BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC149582.7 - phase: 0
(155 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC211379 similar to UP|Q8S901 (Q8S901) Syringolide-induced prote... 77 4e-15
TC231428 UP|Q8S901 (Q8S901) Syringolide-induced protein 14-1-1, ... 74 2e-14
BU090741 homologue to GP|19911575|dbj syringolide-induced protei... 50 3e-07
TC209935 43 5e-05
TC229195 similar to UP|Q9SJN3 (Q9SJN3) Expressed protein (At2g36... 41 2e-04
TC220904 homologue to GB|AAS47665.1|44681450|BT011659 At5g03110 ... 37 0.003
AW569068 36 0.007
TC204653 similar to UP|Q9AT20 (Q9AT20) Histone H1 (Fragment), pa... 35 0.019
TC223114 34 0.025
TC213832 similar to UP|AGA1_YEAST (P32323) A-agglutinin attachme... 34 0.025
TC212838 32 0.094
TC211959 homologue to PIR|E86488|E86488 protein T32E20.35 [impor... 32 0.12
TC215490 weakly similar to UP|Q8I7C3 (Q8I7C3) Lasp protein, part... 31 0.21
CD413771 30 0.61
TC229856 similar to UP|Q7Z615 (Q7Z615) Specificity protein 8 sho... 30 0.61
BU545346 29 0.79
TC230271 29 0.79
TC218402 28 1.4
TC227725 28 1.8
CO978729 27 5.2
>TC211379 similar to UP|Q8S901 (Q8S901) Syringolide-induced protein 14-1-1,
partial (16%)
Length = 457
Score = 76.6 bits (187), Expect = 4e-15
Identities = 43/93 (46%), Positives = 55/93 (58%), Gaps = 8/93 (8%)
Frame = +3
Query: 2 EKLSKMKNKFFKFLPKRPVASVS-SYQNPTLSPNASVTTARKVS-------IIPKEARRK 53
EK SK+KNK FK LPK A+VS ++QNP SP +IP EARRK
Sbjct: 42 EKESKLKNKIFKILPKAAAAAVSMTFQNPPFSPGRDHKFKHHGGKGFFSGPMIPVEARRK 221
Query: 54 HRSISFSAREPSSPKVSCMGQVKSKKKKRKAKR 86
+ +EP+SPK+SCMGQ+K KKKK + K+
Sbjct: 222 SKDGCVETQEPTSPKISCMGQIKHKKKKNQIKK 320
>TC231428 UP|Q8S901 (Q8S901) Syringolide-induced protein 14-1-1, complete
Length = 1150
Score = 74.3 bits (181), Expect = 2e-14
Identities = 58/183 (31%), Positives = 88/183 (47%), Gaps = 35/183 (19%)
Frame = +1
Query: 2 EKLSKMKNKFFKFLPKRPVASVSSYQNPTLSPNASVTTARKVSIIPKEARRKHRSISFS- 60
E +MK+K K LPK A ++QN SP + VS+IP EARRK +
Sbjct: 109 ETAYRMKSKILKALPKAVAAVTVTFQNHPFSPGRDHKSKPIVSMIPHEARRKTHDRGNNG 288
Query: 61 -----AREPSSPKVSCMGQVKSKKKK-RKAKRIHQSSTKNNDSVTSHENK---------- 104
++EP+SPK+SCMGQ+K KKK+ +K+K +S TK +V S +
Sbjct: 289 IDEIYSQEPTSPKISCMGQIKHKKKQIKKSKSKSKSMTKEARNVASKKTSSASRDIEVKK 468
Query: 105 ------KILL-----------QSEKALVVEENQEFA-PSMLIVPSLGTMKKFESGRGSLS 146
K+LL +S + + N++ A P + M++F SGR +L+
Sbjct: 469 HVSTFHKMLLFHGAKPKSEGRKSNASAPGDINKDLALADAARAPRVSQMRRFSSGRDALA 648
Query: 147 DFD 149
+FD
Sbjct: 649 EFD 657
>BU090741 homologue to GP|19911575|dbj syringolide-induced protein 14-1-1
{Glycine max}, partial (12%)
Length = 401
Score = 50.4 bits (119), Expect = 3e-07
Identities = 35/104 (33%), Positives = 52/104 (49%), Gaps = 5/104 (4%)
Frame = +3
Query: 51 RRKHRSISFSAREPSSPKVSCMGQVKSKKKKRKAKRIHQSSTKNNDSVTSHENK--KILL 108
R+ +EP+SPK+SCMGQ+K KK K + T + V H +K ++LL
Sbjct: 6 RKAKNGHGVETQEPTSPKISCMGQIKHKKGGAKNMSM-SMPTPRDPEVKKHASKFQRMLL 182
Query: 109 ---QSEKALVVEENQEFAPSMLIVPSLGTMKKFESGRGSLSDFD 149
K + E AP+ P +G M++F SGR + S+FD
Sbjct: 183 FHAGKPKQSDADVESERAPA----PPMGHMRRFASGRETFSNFD 302
>TC209935
Length = 850
Score = 43.1 bits (100), Expect = 5e-05
Identities = 26/77 (33%), Positives = 39/77 (49%), Gaps = 16/77 (20%)
Frame = +3
Query: 41 RKVSIIPKEARRKH-----------RSISFSARE-----PSSPKVSCMGQVKSKKKKRKA 84
+K S + + RR H RS+S E PSSPKVSCMG+V+SK+ + +
Sbjct: 444 QKTSFVDVKNRRNHNKPSNIRLFPKRSVSVGKSESGLVEPSSPKVSCMGRVRSKRDRNRK 623
Query: 85 KRIHQSSTKNNDSVTSH 101
R + S+ + D+ H
Sbjct: 624 LRSSRKSSPDTDAKEKH 674
>TC229195 similar to UP|Q9SJN3 (Q9SJN3) Expressed protein
(At2g36220/F2H17.17), partial (18%)
Length = 1194
Score = 41.2 bits (95), Expect = 2e-04
Identities = 19/56 (33%), Positives = 32/56 (56%)
Frame = +3
Query: 45 IIPKEARRKHRSISFSAREPSSPKVSCMGQVKSKKKKRKAKRIHQSSTKNNDSVTS 100
+ PK + + S EPSSPKVSC+G+V+SK+ + + R Q S ++ + +
Sbjct: 564 LFPKRSASVGGKLEGSVVEPSSPKVSCIGRVRSKRDRNRRLRTRQRSISSSTATAA 731
>TC220904 homologue to GB|AAS47665.1|44681450|BT011659 At5g03110 {Arabidopsis
thaliana;} , partial (7%)
Length = 708
Score = 37.4 bits (85), Expect = 0.003
Identities = 16/32 (50%), Positives = 22/32 (68%)
Frame = +3
Query: 52 RKHRSISFSAREPSSPKVSCMGQVKSKKKKRK 83
RK + + +EPSSPKV+CMGQV+ K+ K
Sbjct: 306 RKKNTNNIETQEPSSPKVTCMGQVRVKRSASK 401
>AW569068
Length = 389
Score = 36.2 bits (82), Expect = 0.007
Identities = 22/80 (27%), Positives = 39/80 (48%), Gaps = 10/80 (12%)
Frame = -2
Query: 37 VTTARKVSIIPKEARRKHRSIS----------FSAREPSSPKVSCMGQVKSKKKKRKAKR 86
+ + RK+ I KE R+ S + F + SS + + KKKK+K++R
Sbjct: 247 ILSKRKLLFIQKEIRKSRSSTA*RTAISKTNKFKTEKNSSKSIQSQKEN*KKKKKKKSRR 68
Query: 87 IHQSSTKNNDSVTSHENKKI 106
+H S+ + S T EN+++
Sbjct: 67 LHCSA*RTGISKTELENQRV 8
>TC204653 similar to UP|Q9AT20 (Q9AT20) Histone H1 (Fragment), partial (68%)
Length = 1501
Score = 34.7 bits (78), Expect = 0.019
Identities = 27/118 (22%), Positives = 47/118 (38%), Gaps = 6/118 (5%)
Frame = +1
Query: 13 KFLPKRPVASVSSYQNPTLSPNASVTTARKVSIIPKEARRKHRSISFSAREPSSPKVSCM 72
+FL +RP+ S S + P +PN+ + A + + E + + E PK
Sbjct: 28 QFLLQRPLTSRSQFSFPVSNPNSLIPMASEEPTVAVEPAPEPATAEPPPEEKEEPKPEAK 207
Query: 73 GQVKSKKKKRKAKRIHQSSTKNN------DSVTSHENKKILLQSEKALVVEENQEFAP 124
+ + K +K R T + D++TS + K Q A +EE + P
Sbjct: 208 AKKTKEPKPKKVSRPRNPPTHPSYEEMIKDAITSLKEKTGSSQHAIAKFIEEKHKQLP 381
>TC223114
Length = 568
Score = 34.3 bits (77), Expect = 0.025
Identities = 29/110 (26%), Positives = 53/110 (47%), Gaps = 1/110 (0%)
Frame = +3
Query: 18 RPVASVSSYQNPTLSPNASVTTARKVS-IIPKEARRKHRSISFSAREPSSPKVSCMGQVK 76
R +S+ S ++ + + V+TA S I+ E RRKHR+ S R+ + S +
Sbjct: 231 RDNSSLLSEESSSRTAERGVSTAHSPSTILTGENRRKHRNAFASPRKHRNVFASTRNEYS 410
Query: 77 SKKKKRKAKRIHQSSTKNNDSVTSHENKKILLQSEKALVVEENQEFAPSM 126
+K KK ++S ++ + SH++ IL + A + +E PS+
Sbjct: 411 TKDKK------YRSMPNSSKRMPSHDSNSILQEELDAHNSWQFEEINPSV 542
>TC213832 similar to UP|AGA1_YEAST (P32323) A-agglutinin attachment subunit
precursor, partial (4%)
Length = 751
Score = 34.3 bits (77), Expect = 0.025
Identities = 19/53 (35%), Positives = 31/53 (57%)
Frame = +3
Query: 53 KHRSISFSAREPSSPKVSCMGQVKSKKKKRKAKRIHQSSTKNNDSVTSHENKK 105
K +S+ EP+SPKV+C GQ+K + K A R QS + + + + +N+K
Sbjct: 222 KTKSMGSEISEPTSPKVTCAGQMK-VRPKTTACRSWQSVMEEIEKIHTDKNQK 377
>TC212838
Length = 460
Score = 32.3 bits (72), Expect = 0.094
Identities = 20/66 (30%), Positives = 26/66 (39%)
Frame = -1
Query: 8 KNKFFKFLPKRPVASVSSYQNPTLSPNASVTTARKVSIIPKEARRKHRSISFSAREPSSP 67
KNK F FLPK P + NP + P+ + I K R SI +P
Sbjct: 382 KNKGFFFLPKPPKKRNKTDSNPRIPPDTKTWAIFVGNRITKTTRMVPVSIKERQEDPKQT 203
Query: 68 KVSCMG 73
C+G
Sbjct: 202 NPQCLG 185
>TC211959 homologue to PIR|E86488|E86488 protein T32E20.35 [imported] -
Arabidopsis thaliana {Arabidopsis thaliana;} , partial
(19%)
Length = 894
Score = 32.0 bits (71), Expect = 0.12
Identities = 21/55 (38%), Positives = 27/55 (48%)
Frame = +3
Query: 26 YQNPTLSPNASVTTARKVSIIPKEARRKHRSISFSAREPSSPKVSCMGQVKSKKK 80
YQ T P + K +P R + S S S +P SP++SCMGQVK K
Sbjct: 81 YQKSTFLPMLCSRPSIKEVTLP---RFRDPSAS-SGNDPLSPRISCMGQVKRNNK 233
>TC215490 weakly similar to UP|Q8I7C3 (Q8I7C3) Lasp protein, partial (7%)
Length = 715
Score = 31.2 bits (69), Expect = 0.21
Identities = 22/91 (24%), Positives = 37/91 (40%), Gaps = 11/91 (12%)
Frame = +3
Query: 52 RKHRSISFSAREPSSPKVSCMGQVKSKKKKRK---AKRIHQSSTKNN--------DSVTS 100
R + R+P +P+ Q +++ + + A + HQ ST DSV+S
Sbjct: 6 RSDHGVPVGYRKPPTPQPQVQSQTQAQSQAQTQTLAPQFHQKSTAGGAVVDLPSPDSVSS 185
Query: 101 HENKKILLQSEKALVVEENQEFAPSMLIVPS 131
+ + KA V +E + P VPS
Sbjct: 186 DSSLANAISRSKAAVYQEQAQIQPGTTRVPS 278
>CD413771
Length = 679
Score = 29.6 bits (65), Expect = 0.61
Identities = 17/36 (47%), Positives = 21/36 (58%)
Frame = +1
Query: 75 VKSKKKKRKAKRIHQSSTKNNDSVTSHENKKILLQS 110
VK KKKK+K KR + KN+ V S + I LQS
Sbjct: 43 VKKKKKKKKKKRKKKKKKKNSRLVLSLQQGAIDLQS 150
>TC229856 similar to UP|Q7Z615 (Q7Z615) Specificity protein 8 short isoform,
partial (5%)
Length = 821
Score = 29.6 bits (65), Expect = 0.61
Identities = 17/48 (35%), Positives = 20/48 (41%)
Frame = +2
Query: 29 PTLSPNASVTTARKVSIIPKEARRKHRSISFSAREPSSPKVSCMGQVK 76
P LSP +V A EA +H + F PSS C G VK
Sbjct: 464 PALSPLPNVAFAHAEPPPKNEAAAEHLNSHFHGPSPSSSSAGCAGTVK 607
>BU545346
Length = 539
Score = 29.3 bits (64), Expect = 0.79
Identities = 17/58 (29%), Positives = 32/58 (54%)
Frame = -2
Query: 75 VKSKKKKRKAKRIHQSSTKNNDSVTSHENKKILLQSEKALVVEENQEFAPSMLIVPSL 132
+ +K K + +R+H + N +K+LLQ +KAL + + Q +P++L +P L
Sbjct: 352 LNTKLKG*RRRRVHPRLIRGNRIC---HTRKMLLQGKKALHLWQQQTPSPTILFLPPL 188
>TC230271
Length = 700
Score = 29.3 bits (64), Expect = 0.79
Identities = 21/75 (28%), Positives = 34/75 (45%), Gaps = 3/75 (4%)
Frame = +2
Query: 32 SPNASVTTARKVSIIPKEARRKHRSISFSAREPSSPKV---SCMGQVKSKKKKRKAKRIH 88
SP+ K+ ++P +A + I F PS P S + S KKKRK H
Sbjct: 281 SPSTQDGVVPKIVVVPPDADLQRGKIYFLMPLPSPPSEKNSSHRQRSSSGKKKRKE---H 451
Query: 89 QSSTKNNDSVTSHEN 103
+ +NN++ ++ N
Sbjct: 452 HNDNRNNNNNNNNNN 496
>TC218402
Length = 529
Score = 28.5 bits (62), Expect = 1.4
Identities = 17/37 (45%), Positives = 22/37 (58%)
Frame = -1
Query: 11 FFKFLPKRPVASVSSYQNPTLSPNASVTTARKVSIIP 47
FFKFLP+ V S S LSPN + +R VS++P
Sbjct: 379 FFKFLPRGMVKSSS------LSPNNCSSCSRIVSLLP 287
>TC227725
Length = 1536
Score = 28.1 bits (61), Expect = 1.8
Identities = 17/55 (30%), Positives = 27/55 (48%), Gaps = 1/55 (1%)
Frame = +3
Query: 36 SVTTARKVSIIPKEARRKHRSI-SFSAREPSSPKVSCMGQVKSKKKKRKAKRIHQ 89
S+ R II K RR +++ S+ E K+SC+ +SK+ K +HQ
Sbjct: 558 SLMLHRHYQIILKRQRRSQKAMRSYQFLEKYMSKMSCLPITRSKQSTMGTKMLHQ 722
>CO978729
Length = 377
Score = 26.6 bits (57), Expect = 5.2
Identities = 16/47 (34%), Positives = 21/47 (44%)
Frame = +1
Query: 66 SPKVSCMGQVKSKKKKRKAKRIHQSSTKNNDSVTSHENKKILLQSEK 112
S +C G+ K KKKK+K K+ + K KK Q EK
Sbjct: 91 SKAFTCKGKKKKKKKKKKKKKKKKKPKKKKKKKKKKXXKK---QKEK 222
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.310 0.123 0.323
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,378,829
Number of Sequences: 63676
Number of extensions: 62878
Number of successful extensions: 660
Number of sequences better than 10.0: 72
Number of HSP's better than 10.0 without gapping: 640
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 653
length of query: 155
length of database: 12,639,632
effective HSP length: 89
effective length of query: 66
effective length of database: 6,972,468
effective search space: 460182888
effective search space used: 460182888
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 55 (25.8 bits)
Medicago: description of AC149582.7