSCSPSVYSE 67
+G C C D+N +CP+ELR GG CN+ C A+ T E+CC+ G +C P+ +
Sbjct: 440 NGGCHKISCTADINGQCPNELRAPGG--CNNPCTAYKTNEFCCTNGQG---TCGPN*FLR 604
Query: 68 MFKSACPKS 76
FK C S
Sbjct: 605 FFKDRCHDS 631
>TC19049 similar to PIR|T05694|T05694 pathogenesis-related protein
F20M13.220 - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (36%)
Length = 861
Score = 53.5 bits (127), Expect = 5e-09
Identities = 23/28 (82%), Positives = 24/28 (85%)
Frame = +3
Query: 1 MVETSGGSGSCQATGCGEDLNRRCPSEL 28
MV SGGSGSC ATGCG DLNR+CPSEL
Sbjct: 777 MVVASGGSGSCSATGCGADLNRKCPSEL 860
>BP056986
Length = 450
Score = 51.6 bits (122), Expect = 2e-08
Identities = 23/35 (65%), Positives = 25/35 (70%)
Frame = -3
Query: 69 FKSACPKSYSYAFDDATSTFTCTAADDYTITFCPS 103
FK ACP SYSYA+DD TS FTC+ DY I FC S
Sbjct: 445 FKEACPTSYSYAYDDPTSIFTCSGT-DYVIAFCAS 344
>TC18115 weakly similar to UP|Q93XD4 (Q93XD4) Thaumatin-like protein,
partial (63%)
Length = 792
Score = 38.9 bits (89), Expect = 1e-04
Identities = 36/110 (32%), Positives = 49/110 (43%), Gaps = 14/110 (12%)
Frame = +2
Query: 7 GSGSCQATGCGE----DLNRRCP---SELRVDGGDACN----SACGAFGTP-EYCCSGAF 54
G GSCQ CG L+ R P +E ++GG+ + S F P E+
Sbjct: 230 GRGSCQTGDCGGALSCSLSGRPPTTLAEFTLNGGNNQDYYDISVIDGFNVPMEF------ 391
Query: 55 GSPSS--CSPSVYSEMFKSACPKSYSYAFDDATSTFTCTAADDYTITFCP 102
SP+S C+ S+ K CP +Y Y DD T T +C Y + FCP
Sbjct: 392 -SPTSNGCTRSLRCHADK--CPDAYLYPSDD-TKTHSCPGGTHYRVVFCP 529
>BP057889
Length = 473
Score = 31.2 bits (69), Expect = 0.028
Identities = 14/46 (30%), Positives = 21/46 (45%)
Frame = -2
Query: 33 GDACNSACGAFGTPEYCCSGAFGSPSSCSPSVYSEMFKSACPKSYS 78
G C A P+YCC + S SC+ S ++ C +SY+
Sbjct: 433 GPLCQPAVAYVRPPKYCCIRSLSSCISCTACRSSTDLRTTCRRSYT 296
>TC9780 similar to AAQ56830 (AAQ56830) At5g09670, partial (55%)
Length = 1250
Score = 29.3 bits (64), Expect = 0.11
Identities = 19/59 (32%), Positives = 25/59 (42%), Gaps = 3/59 (5%)
Frame = +2
Query: 6 GGSGSCQATGCGEDLNRRCPSELRVDGGDACNS---ACGAFGTPEYCCSGAFGSPSSCS 61
GG C +GC + R +R GG C S A A G+ ++C A G CS
Sbjct: 1052 GGGKRCAVSGCTKSARGRTDCCVRHGGGKRCKSEGCAKSAQGSTDFC--KAHGGGKRCS 1222
Score = 26.6 bits (57), Expect = 0.68
Identities = 19/63 (30%), Positives = 23/63 (36%), Gaps = 7/63 (11%)
Frame = +2
Query: 6 GGSGSCQATGCGEDLNRRCPSELRVDGGDACNS-AC--GAFGTPEYCCSGAFGS----PS 58
GG CQ GC + R GG CN C A G +YC + G P
Sbjct: 515 GGGQRCQKPGCNKGAESRTAYCKAHGGGRRCNHLGCTKSAEGKTDYCIAHGGGKRCGYPD 694
Query: 59 SCS 61
C+
Sbjct: 695 GCT 703
>TC14715
Length = 637
Score = 28.9 bits (63), Expect = 0.14
Identities = 13/46 (28%), Positives = 19/46 (41%)
Frame = +1
Query: 33 GDACNSACGAFGTPEYCCSGAFGSPSSCSPSVYSEMFKSACPKSYS 78
G C A +YCC + S SC+ S ++ C SY+
Sbjct: 181 GQLCKPAVAYVSPTKYCCIRSLSSCISCTACSSSTDLRTTCRSSYT 318
>TC12482 similar to UP|Q9TD47 (Q9TD47) NADH dehydrogenase subunit II,
partial (5%)
Length = 720
Score = 28.5 bits (62), Expect = 0.18
Identities = 10/20 (50%), Positives = 13/20 (65%)
Frame = -1
Query: 49 CCSGAFGSPSSCSPSVYSEM 68
CC G+F PSSC P + + M
Sbjct: 204 CCEGSFEVPSSCFPLIITSM 145
>TC18368 similar to GB|AAP13435.1|30023804|BT006327 At1g20030 {Arabidopsis
thaliana;}, partial (7%)
Length = 603
Score = 27.7 bits (60), Expect = 0.31
Identities = 12/15 (80%), Positives = 13/15 (86%)
Frame = +3
Query: 89 TCTAADDYTITFCPS 103
TC +AD YTITFCPS
Sbjct: 3 TCASAD-YTITFCPS 44
>TC14830 similar to UP|Q8GVF6 (Q8GVF6) Pyrrolidone carboxyl peptidase-like
protein, partial (91%)
Length = 1088
Score = 27.3 bits (59), Expect = 0.40
Identities = 17/54 (31%), Positives = 26/54 (47%), Gaps = 9/54 (16%)
Frame = -3
Query: 51 SGAFGSPSSCSPSVYSEMFKSACPKS---------YSYAFDDATSTFTCTAADD 95
SG+F SPS CS ++ F+S S Y+ + + +STF C A +
Sbjct: 1008 SGSFASPSKCSWLSHTCHFQSTVSIS*QYLIITIIYNISLKEHSSTFICIATPE 847
>BP078538
Length = 511
Score = 27.3 bits (59), Expect = 0.40
Identities = 16/46 (34%), Positives = 22/46 (47%)
Frame = -3
Query: 42 AFGTPEYCCSGAFGSPSSCSPSVYSEMFKSACPKSYSYAFDDATST 87
+F YCC G G S S + + FK+A K D+A+ST
Sbjct: 506 SFSNNRYCCIGGNGGGESSSEASTAYSFKNAIVKD----SDEASST 381
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.316 0.130 0.422
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,582,247
Number of Sequences: 28460
Number of extensions: 40051
Number of successful extensions: 274
Number of sequences better than 10.0: 86
Number of HSP's better than 10.0 without gapping: 262
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 263
length of query: 107
length of database: 4,897,600
effective HSP length: 83
effective length of query: 24
effective length of database: 2,535,420
effective search space: 60850080
effective search space used: 60850080
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 47 (22.7 bits)
Medicago: description of AC121233.12