BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC149574.9 + phase: 2 /pseudo
(88 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC229493 similar to UP|Q8LBJ5 (Q8LBJ5) Nucleic acid binding prot... 116 2e-27
TC206438 similar to UP|Q8LBJ5 (Q8LBJ5) Nucleic acid binding prot... 93 3e-20
TC206439 similar to UP|Q8LBJ5 (Q8LBJ5) Nucleic acid binding prot... 87 2e-18
TC208334 similar to UP|Q8LBJ5 (Q8LBJ5) Nucleic acid binding prot... 71 2e-17
TC205039 similar to UP|Q9FFF5 (Q9FFF5) Nucleic acid binding prot... 60 1e-10
AW277555 similar to GP|21592780|gb nucleic acid binding protein-... 57 1e-09
TC219840 similar to UP|Q9M2B4 (Q9M2B4) Nucleic acid binding prot... 53 3e-08
TC214689 homologue to UP|Q40359 (Q40359) Alfin-1, partial (95%) 52 5e-08
TC205042 similar to UP|Q9FFF5 (Q9FFF5) Nucleic acid binding prot... 52 5e-08
TC205041 similar to GB|AAN28771.1|23505823|AY143832 At3g11200/F1... 47 2e-06
TC205040 similar to UP|Q9FFF5 (Q9FFF5) Nucleic acid binding prot... 41 1e-04
TC207914 33 0.032
TC214278 similar to UP|P93704 (P93704) HMG-1, partial (98%) 32 0.072
BU550052 similar to GP|7291176|gb|A CG15295-PA {Drosophila melan... 31 0.12
TC229586 30 0.16
TC214307 similar to UP|P93704 (P93704) HMG-1, partial (48%) 30 0.21
TC233241 weakly similar to UP|Q940V3 (Q940V3) At2g22590/T9I22.3,... 29 0.35
TC224855 similar to UP|KHL1_HUMAN (Q9NR64) Kelch-like protein 1,... 29 0.46
TC230114 homologue to UP|Q9AT39 (Q9AT39) SHOOT1 protein, partial... 28 0.61
TC227483 UP|Q9AT39 (Q9AT39) SHOOT1 protein, complete 28 0.61
>TC229493 similar to UP|Q8LBJ5 (Q8LBJ5) Nucleic acid binding protein-like,
partial (92%)
Length = 1103
Score = 116 bits (290), Expect = 2e-27
Identities = 62/91 (68%), Positives = 71/91 (77%), Gaps = 3/91 (3%)
Frame = +2
Query: 1 KRLFTPINDLPTIFEVVTGSAKKQTKEKPSVSSHNSNKSKSGSKARGSELAKYSKPPAKE 60
KRLF+ INDLPTIFE+VTGSAKKQTKEK S+S+H+SNKSKSGSK RGSE KYSK E
Sbjct: 527 KRLFSMINDLPTIFEIVTGSAKKQTKEKSSISNHSSNKSKSGSKGRGSESGKYSKETKDE 706
Query: 61 DDE---EVDDEEEYQGECTACGENYVSASDE 88
++E E DDEE + C ACGE+Y ASDE
Sbjct: 707 EEEVLDEEDDEEHEETLCGACGEHY--ASDE 793
>TC206438 similar to UP|Q8LBJ5 (Q8LBJ5) Nucleic acid binding protein-like,
partial (93%)
Length = 1133
Score = 92.8 bits (229), Expect = 3e-20
Identities = 59/98 (60%), Positives = 71/98 (72%), Gaps = 10/98 (10%)
Frame = +3
Query: 1 KRLFTPINDLPTIFEVVTGSAKKQTKEKPSVSSHNSNKSKSGSKARGSELAKYSKP-PAK 59
KRLF INDLPTIFEVVTG AKKQ KEK SVS+H+S KSKS SK RGSE +KY+K +K
Sbjct: 492 KRLFNMINDLPTIFEVVTGMAKKQGKEKSSVSNHSSTKSKSNSK-RGSE-SKYTKAMQSK 665
Query: 60 EDDEE-------VDDEEEYQGE--CTACGENYVSASDE 88
++D+E D+EEE G+ C ACGE+Y A+DE
Sbjct: 666 DEDDEGGAGLGLEDEEEEEHGDTLCGACGESY--AADE 773
>TC206439 similar to UP|Q8LBJ5 (Q8LBJ5) Nucleic acid binding protein-like,
partial (60%)
Length = 714
Score = 86.7 bits (213), Expect = 2e-18
Identities = 57/95 (60%), Positives = 64/95 (67%), Gaps = 7/95 (7%)
Frame = +3
Query: 1 KRLFTPINDLPTIFEVVTGSAKKQTKEKPSVSSHNSNKSKSGSKARGSELAKYSK--PPA 58
KRLF INDLPTIFEVVTG K KEK SVS+H+S KSKS SK RGSE +KY+K
Sbjct: 96 KRLFNMINDLPTIFEVVTGMXKXXGKEKSSVSNHSSTKSKSNSK-RGSE-SKYTKAMQSK 269
Query: 59 KEDDEEVDDEEEYQGE-----CTACGENYVSASDE 88
EDDE V EEE + E C ACGE+Y A+DE
Sbjct: 270 DEDDEGVGVEEEDEDEHGETLCGACGESY--AADE 368
>TC208334 similar to UP|Q8LBJ5 (Q8LBJ5) Nucleic acid binding protein-like,
partial (86%)
Length = 1578
Score = 71.2 bits (173), Expect(2) = 2e-17
Identities = 35/46 (76%), Positives = 40/46 (86%)
Frame = +3
Query: 1 KRLFTPINDLPTIFEVVTGSAKKQTKEKPSVSSHNSNKSKSGSKAR 46
KRLF IN+LPTIFEVVTG+AKKQ KEK SVS+H+ +KSKS SKAR
Sbjct: 516 KRLFNMINELPTIFEVVTGAAKKQVKEKSSVSNHSGSKSKSSSKAR 653
Score = 32.7 bits (73), Expect(2) = 2e-17
Identities = 17/44 (38%), Positives = 29/44 (65%), Gaps = 3/44 (6%)
Frame = +2
Query: 42 GSKARGSELAKYSKPPAKEDDEEVDDEEEYQ-GE--CTACGENY 82
G++A S+ + K++DEE+DD+++ + GE C ACGE+Y
Sbjct: 647 GTRAPESQTRQPKPLQPKDEDEELDDQDDDEHGETLCGACGEHY 778
>TC205039 similar to UP|Q9FFF5 (Q9FFF5) Nucleic acid binding protein-like,
partial (95%)
Length = 1243
Score = 60.5 bits (145), Expect = 1e-10
Identities = 34/82 (41%), Positives = 49/82 (59%)
Frame = +3
Query: 1 KRLFTPINDLPTIFEVVTGSAKKQTKEKPSVSSHNSNKSKSGSKARGSELAKYSKPPAKE 60
KRLF+ INDLPT+FEVVT +K K+KP+ S + ++ GS R S+ S P +
Sbjct: 495 KRLFSLINDLPTVFEVVT--ERKPVKDKPTADSGSKSR---GSTKRSSDGQVKSNPKFAD 659
Query: 61 DDEEVDDEEEYQGECTACGENY 82
+ E +D+E + C +CG NY
Sbjct: 660 EGYEDEDDEHSETLCGSCGGNY 725
>AW277555 similar to GP|21592780|gb nucleic acid binding protein-like
{Arabidopsis thaliana}, partial (45%)
Length = 440
Score = 57.4 bits (137), Expect = 1e-09
Identities = 31/44 (70%), Positives = 37/44 (83%)
Frame = +1
Query: 2 RLFTPINDLPTIFEVVTGSAKKQTKEKPSVSSHNSNKSKSGSKA 45
RLF+ IN+LPTIFEVVT AKKQ KEK SVS+++ +KSKS SKA
Sbjct: 229 RLFSMINELPTIFEVVT--AKKQVKEKSSVSNNSGSKSKSNSKA 354
>TC219840 similar to UP|Q9M2B4 (Q9M2B4) Nucleic acid binding protein-like,
partial (41%)
Length = 622
Score = 52.8 bits (125), Expect = 3e-08
Identities = 32/64 (50%), Positives = 41/64 (64%), Gaps = 5/64 (7%)
Frame = +3
Query: 24 QTKEKPSVSSHNSNKSKSGSKARGSEL-AKYSKP--PAKEDD--EEVDDEEEYQGECTAC 78
+ KEK SVS+++ +KSKS SKAR SE + SKP P ED+ EE D++E C AC
Sbjct: 3 EVKEKSSVSNNSGSKSKSNSKARASETQGRQSKPLQPKDEDEGLEEEDNDEHGDTLCGAC 182
Query: 79 GENY 82
ENY
Sbjct: 183SENY 194
>TC214689 homologue to UP|Q40359 (Q40359) Alfin-1, partial (95%)
Length = 1352
Score = 52.0 bits (123), Expect = 5e-08
Identities = 29/49 (59%), Positives = 37/49 (75%)
Frame = +1
Query: 1 KRLFTPINDLPTIFEVVTGSAKKQTKEKPSVSSHNSNKSKSGSKARGSE 49
KRLF INDLPTIFE+VTGSA KQ K++P+ + +N +K KS K+ SE
Sbjct: 574 KRLFQMINDLPTIFELVTGSA-KQLKDQPA-AHNNGSKCKSSGKSHQSE 714
Score = 40.4 bits (93), Expect = 2e-04
Identities = 25/66 (37%), Positives = 38/66 (56%), Gaps = 1/66 (1%)
Frame = +2
Query: 18 TGSAKKQTKEKPSVSSHNSNKSKSGSKARGSELAKYSKPPAKEDDEEVDDEEEYQGE-CT 76
T +++ +K K S SH S S+A+G K S PP +ED+ ++E++ QG C
Sbjct: 767 TSCSQQCSKCKSSGKSHQSE-----SQAKGM---KMSAPPKEEDESGEEEEDDEQGATCG 922
Query: 77 ACGENY 82
ACG+NY
Sbjct: 923 ACGDNY 940
>TC205042 similar to UP|Q9FFF5 (Q9FFF5) Nucleic acid binding protein-like,
partial (49%)
Length = 676
Score = 52.0 bits (123), Expect = 5e-08
Identities = 30/80 (37%), Positives = 45/80 (55%)
Frame = +3
Query: 3 LFTPINDLPTIFEVVTGSAKKQTKEKPSVSSHNSNKSKSGSKARGSELAKYSKPPAKEDD 62
LF+ INDLP++FEVVT +K +KP+ S + + GS R S+ S P ++
Sbjct: 18 LFSLINDLPSVFEVVTD--RKPVXDKPTADSGSKXR---GSAKRSSDGQVKSNPKFVDEG 182
Query: 63 EEVDDEEEYQGECTACGENY 82
E D++E + C +CG NY
Sbjct: 183YEEDEDEHNETLCGSCGGNY 242
>TC205041 similar to GB|AAN28771.1|23505823|AY143832 At3g11200/F11B9.12
{Arabidopsis thaliana;} , partial (65%)
Length = 661
Score = 46.6 bits (109), Expect = 2e-06
Identities = 30/74 (40%), Positives = 44/74 (58%)
Frame = +1
Query: 1 KRLFTPINDLPTIFEVVTGSAKKQTKEKPSVSSHNSNKSKSGSKARGSELAKYSKPPAKE 60
KRLF+ INDLP++FEVVT +K K+KP+ S + ++ GS R S+ S P K
Sbjct: 454 KRLFSLINDLPSVFEVVTD--RKPVKDKPTADSGSKSR---GSAKRSSDGQVKSNP--KF 612
Query: 61 DDEEVDDEEEYQGE 74
DE +++E+ E
Sbjct: 613 VDEGYEEDEDXHNE 654
>TC205040 similar to UP|Q9FFF5 (Q9FFF5) Nucleic acid binding protein-like,
partial (84%)
Length = 1092
Score = 40.8 bits (94), Expect = 1e-04
Identities = 24/74 (32%), Positives = 36/74 (48%), Gaps = 2/74 (2%)
Frame = +2
Query: 11 PTIFEVVTGSAKKQTKEKP--SVSSHNSNKSKSGSKARGSELAKYSKPPAKEDDEEVDDE 68
P+I E+V + K T+ KP + +S GS R S+ S P +D E +D+
Sbjct: 494 PSILELVLTATKVVTERKPVKDKPTADSGSKSRGSTKRSSDGQVKSNPKFADDGYEDEDD 673
Query: 69 EEYQGECTACGENY 82
E + C +CG NY
Sbjct: 674 EHSETLCGSCGGNY 715
>TC207914
Length = 1187
Score = 32.7 bits (73), Expect = 0.032
Identities = 18/68 (26%), Positives = 33/68 (48%)
Frame = +3
Query: 21 AKKQTKEKPSVSSHNSNKSKSGSKARGSELAKYSKPPAKEDDEEVDDEEEYQGECTACGE 80
A+++TKE+P + H SK ++A + + + E YQ EC +CG
Sbjct: 552 AEEKTKEEPDKTQHLQMTDARCSKCMDCKVAL-------REIIQAGNNERYQLECVSCGH 710
Query: 81 NYVSASDE 88
++ ++ DE
Sbjct: 711 SWYASRDE 734
>TC214278 similar to UP|P93704 (P93704) HMG-1, partial (98%)
Length = 932
Score = 31.6 bits (70), Expect = 0.072
Identities = 19/53 (35%), Positives = 28/53 (51%)
Frame = +1
Query: 16 VVTGSAKKQTKEKPSVSSHNSNKSKSGSKARGSELAKYSKPPAKEDDEEVDDE 68
V T KKQ EK ++S++N S+ S+ +K +ED+EE DDE
Sbjct: 550 VATAEKKKQEYEK-TISAYNKQLEGKNSEEDESDKSKSEVNDDEEDEEEDDDE 705
>BU550052 similar to GP|7291176|gb|A CG15295-PA {Drosophila melanogaster},
partial (4%)
Length = 703
Score = 30.8 bits (68), Expect = 0.12
Identities = 12/33 (36%), Positives = 19/33 (57%)
Frame = -1
Query: 47 GSELAKYSKPPAKEDDEEVDDEEEYQGECTACG 79
G ++ +K +E ++E +EEE EC ACG
Sbjct: 286 GGQVKDTTKEEQEETEDETGEEEEQDEECVACG 188
>TC229586
Length = 916
Score = 30.4 bits (67), Expect = 0.16
Identities = 19/69 (27%), Positives = 32/69 (45%)
Frame = +2
Query: 20 SAKKQTKEKPSVSSHNSNKSKSGSKARGSELAKYSKPPAKEDDEEVDDEEEYQGECTACG 79
+A++ TK++P S H +G++ K D + YQ ECTACG
Sbjct: 353 TAEETTKKEPDESQH---MQMTGARCHRCTEGKVGL----RDIIHAGHGDRYQLECTACG 511
Query: 80 ENYVSASDE 88
++ ++ DE
Sbjct: 512 HSWFASRDE 538
>TC214307 similar to UP|P93704 (P93704) HMG-1, partial (48%)
Length = 553
Score = 30.0 bits (66), Expect = 0.21
Identities = 17/55 (30%), Positives = 29/55 (51%)
Frame = +3
Query: 16 VVTGSAKKQTKEKPSVSSHNSNKSKSGSKARGSELAKYSKPPAKEDDEEVDDEEE 70
V T KKQ EK ++ ++N S+ S+ +K +ED+EE DD+++
Sbjct: 51 VATAEKKKQEYEK-TILAYNKKLEGKNSEEDESDKSKSEVNDDEEDEEEEDDDDD 212
>TC233241 weakly similar to UP|Q940V3 (Q940V3) At2g22590/T9I22.3, partial
(32%)
Length = 727
Score = 29.3 bits (64), Expect = 0.35
Identities = 17/50 (34%), Positives = 24/50 (48%), Gaps = 6/50 (12%)
Frame = -3
Query: 5 TPINDLPTIFEVVTGSAKKQTK------EKPSVSSHNSNKSKSGSKARGS 48
TP + LPT+ G + TK E+P + N N+SK+ RGS
Sbjct: 404 TPSSSLPTLLRSTLGLQEMLTKSEVSRSEQPPLQRSNQNESKTHQLLRGS 255
>TC224855 similar to UP|KHL1_HUMAN (Q9NR64) Kelch-like protein 1, partial
(3%)
Length = 885
Score = 28.9 bits (63), Expect = 0.46
Identities = 17/46 (36%), Positives = 25/46 (53%), Gaps = 1/46 (2%)
Frame = -3
Query: 40 KSGSKARGSELAKYSK-PPAKEDDEEVDDEEEYQGECTACGENYVS 84
K + R +L K K +E++EE +EEE + EC GEN V+
Sbjct: 265 KDEMRERKGKLQKAKKGEDEEEEEEEEKEEEEGRAECRDKGENSVA 128
>TC230114 homologue to UP|Q9AT39 (Q9AT39) SHOOT1 protein, partial (54%)
Length = 613
Score = 28.5 bits (62), Expect = 0.61
Identities = 20/72 (27%), Positives = 32/72 (43%), Gaps = 8/72 (11%)
Frame = +1
Query: 11 PTIFEVVTGSAKKQTKEKPSVSSHNSNKSKS--------GSKARGSELAKYSKPPAKEDD 62
PT+F T ++ +S+ SN S S GSE +K ++E++
Sbjct: 76 PTLFSPTTKFFSSKSPSYSGLSNCFSNTLLSRPLLFAVKASSGVGSEASKQESDKSEEEE 255
Query: 63 EEVDDEEEYQGE 74
EE + EEY+ E
Sbjct: 256 EEEEPYEEYEVE 291
>TC227483 UP|Q9AT39 (Q9AT39) SHOOT1 protein, complete
Length = 1580
Score = 28.5 bits (62), Expect = 0.61
Identities = 20/72 (27%), Positives = 32/72 (43%), Gaps = 8/72 (11%)
Frame = +1
Query: 11 PTIFEVVTGSAKKQTKEKPSVSSHNSNKSKS--------GSKARGSELAKYSKPPAKEDD 62
PT+F T ++ +S+ SN S S GSE +K ++E++
Sbjct: 373 PTLFSPTTKFFSSKSPSYSGLSNCFSNTLLSRPLLFAVKASSGVGSEASKQESDKSEEEE 552
Query: 63 EEVDDEEEYQGE 74
EE + EEY+ E
Sbjct: 553 EEEEPYEEYEVE 588
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.299 0.121 0.324
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,245,767
Number of Sequences: 63676
Number of extensions: 30483
Number of successful extensions: 374
Number of sequences better than 10.0: 89
Number of HSP's better than 10.0 without gapping: 345
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 358
length of query: 88
length of database: 12,639,632
effective HSP length: 64
effective length of query: 24
effective length of database: 8,564,368
effective search space: 205544832
effective search space used: 205544832
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 17 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 43 (21.6 bits)
S2: 52 (24.6 bits)
Medicago: description of AC149574.9