BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC149582.6 + phase: 0
(377 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BF325009 49 5e-06
BU547704 48 8e-06
TC208945 similar to UP|Q9FHZ3 (Q9FHZ3) Gb|AAF34839.1 (AT5g53830/... 32 0.47
TC226884 similar to GB|AAO63346.1|28950845|BT005282 At1g67325 {A... 31 0.80
TC219357 similar to UP|Q9LNL6 (Q9LNL6) F12K21.12, partial (43%) 30 1.4
TC211473 weakly similar to UP|Q84S70 (Q84S70) Kelch-like protein... 30 1.4
TC219363 similar to UP|Q9FZA1 (Q9FZA1) F3H9.7 protein, partial (... 30 1.8
TC234898 30 2.3
TC224386 weakly similar to UP|Q9MAL4 (Q9MAL4) F27F5.2, partial (... 30 2.3
TC218296 29 3.0
TC205515 similar to UP|Q84TL1 (Q84TL1) Glycine decarboxylase com... 29 4.0
TC207022 weakly similar to UP|Q8LGS1 (Q8LGS1) Senescence-associa... 29 4.0
TC225514 homologue to UP|KSG9_ARATH (Q39012) Shaggy-related prot... 28 5.2
TC230722 weakly similar to UP|Q9LK73 (Q9LK73) Flavonol 3-O-gluco... 28 6.8
TC228341 homologue to UP|O24422 (O24422) Desiccation protective ... 28 6.8
BI787676 28 6.8
TC209691 28 6.8
TC205513 similar to UP|Q84TL1 (Q84TL1) Glycine decarboxylase com... 28 6.8
CA784474 homologue to SP|Q39261|ZFP2_ Zinc finger protein 2. [Mo... 28 8.9
TC233196 28 8.9
>BF325009
Length = 382
Score = 48.5 bits (114), Expect = 5e-06
Identities = 39/108 (36%), Positives = 49/108 (45%), Gaps = 7/108 (6%)
Frame = +2
Query: 212 VIVFKGLFYVTDRWGTISWIDISSLRLIQFAPPLCGFGNKKHLVESCGNLYVVDRYYEGE 271
V VFKG Y D G + L A P+ G G+KK LVES G L +VD Y
Sbjct: 2 VCVFKGNLYGADSNGRTVRVRPDDGGLTLAAEPVFG-GDKKFLVESEGALLLVDMYLSYY 178
Query: 272 NMRSNNVGRHHDQDAVVEC-------FKVHMLDEEWGKWVDVKNLRDR 312
+ D++ V F V LDEE KWV++ +LRDR
Sbjct: 179 SCTQGLFHEDFDEEDVAGMGWERTVKFDVFRLDEEGKKWVELTDLRDR 322
>BU547704
Length = 600
Score = 47.8 bits (112), Expect = 8e-06
Identities = 23/51 (45%), Positives = 31/51 (60%)
Frame = -2
Query: 291 FKVHMLDEEWGKWVDVKNLRDRAFVLSRSCNFSVSAKELIGYQGNCIYFKD 341
F V LDEE KWV++ +L +R L C FS SAK+L +GNC+ +D
Sbjct: 554 FDVFXLDEEGKKWVELTDLGERVLFLGDDCAFSASAKDLNLGRGNCVAXRD 402
>TC208945 similar to UP|Q9FHZ3 (Q9FHZ3) Gb|AAF34839.1 (AT5g53830/MGN6_22),
partial (35%)
Length = 1127
Score = 32.0 bits (71), Expect = 0.47
Identities = 21/64 (32%), Positives = 36/64 (55%), Gaps = 6/64 (9%)
Frame = +3
Query: 75 IEPTNGSS-NFNLPPL--APSYTKGWLIKVEESNNNPLKSLL---SPISDCKLSYPLDSN 128
++P + SS NFN+PP+ P +G+ + +NNN LK+ L + + + S S+
Sbjct: 18 VQPQSSSSKNFNIPPMKTTPKKQQGFKLYERRNNNNHLKNTLMINTLVPNFAHSSGFSSS 197
Query: 129 NSSP 132
+SSP
Sbjct: 198 SSSP 209
>TC226884 similar to GB|AAO63346.1|28950845|BT005282 At1g67325 {Arabidopsis
thaliana;} , partial (49%)
Length = 503
Score = 31.2 bits (69), Expect = 0.80
Identities = 28/75 (37%), Positives = 35/75 (46%), Gaps = 9/75 (12%)
Frame = +3
Query: 31 FRSVCESFRSSIP-PSHPNSPSF--PLQIPHTIFDSLYYLHQST---IYLIEPTNGSSNF 84
FR+ C + P P+ NS S PLQ P S YL +T IYL P GSS F
Sbjct: 192 FRTTCNMRNCTQPRPADHNSKSAAKPLQAPQGYSSSAPYLGSNTPSSIYLGVPPYGSSLF 371
Query: 85 N---LPPLAPSYTKG 96
N +PP ++ G
Sbjct: 372 NGSSVPPYDVPFSGG 416
>TC219357 similar to UP|Q9LNL6 (Q9LNL6) F12K21.12, partial (43%)
Length = 1578
Score = 30.4 bits (67), Expect = 1.4
Identities = 22/82 (26%), Positives = 38/82 (45%), Gaps = 4/82 (4%)
Frame = +1
Query: 135 WNLLNYRVIELCKSYTFQKTLYSPSYVSKVVFYPNSPRINVGDCVACCIFQEG----KLG 190
W ++ ++++CKS +F+ T S V V+F + + EG ++G
Sbjct: 268 WEKMDTLILQICKSPSFKVTRLSRKNVCFVMF--------TDEITIRTLSSEGHVPDRMG 423
Query: 191 FMKYGDTKWKLVDENNFFYDDV 212
F+ + WKLV N YDD+
Sbjct: 424 FIGF----WKLVVVKNLPYDDM 477
>TC211473 weakly similar to UP|Q84S70 (Q84S70) Kelch-like protein, partial
(19%)
Length = 940
Score = 30.4 bits (67), Expect = 1.4
Identities = 15/49 (30%), Positives = 29/49 (58%), Gaps = 1/49 (2%)
Frame = +3
Query: 288 VECFK-VHMLDEEWGKWVDVKNLRDRAFVLSRSCNFSVSAKELIGYQGN 335
V+CF V MLD + G+W+ +++ ++ F LS + + + + G+ GN
Sbjct: 354 VDCFSDVEMLDLDIGRWIPTRSMLEKRFALS-AVELNGAIYAIGGFDGN 497
>TC219363 similar to UP|Q9FZA1 (Q9FZA1) F3H9.7 protein, partial (44%)
Length = 1156
Score = 30.0 bits (66), Expect = 1.8
Identities = 15/38 (39%), Positives = 20/38 (52%)
Frame = +1
Query: 76 EPTNGSSNFNLPPLAPSYTKGWLIKVEESNNNPLKSLL 113
+P S NFN+PP+ K K+ E NN LK+ L
Sbjct: 451 DPPPSSRNFNIPPIKTPPKKQHGFKLYERRNNSLKNSL 564
>TC234898
Length = 461
Score = 29.6 bits (65), Expect = 2.3
Identities = 20/71 (28%), Positives = 39/71 (54%), Gaps = 1/71 (1%)
Frame = +3
Query: 86 LPPLAPSYTKGWLIKVEESNNNPLKSLLSPISDCKLSYPLDSNNSS-PIIWNLLNYRVIE 144
LPP A ++ + +++ +NNP+ + S SDC L+ +++N+ + ++++ V E
Sbjct: 213 LPPFAGAFLICYFPCMKKCHNNPIDVVHSCKSDCALTKSINANSDARDPATDMVDSCVQE 392
Query: 145 LCKSYTFQKTL 155
K Y QK L
Sbjct: 393 CHK*YLLQKCL 425
>TC224386 weakly similar to UP|Q9MAL4 (Q9MAL4) F27F5.2, partial (17%)
Length = 504
Score = 29.6 bits (65), Expect = 2.3
Identities = 15/49 (30%), Positives = 27/49 (54%)
Frame = -1
Query: 172 RINVGDCVACCIFQEGKLGFMKYGDTKWKLVDENNFFYDDVIVFKGLFY 220
+I++G C+AC FQ+ L KY + + + N + + +VF LF+
Sbjct: 168 QIDLGYCLACSFFQDSNLKLFKY*CSHYSDLA*LNCIFYERLVFMILFF 22
>TC218296
Length = 1248
Score = 29.3 bits (64), Expect = 3.0
Identities = 27/102 (26%), Positives = 52/102 (50%), Gaps = 2/102 (1%)
Frame = -3
Query: 45 SHPNSPSFPLQIPHTIFDSLYYLHQSTIYLIEPTNGSSNFNLPP-LAPSYTKGW-LIKVE 102
S+ +S +FP + +I SL+ H S +I N N+P ++PS T+ + ++ V
Sbjct: 442 SNHSSLTFPKR---SIRSSLFSSHSS---MIIGFNFDQFSNIPSTVSPSATEDFRMVGVL 281
Query: 103 ESNNNPLKSLLSPISDCKLSYPLDSNNSSPIIWNLLNYRVIE 144
S+NN ++ SYP+ + + IW+++ + V+E
Sbjct: 280 LSSNN--------LTSKTFSYPVSTTGRTVCIWSIIQFSVVE 179
>TC205515 similar to UP|Q84TL1 (Q84TL1) Glycine decarboxylase complex
H-protein, partial (98%)
Length = 699
Score = 28.9 bits (63), Expect = 4.0
Identities = 14/37 (37%), Positives = 20/37 (53%)
Frame = +2
Query: 93 YTKGWLIKVEESNNNPLKSLLSPISDCKLSYPLDSNN 129
Y GW+IKVE S++ L +L+ K DSN+
Sbjct: 395 YKDGWIIKVEMSDSGELNNLMDSEKYSKFCEEEDSNH 505
>TC207022 weakly similar to UP|Q8LGS1 (Q8LGS1) Senescence-associated protein
SAG102, partial (25%)
Length = 1373
Score = 28.9 bits (63), Expect = 4.0
Identities = 15/46 (32%), Positives = 26/46 (55%)
Frame = -3
Query: 72 IYLIEPTNGSSNFNLPPLAPSYTKGWLIKVEESNNNPLKSLLSPIS 117
++L+ +G S +P APS++K E++NNPL L+ +S
Sbjct: 771 VWLLG*VHGISRMYIPQKAPSHSKTERHHFLENHNNPLNKFLTLMS 634
>TC225514 homologue to UP|KSG9_ARATH (Q39012) Shaggy-related protein kinase
iota (ASK-iota) , partial (56%)
Length = 896
Score = 28.5 bits (62), Expect = 5.2
Identities = 21/65 (32%), Positives = 30/65 (45%), Gaps = 5/65 (7%)
Frame = +3
Query: 214 VFKGLFYVTDRWGTISWIDISSLRLI----QFAPPLCGFGNKKHLVESCGNL-YVVDRYY 268
+F+GL Y+ G I D+ L+ LC FG+ K LVE N+ Y+ RYY
Sbjct: 21 IFRGLAYIHTVPG-ICHRDVKPQNLLVDPLTHQVKLCDFGSAKVLVEGESNISYICSRYY 197
Query: 269 EGENM 273
+
Sbjct: 198 RAPEL 212
>TC230722 weakly similar to UP|Q9LK73 (Q9LK73) Flavonol
3-O-glucosyltransferase-like protein
(AT3g16520/MDC8_15), partial (15%)
Length = 723
Score = 28.1 bits (61), Expect = 6.8
Identities = 10/15 (66%), Positives = 12/15 (79%)
Frame = +2
Query: 44 PSHPNSPSFPLQIPH 58
PSHP +P+FPL PH
Sbjct: 287 PSHPPNPTFPLPNPH 331
>TC228341 homologue to UP|O24422 (O24422) Desiccation protective protein
LEA5, partial (36%)
Length = 647
Score = 28.1 bits (61), Expect = 6.8
Identities = 18/44 (40%), Positives = 23/44 (51%)
Frame = +2
Query: 28 ILRFRSVCESFRSSIPPSHPNSPSFPLQIPHTIFDSLYYLHQST 71
+LR RS SFRSSI N P+ +Q PH+ D +L T
Sbjct: 59 LLRKRSAANSFRSSI----NNKPTIQIQPPHS--DGTIFLTSQT 172
>BI787676
Length = 421
Score = 28.1 bits (61), Expect = 6.8
Identities = 20/52 (38%), Positives = 22/52 (41%)
Frame = +1
Query: 41 SIPPSHPNSPSFPLQIPHTIFDSLYYLHQSTIYLIEPTNGSSNFNLPPLAPS 92
S PPS P P FP+ P T Y +T I P S F PL PS
Sbjct: 82 SDPPSTPP*PPFPIPPPSTAATGASYPSSNTSSQIRP--DSLRFLF*PLPPS 231
>TC209691
Length = 654
Score = 28.1 bits (61), Expect = 6.8
Identities = 19/74 (25%), Positives = 36/74 (47%)
Frame = +3
Query: 260 NLYVVDRYYEGENMRSNNVGRHHDQDAVVECFKVHMLDEEWGKWVDVKNLRDRAFVLSRS 319
NL+V++RY E N R N+ + + +D + + + E D++NL D+ +++
Sbjct: 153 NLFVLERYMEDLNTRYVNIFKEYSKDIGGKDILIQNIKE------DIRNLVDQQDAITKD 314
Query: 320 CNFSVSAKELIGYQ 333
+ S K I Q
Sbjct: 315 GSDLKSWKSHISMQ 356
>TC205513 similar to UP|Q84TL1 (Q84TL1) Glycine decarboxylase complex
H-protein, complete
Length = 708
Score = 28.1 bits (61), Expect = 6.8
Identities = 14/37 (37%), Positives = 19/37 (50%)
Frame = +1
Query: 93 YTKGWLIKVEESNNNPLKSLLSPISDCKLSYPLDSNN 129
Y GW+IKVE S+N L +L+ K DS +
Sbjct: 385 YKDGWIIKVEISDNGELNNLMDSDQYSKFCEEEDSKH 495
>CA784474 homologue to SP|Q39261|ZFP2_ Zinc finger protein 2. [Mouse-ear
cress] {Arabidopsis thaliana}, partial (14%)
Length = 423
Score = 27.7 bits (60), Expect = 8.9
Identities = 13/21 (61%), Positives = 13/21 (61%)
Frame = +3
Query: 38 FRSSIPPSHPNSPSFPLQIPH 58
F SS PP P SPS PLQ H
Sbjct: 57 FSSSDPPEAPPSPSTPLQHSH 119
>TC233196
Length = 409
Score = 27.7 bits (60), Expect = 8.9
Identities = 11/24 (45%), Positives = 14/24 (57%)
Frame = +3
Query: 156 YSPSYVSKVVFYPNSPRINVGDCV 179
YSP+Y SK +F +N G CV
Sbjct: 66 YSPTYTSKTLFITQPLDLNAGGCV 137
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.322 0.140 0.452
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 22,702,928
Number of Sequences: 63676
Number of extensions: 389391
Number of successful extensions: 2115
Number of sequences better than 10.0: 47
Number of HSP's better than 10.0 without gapping: 2106
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2115
length of query: 377
length of database: 12,639,632
effective HSP length: 99
effective length of query: 278
effective length of database: 6,335,708
effective search space: 1761326824
effective search space used: 1761326824
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 60 (27.7 bits)
Medicago: description of AC149582.6