BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC149576.7 + phase: 1 /pseudo
(576 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC208963 119 3e-27
BM525744 117 1e-26
TC234242 84 1e-16
TC211617 72 9e-13
BE057981 69 4e-12
TC210791 66 4e-11
BM892478 60 2e-09
AW781991 53 3e-07
AW200845 53 3e-07
AW569215 weakly similar to GP|4914326|gb| F14N23.12 {Arabidopsis... 49 8e-06
BG882562 46 5e-05
TC207727 32 0.59
BI497714 homologue to SP|P13890|POLS Structural polyprotein (P13... 29 6.6
TC219069 29 6.6
TC213708 28 8.6
TC207890 weakly similar to GB|AAM62812.1|21553719|AY085591 3(2),... 28 8.6
>TC208963
Length = 1163
Score = 119 bits (299), Expect = 3e-27
Identities = 90/287 (31%), Positives = 132/287 (45%), Gaps = 42/287 (14%)
Frame = +1
Query: 301 NSDMPKVVVTDRDPSMMNAVANVLPDSSAILCYFHVGKNIRSRIITDCKVKQNVVVVDGQ 360
+ DMP+V+VT D ++M+AV V P SS +LC FH+ +N++++
Sbjct: 10 DDDMPQVIVTVGDIALMSAVQVVFPSSSNLLCRFHINQNVKAKC---------------- 141
Query: 361 KKIVDEESHSKLVDTIFDASEKLVESHTQELYAGNLVEFQDACKDHPKFLEYVETTTLKP 420
K IV + +L+ DA + +V S + Y L F++ C D P +YV+ T L P
Sbjct: 142 KSIVHLKEKQELM---MDAWDVVVNSPNEGEYMQRLAFFENVCLDFPILGDYVKNTWLIP 312
Query: 421 FKDKLVRAW----MEL--------------------------------------VLHLGC 438
K+K V AW M L V+HLG
Sbjct: 313 HKEKFVTAWTNQVMHLGNTATNRFAQTLYFE*FIRKNT*LIPHKEKFVTAWTNRVMHLGN 492
Query: 439 RTTNRVEGAHGVVKEYLSTSKGDLGTCSQKIDEMLANQFGEIQSSFGRSVTVLEHRYKDV 498
TNRVE H +K L SK D+ + + M+ Q EI++SF +S V+EHR+ +
Sbjct: 493 TATNRVEATHWRLKTLLQDSKEDMCSYWDAMKNMITLQHTEIEASFEKSKNVVEHRH-NT 669
Query: 499 TLYSGLGGHMSRQAMNFIFVEEARSRKTLCIKKKTCGCVQRMSYGLP 545
Y L G +SR A++ I EE KT I CGC+ R ++GLP
Sbjct: 670 PFYVKLVGFVSRSALSHI-TEEYDRVKTAGIDSSICGCIVRTTHGLP 807
>BM525744
Length = 411
Score = 117 bits (294), Expect = 1e-26
Identities = 56/124 (45%), Positives = 83/124 (66%)
Frame = +3
Query: 158 ETKKYLTNLKKKKKESITNIKRVYNECHKFKKAKRGDLTEMQYLISKLEENEYVNYVREK 217
E + L LK + T I+ +YN ++ +++G TEMQ+L+ LE ++YV + R+
Sbjct: 36 EPRNILLTLKDHNND--TTIRHIYNARQAYRSSQKGPRTEMQHLLKLLEHDQYVCWSRKV 209
Query: 218 PESQIVQDIFWTHPTSVKLFNTFPTVLIMDSTYKTNLYRMPLFEIVGVTSTYLTYSVGFA 277
+S ++DIFW HP ++KL +F TVL +D+TYK N Y++PL EIVGVTST LT+SV FA
Sbjct: 210 DDSDAIRDIFWAHPDAIKLLGSFHTVLFLDNTYKVNRYQLPLLEIVGVTSTELTFSVAFA 389
Query: 278 FMMS 281
+M S
Sbjct: 390 YMES 401
>TC234242
Length = 478
Score = 84.3 bits (207), Expect = 1e-16
Identities = 42/95 (44%), Positives = 64/95 (67%), Gaps = 5/95 (5%)
Frame = +3
Query: 197 EMQYLISKLEENEYVNYVREKPESQIVQDIFWTHPTSVKLFNTFPTVLIMDSTYKTNLYR 256
EMQ+L+ LE ++Y+++ R K E +V+DIFW HP SVKL N V ++DSTY+TN YR
Sbjct: 15 EMQHLMKFLERDQYIHWHRIKDED-VVRDIFWCHPDSVKLVNACNLVFLIDSTYRTNRYR 191
Query: 257 MPL-----FEIVGVTSTYLTYSVGFAFMMSKKEDN 286
+PL + VG+T T +T+S FA++ ++ +N
Sbjct: 192 LPLLDFVVLDFVGMTPTGMTFSASFAYVEGERVNN 296
>TC211617
Length = 534
Score = 71.6 bits (174), Expect = 9e-13
Identities = 35/84 (41%), Positives = 55/84 (64%)
Frame = -1
Query: 166 LKKKKKESITNIKRVYNECHKFKKAKRGDLTEMQYLISKLEENEYVNYVREKPESQIVQD 225
LK+ S T IK++YN + + RGD TEMQ+L+ L+ ++Y+++ R K + +V+D
Sbjct: 258 LKEHNTNSCTTIKQIYNARSAYHSSIRGDDTEMQHLMRLLKRDQYIHWHRLK-DQDVVRD 82
Query: 226 IFWTHPTSVKLFNTFPTVLIMDST 249
+FW HP +VKL NT V ++DST
Sbjct: 81 LFWCHPDAVKLCNTCHLVFLIDST 10
>BE057981
Length = 268
Score = 69.3 bits (168), Expect = 4e-12
Identities = 32/81 (39%), Positives = 54/81 (66%)
Frame = +2
Query: 206 EENEYVNYVREKPESQIVQDIFWTHPTSVKLFNTFPTVLIMDSTYKTNLYRMPLFEIVGV 265
E ++Y+++ R K E +V+D+FW H +VKL N V ++++ YKTN YR+ L + GV
Sbjct: 14 ERDQYIHWHRLKDEF-VVRDLFWCHLDAVKLCNACHLVFLINNAYKTNRYRLLLLDFAGV 190
Query: 266 TSTYLTYSVGFAFMMSKKEDN 286
T +T+SVGFA++ ++ +N
Sbjct: 191 TPARMTFSVGFAYLEGERVNN 253
>TC210791
Length = 456
Score = 66.2 bits (160), Expect = 4e-11
Identities = 33/89 (37%), Positives = 49/89 (54%)
Frame = +2
Query: 374 DTIFDASEKLVESHTQELYAGNLVEFQDACKDHPKFLEYVETTTLKPFKDKLVRAWMELV 433
D + DA + ++ S + Y L + C D P F +YV+ T L P K+K V AW++ V
Sbjct: 182 DLVMDAWDSVMNSPNEGEYMQRLTLLEKVCSDFPTFGDYVKNTWLIPHKEKFVMAWVDRV 361
Query: 434 LHLGCRTTNRVEGAHGVVKEYLSTSKGDL 462
+HLG T +R E AH ++ L S GD+
Sbjct: 362 MHLGNTTIDRFETAHWRLENLLQDSGGDM 448
Score = 52.0 bits (123), Expect = 7e-07
Identities = 26/72 (36%), Positives = 44/72 (61%)
Frame = +1
Query: 297 LLKPNSDMPKVVVTDRDPSMMNAVANVLPDSSAILCYFHVGKNIRSRIITDCKVKQNVVV 356
L+ +++MP V++T RD ++M+AV V P SS +LC FH+ KN++++ D + + +
Sbjct: 7 LIFKDNEMPPVIITVRDIALMDAVQVVFPSSSNLLCRFHISKNVKAKSQVDSSSEGEI*L 186
Query: 357 VDGQKKIVDEES 368
DG DE S
Sbjct: 187 GDGCMG*CDEFS 222
>BM892478
Length = 429
Score = 60.5 bits (145), Expect = 2e-09
Identities = 35/128 (27%), Positives = 63/128 (48%)
Frame = +3
Query: 198 MQYLISKLEENEYVNYVREKPESQIVQDIFWTHPTSVKLFNTFPTVLIMDSTYKTNLYRM 257
++Y S+ E+ Y E + +IFW S + F VL++D++Y+ +Y +
Sbjct: 33 LEYFQSRQAEDTGFFYAMEVDNGNCM-NIFWADGRSRYSCSHFGDVLVLDTSYRKTVYLV 209
Query: 258 PLFEIVGVTSTYLTYSVGFAFMMSKKEDNFTWALQMLLKLLKPNSDMPKVVVTDRDPSMM 317
P VGV +G A + + E++FTW Q L+ + + +P V+ D+D ++
Sbjct: 210 PFATFVGVNHHKQPVLLGCALIADESEESFTWLFQTWLRAM--SGRLPLTVIADQDIAIQ 383
Query: 318 NAVANVLP 325
A+A V P
Sbjct: 384 RAIAKVFP 407
>AW781991
Length = 419
Score = 53.1 bits (126), Expect = 3e-07
Identities = 30/113 (26%), Positives = 51/113 (44%), Gaps = 6/113 (5%)
Frame = +3
Query: 183 ECHKFKKAKR-----GDLT-EMQYLISKLEENEYVNYVREKPESQIVQDIFWTHPTSVKL 236
+C + + R GD+ + YL EN Y + E Q + ++FW P +
Sbjct: 66 DCRNYMRNNRLRSLEGDIQLVLDYLRQMHAENPNFFYAVQGDEDQSITNVFWADPKARMN 245
Query: 237 FNTFPTVLIMDSTYKTNLYRMPLFEIVGVTSTYLTYSVGFAFMMSKKEDNFTW 289
+ F + D+TY++N YR+P GV G AF++++ E +F W
Sbjct: 246 YTFFGDTVTFDTTYRSNRYRLPFAFFTGVNHHGQPVLFGCAFLINESEASFVW 404
>AW200845
Length = 343
Score = 53.1 bits (126), Expect = 3e-07
Identities = 27/70 (38%), Positives = 41/70 (58%)
Frame = +1
Query: 374 DTIFDASEKLVESHTQELYAGNLVEFQDACKDHPKFLEYVETTTLKPFKDKLVRAWMELV 433
D + D LV+ ++ +A +L +FQ A P F++YV T + P K+K++ AW V
Sbjct: 64 DYVMDNWGTLVDCPSEH*FAESLQKFQIA*PPWPMFVDYVNDTWIIPHKEKIITAWTNKV 243
Query: 434 LHLGCRTTNR 443
+HLG TTNR
Sbjct: 244 MHLGNTTTNR 273
>AW569215 weakly similar to GP|4914326|gb| F14N23.12 {Arabidopsis thaliana},
partial (19%)
Length = 425
Score = 48.5 bits (114), Expect = 8e-06
Identities = 25/93 (26%), Positives = 48/93 (50%)
Frame = +1
Query: 244 LIMDSTYKTNLYRMPLFEIVGVTSTYLTYSVGFAFMMSKKEDNFTWALQMLLKLLKPNSD 303
++ D+TY+ Y M L +GV + +T A + + +F+WAL+ L +K +
Sbjct: 10 VVFDTTYRVEAYDMLLGIWLGVDNNGMTCFFSCALLRDENIQSFSWALKAFLGFMKGKA- 186
Query: 304 MPKVVVTDRDPSMMNAVANVLPDSSAILCYFHV 336
P+ ++TD + + A+A LP + C +H+
Sbjct: 187 -PQTILTDHNMWLKEAIAVELPQTKHAFCIWHI 282
>BG882562
Length = 416
Score = 45.8 bits (107), Expect = 5e-05
Identities = 26/111 (23%), Positives = 52/111 (46%)
Frame = +3
Query: 213 YVREKPESQIVQDIFWTHPTSVKLFNTFPTVLIMDSTYKTNLYRMPLFEIVGVTSTYLTY 272
YV + + ++++FW S ++ F V+ DST +N Y +PL VGV +
Sbjct: 90 YVMDLNDDGQLRNVFWIDSRSRAAYSYFGDVVAFDSTCLSNNYEIPLVAFVGVNHHGKSV 269
Query: 273 SVGFAFMMSKKEDNFTWALQMLLKLLKPNSDMPKVVVTDRDPSMMNAVANV 323
+G + + + + W + L + P+ ++T+ +M +A+A V
Sbjct: 270 LLGCGLLADETFETYIWLFRAWLTCM--TGRPPQTIITNXCKAMQSAIAEV 416
>TC207727
Length = 775
Score = 32.3 bits (72), Expect = 0.59
Identities = 21/69 (30%), Positives = 37/69 (53%), Gaps = 3/69 (4%)
Frame = +2
Query: 381 EKLVESHTQE-LYAGNLVEF-QDACKDHPKFLEYVETTTL-KPFKDKLVRAWMELVLHLG 437
+ ++E++ + L GN +F ++ C D+PK EY+E L P ++ + EL+ L
Sbjct: 104 QNMIEANPSDALLLGNYAKFLKEVCDDYPKSKEYLERAILANPDDGHILSLYAELIWQLE 283
Query: 438 CRTTNRVEG 446
+ NR EG
Sbjct: 284 -KDANRAEG 307
>BI497714 homologue to SP|P13890|POLS Structural polyprotein (P130)
[Contains: Coat protein C (EC 3.4.21.-) (Capsid protein
C); Spike, partial (1%)
Length = 433
Score = 28.9 bits (63), Expect = 6.6
Identities = 16/59 (27%), Positives = 31/59 (52%)
Frame = +2
Query: 167 KKKKKESITNIKRVYNECHKFKKAKRGDLTEMQYLISKLEENEYVNYVREKPESQIVQD 225
+KKKK N K+ + H +AK+ ++ + +++EN+ + RE ++ VQD
Sbjct: 11 RKKKKRKRVNDKKKQQQDHMKIQAKKEEIKIQRRATDEVQENKKIIDERENHKNLSVQD 187
>TC219069
Length = 1045
Score = 28.9 bits (63), Expect = 6.6
Identities = 16/46 (34%), Positives = 21/46 (44%)
Frame = -1
Query: 251 KTNLYRMPLFEIVGVTSTYLTYSVGFAFMMSKKEDNFTWALQMLLK 296
K LYR L EI+ + + YS F M+K TW +L K
Sbjct: 487 KIYLYRFYLIEIIEIEMNFFLYSEKKMFQMNKNLFGGTWNKALLSK 350
>TC213708
Length = 430
Score = 28.5 bits (62), Expect = 8.6
Identities = 29/92 (31%), Positives = 43/92 (46%), Gaps = 4/92 (4%)
Frame = -3
Query: 167 KKKKKESITNIKRVYNECHKFKKAKRGDLTE-MQYLISKLEENEYVNYVREKPESQIVQD 225
KKK K IKR NE K + + + + T+ M + S + + NY ++KP +I +
Sbjct: 329 KKKIKNRNKKIKRSVNEISKPRNSNKQERTKSMSFQASTSK*SISQNY-KQKPAKKIDRS 153
Query: 226 ---IFWTHPTSVKLFNTFPTVLIMDSTYKTNL 254
IFW ++ FN F T T K NL
Sbjct: 152 RFFIFW----NLFPFNFFKTQTKRKRTRKLNL 69
>TC207890 weakly similar to GB|AAM62812.1|21553719|AY085591
3(2),5-BISPHOSPHATE NUCLEOTIDASE-like protein
{Arabidopsis thaliana;} , partial (42%)
Length = 1041
Score = 28.5 bits (62), Expect = 8.6
Identities = 23/102 (22%), Positives = 42/102 (40%), Gaps = 1/102 (0%)
Frame = -1
Query: 201 LISKLEENEYVNYVREKPESQIVQDIFWTHPTSVKLFNTFPTVLIMDST-YKTNLYRMPL 259
L + N +N++ P+ + + H + NT + + D YK+ LY +
Sbjct: 657 LYQRQPSNTCINFLNMLPKEEFDCSLHCQHCLGH*IRNTVAVIPMFDYLEYKSLLYVLCQ 478
Query: 260 FEIVGVTSTYLTYSVGFAFMMSKKEDNFTWALQMLLKLLKPN 301
+ ST+ VG++ SK + +LQ L+ PN
Sbjct: 477 THQQNIWSTHKVI*VGYSNFSSKFLSRYEQSLQFLIHQENPN 352
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.334 0.144 0.457
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 23,896,090
Number of Sequences: 63676
Number of extensions: 316573
Number of successful extensions: 2428
Number of sequences better than 10.0: 32
Number of HSP's better than 10.0 without gapping: 2404
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2423
length of query: 576
length of database: 12,639,632
effective HSP length: 102
effective length of query: 474
effective length of database: 6,144,680
effective search space: 2912578320
effective search space used: 2912578320
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.6 bits)
S2: 62 (28.5 bits)
Medicago: description of AC149576.7